Nicotine is a potent extracellular polysaccharide inducer in Fusobacterium nucleatum biofilms
Autor(a) principal: | |
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Data de Publicação: | 2023 |
Outros Autores: | , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Brazilian journal of oral sciences (Online) |
Texto Completo: | https://periodicos.sbu.unicamp.br/ojs/index.php/bjos/article/view/8671137 |
Resumo: | The purpose of this in vitro study was to analyze the influence of nicotine on the extracellular polysaccharides in Fusobacterium nucleatum biofilm. Methods: F. nucleatum (ATCC 10953) biofilms supplemented with different concentrations of nicotine (0, 0.5, 1, 2, 4, and 8 mg/mL) were grown in two different BHI broth conditions [no sucrose and 1% sucrose]. Extracellular polysaccharides assay, pH measurements, and a spectrophotometric assay were performed. Data were submitted for ANOVA and Tukey honestly significant difference analyses (HSD) tests (α =.05). Results: Extracellular polysaccharides synthesis was influenced by an interaction between nicotine concentrations and growth medium solution containing sucrose (P<.05). The pH values declined in the sucrose-exposed biofilm were greater than in the group exposed only to nicotine (P<.05). The biofilm exposed to sucrose and nicotine had a higher total biofilm growth (P<.05) than the nicotine-treated biofilm without sucrose. Conclusions: Regardless of sucrose exposure, biofilms exposed to different nicotine concentrations influenced the amount of extracellular polysaccharides. |
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UNICAMP-8 |
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Brazilian journal of oral sciences (Online) |
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Nicotine is a potent extracellular polysaccharide inducer in Fusobacterium nucleatum biofilmsBiofilmsNicotinePolysaccharides, bacterialThe purpose of this in vitro study was to analyze the influence of nicotine on the extracellular polysaccharides in Fusobacterium nucleatum biofilm. Methods: F. nucleatum (ATCC 10953) biofilms supplemented with different concentrations of nicotine (0, 0.5, 1, 2, 4, and 8 mg/mL) were grown in two different BHI broth conditions [no sucrose and 1% sucrose]. Extracellular polysaccharides assay, pH measurements, and a spectrophotometric assay were performed. Data were submitted for ANOVA and Tukey honestly significant difference analyses (HSD) tests (α =.05). Results: Extracellular polysaccharides synthesis was influenced by an interaction between nicotine concentrations and growth medium solution containing sucrose (P<.05). The pH values declined in the sucrose-exposed biofilm were greater than in the group exposed only to nicotine (P<.05). The biofilm exposed to sucrose and nicotine had a higher total biofilm growth (P<.05) than the nicotine-treated biofilm without sucrose. Conclusions: Regardless of sucrose exposure, biofilms exposed to different nicotine concentrations influenced the amount of extracellular polysaccharides.Universidade Estadual de Campinas2023-12-12info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/otherapplication/pdfhttps://periodicos.sbu.unicamp.br/ojs/index.php/bjos/article/view/867113710.20396/bjos.v22i00.8671137Brazilian Journal of Oral Sciences; v. 22 (2023): Continuous Publication; e231137Brazilian Journal of Oral Sciences; Vol. 22 (2023): Continuous Publication; e2311371677-3225reponame:Brazilian journal of oral sciences (Online)instname:Universidade Estadual de Campinas (UNICAMP)instacron:UNICAMPenghttps://periodicos.sbu.unicamp.br/ojs/index.php/bjos/article/view/8671137/32995United States; ContemporanyCopyright (c) 2023 Adaias Oliveira Matos, Valentim Adelino Ricardo Barão, Richard Lee Gregoryhttps://creativecommons.org/licenses/by/4.0info:eu-repo/semantics/openAccessMatos, Adaias Oliveira Barão, Valentim Adelino RicardoGregory, Richard Lee2023-12-18T17:15:29Zoai:ojs.periodicos.sbu.unicamp.br:article/8671137Revistahttps://periodicos.sbu.unicamp.br/ojs/index.php/bjos/PUBhttps://periodicos.sbu.unicamp.br/ojs/index.php/bjos/oaibrjorals@fop.unicamp.br||brjorals@fop.unicamp.br1677-32251677-3217opendoar:2023-12-18T17:15:29Brazilian journal of oral sciences (Online) - Universidade Estadual de Campinas (UNICAMP)false |
dc.title.none.fl_str_mv |
Nicotine is a potent extracellular polysaccharide inducer in Fusobacterium nucleatum biofilms |
title |
Nicotine is a potent extracellular polysaccharide inducer in Fusobacterium nucleatum biofilms |
spellingShingle |
Nicotine is a potent extracellular polysaccharide inducer in Fusobacterium nucleatum biofilms Matos, Adaias Oliveira Biofilms Nicotine Polysaccharides, bacterial |
title_short |
Nicotine is a potent extracellular polysaccharide inducer in Fusobacterium nucleatum biofilms |
title_full |
Nicotine is a potent extracellular polysaccharide inducer in Fusobacterium nucleatum biofilms |
title_fullStr |
Nicotine is a potent extracellular polysaccharide inducer in Fusobacterium nucleatum biofilms |
title_full_unstemmed |
Nicotine is a potent extracellular polysaccharide inducer in Fusobacterium nucleatum biofilms |
title_sort |
Nicotine is a potent extracellular polysaccharide inducer in Fusobacterium nucleatum biofilms |
author |
Matos, Adaias Oliveira |
author_facet |
Matos, Adaias Oliveira Barão, Valentim Adelino Ricardo Gregory, Richard Lee |
author_role |
author |
author2 |
Barão, Valentim Adelino Ricardo Gregory, Richard Lee |
author2_role |
author author |
dc.contributor.author.fl_str_mv |
Matos, Adaias Oliveira Barão, Valentim Adelino Ricardo Gregory, Richard Lee |
dc.subject.por.fl_str_mv |
Biofilms Nicotine Polysaccharides, bacterial |
topic |
Biofilms Nicotine Polysaccharides, bacterial |
description |
The purpose of this in vitro study was to analyze the influence of nicotine on the extracellular polysaccharides in Fusobacterium nucleatum biofilm. Methods: F. nucleatum (ATCC 10953) biofilms supplemented with different concentrations of nicotine (0, 0.5, 1, 2, 4, and 8 mg/mL) were grown in two different BHI broth conditions [no sucrose and 1% sucrose]. Extracellular polysaccharides assay, pH measurements, and a spectrophotometric assay were performed. Data were submitted for ANOVA and Tukey honestly significant difference analyses (HSD) tests (α =.05). Results: Extracellular polysaccharides synthesis was influenced by an interaction between nicotine concentrations and growth medium solution containing sucrose (P<.05). The pH values declined in the sucrose-exposed biofilm were greater than in the group exposed only to nicotine (P<.05). The biofilm exposed to sucrose and nicotine had a higher total biofilm growth (P<.05) than the nicotine-treated biofilm without sucrose. Conclusions: Regardless of sucrose exposure, biofilms exposed to different nicotine concentrations influenced the amount of extracellular polysaccharides. |
publishDate |
2023 |
dc.date.none.fl_str_mv |
2023-12-12 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion info:eu-repo/semantics/other |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
https://periodicos.sbu.unicamp.br/ojs/index.php/bjos/article/view/8671137 10.20396/bjos.v22i00.8671137 |
url |
https://periodicos.sbu.unicamp.br/ojs/index.php/bjos/article/view/8671137 |
identifier_str_mv |
10.20396/bjos.v22i00.8671137 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
https://periodicos.sbu.unicamp.br/ojs/index.php/bjos/article/view/8671137/32995 |
dc.rights.driver.fl_str_mv |
Copyright (c) 2023 Adaias Oliveira Matos, Valentim Adelino Ricardo Barão, Richard Lee Gregory https://creativecommons.org/licenses/by/4.0 info:eu-repo/semantics/openAccess |
rights_invalid_str_mv |
Copyright (c) 2023 Adaias Oliveira Matos, Valentim Adelino Ricardo Barão, Richard Lee Gregory https://creativecommons.org/licenses/by/4.0 |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.coverage.none.fl_str_mv |
United States; Contemporany |
dc.publisher.none.fl_str_mv |
Universidade Estadual de Campinas |
publisher.none.fl_str_mv |
Universidade Estadual de Campinas |
dc.source.none.fl_str_mv |
Brazilian Journal of Oral Sciences; v. 22 (2023): Continuous Publication; e231137 Brazilian Journal of Oral Sciences; Vol. 22 (2023): Continuous Publication; e231137 1677-3225 reponame:Brazilian journal of oral sciences (Online) instname:Universidade Estadual de Campinas (UNICAMP) instacron:UNICAMP |
instname_str |
Universidade Estadual de Campinas (UNICAMP) |
instacron_str |
UNICAMP |
institution |
UNICAMP |
reponame_str |
Brazilian journal of oral sciences (Online) |
collection |
Brazilian journal of oral sciences (Online) |
repository.name.fl_str_mv |
Brazilian journal of oral sciences (Online) - Universidade Estadual de Campinas (UNICAMP) |
repository.mail.fl_str_mv |
brjorals@fop.unicamp.br||brjorals@fop.unicamp.br |
_version_ |
1800216404188725248 |