In Vitro antimicrobial photoinactivation with methylene blue in different microorganisms

• Autor(a) principal: Oliveira, Bruna Paloma de
• Data de Publicação: 2015
• Outros Autores: Lins, Carla Cabral dos Santos Accioly, Diniz, Fátima Alves, Melo, Liliane Lima, Castro, Célia Maria Machado Barbosa de
• Tipo de documento: Artigo
• Idioma: por
• Título da fonte: Brazilian journal of oral sciences (Online)
• Texto Completo: https://periodicos.sbu.unicamp.br/ojs/index.php/bjos/article/view/8640933
• topic: Endodontics; Enterococcus faecalis; Methylene blue; Microbiology; Photodynamic therapy; Odontologia

Aim: To evaluate the in vitro antimicrobial effects of photodynamic therapy (PDT). Methods: The microorganism indicators were: Candida albicans, Pseudomonas aeruginosa,Enterococcus faecalis and Staphylococcus aureus. A microbial pool was prepared (108 cells/mL), from which aliquots were transferred to culture plates for carrying out the PDT using methylene blue (50 µM) and low-power laser (660 nm, 100 mW and 9 J).The effect of methylene blue alone, low power laser and the absence of treatments were evaluated. Then, aliquots of 1 µL were plated in a media culture, the number of colony forming units (CFU/mL) was obtained and the data submitted to the F test (ANOVA) with Tamhane’s comparisons. Results:The laser radiation in the presence of methylene blue was able to eliminate 74.90% of C. albicans, 72.41% of P. aeruginosa, 96.44% of E. faecalis and 95.42% of S. aureus, thus statistically significant differences were found among the groups (p<0.001). Conclusions: PDT was effective in reducing the number of viable cells in the studiedmicroorganisms, especially E. faecalis and S. aureus.