Phenotypic characterization of Phenotypic characterization of Phenotypic characterization of Candida Candida Candida spp. isolates spp. isolates from chronic periodontitis patients from chronic periodontitis patients
Autor(a) principal: | |
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Data de Publicação: | 2016 |
Outros Autores: | , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Brazilian journal of oral sciences (Online) |
Texto Completo: | https://periodicos.sbu.unicamp.br/ojs/index.php/bjos/article/view/8642787 |
Resumo: | Aim: Several typing methods for Candida spp. have been suggested in the literature in order to distinguish isolates for studies about the virulence or infection routes of these microorganisms and, in particular, for epidemiological purposes. The aim of this study was to establish a comparison between the phenotypic profile of oral Candida isolates from periodontitis patients and control individuals. Methods: The morphotyping and biotyping of 35 C. albicans isolates obtained from chronic periodontitis patients and 48 isolates from control individuals were performed. For morphotyping, the isolates were plated on malt extract agar and incubated for 10 days. Sixteen different morphotypes were observed for C. albicans, the most frequently observed being 0000 and 0001. Results: Biotype 0000 (complete absence of fringe) was most prevalent among the isolates obtained from periodontitis patients compared to those from control individuals, with statistical significance. Biotyping revealed 5 different biotypes with higher prevalence of the biotype 357 among the isolates from control and periodontitis groups. Conclusions: The results obtained by biotyping of the isolates did not permit to differentiate a characteristic model related to periodontal disease, whilst the morphotype 0000 was most frequently isolated from periodontitis patients. |
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oai:ojs.periodicos.sbu.unicamp.br:article/8642787 |
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UNICAMP-8 |
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Brazilian journal of oral sciences (Online) |
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Phenotypic characterization of Phenotypic characterization of Phenotypic characterization of Candida Candida Candida spp. isolates spp. isolates from chronic periodontitis patients from chronic periodontitis patientsPeriodontitisCandida albicansBiotypingMorphotypingOdontologyAim: Several typing methods for Candida spp. have been suggested in the literature in order to distinguish isolates for studies about the virulence or infection routes of these microorganisms and, in particular, for epidemiological purposes. The aim of this study was to establish a comparison between the phenotypic profile of oral Candida isolates from periodontitis patients and control individuals. Methods: The morphotyping and biotyping of 35 C. albicans isolates obtained from chronic periodontitis patients and 48 isolates from control individuals were performed. For morphotyping, the isolates were plated on malt extract agar and incubated for 10 days. Sixteen different morphotypes were observed for C. albicans, the most frequently observed being 0000 and 0001. Results: Biotype 0000 (complete absence of fringe) was most prevalent among the isolates obtained from periodontitis patients compared to those from control individuals, with statistical significance. Biotyping revealed 5 different biotypes with higher prevalence of the biotype 357 among the isolates from control and periodontitis groups. Conclusions: The results obtained by biotyping of the isolates did not permit to differentiate a characteristic model related to periodontal disease, whilst the morphotype 0000 was most frequently isolated from periodontitis patients.Universidade Estadual de Campinas2016-01-15info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://periodicos.sbu.unicamp.br/ojs/index.php/bjos/article/view/864278710.20396/bjos.v7i25.8642787Brazilian Journal of Oral Sciences; v. 7 n. 25 (2008): Apr./Jun.; 1531-1534Brazilian Journal of Oral Sciences; Vol. 7 No. 25 (2008): Apr./Jun.; 1531-15341677-3225reponame:Brazilian journal of oral sciences (Online)instname:Universidade Estadual de Campinas (UNICAMP)instacron:UNICAMPenghttps://periodicos.sbu.unicamp.br/ojs/index.php/bjos/article/view/8642787/10341Koga-Ito, Cristiane YumiKomiyama, Edson YukioMartins, Clélia Aparecida de PaivaSantos, Silvana Soléo Ferreira dosBalducci, IvanJorge, Antonio Olavo Cardosoinfo:eu-repo/semantics/openAccess2016-02-25T09:25:22Zoai:ojs.periodicos.sbu.unicamp.br:article/8642787Revistahttps://periodicos.sbu.unicamp.br/ojs/index.php/bjos/PUBhttps://periodicos.sbu.unicamp.br/ojs/index.php/bjos/oaibrjorals@fop.unicamp.br||brjorals@fop.unicamp.br1677-32251677-3217opendoar:2016-02-25T09:25:22Brazilian journal of oral sciences (Online) - Universidade Estadual de Campinas (UNICAMP)false |
dc.title.none.fl_str_mv |
Phenotypic characterization of Phenotypic characterization of Phenotypic characterization of Candida Candida Candida spp. isolates spp. isolates from chronic periodontitis patients from chronic periodontitis patients |
title |
Phenotypic characterization of Phenotypic characterization of Phenotypic characterization of Candida Candida Candida spp. isolates spp. isolates from chronic periodontitis patients from chronic periodontitis patients |
spellingShingle |
Phenotypic characterization of Phenotypic characterization of Phenotypic characterization of Candida Candida Candida spp. isolates spp. isolates from chronic periodontitis patients from chronic periodontitis patients Koga-Ito, Cristiane Yumi Periodontitis Candida albicans Biotyping Morphotyping Odontology |
title_short |
Phenotypic characterization of Phenotypic characterization of Phenotypic characterization of Candida Candida Candida spp. isolates spp. isolates from chronic periodontitis patients from chronic periodontitis patients |
title_full |
Phenotypic characterization of Phenotypic characterization of Phenotypic characterization of Candida Candida Candida spp. isolates spp. isolates from chronic periodontitis patients from chronic periodontitis patients |
title_fullStr |
Phenotypic characterization of Phenotypic characterization of Phenotypic characterization of Candida Candida Candida spp. isolates spp. isolates from chronic periodontitis patients from chronic periodontitis patients |
title_full_unstemmed |
Phenotypic characterization of Phenotypic characterization of Phenotypic characterization of Candida Candida Candida spp. isolates spp. isolates from chronic periodontitis patients from chronic periodontitis patients |
title_sort |
Phenotypic characterization of Phenotypic characterization of Phenotypic characterization of Candida Candida Candida spp. isolates spp. isolates from chronic periodontitis patients from chronic periodontitis patients |
author |
Koga-Ito, Cristiane Yumi |
author_facet |
Koga-Ito, Cristiane Yumi Komiyama, Edson Yukio Martins, Clélia Aparecida de Paiva Santos, Silvana Soléo Ferreira dos Balducci, Ivan Jorge, Antonio Olavo Cardoso |
author_role |
author |
author2 |
Komiyama, Edson Yukio Martins, Clélia Aparecida de Paiva Santos, Silvana Soléo Ferreira dos Balducci, Ivan Jorge, Antonio Olavo Cardoso |
author2_role |
author author author author author |
dc.contributor.author.fl_str_mv |
Koga-Ito, Cristiane Yumi Komiyama, Edson Yukio Martins, Clélia Aparecida de Paiva Santos, Silvana Soléo Ferreira dos Balducci, Ivan Jorge, Antonio Olavo Cardoso |
dc.subject.por.fl_str_mv |
Periodontitis Candida albicans Biotyping Morphotyping Odontology |
topic |
Periodontitis Candida albicans Biotyping Morphotyping Odontology |
description |
Aim: Several typing methods for Candida spp. have been suggested in the literature in order to distinguish isolates for studies about the virulence or infection routes of these microorganisms and, in particular, for epidemiological purposes. The aim of this study was to establish a comparison between the phenotypic profile of oral Candida isolates from periodontitis patients and control individuals. Methods: The morphotyping and biotyping of 35 C. albicans isolates obtained from chronic periodontitis patients and 48 isolates from control individuals were performed. For morphotyping, the isolates were plated on malt extract agar and incubated for 10 days. Sixteen different morphotypes were observed for C. albicans, the most frequently observed being 0000 and 0001. Results: Biotype 0000 (complete absence of fringe) was most prevalent among the isolates obtained from periodontitis patients compared to those from control individuals, with statistical significance. Biotyping revealed 5 different biotypes with higher prevalence of the biotype 357 among the isolates from control and periodontitis groups. Conclusions: The results obtained by biotyping of the isolates did not permit to differentiate a characteristic model related to periodontal disease, whilst the morphotype 0000 was most frequently isolated from periodontitis patients. |
publishDate |
2016 |
dc.date.none.fl_str_mv |
2016-01-15 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
https://periodicos.sbu.unicamp.br/ojs/index.php/bjos/article/view/8642787 10.20396/bjos.v7i25.8642787 |
url |
https://periodicos.sbu.unicamp.br/ojs/index.php/bjos/article/view/8642787 |
identifier_str_mv |
10.20396/bjos.v7i25.8642787 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
https://periodicos.sbu.unicamp.br/ojs/index.php/bjos/article/view/8642787/10341 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Universidade Estadual de Campinas |
publisher.none.fl_str_mv |
Universidade Estadual de Campinas |
dc.source.none.fl_str_mv |
Brazilian Journal of Oral Sciences; v. 7 n. 25 (2008): Apr./Jun.; 1531-1534 Brazilian Journal of Oral Sciences; Vol. 7 No. 25 (2008): Apr./Jun.; 1531-1534 1677-3225 reponame:Brazilian journal of oral sciences (Online) instname:Universidade Estadual de Campinas (UNICAMP) instacron:UNICAMP |
instname_str |
Universidade Estadual de Campinas (UNICAMP) |
instacron_str |
UNICAMP |
institution |
UNICAMP |
reponame_str |
Brazilian journal of oral sciences (Online) |
collection |
Brazilian journal of oral sciences (Online) |
repository.name.fl_str_mv |
Brazilian journal of oral sciences (Online) - Universidade Estadual de Campinas (UNICAMP) |
repository.mail.fl_str_mv |
brjorals@fop.unicamp.br||brjorals@fop.unicamp.br |
_version_ |
1788167622440779776 |