CHROMOSOME BANDING IN CRUSTACEA. I. KARYOTYPE, Ag-NORs, C BANDING AND TREATMENT WITH EcoRI, PstI AND KpnI RESTRICTION ENDONUCLEASES IN Artemia franciscana

Detalhes bibliográficos
Autor(a) principal: Accioly, Ingrid Vilar
Data de Publicação: 2014
Outros Autores: Cunha, Inailson Márcio Costa da, Tavares, Joana Cristina Medeiros, Molina, Wagner Franco
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Biota Amazônia
Texto Completo: https://periodicos.unifap.br/index.php/biota/article/view/763
Resumo: Karyotypic characteristics of the microcrustacean Artemia franciscana Kellog 1906, introduced to the salt lakes on the northeastern coast of Brazil in the 1970s, were investigated by conventional staining, C banding, restriction endonucleases (EcoRI, PstI and KpnI) and Ag-NORs. The karyotype consisted of 42 chromosomes and secondary constrictions were observed on some pairs. Large heterochromatic blocks were found distributed in the telomere portion of most of the chromosomes. Digestion with PstI and KpnI showed a similar pattern to that obtained by C banding. Preparations treated with EcoRI revealed intense action in the heterochromatic regions indicating the presence of restriction sites. Multiple Ag-NORs were shown associated to heterochromatic blocks. These data presented here no identified modifications that might have occurred after the geographic isolation of this stock and examine the evolutionary modifications in the karyotype of this group.Keywords: Chromosome banding; brine shrimp; crustacean cytogenetics.
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spelling CHROMOSOME BANDING IN CRUSTACEA. I. KARYOTYPE, Ag-NORs, C BANDING AND TREATMENT WITH EcoRI, PstI AND KpnI RESTRICTION ENDONUCLEASES IN Artemia franciscanaBANDAMENTOS CROMOSSÔMICOS EM CRUSTACEA. I. CARIÓTIPO, Ag-RONs, BANDAMENTO-C E TRATAMENTO COM ENDONUCLEASES DE RESTRIÇÃO EcoRI, PstI E KpnI EM Artemia franciscanaKaryotypic characteristics of the microcrustacean Artemia franciscana Kellog 1906, introduced to the salt lakes on the northeastern coast of Brazil in the 1970s, were investigated by conventional staining, C banding, restriction endonucleases (EcoRI, PstI and KpnI) and Ag-NORs. The karyotype consisted of 42 chromosomes and secondary constrictions were observed on some pairs. Large heterochromatic blocks were found distributed in the telomere portion of most of the chromosomes. Digestion with PstI and KpnI showed a similar pattern to that obtained by C banding. Preparations treated with EcoRI revealed intense action in the heterochromatic regions indicating the presence of restriction sites. Multiple Ag-NORs were shown associated to heterochromatic blocks. These data presented here no identified modifications that might have occurred after the geographic isolation of this stock and examine the evolutionary modifications in the karyotype of this group.Keywords: Chromosome banding; brine shrimp; crustacean cytogenetics.Características cariotípicas do microcrustáceo Artemia franciscana Kellog, 1906, introduzida nas salinas do litoral nordeste do Brasil, na década de 70, foram investigadas através de coloração convencional, bandamento C, endonucleases de restrição (EcoRI, PstI e KpnI) e Ag-NORs. O cariótipo consiste de 42 cromossomos, onde se individualiza sobre alguns pares a presença de constrições secundárias. Grandes blocos heterocromáticos encontram-se distribuídos nas porções teloméricas da maioria dos cromossomos. A digestão com PstI e KpnI revelou um padrão similar ao obtido pelo bandamento C. Preparações tratadas com EcoRI apresentam digestão das regiões heterocromáticas indicando a presença de sítios de restrição nestas regiões. Ag-NORs múltiplas estão associadas a blocos heterocromáticos. Os dados apresentados representam passo inicial para identificação de possíveis modificações ocorridas após o isolamento geográfico desta amostra, assim como no entendimento das modificações evolutivas ocorridas no cariótipo deste grupo.Palavras-chave: bandamento cromossômico, camarão de água salgada, citogenética de crustáceos.Universidade Federal do AmapáCNPq, CAPES, INCAccioly, Ingrid VilarCunha, Inailson Márcio Costa daTavares, Joana Cristina MedeirosMolina, Wagner Franco2014-08-02info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionArtigo Avaliado pelos Paresapplication/pdfhttps://periodicos.unifap.br/index.php/biota/article/view/76310.18561/2179-5746/biotaamazonia.v4n2p15-19Biota Amazônia (Biote Amazonie, Biota Amazonia, Amazonian Biota); v. 4, n. 2 (2014); 15-192179-5746reponame:Biota Amazôniainstname:Universidade Federal do Amapá (UNIFAP)instacron:UNIFAPenghttps://periodicos.unifap.br/index.php/biota/article/view/763/v4n2p15-19.pdfAméricasContemporâneaEspécie invasoraDireitos autorais 2014 Biota Amazônia (Biote Amazonie, Biota Amazonia, Amazonian Biota)http://creativecommons.org/licenses/by/4.0info:eu-repo/semantics/openAccess2016-04-13T17:58:54Zoai:ojs.pkp.sfu.ca:article/763Revistahttp://periodicos.unifap.br/index.php/biotaONGhttps://periodicos.unifap.br/index.php/biota/oai||juliosa@unifap.br2179-57462179-5746opendoar:2016-04-13T17:58:54Biota Amazônia - Universidade Federal do Amapá (UNIFAP)false
dc.title.none.fl_str_mv CHROMOSOME BANDING IN CRUSTACEA. I. KARYOTYPE, Ag-NORs, C BANDING AND TREATMENT WITH EcoRI, PstI AND KpnI RESTRICTION ENDONUCLEASES IN Artemia franciscana
BANDAMENTOS CROMOSSÔMICOS EM CRUSTACEA. I. CARIÓTIPO, Ag-RONs, BANDAMENTO-C E TRATAMENTO COM ENDONUCLEASES DE RESTRIÇÃO EcoRI, PstI E KpnI EM Artemia franciscana
title CHROMOSOME BANDING IN CRUSTACEA. I. KARYOTYPE, Ag-NORs, C BANDING AND TREATMENT WITH EcoRI, PstI AND KpnI RESTRICTION ENDONUCLEASES IN Artemia franciscana
spellingShingle CHROMOSOME BANDING IN CRUSTACEA. I. KARYOTYPE, Ag-NORs, C BANDING AND TREATMENT WITH EcoRI, PstI AND KpnI RESTRICTION ENDONUCLEASES IN Artemia franciscana
Accioly, Ingrid Vilar
title_short CHROMOSOME BANDING IN CRUSTACEA. I. KARYOTYPE, Ag-NORs, C BANDING AND TREATMENT WITH EcoRI, PstI AND KpnI RESTRICTION ENDONUCLEASES IN Artemia franciscana
title_full CHROMOSOME BANDING IN CRUSTACEA. I. KARYOTYPE, Ag-NORs, C BANDING AND TREATMENT WITH EcoRI, PstI AND KpnI RESTRICTION ENDONUCLEASES IN Artemia franciscana
title_fullStr CHROMOSOME BANDING IN CRUSTACEA. I. KARYOTYPE, Ag-NORs, C BANDING AND TREATMENT WITH EcoRI, PstI AND KpnI RESTRICTION ENDONUCLEASES IN Artemia franciscana
title_full_unstemmed CHROMOSOME BANDING IN CRUSTACEA. I. KARYOTYPE, Ag-NORs, C BANDING AND TREATMENT WITH EcoRI, PstI AND KpnI RESTRICTION ENDONUCLEASES IN Artemia franciscana
title_sort CHROMOSOME BANDING IN CRUSTACEA. I. KARYOTYPE, Ag-NORs, C BANDING AND TREATMENT WITH EcoRI, PstI AND KpnI RESTRICTION ENDONUCLEASES IN Artemia franciscana
author Accioly, Ingrid Vilar
author_facet Accioly, Ingrid Vilar
Cunha, Inailson Márcio Costa da
Tavares, Joana Cristina Medeiros
Molina, Wagner Franco
author_role author
author2 Cunha, Inailson Márcio Costa da
Tavares, Joana Cristina Medeiros
Molina, Wagner Franco
author2_role author
author
author
dc.contributor.none.fl_str_mv
CNPq, CAPES, INC
dc.contributor.author.fl_str_mv Accioly, Ingrid Vilar
Cunha, Inailson Márcio Costa da
Tavares, Joana Cristina Medeiros
Molina, Wagner Franco
description Karyotypic characteristics of the microcrustacean Artemia franciscana Kellog 1906, introduced to the salt lakes on the northeastern coast of Brazil in the 1970s, were investigated by conventional staining, C banding, restriction endonucleases (EcoRI, PstI and KpnI) and Ag-NORs. The karyotype consisted of 42 chromosomes and secondary constrictions were observed on some pairs. Large heterochromatic blocks were found distributed in the telomere portion of most of the chromosomes. Digestion with PstI and KpnI showed a similar pattern to that obtained by C banding. Preparations treated with EcoRI revealed intense action in the heterochromatic regions indicating the presence of restriction sites. Multiple Ag-NORs were shown associated to heterochromatic blocks. These data presented here no identified modifications that might have occurred after the geographic isolation of this stock and examine the evolutionary modifications in the karyotype of this group.Keywords: Chromosome banding; brine shrimp; crustacean cytogenetics.
publishDate 2014
dc.date.none.fl_str_mv 2014-08-02
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Artigo Avaliado pelos Pares
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv https://periodicos.unifap.br/index.php/biota/article/view/763
10.18561/2179-5746/biotaamazonia.v4n2p15-19
url https://periodicos.unifap.br/index.php/biota/article/view/763
identifier_str_mv 10.18561/2179-5746/biotaamazonia.v4n2p15-19
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv https://periodicos.unifap.br/index.php/biota/article/view/763/v4n2p15-19.pdf
dc.rights.driver.fl_str_mv Direitos autorais 2014 Biota Amazônia (Biote Amazonie, Biota Amazonia, Amazonian Biota)
http://creativecommons.org/licenses/by/4.0
info:eu-repo/semantics/openAccess
rights_invalid_str_mv Direitos autorais 2014 Biota Amazônia (Biote Amazonie, Biota Amazonia, Amazonian Biota)
http://creativecommons.org/licenses/by/4.0
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.coverage.none.fl_str_mv


Américas
Contemporânea
Espécie invasora
dc.publisher.none.fl_str_mv Universidade Federal do Amapá
publisher.none.fl_str_mv Universidade Federal do Amapá
dc.source.none.fl_str_mv Biota Amazônia (Biote Amazonie, Biota Amazonia, Amazonian Biota); v. 4, n. 2 (2014); 15-19
2179-5746
reponame:Biota Amazônia
instname:Universidade Federal do Amapá (UNIFAP)
instacron:UNIFAP
instname_str Universidade Federal do Amapá (UNIFAP)
instacron_str UNIFAP
institution UNIFAP
reponame_str Biota Amazônia
collection Biota Amazônia
repository.name.fl_str_mv Biota Amazônia - Universidade Federal do Amapá (UNIFAP)
repository.mail.fl_str_mv ||juliosa@unifap.br
_version_ 1788166063471460352

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