Phytochemical prospection, total phenol content and anti-radical activity of leaf extract and fractions of Annona sylvatica (Annonaceae)

Detalhes bibliográficos
Autor(a) principal: Trindade, Tamara Mendes Leite Silva
Data de Publicação: 2021
Outros Autores: Carvalho Junior, Almir Ribeiro de, Ferreira, Rafaela Oliveira
Tipo de documento: Artigo
Idioma: por
Título da fonte: Research, Society and Development
Texto Completo: https://rsdjournal.org/index.php/rsd/article/view/22811
Resumo: The consumption of medicinal plants has shown considerable growth in several countries. Annona sylvatica, commonly known as araticum-do-mato, is a fruit used in folk medicine to lower blood pressure. The objective of this work was to investigate the phytochemical profile and evaluate the antioxidant activity of A. sylvatica leaf extracts and fractions. The ethanol extract was obtained by maceration and submitted to liquid-liquid partition to obtain fractions in hexane, chloroform and ethyl acetate. The main classes of secondary metabolites were detected by phytochemical screening, the content of total phenols (TF) and the antioxidant activity were quantified by the Folin-Dennis tests and the DPPH radical scavenging activity assay, respectively. Using the Pearson coefficient, the total phenol contents were correlated with the EC50 values observed in the DPPH assay. Phytochemical screening detected the presence of flavonoids, saponins, alkaloids and triterpenes/steroids. The AcOET fraction showed the highest content of total phenols and the highest activity in the DPPH assay, with values of 84.0±4.4 mg GAE. g-1 and EC50 9.28 µg. mL-1, respectively. There was a strong negative correlation between TF content and EC50 values, suggesting that part of the antioxidant activity observed is related to TF content. The results suggest that araticum-do-mato is an important source of natural antioxidants.
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spelling Phytochemical prospection, total phenol content and anti-radical activity of leaf extract and fractions of Annona sylvatica (Annonaceae)Prospección fitoquímica, contenido total de fenoles y actividad antirradicales del extracto de hoja y fracciones de Annona sylvatica (Annonaceae)Prospecção fitoquímica, teor de fenóis totais e atividade antiradicalar de extrato e frações de folhas de Annona sylvatica (Annonaceae)Araticum-do-matoDPPHFitoquímicaFlavonoidesAntioxidantes.Araticum-do-matoPhytochemistryFlavonoidsDPPHAntioxidants.Araticum-do-matoFitoquímicaFlavonoidesDPPHAntioxidantes.The consumption of medicinal plants has shown considerable growth in several countries. Annona sylvatica, commonly known as araticum-do-mato, is a fruit used in folk medicine to lower blood pressure. The objective of this work was to investigate the phytochemical profile and evaluate the antioxidant activity of A. sylvatica leaf extracts and fractions. The ethanol extract was obtained by maceration and submitted to liquid-liquid partition to obtain fractions in hexane, chloroform and ethyl acetate. The main classes of secondary metabolites were detected by phytochemical screening, the content of total phenols (TF) and the antioxidant activity were quantified by the Folin-Dennis tests and the DPPH radical scavenging activity assay, respectively. Using the Pearson coefficient, the total phenol contents were correlated with the EC50 values observed in the DPPH assay. Phytochemical screening detected the presence of flavonoids, saponins, alkaloids and triterpenes/steroids. The AcOET fraction showed the highest content of total phenols and the highest activity in the DPPH assay, with values of 84.0±4.4 mg GAE. g-1 and EC50 9.28 µg. mL-1, respectively. There was a strong negative correlation between TF content and EC50 values, suggesting that part of the antioxidant activity observed is related to TF content. The results suggest that araticum-do-mato is an important source of natural antioxidants.El consumo de plantas medicinales ha mostrado un crecimiento considerable en varios países. Annona sylvatica, comúnmente conocida como araticum-do-mato, es una fruta que se usa en la medicina popular para reducir la presión arterial. El objetivo de este trabajo fue investigar el perfil fitoquímico y evaluar la actividad antioxidante de extractos y fracciones de hojas de A. sylvatica. El extracto etanólico se obtuvo por maceración y se sometió a reparto líquido-líquido para obtener fracciones en hexano, cloroformo y acetato de etilo. Las principales clases de metabolitos secundarios se detectaron mediante cribado fitoquímico, el contenido de fenoles totales (TF) y la actividad antioxidante se cuantificaron mediante las pruebas de Folin-Dennis y el ensayo de actividad captadora de radicales DPPH, respectivamente. Usando el coeficiente de Pearson, el contenido total de fenol se correlacionó con los valores de CE50 observados en el ensayo DPPH. El cribado fitoquímico detectó la presencia de flavonoides, saponinas, alcaloides y triterpenos/esteroides. La fracción AcOET mostró el mayor contenido de fenoles totales y la mayor actividad en el ensayo DPPH, con valores de 84,0 ± 4,4 mg EAG. g-1 y EC50 9,28 µg. mL-1, respectivamente. Se observó una fuerte correlación negativa entre el contenido de TF y los valores de CE50, lo que sugiere que parte de la actividad antioxidante observada está relacionada con el contenido de TF. Los resultados sugieren que araticum-do-mato es una fuente importante de antioxidantes naturales.O consumo de plantas medicinais tem apresentado um crescimento considerável em diversos países. Annona sylvatica, conhecida vulgarmente como araticum-do-mato, é uma frutífera empregada na medicina popular para baixar a pressão arterial. O objetivo deste trabalho foi investigar o perfil fitoquímico e avaliar a atividade antioxidante de extrato e frações de folhas de A. sylvatica. O extrato em etanol foi obtido por maceração e submetido à partição líquido-líquido para obtenção das frações em hexano, clorofórmio e acetato de etila. As principais classes de metabólitos secundários foram detectadas por prospecção fitoquímica, o teor de fenóis totais (FT) e a atividade antioxidante foram quantificados pelos testes de Folin-Dennis e ensaio da atividade sequestradora do radical DPPH, respectivamente. Através do coeficiente de Pearson, os teores de fenóis totais foram correlacionados com os valores de CE50 observados no ensaio do DPPH. A prospecção fitoquímica detectou a presença de flavonoides, saponinas, alcaloides e triterpenos/esteroides. A fração AcOET apresentou o maior teor de fenóis totais e maior atividade no ensaio do DPPH, com os valores de 84,0±4,4 mg EAG. g-1 e CE50 9,28 µg. mL-1, respectivamente. Observou-se uma forte correlação negativa entre o teor de FT e os valores de CE50, sugerindo que parte da atividade antioxidante observada está relacionada ao teor de FT. Os resultados sugerem que a araticum-do-mato é uma importante fonte de antioxidantes naturais.Research, Society and Development2021-11-27info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://rsdjournal.org/index.php/rsd/article/view/2281110.33448/rsd-v10i15.22811Research, Society and Development; Vol. 10 No. 15; e340101522811Research, Society and Development; Vol. 10 Núm. 15; e340101522811Research, Society and Development; v. 10 n. 15; e3401015228112525-3409reponame:Research, Society and Developmentinstname:Universidade Federal de Itajubá (UNIFEI)instacron:UNIFEIporhttps://rsdjournal.org/index.php/rsd/article/view/22811/20366Copyright (c) 2021 Tamara Mendes Leite Silva Trindade; Almir Ribeiro de Carvalho Junior; Rafaela Oliveira Ferreirahttps://creativecommons.org/licenses/by/4.0info:eu-repo/semantics/openAccessTrindade, Tamara Mendes Leite SilvaCarvalho Junior, Almir Ribeiro deFerreira, Rafaela Oliveira 2021-12-06T10:13:53Zoai:ojs.pkp.sfu.ca:article/22811Revistahttps://rsdjournal.org/index.php/rsd/indexPUBhttps://rsdjournal.org/index.php/rsd/oairsd.articles@gmail.com2525-34092525-3409opendoar:2024-01-17T09:41:52.076837Research, Society and Development - Universidade Federal de Itajubá (UNIFEI)false
dc.title.none.fl_str_mv Phytochemical prospection, total phenol content and anti-radical activity of leaf extract and fractions of Annona sylvatica (Annonaceae)
Prospección fitoquímica, contenido total de fenoles y actividad antirradicales del extracto de hoja y fracciones de Annona sylvatica (Annonaceae)
Prospecção fitoquímica, teor de fenóis totais e atividade antiradicalar de extrato e frações de folhas de Annona sylvatica (Annonaceae)
title Phytochemical prospection, total phenol content and anti-radical activity of leaf extract and fractions of Annona sylvatica (Annonaceae)
spellingShingle Phytochemical prospection, total phenol content and anti-radical activity of leaf extract and fractions of Annona sylvatica (Annonaceae)
Trindade, Tamara Mendes Leite Silva
Araticum-do-mato
DPPH
Fitoquímica
Flavonoides
Antioxidantes.
Araticum-do-mato
Phytochemistry
Flavonoids
DPPH
Antioxidants.
Araticum-do-mato
Fitoquímica
Flavonoides
DPPH
Antioxidantes.
title_short Phytochemical prospection, total phenol content and anti-radical activity of leaf extract and fractions of Annona sylvatica (Annonaceae)
title_full Phytochemical prospection, total phenol content and anti-radical activity of leaf extract and fractions of Annona sylvatica (Annonaceae)
title_fullStr Phytochemical prospection, total phenol content and anti-radical activity of leaf extract and fractions of Annona sylvatica (Annonaceae)
title_full_unstemmed Phytochemical prospection, total phenol content and anti-radical activity of leaf extract and fractions of Annona sylvatica (Annonaceae)
title_sort Phytochemical prospection, total phenol content and anti-radical activity of leaf extract and fractions of Annona sylvatica (Annonaceae)
author Trindade, Tamara Mendes Leite Silva
author_facet Trindade, Tamara Mendes Leite Silva
Carvalho Junior, Almir Ribeiro de
Ferreira, Rafaela Oliveira
author_role author
author2 Carvalho Junior, Almir Ribeiro de
Ferreira, Rafaela Oliveira
author2_role author
author
dc.contributor.author.fl_str_mv Trindade, Tamara Mendes Leite Silva
Carvalho Junior, Almir Ribeiro de
Ferreira, Rafaela Oliveira
dc.subject.por.fl_str_mv Araticum-do-mato
DPPH
Fitoquímica
Flavonoides
Antioxidantes.
Araticum-do-mato
Phytochemistry
Flavonoids
DPPH
Antioxidants.
Araticum-do-mato
Fitoquímica
Flavonoides
DPPH
Antioxidantes.
topic Araticum-do-mato
DPPH
Fitoquímica
Flavonoides
Antioxidantes.
Araticum-do-mato
Phytochemistry
Flavonoids
DPPH
Antioxidants.
Araticum-do-mato
Fitoquímica
Flavonoides
DPPH
Antioxidantes.
description The consumption of medicinal plants has shown considerable growth in several countries. Annona sylvatica, commonly known as araticum-do-mato, is a fruit used in folk medicine to lower blood pressure. The objective of this work was to investigate the phytochemical profile and evaluate the antioxidant activity of A. sylvatica leaf extracts and fractions. The ethanol extract was obtained by maceration and submitted to liquid-liquid partition to obtain fractions in hexane, chloroform and ethyl acetate. The main classes of secondary metabolites were detected by phytochemical screening, the content of total phenols (TF) and the antioxidant activity were quantified by the Folin-Dennis tests and the DPPH radical scavenging activity assay, respectively. Using the Pearson coefficient, the total phenol contents were correlated with the EC50 values observed in the DPPH assay. Phytochemical screening detected the presence of flavonoids, saponins, alkaloids and triterpenes/steroids. The AcOET fraction showed the highest content of total phenols and the highest activity in the DPPH assay, with values of 84.0±4.4 mg GAE. g-1 and EC50 9.28 µg. mL-1, respectively. There was a strong negative correlation between TF content and EC50 values, suggesting that part of the antioxidant activity observed is related to TF content. The results suggest that araticum-do-mato is an important source of natural antioxidants.
publishDate 2021
dc.date.none.fl_str_mv 2021-11-27
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv https://rsdjournal.org/index.php/rsd/article/view/22811
10.33448/rsd-v10i15.22811
url https://rsdjournal.org/index.php/rsd/article/view/22811
identifier_str_mv 10.33448/rsd-v10i15.22811
dc.language.iso.fl_str_mv por
language por
dc.relation.none.fl_str_mv https://rsdjournal.org/index.php/rsd/article/view/22811/20366
dc.rights.driver.fl_str_mv https://creativecommons.org/licenses/by/4.0
info:eu-repo/semantics/openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by/4.0
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Research, Society and Development
publisher.none.fl_str_mv Research, Society and Development
dc.source.none.fl_str_mv Research, Society and Development; Vol. 10 No. 15; e340101522811
Research, Society and Development; Vol. 10 Núm. 15; e340101522811
Research, Society and Development; v. 10 n. 15; e340101522811
2525-3409
reponame:Research, Society and Development
instname:Universidade Federal de Itajubá (UNIFEI)
instacron:UNIFEI
instname_str Universidade Federal de Itajubá (UNIFEI)
instacron_str UNIFEI
institution UNIFEI
reponame_str Research, Society and Development
collection Research, Society and Development
repository.name.fl_str_mv Research, Society and Development - Universidade Federal de Itajubá (UNIFEI)
repository.mail.fl_str_mv rsd.articles@gmail.com
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