Isolation and identification of Brucella suis biovar 1 in pork samples (sus scrofa) from the municipality of Cachoeira do Arari, State of Para-Brazil.

• Autor(a) principal: Ampuero, Naiana Fleury da Fonseca
• Data de Publicação: 2022
• Outros Autores: Silva, Sandro Patroca da, Pereira, Washington Luiz Assunção, Benigno, Raimundo Nonato Moraes, Carneiro, Adriana Ribeiro, Aragão, Andressa de Oliveira, Casseb, Lívia Medeiros Neves, Nunes, Bruno Tardelli Diniz, Casseb, Alexandre do Rosário
• Tipo de documento: Artigo
• Idioma: por
• Título da fonte: Research, Society and Development
• Texto Completo: https://rsdjournal.org/index.php/rsd/article/view/26351

This work aimed to study the microorganisms present in lesions found in swine from the municipality of Cachoeira do Arari (Ilha do Marajó), sold at the Ver-o-Peso fair in Belém do Pará; were carried out the isolation, nucleotide sequencing and phylogenetic inference of the collected material. There was positive growth on brain-heart broth and blood agar and no growth on MacConkey agar. In the gram staining, grouped gram negative cocci were observed, and in the Modified Ziehl-Neelsen staining, grouped cocci stained in red were observed. These growth characteristics, as well as the observed microscopic results, were suggestive and similar to bacteria belonging to the genus Brucella sp. After a result suggestive of Brucella spp, the sample was sent for DNA extraction, nucleotide sequencing. Brucella suis biovar 1 was identified; with subsequent assembly of the phylogenetic tree. In addition to the sanitary issue, clandestine slaughter also involves the environmental issue, as it is usually carried out in places close to rivers or streams to facilitate the capture of water. The identification of bacteria with morphological characteristics similar to the Brucella genus of the swine abscess observed in this study confirms that the animal had swine brucellosis and that clandestine slaughterhouses can be important means of infection and dissemination of these agents, if not have verification that guarantee the use sanitary surveillance measures of quality that come prevent this type of practice and the sale of the product for human consumption.