Morphometry, stereology and liver biochemistry tests in infant albino rats submitted to residual ingestion of pregabalin in breast milk
Autor(a) principal: | |
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Data de Publicação: | 2021 |
Outros Autores: | , , , , , , |
Tipo de documento: | Artigo |
Idioma: | por |
Título da fonte: | Research, Society and Development |
Texto Completo: | https://rsdjournal.org/index.php/rsd/article/view/24117 |
Resumo: | This study has evaluated the effects of pregabalin on the liver of lactating rats, derived from the offspring of mothers who received the drug during lactation. Twenty-four albino lactating rats with 14 and 21 days of life were used. They were divided into control (CG) and pregabalin (PG) groups, and these were subdivided according to the age at euthanasia (CG14, CG21, PG14, PG21). The lactating mothers of the treatment group received, orally, the psychotropic drug pregabalin during the 21 days of nursing. The ones of the control group received only saline solution. On the date of euthanasia, morphometric and stereological values were measured. The samples were fixed and liver sections were processed. Area and diameter of hepatocyte nuclei; area of hepatocytes; area and diameter of bile ducts; and the number of hepatocytes per area; as well as volume densities of hepatocytes, and interstitial tissue were obtained. The results were compared by the Mann-Whitney U statistical test, for variables with non-parametric distribution, and Student's t-test for parametric variables. Animals in the treatment group showed an increase in biometric variables at both ages studied. There was an increase in the area of hepatocytes at 14 and 21 days of life, and an increase in the diameter of the hepatocyte nuclei of euthanized animals at 21 days of age. There was an increase in the volume of liver tissues in the 14-day-old group. Liver enzymes were unchanged. Pregabalin promoted hepatocyte hypertrophy (Megalocytosis) without compromising the functional activity of the cells. |
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Morphometry, stereology and liver biochemistry tests in infant albino rats submitted to residual ingestion of pregabalin in breast milk Morfometría, estereología y bioquímica hepática en ratas albinas lactantes sometidas a ingestión residual de pregabalina en leche materna Morfometria, estereologia e bioquímica hepática de ratos albinos lactentes submetidos à ingestão residual da pregabalina no leite materno BiometriaFígadoPsicofármacoPregabalina.EstereologíHígadoPsicofármacoPregabalina.LiverPsychopharmaceuticalPregabalinStereology.This study has evaluated the effects of pregabalin on the liver of lactating rats, derived from the offspring of mothers who received the drug during lactation. Twenty-four albino lactating rats with 14 and 21 days of life were used. They were divided into control (CG) and pregabalin (PG) groups, and these were subdivided according to the age at euthanasia (CG14, CG21, PG14, PG21). The lactating mothers of the treatment group received, orally, the psychotropic drug pregabalin during the 21 days of nursing. The ones of the control group received only saline solution. On the date of euthanasia, morphometric and stereological values were measured. The samples were fixed and liver sections were processed. Area and diameter of hepatocyte nuclei; area of hepatocytes; area and diameter of bile ducts; and the number of hepatocytes per area; as well as volume densities of hepatocytes, and interstitial tissue were obtained. The results were compared by the Mann-Whitney U statistical test, for variables with non-parametric distribution, and Student's t-test for parametric variables. Animals in the treatment group showed an increase in biometric variables at both ages studied. There was an increase in the area of hepatocytes at 14 and 21 days of life, and an increase in the diameter of the hepatocyte nuclei of euthanized animals at 21 days of age. There was an increase in the volume of liver tissues in the 14-day-old group. Liver enzymes were unchanged. Pregabalin promoted hepatocyte hypertrophy (Megalocytosis) without compromising the functional activity of the cells.El estudio evaluó los efectos de la pregabalina en el hígado de ratas lactantes de la descendencia de hembras que recibieron el fármaco durante la lactancia. Se utilizaron veinticuatro ratas lactantes albinas con 14 y 21 días de vida. Los animales se dividieron en grupos de control (CG) y pregabalina (GP), y estos se subdividieron según la edad de eutanasia (GC14, GC21, GP14, GP21). Las madres lactantes de los grupos tratados recibieron, por vía oral, el fármaco psicotrópico pregabalina durante los 21 días de lactancia. Las cerdas de los grupos de control recibieron solo solución salina. En la fecha de la eutanasia se midieron los valores morfométricos y estereológicos. Las muestras se fijaron y se procesaron secciones de hígado. Se obtuvieron el área y diámetro de los núcleos de los hepatocitos, el área de los hepatocitos, el área y el diámetro de los conductos biliares y el número de hepatocitos por área, así como las densidades de volumen de los hepatocitos y el tejido intersticial. Los resultados se compararon mediante la prueba estadística “U” de Mann-Whitney, para variables con distribución no paramétrica, y la prueba “t” de Student para variables paramétricas. Los animales tratados mostraron un aumento de las variables biométricas en las dos edades estudiadas. Hubo un aumento en el área de los hepatocitos a los 14 y 21 días de vida, y un aumento en el diámetro de los núcleos de los hepatocitos de los animales sacrificados a los 21 días de edad. Hubo un aumento en el volumen de tejidos hepáticos en el grupo de 14 días. Las enzimas hepáticas no cambiaron. La pregabalina promovió la hipertrofia de los hepatocitos (megalocitosis) sin comprometer la actividad funcional de las células.O estudo avaliou os efeitos da pregabalina sobre o fígado de ratos lactentes, oriundos da prole de fêmeas que receberam a droga durante a lactação. Foram utilizados 24 ratos albinos lactentes com 14 e 21 dias de vida. Os animais foram divididos em grupos controle (GC) e pregabalina (GP), e estes, subdivididos, de acordo com a idade de eutanásia (GC14, GC21, GP14, GP21). As matrizes lactantes dos grupos tratados receberam por via oral, o psicofármaco pregabalina durante os 21 dias de amamentação. As matrizes dos grupos controles receberam apenas solução fisiológica. Na data da eutanásia, valores morfométricos e estereológicos foram aferidos. As amostras foram fixadas e secções de fígado foram processadas. Área e diâmetros dos núcleos de hepatócitos, área dos hepatócitos, área e diâmetro de ductos biliares e número de hepatócitos por área além de densidades de volume dos hepatócitos e do tecido intersticial foram obtidos. Os resultados foram confrontados pelo teste estatístico “U” de Mann-Whitney, para variáveis com distribuição não paramétrica, e teste “t” de Student para variáveis paramétricas. Os animais tratados apresentaram aumento de variáveis biométricas em ambas as idades estudadas. Houve aumento da área dos hepatócitos aos 14 e 21 dias de vida, e aumento do diâmetro dos núcleos dos hepatócitos dos animais eutanasiados aos 21 dias de idade. Verificou-se aumento do volume dos tecidos hepáticos no grupo de 14 dias de vida. As enzimas hepáticas não sofreram alterações. A pregabalina promoveu hipertrofia de hepatócitos (Megalocitose) sem comprometimento da atividade funcional das células.Research, Society and Development2021-12-17info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://rsdjournal.org/index.php/rsd/article/view/2411710.33448/rsd-v10i16.24117Research, Society and Development; Vol. 10 No. 16; e491101624117Research, Society and Development; Vol. 10 Núm. 16; e491101624117Research, Society and Development; v. 10 n. 16; e4911016241172525-3409reponame:Research, Society and Developmentinstname:Universidade Federal de Itajubá (UNIFEI)instacron:UNIFEIporhttps://rsdjournal.org/index.php/rsd/article/view/24117/21143Copyright (c) 2021 Maria Eduarda Camargo Caldeira; Edson Vinícius Leite Veloso; José Emanuel de Souza Sales; Raul Anderson de Souza Machado; Paula Páglia Braga Araújo; Ruthyanna Camila Medeiros da Silva; Danilo José Ayres de Menezes; Otávio Brilhante de Sousahttps://creativecommons.org/licenses/by/4.0info:eu-repo/semantics/openAccessCaldeira, Maria Eduarda CamargoVeloso, Edson Vinícius LeiteSales, José Emanuel de SouzaMachado, Raul Anderson de Souza Araújo, Paula Páglia Braga Silva, Ruthyanna Camila Medeiros da Menezes, Danilo José Ayres de Sousa, Otávio Brilhante de 2021-12-20T11:03:07Zoai:ojs.pkp.sfu.ca:article/24117Revistahttps://rsdjournal.org/index.php/rsd/indexPUBhttps://rsdjournal.org/index.php/rsd/oairsd.articles@gmail.com2525-34092525-3409opendoar:2024-01-17T09:42:46.625454Research, Society and Development - Universidade Federal de Itajubá (UNIFEI)false |
dc.title.none.fl_str_mv |
Morphometry, stereology and liver biochemistry tests in infant albino rats submitted to residual ingestion of pregabalin in breast milk Morfometría, estereología y bioquímica hepática en ratas albinas lactantes sometidas a ingestión residual de pregabalina en leche materna Morfometria, estereologia e bioquímica hepática de ratos albinos lactentes submetidos à ingestão residual da pregabalina no leite materno |
title |
Morphometry, stereology and liver biochemistry tests in infant albino rats submitted to residual ingestion of pregabalin in breast milk |
spellingShingle |
Morphometry, stereology and liver biochemistry tests in infant albino rats submitted to residual ingestion of pregabalin in breast milk Caldeira, Maria Eduarda Camargo Biometria Fígado Psicofármaco Pregabalina. Estereologí Hígado Psicofármaco Pregabalina. Liver Psychopharmaceutical Pregabalin Stereology. |
title_short |
Morphometry, stereology and liver biochemistry tests in infant albino rats submitted to residual ingestion of pregabalin in breast milk |
title_full |
Morphometry, stereology and liver biochemistry tests in infant albino rats submitted to residual ingestion of pregabalin in breast milk |
title_fullStr |
Morphometry, stereology and liver biochemistry tests in infant albino rats submitted to residual ingestion of pregabalin in breast milk |
title_full_unstemmed |
Morphometry, stereology and liver biochemistry tests in infant albino rats submitted to residual ingestion of pregabalin in breast milk |
title_sort |
Morphometry, stereology and liver biochemistry tests in infant albino rats submitted to residual ingestion of pregabalin in breast milk |
author |
Caldeira, Maria Eduarda Camargo |
author_facet |
Caldeira, Maria Eduarda Camargo Veloso, Edson Vinícius Leite Sales, José Emanuel de Souza Machado, Raul Anderson de Souza Araújo, Paula Páglia Braga Silva, Ruthyanna Camila Medeiros da Menezes, Danilo José Ayres de Sousa, Otávio Brilhante de |
author_role |
author |
author2 |
Veloso, Edson Vinícius Leite Sales, José Emanuel de Souza Machado, Raul Anderson de Souza Araújo, Paula Páglia Braga Silva, Ruthyanna Camila Medeiros da Menezes, Danilo José Ayres de Sousa, Otávio Brilhante de |
author2_role |
author author author author author author author |
dc.contributor.author.fl_str_mv |
Caldeira, Maria Eduarda Camargo Veloso, Edson Vinícius Leite Sales, José Emanuel de Souza Machado, Raul Anderson de Souza Araújo, Paula Páglia Braga Silva, Ruthyanna Camila Medeiros da Menezes, Danilo José Ayres de Sousa, Otávio Brilhante de |
dc.subject.por.fl_str_mv |
Biometria Fígado Psicofármaco Pregabalina. Estereologí Hígado Psicofármaco Pregabalina. Liver Psychopharmaceutical Pregabalin Stereology. |
topic |
Biometria Fígado Psicofármaco Pregabalina. Estereologí Hígado Psicofármaco Pregabalina. Liver Psychopharmaceutical Pregabalin Stereology. |
description |
This study has evaluated the effects of pregabalin on the liver of lactating rats, derived from the offspring of mothers who received the drug during lactation. Twenty-four albino lactating rats with 14 and 21 days of life were used. They were divided into control (CG) and pregabalin (PG) groups, and these were subdivided according to the age at euthanasia (CG14, CG21, PG14, PG21). The lactating mothers of the treatment group received, orally, the psychotropic drug pregabalin during the 21 days of nursing. The ones of the control group received only saline solution. On the date of euthanasia, morphometric and stereological values were measured. The samples were fixed and liver sections were processed. Area and diameter of hepatocyte nuclei; area of hepatocytes; area and diameter of bile ducts; and the number of hepatocytes per area; as well as volume densities of hepatocytes, and interstitial tissue were obtained. The results were compared by the Mann-Whitney U statistical test, for variables with non-parametric distribution, and Student's t-test for parametric variables. Animals in the treatment group showed an increase in biometric variables at both ages studied. There was an increase in the area of hepatocytes at 14 and 21 days of life, and an increase in the diameter of the hepatocyte nuclei of euthanized animals at 21 days of age. There was an increase in the volume of liver tissues in the 14-day-old group. Liver enzymes were unchanged. Pregabalin promoted hepatocyte hypertrophy (Megalocytosis) without compromising the functional activity of the cells. |
publishDate |
2021 |
dc.date.none.fl_str_mv |
2021-12-17 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
https://rsdjournal.org/index.php/rsd/article/view/24117 10.33448/rsd-v10i16.24117 |
url |
https://rsdjournal.org/index.php/rsd/article/view/24117 |
identifier_str_mv |
10.33448/rsd-v10i16.24117 |
dc.language.iso.fl_str_mv |
por |
language |
por |
dc.relation.none.fl_str_mv |
https://rsdjournal.org/index.php/rsd/article/view/24117/21143 |
dc.rights.driver.fl_str_mv |
https://creativecommons.org/licenses/by/4.0 info:eu-repo/semantics/openAccess |
rights_invalid_str_mv |
https://creativecommons.org/licenses/by/4.0 |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Research, Society and Development |
publisher.none.fl_str_mv |
Research, Society and Development |
dc.source.none.fl_str_mv |
Research, Society and Development; Vol. 10 No. 16; e491101624117 Research, Society and Development; Vol. 10 Núm. 16; e491101624117 Research, Society and Development; v. 10 n. 16; e491101624117 2525-3409 reponame:Research, Society and Development instname:Universidade Federal de Itajubá (UNIFEI) instacron:UNIFEI |
instname_str |
Universidade Federal de Itajubá (UNIFEI) |
instacron_str |
UNIFEI |
institution |
UNIFEI |
reponame_str |
Research, Society and Development |
collection |
Research, Society and Development |
repository.name.fl_str_mv |
Research, Society and Development - Universidade Federal de Itajubá (UNIFEI) |
repository.mail.fl_str_mv |
rsd.articles@gmail.com |
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1797052699414364160 |