Antifungal activity, antibiofilm and synergic effect of diallyl disulfide and diallyl trisulfide against Candida albicans

Detalhes bibliográficos
Autor(a) principal: Pereira, Rafael
Data de Publicação: 2022
Outros Autores: Prado, Guilherme Mendes, Fontenelle, Raquel Oliveira dos Santos, Barbosa, Francisco César Barroso, Santos, Hélcio Silva dos, Marinho, Emmanuel Silva, Marinho, Márcia Machado, Morais, Selene Maia de
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Research, Society and Development
Texto Completo: https://rsdjournal.org/index.php/rsd/article/view/27538
Resumo: The objective of the present study was to determine the antifungal activity, antibiofilm and synergic effect of the compounds diallyl disulfide and diallyl trisulfide in combination with antifungal agents against clinical isolates of Candida albicans, including also a computational study. Antimicrobial sensitivity tests were performed using the broth microdilution method to determine the minimum inhibitory concentration against C. albicans strains and modulatory activity using the checkerboard technique. The biofilm formation was evaluated by biomass quantification using the violet crystal staining method. For the study of molecular docking computer simulations. The constituents showed relevant antifungal activity against strains of C. albicans. In the modulatory activity assay, demonstrated a synergistic interaction with fluconazole and amphotericin B, with an increase in its antifungal action. The diallyl disulfide, diallyl trisulfide and fluconazole ligands formed complexes with ALS3 enzyme. Then, both compounds were considered promising products for the development of new drugs to prevent candidiasis.
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spelling Antifungal activity, antibiofilm and synergic effect of diallyl disulfide and diallyl trisulfide against Candida albicansActividad antifúngica, antibiopelícula y efecto sinérgico de disulfuro de dialilo y trisulfuro de dialilo frente a Candida albicansAtividade antifúngica, antibiofilme e efeito sinérgico do dissulfeto de dialila e trissulfeto de dialila frente a espécie Candida albicansCandidiasisAntifúngicoBiopelículaAllium sativumIn silico.CandidíaseAntifúngicoBiofilmeAllium sativumIn sílico.CandidiasisAntifungalBiofilmAllium sativumIn silico.The objective of the present study was to determine the antifungal activity, antibiofilm and synergic effect of the compounds diallyl disulfide and diallyl trisulfide in combination with antifungal agents against clinical isolates of Candida albicans, including also a computational study. Antimicrobial sensitivity tests were performed using the broth microdilution method to determine the minimum inhibitory concentration against C. albicans strains and modulatory activity using the checkerboard technique. The biofilm formation was evaluated by biomass quantification using the violet crystal staining method. For the study of molecular docking computer simulations. The constituents showed relevant antifungal activity against strains of C. albicans. In the modulatory activity assay, demonstrated a synergistic interaction with fluconazole and amphotericin B, with an increase in its antifungal action. The diallyl disulfide, diallyl trisulfide and fluconazole ligands formed complexes with ALS3 enzyme. Then, both compounds were considered promising products for the development of new drugs to prevent candidiasis.El objetivo del presente estudio fue determinar la actividad antifúngica, antibiopelícula y efecto sinérgico de los compuestos disulfuro de dialilo y trisulfuro de dialilo en combinación con agentes antifúngicos contra aislados clínicos de Candida albicans, incluyendo además un estudio computacional. Se realizaron pruebas de sensibilidad antimicrobiana mediante el método de microdilución en caldo para determinar la concentración inhibitoria mínima frente a las cepas de C. albicans y la actividad moduladora mediante la técnica del tablero de ajedrez. La formación de biopelículas se evaluó mediante la cuantificación de biomasa utilizando el método de tinción de cristales violetas. Para el estudio de simulaciones informáticas de acoplamiento molecular. Los constituyentes mostraron una actividad antifúngica relevante contra cepas de C. albicans. En el ensayo de actividad moduladora, demostró una interacción sinérgica con fluconazol y anfotericina B, con un aumento de su acción antifúngica. Los ligandos disulfuro de dialilo, trisulfuro de dialilo y fluconazol formaron complejos con la enzima ALS3. Entonces, ambos compuestos se consideraron productos prometedores para el desarrollo de nuevos fármacos para prevenir la candidiasis.O objetivo do presente estudo foi determinar a atividade antifúngica, antibiofilme e efeito sinérgico dos compostos dissulfeto de dialila e trissulfeto de dialila em combinação com agentes antifúngicos contra isolados clínicos de Candida albicans, incluindo também um estudo computacional. Testes de sensibilidade antimicrobiana foram realizados pelo método de microdiluição em caldo para determinar a concentração inibitória mínima contra cepas de C. albicans e a atividade modulatória usando a técnica checkerboard. A formação de biofilme foi avaliada pela quantificação da biomassa pelo método de coloração por cristal violeta. Para o estudo de simulações computacionais de encaixe molecular. Os constituintes apresentaram atividade antifúngica relevante contra cepas de C. albicans. No ensaio de atividade modulatória, demonstrou interação sinérgica com fluconazol e anfotericina B, com aumento de sua ação antifúngica. Os ligantes dissulfeto de dialila, trissulfeto de dialila e fluconazol formaram complexos com a enzima ALS3. Então, ambos os compostos foram considerados produtos promissores para o desenvolvimento de novos medicamentos para prevenir a candidíase.Research, Society and Development2022-03-22info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://rsdjournal.org/index.php/rsd/article/view/2753810.33448/rsd-v11i4.27538Research, Society and Development; Vol. 11 No. 4; e42111427538Research, Society and Development; Vol. 11 Núm. 4; e42111427538Research, Society and Development; v. 11 n. 4; e421114275382525-3409reponame:Research, Society and Developmentinstname:Universidade Federal de Itajubá (UNIFEI)instacron:UNIFEIenghttps://rsdjournal.org/index.php/rsd/article/view/27538/24056Copyright (c) 2022 Rafael Pereira; Guilherme Mendes Prado; Raquel Oliveira dos Santos Fontenelle; Francisco César Barroso Barbosa; Hélcio Silva dos Santos; Emmanuel Silva Marinho; Márcia Machado Marinho; Selene Maia de Moraishttps://creativecommons.org/licenses/by/4.0info:eu-repo/semantics/openAccessPereira, RafaelPrado, Guilherme Mendes Fontenelle, Raquel Oliveira dos Santos Barbosa, Francisco César Barroso Santos, Hélcio Silva dos Marinho, Emmanuel Silva Marinho, Márcia Machado Morais, Selene Maia de 2022-03-27T17:17:09Zoai:ojs.pkp.sfu.ca:article/27538Revistahttps://rsdjournal.org/index.php/rsd/indexPUBhttps://rsdjournal.org/index.php/rsd/oairsd.articles@gmail.com2525-34092525-3409opendoar:2024-01-17T09:45:14.983043Research, Society and Development - Universidade Federal de Itajubá (UNIFEI)false
dc.title.none.fl_str_mv Antifungal activity, antibiofilm and synergic effect of diallyl disulfide and diallyl trisulfide against Candida albicans
Actividad antifúngica, antibiopelícula y efecto sinérgico de disulfuro de dialilo y trisulfuro de dialilo frente a Candida albicans
Atividade antifúngica, antibiofilme e efeito sinérgico do dissulfeto de dialila e trissulfeto de dialila frente a espécie Candida albicans
title Antifungal activity, antibiofilm and synergic effect of diallyl disulfide and diallyl trisulfide against Candida albicans
spellingShingle Antifungal activity, antibiofilm and synergic effect of diallyl disulfide and diallyl trisulfide against Candida albicans
Pereira, Rafael
Candidiasis
Antifúngico
Biopelícula
Allium sativum
In silico.
Candidíase
Antifúngico
Biofilme
Allium sativum
In sílico.
Candidiasis
Antifungal
Biofilm
Allium sativum
In silico.
title_short Antifungal activity, antibiofilm and synergic effect of diallyl disulfide and diallyl trisulfide against Candida albicans
title_full Antifungal activity, antibiofilm and synergic effect of diallyl disulfide and diallyl trisulfide against Candida albicans
title_fullStr Antifungal activity, antibiofilm and synergic effect of diallyl disulfide and diallyl trisulfide against Candida albicans
title_full_unstemmed Antifungal activity, antibiofilm and synergic effect of diallyl disulfide and diallyl trisulfide against Candida albicans
title_sort Antifungal activity, antibiofilm and synergic effect of diallyl disulfide and diallyl trisulfide against Candida albicans
author Pereira, Rafael
author_facet Pereira, Rafael
Prado, Guilherme Mendes
Fontenelle, Raquel Oliveira dos Santos
Barbosa, Francisco César Barroso
Santos, Hélcio Silva dos
Marinho, Emmanuel Silva
Marinho, Márcia Machado
Morais, Selene Maia de
author_role author
author2 Prado, Guilherme Mendes
Fontenelle, Raquel Oliveira dos Santos
Barbosa, Francisco César Barroso
Santos, Hélcio Silva dos
Marinho, Emmanuel Silva
Marinho, Márcia Machado
Morais, Selene Maia de
author2_role author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv Pereira, Rafael
Prado, Guilherme Mendes
Fontenelle, Raquel Oliveira dos Santos
Barbosa, Francisco César Barroso
Santos, Hélcio Silva dos
Marinho, Emmanuel Silva
Marinho, Márcia Machado
Morais, Selene Maia de
dc.subject.por.fl_str_mv Candidiasis
Antifúngico
Biopelícula
Allium sativum
In silico.
Candidíase
Antifúngico
Biofilme
Allium sativum
In sílico.
Candidiasis
Antifungal
Biofilm
Allium sativum
In silico.
topic Candidiasis
Antifúngico
Biopelícula
Allium sativum
In silico.
Candidíase
Antifúngico
Biofilme
Allium sativum
In sílico.
Candidiasis
Antifungal
Biofilm
Allium sativum
In silico.
description The objective of the present study was to determine the antifungal activity, antibiofilm and synergic effect of the compounds diallyl disulfide and diallyl trisulfide in combination with antifungal agents against clinical isolates of Candida albicans, including also a computational study. Antimicrobial sensitivity tests were performed using the broth microdilution method to determine the minimum inhibitory concentration against C. albicans strains and modulatory activity using the checkerboard technique. The biofilm formation was evaluated by biomass quantification using the violet crystal staining method. For the study of molecular docking computer simulations. The constituents showed relevant antifungal activity against strains of C. albicans. In the modulatory activity assay, demonstrated a synergistic interaction with fluconazole and amphotericin B, with an increase in its antifungal action. The diallyl disulfide, diallyl trisulfide and fluconazole ligands formed complexes with ALS3 enzyme. Then, both compounds were considered promising products for the development of new drugs to prevent candidiasis.
publishDate 2022
dc.date.none.fl_str_mv 2022-03-22
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv https://rsdjournal.org/index.php/rsd/article/view/27538
10.33448/rsd-v11i4.27538
url https://rsdjournal.org/index.php/rsd/article/view/27538
identifier_str_mv 10.33448/rsd-v11i4.27538
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv https://rsdjournal.org/index.php/rsd/article/view/27538/24056
dc.rights.driver.fl_str_mv https://creativecommons.org/licenses/by/4.0
info:eu-repo/semantics/openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by/4.0
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Research, Society and Development
publisher.none.fl_str_mv Research, Society and Development
dc.source.none.fl_str_mv Research, Society and Development; Vol. 11 No. 4; e42111427538
Research, Society and Development; Vol. 11 Núm. 4; e42111427538
Research, Society and Development; v. 11 n. 4; e42111427538
2525-3409
reponame:Research, Society and Development
instname:Universidade Federal de Itajubá (UNIFEI)
instacron:UNIFEI
instname_str Universidade Federal de Itajubá (UNIFEI)
instacron_str UNIFEI
institution UNIFEI
reponame_str Research, Society and Development
collection Research, Society and Development
repository.name.fl_str_mv Research, Society and Development - Universidade Federal de Itajubá (UNIFEI)
repository.mail.fl_str_mv rsd.articles@gmail.com
_version_ 1797052707753689088

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