Genetic variability of Colletotrichum sublineolum through ISSR markers
Autor(a) principal: | |
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Data de Publicação: | 2021 |
Outros Autores: | , , , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Research, Society and Development |
Texto Completo: | https://rsdjournal.org/index.php/rsd/article/view/12223 |
Resumo: | The occurrence of diseases is a limiting factor in the development of sorghum crop. Among the diseases that causes losses in sorghum production, anthracnose is the main and most severe, mainly by the genetic variability of the pathogen. In this context, the aim of this study was to evaluate the genetic variability of Colletotrichum sublineolum isolates. DNA were extracted from 56 monosporic isolates of C. sublineolum using a DNA extraction kit, and to perform the analysis of genetic diversity of the isolates were used ISSR primers. After amplification, it was determined the polymorphic information content (PIC), allelic frequency, UPGMA and Tocher clustering analyzes and, using software Structure, the genetic structure. According to the descriptive analysis of the genetic variability of C. Sublineolum isolates, primer AP1 presented the higher value of polymorphic information content (PIC). The higher allelic frequency was observed in loci 06, 09, 10, and 24, and the lowest in locus 02. As for the clustering method, it was observed a tendency of grouping C. sublineolum isolates according the geographic origin and, in addition to demonstrating the genetic variability between the C. sublineolum isolates, it was observed the occurrence of introgression among the isolates. |
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Genetic variability of Colletotrichum sublineolum through ISSR markers Variabilidad genética de Colletotrichum sublineolum através de marcadores ISSRVariabilidade genética de Colletotrichum sublineolum através de Marcadores ISSR Sorghum biocolor (L.) MoenchAnthracnoseMolecular markes.Sorghum biocolor (L.) MoenchAntracnosisMarcadores moleculares.Sorghum biocolor (L.) MoenchAntracnoseMarcadores moleculares.The occurrence of diseases is a limiting factor in the development of sorghum crop. Among the diseases that causes losses in sorghum production, anthracnose is the main and most severe, mainly by the genetic variability of the pathogen. In this context, the aim of this study was to evaluate the genetic variability of Colletotrichum sublineolum isolates. DNA were extracted from 56 monosporic isolates of C. sublineolum using a DNA extraction kit, and to perform the analysis of genetic diversity of the isolates were used ISSR primers. After amplification, it was determined the polymorphic information content (PIC), allelic frequency, UPGMA and Tocher clustering analyzes and, using software Structure, the genetic structure. According to the descriptive analysis of the genetic variability of C. Sublineolum isolates, primer AP1 presented the higher value of polymorphic information content (PIC). The higher allelic frequency was observed in loci 06, 09, 10, and 24, and the lowest in locus 02. As for the clustering method, it was observed a tendency of grouping C. sublineolum isolates according the geographic origin and, in addition to demonstrating the genetic variability between the C. sublineolum isolates, it was observed the occurrence of introgression among the isolates.La aparición de enfermedades es un factor limitante en el desarrollo del cultivo de sorgo. Entre las enfermedades que ocasionan pérdidas en la producción de sorgo, la antracnosis es la principal y más grave, principalmente por la variabilidad genética del patógeno. En este contexto, el objetivo de este estudio fue evaluar la variabilidad genética de aislados de Colletotrichum sublineolum. Se extrajo ADN de 56 aislados monospóricos de C. sublineolum utilizando un kit de extracción de ADN, y para realizar el análisis de diversidad genética de los aislados se utilizaron cebadores ISSR. Después de la amplificación, se determinó el contenido de información polimórfica (PIC), la frecuencia alélica, los análisis de agrupamiento de UPGMA y Tocher y, utilizando el software Structure, la estructura genética. Según el análisis descriptivo de la variabilidad genética de los aislados de C. Sublineolum, el cebador AP1 presentó el mayor valor de contenido de información polimórfica (PIC). La mayor frecuencia alélica se observó en los loci 06, 09, 10 y 24, y la menor en el locus 02. En cuanto al método de agrupamiento, se observó una tendencia a agrupar los aislados de C. sublineolum según el origen geográfico y, además de demostrando la variabilidad genética entre los aislados de C. sublineolum, se observó la ocurrencia de introgresión entre los aislados.A ocorrência de doenças é um fator limitante no desenvolvimento da cultura do sorgo. Dentre as doenças que causam prejuízos na produção de sorgo, a antracnose é a principal e mais severa, principalmente pela variabilidade genética do patógeno. Nesse contexto, o objetivo deste estudo foi avaliar a variabilidade genética de isolados de Colletotrichum sublineolum. O DNA foi extraído de 56 isolados monospóricos de C. sublineolum por meio de um kit de extração de DNA, e para realizar a análise da diversidade genética dos isolados foram utilizados os primers ISSR. Após a amplificação, determinou-se o conteúdo de informação polimórfica (PIC), frequência alélica, análises de agrupamento UPGMA e Tocher e, por meio do software Structure, a estrutura genética. De acordo com a análise descritiva da variabilidade genética dos isolados de C. Sublineolum, o primer AP1 apresentou o maior valor de conteúdo de informação polimórfica (PIC). A maior frequência alélica foi observada nos locos 06, 09, 10 e 24, e a menor no locus 02. Quanto ao método de agrupamento, observou-se uma tendência de agrupar os isolados de C. sublineolum de acordo com a origem geográfica e, além de demonstrando a variabilidade genética entre os isolados de C. sublineolum, foi observada a ocorrência de introgressão entre os isolados.Research, Society and Development2021-02-10info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://rsdjournal.org/index.php/rsd/article/view/1222310.33448/rsd-v10i2.12223Research, Society and Development; Vol. 10 No. 2; e20210212223Research, Society and Development; Vol. 10 Núm. 2; e20210212223Research, Society and Development; v. 10 n. 2; e202102122232525-3409reponame:Research, Society and Developmentinstname:Universidade Federal de Itajubá (UNIFEI)instacron:UNIFEIenghttps://rsdjournal.org/index.php/rsd/article/view/12223/11145Copyright (c) 2021 Thallita Santos Guimarães ; Marco Antonio Aparecido Barelli; Carla Lima Corrêa ; Valvenarg Pereira da Silva; Alex Junior Sandol Floriano ; Nilo Leal Sander ; Aline Vidor Melão Duarte ; Bruno Wagner Zago ; Jackson Lauro Borges Ribeiro ; Flávio Dessaune Tardin ; Dagma Dionísia da Silvahttps://creativecommons.org/licenses/by/4.0info:eu-repo/semantics/openAccessGuimarães , Thallita Santos Barelli, Marco Antonio Aparecido Corrêa , Carla Lima Silva, Valvenarg Pereira da Floriano , Alex Junior Sandol Sander , Nilo Leal Duarte , Aline Vidor Melão Zago , Bruno Wagner Ribeiro , Jackson Lauro Borges Tardin , Flávio Dessaune Silva, Dagma Dionísia da 2021-03-02T09:32:39Zoai:ojs.pkp.sfu.ca:article/12223Revistahttps://rsdjournal.org/index.php/rsd/indexPUBhttps://rsdjournal.org/index.php/rsd/oairsd.articles@gmail.com2525-34092525-3409opendoar:2024-01-17T09:33:51.504223Research, Society and Development - Universidade Federal de Itajubá (UNIFEI)false |
dc.title.none.fl_str_mv |
Genetic variability of Colletotrichum sublineolum through ISSR markers Variabilidad genética de Colletotrichum sublineolum através de marcadores ISSR Variabilidade genética de Colletotrichum sublineolum através de Marcadores ISSR |
title |
Genetic variability of Colletotrichum sublineolum through ISSR markers |
spellingShingle |
Genetic variability of Colletotrichum sublineolum through ISSR markers Guimarães , Thallita Santos Sorghum biocolor (L.) Moench Anthracnose Molecular markes. Sorghum biocolor (L.) Moench Antracnosis Marcadores moleculares. Sorghum biocolor (L.) Moench Antracnose Marcadores moleculares. |
title_short |
Genetic variability of Colletotrichum sublineolum through ISSR markers |
title_full |
Genetic variability of Colletotrichum sublineolum through ISSR markers |
title_fullStr |
Genetic variability of Colletotrichum sublineolum through ISSR markers |
title_full_unstemmed |
Genetic variability of Colletotrichum sublineolum through ISSR markers |
title_sort |
Genetic variability of Colletotrichum sublineolum through ISSR markers |
author |
Guimarães , Thallita Santos |
author_facet |
Guimarães , Thallita Santos Barelli, Marco Antonio Aparecido Corrêa , Carla Lima Silva, Valvenarg Pereira da Floriano , Alex Junior Sandol Sander , Nilo Leal Duarte , Aline Vidor Melão Zago , Bruno Wagner Ribeiro , Jackson Lauro Borges Tardin , Flávio Dessaune Silva, Dagma Dionísia da |
author_role |
author |
author2 |
Barelli, Marco Antonio Aparecido Corrêa , Carla Lima Silva, Valvenarg Pereira da Floriano , Alex Junior Sandol Sander , Nilo Leal Duarte , Aline Vidor Melão Zago , Bruno Wagner Ribeiro , Jackson Lauro Borges Tardin , Flávio Dessaune Silva, Dagma Dionísia da |
author2_role |
author author author author author author author author author author |
dc.contributor.author.fl_str_mv |
Guimarães , Thallita Santos Barelli, Marco Antonio Aparecido Corrêa , Carla Lima Silva, Valvenarg Pereira da Floriano , Alex Junior Sandol Sander , Nilo Leal Duarte , Aline Vidor Melão Zago , Bruno Wagner Ribeiro , Jackson Lauro Borges Tardin , Flávio Dessaune Silva, Dagma Dionísia da |
dc.subject.por.fl_str_mv |
Sorghum biocolor (L.) Moench Anthracnose Molecular markes. Sorghum biocolor (L.) Moench Antracnosis Marcadores moleculares. Sorghum biocolor (L.) Moench Antracnose Marcadores moleculares. |
topic |
Sorghum biocolor (L.) Moench Anthracnose Molecular markes. Sorghum biocolor (L.) Moench Antracnosis Marcadores moleculares. Sorghum biocolor (L.) Moench Antracnose Marcadores moleculares. |
description |
The occurrence of diseases is a limiting factor in the development of sorghum crop. Among the diseases that causes losses in sorghum production, anthracnose is the main and most severe, mainly by the genetic variability of the pathogen. In this context, the aim of this study was to evaluate the genetic variability of Colletotrichum sublineolum isolates. DNA were extracted from 56 monosporic isolates of C. sublineolum using a DNA extraction kit, and to perform the analysis of genetic diversity of the isolates were used ISSR primers. After amplification, it was determined the polymorphic information content (PIC), allelic frequency, UPGMA and Tocher clustering analyzes and, using software Structure, the genetic structure. According to the descriptive analysis of the genetic variability of C. Sublineolum isolates, primer AP1 presented the higher value of polymorphic information content (PIC). The higher allelic frequency was observed in loci 06, 09, 10, and 24, and the lowest in locus 02. As for the clustering method, it was observed a tendency of grouping C. sublineolum isolates according the geographic origin and, in addition to demonstrating the genetic variability between the C. sublineolum isolates, it was observed the occurrence of introgression among the isolates. |
publishDate |
2021 |
dc.date.none.fl_str_mv |
2021-02-10 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
https://rsdjournal.org/index.php/rsd/article/view/12223 10.33448/rsd-v10i2.12223 |
url |
https://rsdjournal.org/index.php/rsd/article/view/12223 |
identifier_str_mv |
10.33448/rsd-v10i2.12223 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
https://rsdjournal.org/index.php/rsd/article/view/12223/11145 |
dc.rights.driver.fl_str_mv |
https://creativecommons.org/licenses/by/4.0 info:eu-repo/semantics/openAccess |
rights_invalid_str_mv |
https://creativecommons.org/licenses/by/4.0 |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Research, Society and Development |
publisher.none.fl_str_mv |
Research, Society and Development |
dc.source.none.fl_str_mv |
Research, Society and Development; Vol. 10 No. 2; e20210212223 Research, Society and Development; Vol. 10 Núm. 2; e20210212223 Research, Society and Development; v. 10 n. 2; e20210212223 2525-3409 reponame:Research, Society and Development instname:Universidade Federal de Itajubá (UNIFEI) instacron:UNIFEI |
instname_str |
Universidade Federal de Itajubá (UNIFEI) |
instacron_str |
UNIFEI |
institution |
UNIFEI |
reponame_str |
Research, Society and Development |
collection |
Research, Society and Development |
repository.name.fl_str_mv |
Research, Society and Development - Universidade Federal de Itajubá (UNIFEI) |
repository.mail.fl_str_mv |
rsd.articles@gmail.com |
_version_ |
1797052783370698752 |