Cytotoxicity and osteogenic potential of experimental medication with calcium hydroxide and activated charcoal

• Autor(a) principal: Gonçalves, Gabriela Sumie Yaguinum
• Data de Publicação: 2021
• Outros Autores: Gregorio, Danielle, Custódio, Isabelly Ribeiro, Maia, Luciana Prado, Piazza, Bruno, Mori, Graziela Garrido
• Tipo de documento: Artigo
• Idioma: eng
• Título da fonte: Research, Society and Development
• Texto Completo: https://rsdjournal.org/index.php/rsd/article/view/14671
• topic: Alkaline phosphatase; Calcium hydroxide; Charcoal; Endodontics.; Fosfatasa alcalina; Hidróxido de calcio; Carbón vegetal; Endodoncia.; Fosfatase alcalina; Hidróxido de cálcio; Carvão vegetal; Endodontia.

Objectives: The aim of this study was to evaluate the cytotoxicity and osteogenic potential of experimental medications containing Calcium Hydroxide (CH) and Activated charcoal (AC). Methodology: Osteoblastic (MC3T3) and fibroblastic (L929) cells were cultivated in 96 well plates (1 x 104 cells/well) and, after 24 h, treated with extracts, according to experimental groups [(Experimental groups: C - Control; CH - CH paste; CH+10%AC - experimental paste 1 (CH paste + 10% AC); CH+5%AC - experimental paste 2 (CH paste + 5% AC)]. Cytotoxicity and potential osteogenic was performed by MTT and alkaline phosphatase activity, respectively, after 1, 3 and 7 days. Results: For intergroups comparisons, ANOVA 2 factors were used, followed by Tukey test (p < 0.05). There was no difference among the pastes for cytotoxicity in both cells (p > 0.05). For osteogenic potential, it was found that all experimental groups stimulated mineralization in relation to control group, except for experimental paste 2 at 7 days. Conclusion: The addition of AC to the CH paste does not modify toxicity and osteogenic properties. Add 10% of AC appears to be more effective than 5%.