Spasmolytic activity of essential oil from Lippia microphylla Cham. (Verbenaceae) is mediated by modulation of Ca2+ signaling on animal and cellular models

Detalhes bibliográficos
Autor(a) principal: Oliveira, Gislaine Alves de
Data de Publicação: 2021
Outros Autores: Pereira, Joedna Cavalcante, Martins, Italo Rossi Roseno, Correia, Ana Carolina de Carvalho, Travassos, Rafael de Almeida, Silva, Maria da Conceição Correia, Souza, Iara Leão Luna de, Tavares, Josean Fechine, Paredes-Gamero, Edgar Julian, Silva, Bagnólia Araújo da
Tipo de documento: Artigo
Idioma: por
Título da fonte: Research, Society and Development
Texto Completo: https://rsdjournal.org/index.php/rsd/article/view/16060
Resumo: Lippia species (Verbenaceae) are widely used on folk medicine to treat respiratory and gastrointestinal disorders. In this work, we evaluated the relaxant effect of the essential oil from Lippia microphylla Cham. (LM‑EO) and its major components on guinea pig ileum as well as elucidate the involved mechanisms. LM-EO inhibited phasic contractions as well as relaxed tonic contractions induced by several contractile agents on guinea pig ileum. Similar results were observed in tests using thymol and carvacrol, the major constituents of LM-EO. Voltage-dependent calcium channel (CaV) blockade was suggested since LM-EO shifted to the right non parallelly CaCl2-induced cumulative contractions and confirmed when LM-EO relaxed the ileum pre contracted by S-(-)-Bay K8644, a selective CaV agonist. On circular layer, LM-EO inhibited phasic contractions, being equipotent to the effect observed in the intact ileum. In the myocytes from ileum longitudinal layer, LM-EO reduced fluorescence intensity induced by histamine, similarly to verapamil (CaV blocker), indicating a decrease in cytosolic concentration of Ca2+ ([Ca2+]c). In conclusion, LM-EO presents a spasmolytic activity on guinea pig ileum by CaV blockade and [Ca2+]c reduction. These results present a rationale for the use of L. microphylla as a potential product to intestinal disorders.
id UNIFEI_646655e07c982a3ecd5a039d5d5fd4bb
oai_identifier_str oai:ojs.pkp.sfu.ca:article/16060
network_acronym_str UNIFEI
network_name_str Research, Society and Development
repository_id_str
spelling Spasmolytic activity of essential oil from Lippia microphylla Cham. (Verbenaceae) is mediated by modulation of Ca2+ signaling on animal and cellular modelsLa actividad espasmolítica del aceite esencial obtenido de Lippia microphylla Cham. (Verbenaceae) está mediada por la modulación de la señalización del calcio en modelos animales y celularesA atividade espasmolítica do óleo essencial obtido de Lippia microphylla Cham. (Verbenaceae) é mediada pela modulação da sinalização do cálcio em modelos animais e celularesLippia microphylla ChamVerbenaceaeÓleo essencialTimolCarvacrolMúsculo liso intestinal.Lippia microphylla ChamVerbenaceaeEssential oilTimolCarvacrolMúsculo liso intestinal.Lippia microphylla ChamVerbenaceaeAceite essencialTimol; CarvacrolMúsculo liso intestinal.Lippia species (Verbenaceae) are widely used on folk medicine to treat respiratory and gastrointestinal disorders. In this work, we evaluated the relaxant effect of the essential oil from Lippia microphylla Cham. (LM‑EO) and its major components on guinea pig ileum as well as elucidate the involved mechanisms. LM-EO inhibited phasic contractions as well as relaxed tonic contractions induced by several contractile agents on guinea pig ileum. Similar results were observed in tests using thymol and carvacrol, the major constituents of LM-EO. Voltage-dependent calcium channel (CaV) blockade was suggested since LM-EO shifted to the right non parallelly CaCl2-induced cumulative contractions and confirmed when LM-EO relaxed the ileum pre contracted by S-(-)-Bay K8644, a selective CaV agonist. On circular layer, LM-EO inhibited phasic contractions, being equipotent to the effect observed in the intact ileum. In the myocytes from ileum longitudinal layer, LM-EO reduced fluorescence intensity induced by histamine, similarly to verapamil (CaV blocker), indicating a decrease in cytosolic concentration of Ca2+ ([Ca2+]c). In conclusion, LM-EO presents a spasmolytic activity on guinea pig ileum by CaV blockade and [Ca2+]c reduction. These results present a rationale for the use of L. microphylla as a potential product to intestinal disorders.Las especies del género Lippia (Verbenaceae) se utilizan ampliamente en la medicina tradicional para tratar desórdenes respiratorios y gastrointestinales. En este estudio, el efecto relajante del aceite esencial obtenido de Lippia microphylla Cham. (LM-EO) y sus componentes principales en el íleon de cobaya, así como los mecanismos implicados. LM-EO inhibió las contracciones fásicas y relajó la tonicidad inducida por varios agentes contráctiles en el íleon de cobaya. Se observaron resultados similares en los protocolos que utilizan timol y carvacrol, los componentes principales de LM-EO. Se sugirió el bloqueo de los canales de calcio dependientes de voltaje (CaV) ya que LM-EO se desplazó hacia la derecha y de manera no paralela las contracciones acumulativas inducidas por CaCl2 y se confirmó cuando LM-EO relajó el íleon precontraído por S‑(‑)‑Bay K8644, un agonista selectivo de CaV. En la capa muscular circular del íleon, LM-EO inhibió las contracciones fásicas, siendo equipotente en relación a los efectos sobre el íleon intacto. En miocitos aislados de la capa de íleon longitudinal, LM-EO redujo la intensidad de la fluorescencia inducida por histamina, de manera similar al verapamilo (bloqueador de CaV), lo que indica una reducción en la concentración de calcio citosólico ([Ca2+]c). Por tanto, LM-EO presenta actividad espasmolítica en el íleo de cobaya al bloquear el CaV y la consiguiente reducción de [Ca2+]c. Estos resultados se muestran como una justificación para el uso de L. microphylla como producto terapéutico en trastornos intestinales.As espécies do gênero Lippia (Verbenaceae) são amplamente usadas na medicina tradicional no tratamento de desordens respiratórias e gastrointestinais. Neste estudo, avaliou-se o efeito relaxante do óleo essencial obtido de Lippia microphylla Cham. (LM‑EO) e seus componentes majoritários em íleo isolado de cobaias bem como elucidou‑se os mecanismos envolvidos. LM-EO inibiu as contrações fásicas e relaxou as tônicas induzidas por vários agentes contráteis em íleo de cobaia. Resultados semelhantes foram observados nos protocolos utilizando-se timol e carvacrol, os componentes majoritários de LM-EO. O bloqueio dos canais de cálcio dependentes de voltagem (CaV) foi sugerido uma vez que LM-EO deslocou para a direita e de maneira não paralela as contrações cumulativas induzidas por CaCl2 e confirmou-se quando LM-EO relaxou o íleo pré-contraído por S‑(‑)‑Bay K8644, um agonista seletivo dos CaV. Na camada muscular circular do íleo, LM-EO inibiu as contrações fásicas, sendo equipotente em relação aos efeitos no íleo íntegro. No miócitos isolados da camada longitudinal do íleo, LM-EO reduziu a intensidade da fluorescência induzida por histamina, semelhantemente ao verapamil (bloqueador CaV), indicando uma redução na concentração citosólica de cálcio ([Ca2+]c). Dessa forma, LM-EO apresenta uma atividade espasmolítica em íleo de cobaia pelo bloqueio dos CaV e consequente redução da [Ca2+]c. Esses resultados demostram-se como uma justificativa para o uso de L. microphylla como um produto terapêutico nas desordens intestinais.Research, Society and Development2021-06-11info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://rsdjournal.org/index.php/rsd/article/view/1606010.33448/rsd-v10i7.16060Research, Society and Development; Vol. 10 No. 7; e0410716060Research, Society and Development; Vol. 10 Núm. 7; e0410716060Research, Society and Development; v. 10 n. 7; e04107160602525-3409reponame:Research, Society and Developmentinstname:Universidade Federal de Itajubá (UNIFEI)instacron:UNIFEIporhttps://rsdjournal.org/index.php/rsd/article/view/16060/14466Copyright (c) 2021 Gislaine Alves de Oliveira; Joedna Cavalcante Pereira; Italo Rossi Roseno Martins; Ana Carolina de Carvalho Correia; Rafael de Almeida Travassos; Maria da Conceição Correia Silva; Iara Leão Luna de Souza; Josean Fechine Tavares; Edgar Julian Paredes-Gamero; Bagnólia Araújo da Silvahttps://creativecommons.org/licenses/by/4.0info:eu-repo/semantics/openAccessOliveira, Gislaine Alves dePereira, Joedna CavalcanteMartins, Italo Rossi RosenoCorreia, Ana Carolina de CarvalhoTravassos, Rafael de AlmeidaSilva, Maria da Conceição CorreiaSouza, Iara Leão Luna deTavares, Josean FechineParedes-Gamero, Edgar Julian Silva, Bagnólia Araújo da2021-07-18T21:07:03Zoai:ojs.pkp.sfu.ca:article/16060Revistahttps://rsdjournal.org/index.php/rsd/indexPUBhttps://rsdjournal.org/index.php/rsd/oairsd.articles@gmail.com2525-34092525-3409opendoar:2024-01-17T09:36:43.540255Research, Society and Development - Universidade Federal de Itajubá (UNIFEI)false
dc.title.none.fl_str_mv Spasmolytic activity of essential oil from Lippia microphylla Cham. (Verbenaceae) is mediated by modulation of Ca2+ signaling on animal and cellular models
La actividad espasmolítica del aceite esencial obtenido de Lippia microphylla Cham. (Verbenaceae) está mediada por la modulación de la señalización del calcio en modelos animales y celulares
A atividade espasmolítica do óleo essencial obtido de Lippia microphylla Cham. (Verbenaceae) é mediada pela modulação da sinalização do cálcio em modelos animais e celulares
title Spasmolytic activity of essential oil from Lippia microphylla Cham. (Verbenaceae) is mediated by modulation of Ca2+ signaling on animal and cellular models
spellingShingle Spasmolytic activity of essential oil from Lippia microphylla Cham. (Verbenaceae) is mediated by modulation of Ca2+ signaling on animal and cellular models
Oliveira, Gislaine Alves de
Lippia microphylla Cham
Verbenaceae
Óleo essencial
Timol
Carvacrol
Músculo liso intestinal.
Lippia microphylla Cham
Verbenaceae
Essential oil
Timol
Carvacrol
Músculo liso intestinal.
Lippia microphylla Cham
Verbenaceae
Aceite essencial
Timol
; Carvacrol
Músculo liso intestinal.
title_short Spasmolytic activity of essential oil from Lippia microphylla Cham. (Verbenaceae) is mediated by modulation of Ca2+ signaling on animal and cellular models
title_full Spasmolytic activity of essential oil from Lippia microphylla Cham. (Verbenaceae) is mediated by modulation of Ca2+ signaling on animal and cellular models
title_fullStr Spasmolytic activity of essential oil from Lippia microphylla Cham. (Verbenaceae) is mediated by modulation of Ca2+ signaling on animal and cellular models
title_full_unstemmed Spasmolytic activity of essential oil from Lippia microphylla Cham. (Verbenaceae) is mediated by modulation of Ca2+ signaling on animal and cellular models
title_sort Spasmolytic activity of essential oil from Lippia microphylla Cham. (Verbenaceae) is mediated by modulation of Ca2+ signaling on animal and cellular models
author Oliveira, Gislaine Alves de
author_facet Oliveira, Gislaine Alves de
Pereira, Joedna Cavalcante
Martins, Italo Rossi Roseno
Correia, Ana Carolina de Carvalho
Travassos, Rafael de Almeida
Silva, Maria da Conceição Correia
Souza, Iara Leão Luna de
Tavares, Josean Fechine
Paredes-Gamero, Edgar Julian
Silva, Bagnólia Araújo da
author_role author
author2 Pereira, Joedna Cavalcante
Martins, Italo Rossi Roseno
Correia, Ana Carolina de Carvalho
Travassos, Rafael de Almeida
Silva, Maria da Conceição Correia
Souza, Iara Leão Luna de
Tavares, Josean Fechine
Paredes-Gamero, Edgar Julian
Silva, Bagnólia Araújo da
author2_role author
author
author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv Oliveira, Gislaine Alves de
Pereira, Joedna Cavalcante
Martins, Italo Rossi Roseno
Correia, Ana Carolina de Carvalho
Travassos, Rafael de Almeida
Silva, Maria da Conceição Correia
Souza, Iara Leão Luna de
Tavares, Josean Fechine
Paredes-Gamero, Edgar Julian
Silva, Bagnólia Araújo da
dc.subject.por.fl_str_mv Lippia microphylla Cham
Verbenaceae
Óleo essencial
Timol
Carvacrol
Músculo liso intestinal.
Lippia microphylla Cham
Verbenaceae
Essential oil
Timol
Carvacrol
Músculo liso intestinal.
Lippia microphylla Cham
Verbenaceae
Aceite essencial
Timol
; Carvacrol
Músculo liso intestinal.
topic Lippia microphylla Cham
Verbenaceae
Óleo essencial
Timol
Carvacrol
Músculo liso intestinal.
Lippia microphylla Cham
Verbenaceae
Essential oil
Timol
Carvacrol
Músculo liso intestinal.
Lippia microphylla Cham
Verbenaceae
Aceite essencial
Timol
; Carvacrol
Músculo liso intestinal.
description Lippia species (Verbenaceae) are widely used on folk medicine to treat respiratory and gastrointestinal disorders. In this work, we evaluated the relaxant effect of the essential oil from Lippia microphylla Cham. (LM‑EO) and its major components on guinea pig ileum as well as elucidate the involved mechanisms. LM-EO inhibited phasic contractions as well as relaxed tonic contractions induced by several contractile agents on guinea pig ileum. Similar results were observed in tests using thymol and carvacrol, the major constituents of LM-EO. Voltage-dependent calcium channel (CaV) blockade was suggested since LM-EO shifted to the right non parallelly CaCl2-induced cumulative contractions and confirmed when LM-EO relaxed the ileum pre contracted by S-(-)-Bay K8644, a selective CaV agonist. On circular layer, LM-EO inhibited phasic contractions, being equipotent to the effect observed in the intact ileum. In the myocytes from ileum longitudinal layer, LM-EO reduced fluorescence intensity induced by histamine, similarly to verapamil (CaV blocker), indicating a decrease in cytosolic concentration of Ca2+ ([Ca2+]c). In conclusion, LM-EO presents a spasmolytic activity on guinea pig ileum by CaV blockade and [Ca2+]c reduction. These results present a rationale for the use of L. microphylla as a potential product to intestinal disorders.
publishDate 2021
dc.date.none.fl_str_mv 2021-06-11
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv https://rsdjournal.org/index.php/rsd/article/view/16060
10.33448/rsd-v10i7.16060
url https://rsdjournal.org/index.php/rsd/article/view/16060
identifier_str_mv 10.33448/rsd-v10i7.16060
dc.language.iso.fl_str_mv por
language por
dc.relation.none.fl_str_mv https://rsdjournal.org/index.php/rsd/article/view/16060/14466
dc.rights.driver.fl_str_mv https://creativecommons.org/licenses/by/4.0
info:eu-repo/semantics/openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by/4.0
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Research, Society and Development
publisher.none.fl_str_mv Research, Society and Development
dc.source.none.fl_str_mv Research, Society and Development; Vol. 10 No. 7; e0410716060
Research, Society and Development; Vol. 10 Núm. 7; e0410716060
Research, Society and Development; v. 10 n. 7; e0410716060
2525-3409
reponame:Research, Society and Development
instname:Universidade Federal de Itajubá (UNIFEI)
instacron:UNIFEI
instname_str Universidade Federal de Itajubá (UNIFEI)
instacron_str UNIFEI
institution UNIFEI
reponame_str Research, Society and Development
collection Research, Society and Development
repository.name.fl_str_mv Research, Society and Development - Universidade Federal de Itajubá (UNIFEI)
repository.mail.fl_str_mv rsd.articles@gmail.com
_version_ 1797052679003832320