Elimination of Giardia muris in naturally infected murine experimental model: Complementary treatment
Autor(a) principal: | |
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Data de Publicação: | 2021 |
Outros Autores: | , , , |
Tipo de documento: | Artigo |
Idioma: | por |
Título da fonte: | Research, Society and Development |
Texto Completo: | https://rsdjournal.org/index.php/rsd/article/view/16996 |
Resumo: | Objective: This study proposed a complementary treatment for murine giardiasis comparing Black tea (CPR) (Camellia sinensis) with Metronidazole (MT), Fembendazole (FB) and Metronidazole in association with Sulfadimetoxin (Mts) in male Swiss mice (30) divided into six groups (CPR, MT, FB/MT, Mts, CPR/Mts and Untreated Control - C+) with five animals each. Methodology: The treatment was intragastric, for 7 days 1x/day and group C+ received only water. The experiments were conducted blindly, controlled, randomized, and repeated once with same number of animals. Clinical parameters (weight, water consumption, feed and excreta elimination) were also evaluated. Results: The animals in the CPR/MtS and MtS groups and in the CPR group presented negative parasitological and molecular results for G. muris on the 5th and 7th day after starting treatment, respectively. The animals in groups FB/MT, MT and C+ continued to eliminate cysts after the end of treatment. The animals of group C+ presented significant weight loss in relation to the animals of the other groups and consumed less feed (p=0.0001) in relation to the animals of groups CPR/MtS, FB/MT and MT. Conclusion: The complementary treatment with black tea alone or associated with MtS was effective to eliminate G. muris, being a promising strategy for murine experimental model. The untreated animals that maintained high parasite load showed significant changes in clinical parameters, showing the importance of eliminating pre-existing infection and increasing the reliability of the results of experiments using these animals. |
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Elimination of Giardia muris in naturally infected murine experimental model: Complementary treatmentEliminación de Giardia muris en modelo experimental murino naturalmente infectado: Tratamiento complementarioEliminação de Giardia muris em modelo experimental murino naturalmente infectado: Tratamento complementarGiardíaseCamellia sinensisCamundongos. GiardiasisCamellia sinensisRatones.GiardiasisCamellia sinensisMice.Objective: This study proposed a complementary treatment for murine giardiasis comparing Black tea (CPR) (Camellia sinensis) with Metronidazole (MT), Fembendazole (FB) and Metronidazole in association with Sulfadimetoxin (Mts) in male Swiss mice (30) divided into six groups (CPR, MT, FB/MT, Mts, CPR/Mts and Untreated Control - C+) with five animals each. Methodology: The treatment was intragastric, for 7 days 1x/day and group C+ received only water. The experiments were conducted blindly, controlled, randomized, and repeated once with same number of animals. Clinical parameters (weight, water consumption, feed and excreta elimination) were also evaluated. Results: The animals in the CPR/MtS and MtS groups and in the CPR group presented negative parasitological and molecular results for G. muris on the 5th and 7th day after starting treatment, respectively. The animals in groups FB/MT, MT and C+ continued to eliminate cysts after the end of treatment. The animals of group C+ presented significant weight loss in relation to the animals of the other groups and consumed less feed (p=0.0001) in relation to the animals of groups CPR/MtS, FB/MT and MT. Conclusion: The complementary treatment with black tea alone or associated with MtS was effective to eliminate G. muris, being a promising strategy for murine experimental model. The untreated animals that maintained high parasite load showed significant changes in clinical parameters, showing the importance of eliminating pre-existing infection and increasing the reliability of the results of experiments using these animals.Objetivo: En este estudio se propuso un tratamiento complementario para giardiasis murina comparando Té negro (CPR) (Camellia sinensis), Metronidazol (MT), Fembendazol (FB), Metronidazol en asociación con Sulfadimetoxina (Mts) y en ratones suizos machos (30) divididos en seis grupos (CPR, MT, MT/FB, Mts, CPR/Mts y Control no Tratado - C+) con cinco animales cada uno. Metodología: El tratamiento fue vía intragástrica, por 7 días 1x/día y el grupo C+ recibió solo agua. Los experimentos fueron conducidos de forma ciega, controlada, aleatoria y repetida una vez con el mismo número de animales. También se evaluaron parámetros clínicos (peso, consumo de agua, alimentación y eliminación de excretas). Resultados: Los animales de los grupos CPR/MtS y MtS y del grupo CPR presentaron resultados parasitológicos y moleculares negativos para G. muris al 5º y 7º día de iniciado el tratamiento, respectivamente. Los animales de los grupos FB/MT, MT y C+ siguieron eliminando quistes tras el final del tratamiento. Los animales del grupo C+ presentaron una pérdida de peso significativa en relación con los animales de los otros grupos y consumieron menos alimento (p=0,0001) en relación con los animales de los grupos CPR/MtS, FB/MT y MT. Conclusión: El tratamiento complementario con chá preto aislado o asociado a MtS fue eficaz para eliminar G. muris, siendo una estrategia prometedora para el modelo experimental murino. Los animales no tratados que mantenían una alta carga parasitaria mostraron cambios significativos en los parámetros clínicos, lo que demuestra la importancia de eliminar la infección preexistente y aumenta la fiabilidad de los resultados de los experimentos con estos animales.Objetivo: Neste estudo foi proposto um tratamento complementar para giardíase murina comparando Chá preto (CPR) (Camellia sinensis) com Metronidazol (MT), Fembendazol (FB) e Metronidazol em associação com Sulfadimetoxina (MtS) em camundongos Swiss machos (30) divididos em seis grupos (CPR, MT, FB/MT, MtS, CPR/MtS e Controle não Tratado - C+) com cinco animais cada. Metodologia: O tratamento foi via intragástrica, por 7 dias 1x/dia e o grupo C+ recebeu apenas água. Os experimentos foram conduzidos de forma cega, controlada, randomizada e repetido uma vez com mesmo número de animais. Parâmetros clínicos (peso, consumo de água, ração e eliminação de excretas) também foram avaliados. Resultados: Os animais dos grupos CPR/MtS e MtS e do grupo CPR apresentaram resultados parasitológicos e molecular negativos para G. muris no 5º e 7º dia após início do tratamento, respectivamente. Os animais dos grupos FB/MT, MT e C+ continuaram a eliminar cistos após o término do tratamento. Os animais do grupo C+ apresentaram perda de peso significativa em relação aos animais dos demais grupos e consumiram menor quantidade de ração (p=0,0001) em relação aos animais dos grupos CPR/MtS, FB/MT e MT. Conclusão: O tratamento complementar com chá preto isolado ou associado ao MtS foi eficaz para eliminar G. muris, sendo uma estratégia promissora para modelo experimental murino. Os animais não tratados que mantiveram alta carga parasitária apresentaram alterações significativas nos parâmetros clínicos, mostrando a importância de eliminar infecção pré-existente e aumentar a confiabilidade dos resultados dos experimentos que utilizam esses animais.Research, Society and Development2021-07-02info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://rsdjournal.org/index.php/rsd/article/view/1699610.33448/rsd-v10i7.16996Research, Society and Development; Vol. 10 No. 7; e60010716996Research, Society and Development; Vol. 10 Núm. 7; e60010716996Research, Society and Development; v. 10 n. 7; e600107169962525-3409reponame:Research, Society and Developmentinstname:Universidade Federal de Itajubá (UNIFEI)instacron:UNIFEIporhttps://rsdjournal.org/index.php/rsd/article/view/16996/15126Copyright (c) 2021 Caroline Rodrigues de Almeida; Renata Coltro Bezagio ; Cristiane Maria Colli; Liara Izabela Lopes Romera; Mônica Lúcia Gomes https://creativecommons.org/licenses/by/4.0info:eu-repo/semantics/openAccessAlmeida, Caroline Rodrigues de Bezagio , Renata ColtroColli, Cristiane MariaRomera, Liara Izabela LopesGomes , Mônica Lúcia2021-07-18T21:07:03Zoai:ojs.pkp.sfu.ca:article/16996Revistahttps://rsdjournal.org/index.php/rsd/indexPUBhttps://rsdjournal.org/index.php/rsd/oairsd.articles@gmail.com2525-34092525-3409opendoar:2024-01-17T09:37:24.301238Research, Society and Development - Universidade Federal de Itajubá (UNIFEI)false |
dc.title.none.fl_str_mv |
Elimination of Giardia muris in naturally infected murine experimental model: Complementary treatment Eliminación de Giardia muris en modelo experimental murino naturalmente infectado: Tratamiento complementario Eliminação de Giardia muris em modelo experimental murino naturalmente infectado: Tratamento complementar |
title |
Elimination of Giardia muris in naturally infected murine experimental model: Complementary treatment |
spellingShingle |
Elimination of Giardia muris in naturally infected murine experimental model: Complementary treatment Almeida, Caroline Rodrigues de Giardíase Camellia sinensis Camundongos. Giardiasis Camellia sinensis Ratones. Giardiasis Camellia sinensis Mice. |
title_short |
Elimination of Giardia muris in naturally infected murine experimental model: Complementary treatment |
title_full |
Elimination of Giardia muris in naturally infected murine experimental model: Complementary treatment |
title_fullStr |
Elimination of Giardia muris in naturally infected murine experimental model: Complementary treatment |
title_full_unstemmed |
Elimination of Giardia muris in naturally infected murine experimental model: Complementary treatment |
title_sort |
Elimination of Giardia muris in naturally infected murine experimental model: Complementary treatment |
author |
Almeida, Caroline Rodrigues de |
author_facet |
Almeida, Caroline Rodrigues de Bezagio , Renata Coltro Colli, Cristiane Maria Romera, Liara Izabela Lopes Gomes , Mônica Lúcia |
author_role |
author |
author2 |
Bezagio , Renata Coltro Colli, Cristiane Maria Romera, Liara Izabela Lopes Gomes , Mônica Lúcia |
author2_role |
author author author author |
dc.contributor.author.fl_str_mv |
Almeida, Caroline Rodrigues de Bezagio , Renata Coltro Colli, Cristiane Maria Romera, Liara Izabela Lopes Gomes , Mônica Lúcia |
dc.subject.por.fl_str_mv |
Giardíase Camellia sinensis Camundongos. Giardiasis Camellia sinensis Ratones. Giardiasis Camellia sinensis Mice. |
topic |
Giardíase Camellia sinensis Camundongos. Giardiasis Camellia sinensis Ratones. Giardiasis Camellia sinensis Mice. |
description |
Objective: This study proposed a complementary treatment for murine giardiasis comparing Black tea (CPR) (Camellia sinensis) with Metronidazole (MT), Fembendazole (FB) and Metronidazole in association with Sulfadimetoxin (Mts) in male Swiss mice (30) divided into six groups (CPR, MT, FB/MT, Mts, CPR/Mts and Untreated Control - C+) with five animals each. Methodology: The treatment was intragastric, for 7 days 1x/day and group C+ received only water. The experiments were conducted blindly, controlled, randomized, and repeated once with same number of animals. Clinical parameters (weight, water consumption, feed and excreta elimination) were also evaluated. Results: The animals in the CPR/MtS and MtS groups and in the CPR group presented negative parasitological and molecular results for G. muris on the 5th and 7th day after starting treatment, respectively. The animals in groups FB/MT, MT and C+ continued to eliminate cysts after the end of treatment. The animals of group C+ presented significant weight loss in relation to the animals of the other groups and consumed less feed (p=0.0001) in relation to the animals of groups CPR/MtS, FB/MT and MT. Conclusion: The complementary treatment with black tea alone or associated with MtS was effective to eliminate G. muris, being a promising strategy for murine experimental model. The untreated animals that maintained high parasite load showed significant changes in clinical parameters, showing the importance of eliminating pre-existing infection and increasing the reliability of the results of experiments using these animals. |
publishDate |
2021 |
dc.date.none.fl_str_mv |
2021-07-02 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
https://rsdjournal.org/index.php/rsd/article/view/16996 10.33448/rsd-v10i7.16996 |
url |
https://rsdjournal.org/index.php/rsd/article/view/16996 |
identifier_str_mv |
10.33448/rsd-v10i7.16996 |
dc.language.iso.fl_str_mv |
por |
language |
por |
dc.relation.none.fl_str_mv |
https://rsdjournal.org/index.php/rsd/article/view/16996/15126 |
dc.rights.driver.fl_str_mv |
https://creativecommons.org/licenses/by/4.0 info:eu-repo/semantics/openAccess |
rights_invalid_str_mv |
https://creativecommons.org/licenses/by/4.0 |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Research, Society and Development |
publisher.none.fl_str_mv |
Research, Society and Development |
dc.source.none.fl_str_mv |
Research, Society and Development; Vol. 10 No. 7; e60010716996 Research, Society and Development; Vol. 10 Núm. 7; e60010716996 Research, Society and Development; v. 10 n. 7; e60010716996 2525-3409 reponame:Research, Society and Development instname:Universidade Federal de Itajubá (UNIFEI) instacron:UNIFEI |
instname_str |
Universidade Federal de Itajubá (UNIFEI) |
instacron_str |
UNIFEI |
institution |
UNIFEI |
reponame_str |
Research, Society and Development |
collection |
Research, Society and Development |
repository.name.fl_str_mv |
Research, Society and Development - Universidade Federal de Itajubá (UNIFEI) |
repository.mail.fl_str_mv |
rsd.articles@gmail.com |
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1797052751408005120 |