Monitoring the stability of the xanthophyll fucoxanthin in microalga and seaweed biomasses, and extracts stored at low temperatures

Bibliographic Details
Main Author: Schmitz, Caroline
Publication Date: 2022
Other Authors: Nunes, Aline, Bauer , Cláudia Marlene, Bonomi-Barufi, José, Maraschin, Marcelo
Format: Article
Language: eng
Source: Research, Society and Development
Download full: https://rsdjournal.org/index.php/rsd/article/view/37712
Summary: The analysis of fucoxanthin (FCX) in algal biomasses presents some limitations, especially regarding the sample preservation after collection until extraction of the pigment. FCX stability was evaluated in biomass samples of one diatom and three brown seaweed species (Phaeophyceae) and their extracts stored at -20ºC and -80ºC, for 60 days. Fucoxanthin was quantified through UHPLC-DAD. The methanolic extracts of the micro/macroalga biomasses stored at -20ºC and -80ºC were more effective in preserving the xanthophyll, comparatively to the algal biomasses. Besides, the chromatographic analysis showed an increase in the FCX’s peak retention time along the sample storage, possibly due to the trans→cis isomerization of the molecule. Such information is relevant for choosing the best protocol for storing and preserving at most FCX, because changes in the geometric conformations of the molecule over storage strongly affects its biological activities.
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spelling Monitoring the stability of the xanthophyll fucoxanthin in microalga and seaweed biomasses, and extracts stored at low temperaturesMonitoreo de la estabilidad de la xantofila fucoxantina en biomasas de microalgas y algas y extractos almacenados a bajas temperaturasMonitoramento da estabilidade da xantofila fucoxantina em biomassas de microalga e algas marinhas e extratos armazenados a baixas temperaturasPhaeophyceaeFucoxanthin isomersCarotenoidsUHPLCBacillariophyceae.PhaeophyceaeIsómeros de FucoxantinaCarotenoidesUHPLCBacillariophyceae.PhaeophyceaeIsômeros de FucoxantinaCarotenoidesUHPLCBacillariophyceae.The analysis of fucoxanthin (FCX) in algal biomasses presents some limitations, especially regarding the sample preservation after collection until extraction of the pigment. FCX stability was evaluated in biomass samples of one diatom and three brown seaweed species (Phaeophyceae) and their extracts stored at -20ºC and -80ºC, for 60 days. Fucoxanthin was quantified through UHPLC-DAD. The methanolic extracts of the micro/macroalga biomasses stored at -20ºC and -80ºC were more effective in preserving the xanthophyll, comparatively to the algal biomasses. Besides, the chromatographic analysis showed an increase in the FCX’s peak retention time along the sample storage, possibly due to the trans→cis isomerization of the molecule. Such information is relevant for choosing the best protocol for storing and preserving at most FCX, because changes in the geometric conformations of the molecule over storage strongly affects its biological activities.El análisis de fucoxantina (FCX) en biomasas de algas presenta algunas limitaciones, especialmente en lo que se refiere a la conservación de la muestra después de su recolección hasta la extracción del pigmento. La estabilidad de FCX se evaluó en muestras de biomasa y extracto de una diatomea y tres especies de algas marrón (Phaeophyceae) almacenados a -20ºC y -80ºC, durante 60 días. La FCX se cuantificó mediante UHPLC-DAD. Los extractos metanólicos de las biomasas de micro/macroalgas almacenados a -20ºC y -80ºC fueron más efectivos en la conservación de FCX, en comparación a las biomasas de algas. Además, el análisis cromatográfico mostró un aumento del tiempo de retención del pico de FCX a lo largo del almacenamiento de la muestra, posiblemente debido a la isomerización trans→cis de la molécula. Esta información es relevante para elegir el mejor protocolo de almacenamiento y preservacion de FCX, porque los cambios de conformaciones geométricas de la molécula durante el almacenamiento afectam fuertemente sus actividades biológicas.A análise de fucoxantina (FCX) em biomassa de algas apresenta algumas limitações, principalmente quanto à preservação das amostras após a coleta até a extração do pigmento. A estabilidade da FCX foi avaliada em amostras de biomassas e extrato de uma diatomacea e três espécies de macroalgas marrons (Phaeophyceae) armazenadas à -20ºC e -80ºC, por 60 dias. Os conteúdos de FCX nas amostras foram determinados por UHPLC-DAD. Os extratos metanólicos das biomassas de micro/macroalgas armazenadas à -20ºC e -80ºC foram mais eficazes na preservação da xantofila, comparativamente às biomassas algais. A análise cromatográfica mostrou um aumento no tempo de retenção do pico de FCX nas amostras ao longo do armazenamento, possivelmente devido à isomerização trans→cis da molécula. Tal informação é relevante à escolha do melhor protocolo de armazenamento para preservar ao máximo os teores daquele oxicarotenoide, porque alterações nas conformações geométricas da molécula ao longo do armazenamento impactam fortemente nas atividades biológicas da FCX.Research, Society and Development2022-11-26info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://rsdjournal.org/index.php/rsd/article/view/3771210.33448/rsd-v11i15.37712Research, Society and Development; Vol. 11 No. 15; e577111537712Research, Society and Development; Vol. 11 Núm. 15; e577111537712Research, Society and Development; v. 11 n. 15; e5771115377122525-3409reponame:Research, Society and Developmentinstname:Universidade Federal de Itajubá (UNIFEI)instacron:UNIFEIenghttps://rsdjournal.org/index.php/rsd/article/view/37712/31305Copyright (c) 2022 Caroline Schmitz; Aline Nunes; Cláudia Marlene Bauer ; José Bonomi-Barufi; Marcelo Maraschinhttps://creativecommons.org/licenses/by/4.0info:eu-repo/semantics/openAccessSchmitz, CarolineNunes, AlineBauer , Cláudia Marlene Bonomi-Barufi, JoséMaraschin, Marcelo2022-11-27T19:56:23Zoai:ojs.pkp.sfu.ca:article/37712Revistahttps://rsdjournal.org/index.php/rsd/indexPUBhttps://rsdjournal.org/index.php/rsd/oairsd.articles@gmail.com2525-34092525-3409opendoar:2024-01-17T09:51:46.687901Research, Society and Development - Universidade Federal de Itajubá (UNIFEI)false
dc.title.none.fl_str_mv Monitoring the stability of the xanthophyll fucoxanthin in microalga and seaweed biomasses, and extracts stored at low temperatures
Monitoreo de la estabilidad de la xantofila fucoxantina en biomasas de microalgas y algas y extractos almacenados a bajas temperaturas
Monitoramento da estabilidade da xantofila fucoxantina em biomassas de microalga e algas marinhas e extratos armazenados a baixas temperaturas
title Monitoring the stability of the xanthophyll fucoxanthin in microalga and seaweed biomasses, and extracts stored at low temperatures
spellingShingle Monitoring the stability of the xanthophyll fucoxanthin in microalga and seaweed biomasses, and extracts stored at low temperatures
Schmitz, Caroline
Phaeophyceae
Fucoxanthin isomers
Carotenoids
UHPLC
Bacillariophyceae.
Phaeophyceae
Isómeros de Fucoxantina
Carotenoides
UHPLC
Bacillariophyceae.
Phaeophyceae
Isômeros de Fucoxantina
Carotenoides
UHPLC
Bacillariophyceae.
title_short Monitoring the stability of the xanthophyll fucoxanthin in microalga and seaweed biomasses, and extracts stored at low temperatures
title_full Monitoring the stability of the xanthophyll fucoxanthin in microalga and seaweed biomasses, and extracts stored at low temperatures
title_fullStr Monitoring the stability of the xanthophyll fucoxanthin in microalga and seaweed biomasses, and extracts stored at low temperatures
title_full_unstemmed Monitoring the stability of the xanthophyll fucoxanthin in microalga and seaweed biomasses, and extracts stored at low temperatures
title_sort Monitoring the stability of the xanthophyll fucoxanthin in microalga and seaweed biomasses, and extracts stored at low temperatures
author Schmitz, Caroline
author_facet Schmitz, Caroline
Nunes, Aline
Bauer , Cláudia Marlene
Bonomi-Barufi, José
Maraschin, Marcelo
author_role author
author2 Nunes, Aline
Bauer , Cláudia Marlene
Bonomi-Barufi, José
Maraschin, Marcelo
author2_role author
author
author
author
dc.contributor.author.fl_str_mv Schmitz, Caroline
Nunes, Aline
Bauer , Cláudia Marlene
Bonomi-Barufi, José
Maraschin, Marcelo
dc.subject.por.fl_str_mv Phaeophyceae
Fucoxanthin isomers
Carotenoids
UHPLC
Bacillariophyceae.
Phaeophyceae
Isómeros de Fucoxantina
Carotenoides
UHPLC
Bacillariophyceae.
Phaeophyceae
Isômeros de Fucoxantina
Carotenoides
UHPLC
Bacillariophyceae.
topic Phaeophyceae
Fucoxanthin isomers
Carotenoids
UHPLC
Bacillariophyceae.
Phaeophyceae
Isómeros de Fucoxantina
Carotenoides
UHPLC
Bacillariophyceae.
Phaeophyceae
Isômeros de Fucoxantina
Carotenoides
UHPLC
Bacillariophyceae.
description The analysis of fucoxanthin (FCX) in algal biomasses presents some limitations, especially regarding the sample preservation after collection until extraction of the pigment. FCX stability was evaluated in biomass samples of one diatom and three brown seaweed species (Phaeophyceae) and their extracts stored at -20ºC and -80ºC, for 60 days. Fucoxanthin was quantified through UHPLC-DAD. The methanolic extracts of the micro/macroalga biomasses stored at -20ºC and -80ºC were more effective in preserving the xanthophyll, comparatively to the algal biomasses. Besides, the chromatographic analysis showed an increase in the FCX’s peak retention time along the sample storage, possibly due to the trans→cis isomerization of the molecule. Such information is relevant for choosing the best protocol for storing and preserving at most FCX, because changes in the geometric conformations of the molecule over storage strongly affects its biological activities.
publishDate 2022
dc.date.none.fl_str_mv 2022-11-26
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv https://rsdjournal.org/index.php/rsd/article/view/37712
10.33448/rsd-v11i15.37712
url https://rsdjournal.org/index.php/rsd/article/view/37712
identifier_str_mv 10.33448/rsd-v11i15.37712
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv https://rsdjournal.org/index.php/rsd/article/view/37712/31305
dc.rights.driver.fl_str_mv https://creativecommons.org/licenses/by/4.0
info:eu-repo/semantics/openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by/4.0
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Research, Society and Development
publisher.none.fl_str_mv Research, Society and Development
dc.source.none.fl_str_mv Research, Society and Development; Vol. 11 No. 15; e577111537712
Research, Society and Development; Vol. 11 Núm. 15; e577111537712
Research, Society and Development; v. 11 n. 15; e577111537712
2525-3409
reponame:Research, Society and Development
instname:Universidade Federal de Itajubá (UNIFEI)
instacron:UNIFEI
instname_str Universidade Federal de Itajubá (UNIFEI)
instacron_str UNIFEI
institution UNIFEI
reponame_str Research, Society and Development
collection Research, Society and Development
repository.name.fl_str_mv Research, Society and Development - Universidade Federal de Itajubá (UNIFEI)
repository.mail.fl_str_mv rsd.articles@gmail.com
_version_ 1797052730012860416

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