Xenogenic bone grafting biomaterials do not interfere in the viability and proliferation of stem cells from human exfoliated deciduous teeth - an in vitro pilot study

• Autor(a) principal: Stroparo, Jeferson Luis de Oliveira
• Data de Publicação: 2021
• Outros Autores: Weiss, Suyany Gabriely, Fonseca, Sabrina Cunha da, Spisila, Lisley Janowski, Gonzaga, Carla Castiglia, Oliveira, Gabriel Camargo de, Brotto , Gabriela Loewen, Swiech, Alice Maria, Vieira, Eduardo Discher, Leão Neto, Roberto da Rocha, Franco, Célia Regina Cavichiolo, Leão, Moira Pedroso, Deliberador, Tatiana Miranda, Gabardo, Marilisa Carneiro Leão, Zielak, João César
• Tipo de documento: Artigo
• Idioma: eng
• Título da fonte: Research, Society and Development
• Texto Completo: https://rsdjournal.org/index.php/rsd/article/view/14249
• topic: Células madre; Biomateriales; Pulpa dental.; Células-tronco; Biomateriais; Polpa dentária.; Stem cells; Biomaterials; Dental pulp.

Aim: In vitro evaluation of the influence of bovine xenogenic biomaterials on stem cells from human exfoliated deciduous teeth (SHEDs). The study was divided into three groups: 1) group C (control), containing only MSCs; 2) group BP, containing MSCs and Bonefill Porous®; 3) group BO, containing MSCs and Bio-Oss®. MSCs were derived from a deciduous tooth from a 7-year-old male donor. An aliquot of cells was subjected to immunophenotyping by flow cytometry. Cell viability (neutral red), cytotoxicity (MTT), and cell proliferation (crystal violet) assays were performed. All groups underwent morphological analysis by light microscopy (LM), and the biomaterial with superior performance was submitted to evaluation by scanning electron microscopy (SEM). Time points of 24, 48, and 72 h of culture were used. All results were evaluated with a significance level of 0.05. Results showed that both biomaterials maintained cell viability and cytotoxicity similar to the control. The BO group showed smaller cell proliferation compared to the other groups. In LM evaluation, the BP group showed more spread and adherent cells than the BO group. In SEM, cells of the BP group showed characteristics of more active cells than those of the control. Bovine xenogenic biomaterials positively influenced SHEDs, while the BP group seemed to present higher potential with SHEDs for future application within in vivo and/or clinical studies.