In vitro multiplication of woody bamboo in the Southwestern Amazon, Acre State, Brazil

Detalhes bibliográficos
Autor(a) principal: Leão, João Ricardo Avelino
Data de Publicação: 2022
Outros Autores: Silva, Ana Claudia Lopes da, Sampaio, Paulo de Tarso Barbosa, Raposo, Andréa
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Research, Society and Development
Texto Completo: https://rsdjournal.org/index.php/rsd/article/view/31099
Resumo: Bamboos from the genus Guadua in the Southwestern Amazon basin are difficult to propagate because of their recalcitrant characteristics. This study aimed to evaluate the action of 6-benzylaminopurine (BA) during in vitro multiplication of G. latifolia in a laboratory for clonal seedling production. In vitro multiplication was performed using the cytokinin 6-benzylaminopurine at concentrations of 0, 2, 4, 6, 8 mg L-1 in glass tubes containing liquid medium, incubated for 38 days. The analyzed variables were shoot height, number of shoots, multiplication rate, and the presence of callus and roots in two consecutive subcultures. The use of 6-benzylaminopurine during in vitro multiplication was effective at increasing the number of shoots in the first and second subculture. Shoot height was not influenced by the cytokinin in the culture with a maximum multiplication rate of 4.44 shoots. Therefore, the use of 6 mg L-1 of BA is recommended during in vitro multiplication and to obtain a high multipication rate.
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spelling In vitro multiplication of woody bamboo in the Southwestern Amazon, Acre State, BrazilMultiplicación in vitro de bambú leñoso en la Amazonía Sudoccidental, Acre, Brasil Multiplicação in vitro de bamboo lenhoso na Amazônia Sul-Ocidental, Acre, BrasilGuadua latifoliaMicropropagation6-benzilaminopurineCytokinin.Guadua latifoliaMicropropagación6-bencilaminopurinaCitoquinina.Guadua latifoliaMicropropagação6-benzilaminopurinaCitocinina.Bamboos from the genus Guadua in the Southwestern Amazon basin are difficult to propagate because of their recalcitrant characteristics. This study aimed to evaluate the action of 6-benzylaminopurine (BA) during in vitro multiplication of G. latifolia in a laboratory for clonal seedling production. In vitro multiplication was performed using the cytokinin 6-benzylaminopurine at concentrations of 0, 2, 4, 6, 8 mg L-1 in glass tubes containing liquid medium, incubated for 38 days. The analyzed variables were shoot height, number of shoots, multiplication rate, and the presence of callus and roots in two consecutive subcultures. The use of 6-benzylaminopurine during in vitro multiplication was effective at increasing the number of shoots in the first and second subculture. Shoot height was not influenced by the cytokinin in the culture with a maximum multiplication rate of 4.44 shoots. Therefore, the use of 6 mg L-1 of BA is recommended during in vitro multiplication and to obtain a high multipication rate.Los bambús del género Guadua de la Amazonía Sudoccidental son difíciles de propagar debido a sus características recalcitrantes. El objetivo de este estudio fue evaluar la acción de la 6-bencilaminopurina (BAP) sobre la multiplicación de bambú leñoso G. latifolia en el laboratorio con vistas a la producción de plántulas clonales. La multiplicación in vitro se realizó con 6-bencilaminopurina a concentraciones de 0, 2, 4, 6 y 8 mg L-1 en probetas con medio líquido durante 38 días de incubación. Las variables analizadas fueron número y altura de brotes, tasa de multiplicación, presencia de callo y raíz en dos subcultivos consecutivos. El uso de 6-bencilaminopurina fue eficiente durante la multiplicación in vitro para aumentar el número de brotes durante el primer y segundo subcultivo. La altura de los brotes no fue influenciada por la adición de citoquinina en el cultivo y la tasa de multiplicación fue de 4.44 brotes. Por lo tanto, se recomienda utilizar 6 mg L-1 de BAP durante la multiplicación in vitro y también para lograr una alta tasa de multiplicación.Os bambus do gênero Guadua da Amazônia Sul-Ocidental são de difícil propagação devido suas características recalcitrantes. O objetivo deste estudo foi avaliar a ação da 6-benzilaminopurina (BAP) na multiplicação do bambu lenhoso G. latifolia em laboratório visando a produção de mudas clonais. A multiplicação in vitro foi realizada com 6-benzilaminopurina nas concentrações de 0, 2, 4, 6 e 8 mg L-1 em tubos de ensaio contendo meio líquido durante 38 dias de incubação. As variáveis analisadas foram número e altura de brotos, taxa de multiplicação, presença de calos e de raízes em dois subcultivos consecutivos. O uso de 6-benzilaminopurina foi eficiente durante a multiplicação in vitro influenciando no incremento do número de brotos durante o primeiro e o segundo subcultivo. A altura de brotos não foi influenciada pela adição da citocinina no cultivo e a taxa de multiplicação foi de 4,44 brotos. Portanto, recomenda-se o uso de 6 mg L-1 de BAP durante a multiplicação in vitro e também para se alcançar uma alta taxa de multiplicação de G. latifolia.Research, Society and Development2022-07-03info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://rsdjournal.org/index.php/rsd/article/view/3109910.33448/rsd-v11i9.31099Research, Society and Development; Vol. 11 No. 9; e9411931099Research, Society and Development; Vol. 11 Núm. 9; e9411931099Research, Society and Development; v. 11 n. 9; e94119310992525-3409reponame:Research, Society and Developmentinstname:Universidade Federal de Itajubá (UNIFEI)instacron:UNIFEIenghttps://rsdjournal.org/index.php/rsd/article/view/31099/26938Copyright (c) 2022 João Ricardo Avelino Leão; Ana Claudia Lopes da Silva; Paulo de Tarso Barbosa Sampaio; Andréa Raposohttps://creativecommons.org/licenses/by/4.0info:eu-repo/semantics/openAccessLeão, João Ricardo Avelino Silva, Ana Claudia Lopes da Sampaio, Paulo de Tarso Barbosa Raposo, Andréa 2022-07-21T12:36:16Zoai:ojs.pkp.sfu.ca:article/31099Revistahttps://rsdjournal.org/index.php/rsd/indexPUBhttps://rsdjournal.org/index.php/rsd/oairsd.articles@gmail.com2525-34092525-3409opendoar:2024-01-17T09:47:34.819849Research, Society and Development - Universidade Federal de Itajubá (UNIFEI)false
dc.title.none.fl_str_mv In vitro multiplication of woody bamboo in the Southwestern Amazon, Acre State, Brazil
Multiplicación in vitro de bambú leñoso en la Amazonía Sudoccidental, Acre, Brasil
Multiplicação in vitro de bamboo lenhoso na Amazônia Sul-Ocidental, Acre, Brasil
title In vitro multiplication of woody bamboo in the Southwestern Amazon, Acre State, Brazil
spellingShingle In vitro multiplication of woody bamboo in the Southwestern Amazon, Acre State, Brazil
Leão, João Ricardo Avelino
Guadua latifolia
Micropropagation
6-benzilaminopurine
Cytokinin.
Guadua latifolia
Micropropagación
6-bencilaminopurina
Citoquinina.
Guadua latifolia
Micropropagação
6-benzilaminopurina
Citocinina.
title_short In vitro multiplication of woody bamboo in the Southwestern Amazon, Acre State, Brazil
title_full In vitro multiplication of woody bamboo in the Southwestern Amazon, Acre State, Brazil
title_fullStr In vitro multiplication of woody bamboo in the Southwestern Amazon, Acre State, Brazil
title_full_unstemmed In vitro multiplication of woody bamboo in the Southwestern Amazon, Acre State, Brazil
title_sort In vitro multiplication of woody bamboo in the Southwestern Amazon, Acre State, Brazil
author Leão, João Ricardo Avelino
author_facet Leão, João Ricardo Avelino
Silva, Ana Claudia Lopes da
Sampaio, Paulo de Tarso Barbosa
Raposo, Andréa
author_role author
author2 Silva, Ana Claudia Lopes da
Sampaio, Paulo de Tarso Barbosa
Raposo, Andréa
author2_role author
author
author
dc.contributor.author.fl_str_mv Leão, João Ricardo Avelino
Silva, Ana Claudia Lopes da
Sampaio, Paulo de Tarso Barbosa
Raposo, Andréa
dc.subject.por.fl_str_mv Guadua latifolia
Micropropagation
6-benzilaminopurine
Cytokinin.
Guadua latifolia
Micropropagación
6-bencilaminopurina
Citoquinina.
Guadua latifolia
Micropropagação
6-benzilaminopurina
Citocinina.
topic Guadua latifolia
Micropropagation
6-benzilaminopurine
Cytokinin.
Guadua latifolia
Micropropagación
6-bencilaminopurina
Citoquinina.
Guadua latifolia
Micropropagação
6-benzilaminopurina
Citocinina.
description Bamboos from the genus Guadua in the Southwestern Amazon basin are difficult to propagate because of their recalcitrant characteristics. This study aimed to evaluate the action of 6-benzylaminopurine (BA) during in vitro multiplication of G. latifolia in a laboratory for clonal seedling production. In vitro multiplication was performed using the cytokinin 6-benzylaminopurine at concentrations of 0, 2, 4, 6, 8 mg L-1 in glass tubes containing liquid medium, incubated for 38 days. The analyzed variables were shoot height, number of shoots, multiplication rate, and the presence of callus and roots in two consecutive subcultures. The use of 6-benzylaminopurine during in vitro multiplication was effective at increasing the number of shoots in the first and second subculture. Shoot height was not influenced by the cytokinin in the culture with a maximum multiplication rate of 4.44 shoots. Therefore, the use of 6 mg L-1 of BA is recommended during in vitro multiplication and to obtain a high multipication rate.
publishDate 2022
dc.date.none.fl_str_mv 2022-07-03
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv https://rsdjournal.org/index.php/rsd/article/view/31099
10.33448/rsd-v11i9.31099
url https://rsdjournal.org/index.php/rsd/article/view/31099
identifier_str_mv 10.33448/rsd-v11i9.31099
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv https://rsdjournal.org/index.php/rsd/article/view/31099/26938
dc.rights.driver.fl_str_mv https://creativecommons.org/licenses/by/4.0
info:eu-repo/semantics/openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by/4.0
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Research, Society and Development
publisher.none.fl_str_mv Research, Society and Development
dc.source.none.fl_str_mv Research, Society and Development; Vol. 11 No. 9; e9411931099
Research, Society and Development; Vol. 11 Núm. 9; e9411931099
Research, Society and Development; v. 11 n. 9; e9411931099
2525-3409
reponame:Research, Society and Development
instname:Universidade Federal de Itajubá (UNIFEI)
instacron:UNIFEI
instname_str Universidade Federal de Itajubá (UNIFEI)
instacron_str UNIFEI
institution UNIFEI
reponame_str Research, Society and Development
collection Research, Society and Development
repository.name.fl_str_mv Research, Society and Development - Universidade Federal de Itajubá (UNIFEI)
repository.mail.fl_str_mv rsd.articles@gmail.com
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