Evaluation of the antifungal activity of propolis extracts from stingless bees on phytopathogenic fungi
Autor(a) principal: | |
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Data de Publicação: | 2022 |
Outros Autores: | , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Research, Society and Development |
Texto Completo: | https://rsdjournal.org/index.php/rsd/article/view/38445 |
Resumo: | The bees of the Meliponini tribe, known as stingless bees (SB), elaborate a distinct type of propolis, whose application potential is largely unexplored. Currently, there is some knowledge of the pharmacological activities of the propolis from Apis mellifera, however, little is known about the diversity and antimicrobial activity of Meliponini propolis, especially in relation to pathogens of agricultural importance. This study aimed to investigate the antimicrobial activity of the alcoholic extract of propolis from T. clavipes (Borá), S. bipunctata (Tubuna), and T. angustula (Jataí), comparing with the ethanolic extract from A. mellifera propolis. The autoclaved and non-autoclaved extracts were evaluated at the concentrations of zero, 1.6 %; 3.2 %; 6.4 %, and 12.0 % v/v. Fungal development was determined by measuring the mycelial diameter up to the fourteenth day of inoculation, with five replicates; the growth inhibition was calculated relative to the control. According to the results, the highest concentration of phenolic compounds and flavonoids was present in the ethanolic extract of A. mellifera propolis when compared to the SB extracts. The results indicated an inhibitory effect on the growth of the phytopathogens Colletotrichum gloeosporioides, Fusarium sp., Botryosphaeria sp., and Botrytis sp., mainly from 6.4 % and 12.0 % v/v when evaluated in the non-autoclaved medium, followed by the autoclaved medium. The fungus Botrytis sp. was the phytopathogen that suffered greater inhibition from 3.2 mL∙L-1, with the T. angustula propolis extract. The obtained results demonstrate that the alcoholic extracts of propolis from SB have the potential to control phytopathogenic fungi of agricultural interest. |
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Evaluation of the antifungal activity of propolis extracts from stingless bees on phytopathogenic fungiEvaluación de la actividad antifúngica de extractos de propóleos de abejas sin aguijón sobre hongos fitopatógenosAvaliação da atividade antifúngica de extratos de própolis de abelhas sem ferrão sobre fungos fitopatogênicosNatural antifungalsPhenolic compoundsTotal flavonoidsMeliponiniPropolis.MeliponiniAntifúngicos naturalesCompuestos fenólicosFlavonoides totalesPropóleos.MeliponiniAntifúngicos naturaisCompostos fenólicosFlavonoides totaisPrópolis.The bees of the Meliponini tribe, known as stingless bees (SB), elaborate a distinct type of propolis, whose application potential is largely unexplored. Currently, there is some knowledge of the pharmacological activities of the propolis from Apis mellifera, however, little is known about the diversity and antimicrobial activity of Meliponini propolis, especially in relation to pathogens of agricultural importance. This study aimed to investigate the antimicrobial activity of the alcoholic extract of propolis from T. clavipes (Borá), S. bipunctata (Tubuna), and T. angustula (Jataí), comparing with the ethanolic extract from A. mellifera propolis. The autoclaved and non-autoclaved extracts were evaluated at the concentrations of zero, 1.6 %; 3.2 %; 6.4 %, and 12.0 % v/v. Fungal development was determined by measuring the mycelial diameter up to the fourteenth day of inoculation, with five replicates; the growth inhibition was calculated relative to the control. According to the results, the highest concentration of phenolic compounds and flavonoids was present in the ethanolic extract of A. mellifera propolis when compared to the SB extracts. The results indicated an inhibitory effect on the growth of the phytopathogens Colletotrichum gloeosporioides, Fusarium sp., Botryosphaeria sp., and Botrytis sp., mainly from 6.4 % and 12.0 % v/v when evaluated in the non-autoclaved medium, followed by the autoclaved medium. The fungus Botrytis sp. was the phytopathogen that suffered greater inhibition from 3.2 mL∙L-1, with the T. angustula propolis extract. The obtained results demonstrate that the alcoholic extracts of propolis from SB have the potential to control phytopathogenic fungi of agricultural interest.Las abejas de la tribu Meliponini, conocidas como abejas sin aguijón (ASA), elaboran un tipo distinto de propóleos, cuyo potencial de aplicación está poco explorado. Actualmente, existe cierto conocimiento sobre las actividades farmacológicas del propóleo de Apis mellifera, sin embargo, poco se conoce sobre la diversidad y actividad antimicrobiana del propóleo de Meliponini, principalmente en relación con patógenos de importancia agrícola. El presente estudio tuvo como objetivo investigar la actividad antimicrobiana del extracto alcohólico de propóleo de T. clavipes (Borá), S. bipunctata (Tubuna) y T. angustula (Jataí), comparándolo con el extracto etanólico de propóleo de A. mellifera. Los extractos autoclavados y no autoclavados se evaluaron a concentraciones de cero, 1,6 %; 3,2 %; 6,4 % y 12,0 % v/v. El desarrollo fúngico se determinó midiendo el diámetro del micelio hasta el decimocuarto día de la inoculación, con cinco repeticiones; la inhibición del crecimiento se calculó en relación con el control. De acuerdo con los resultados, la mayor concentración de compuestos fenólicos y flavonoides se identificó en el extracto etanólico de propóleo de A. mellifera en comparación con los extractos de ASA. Los resultados del presente estudio confirmaron el efecto inhibitorio sobre el crecimiento de los fitopatógenos Colletotrichum gloeosporioides, Fusarium sp., Botryosphaeria sp. y Botrytis sp., principalmente a concentraciones de 6,4 % y 12,0 % v/v cuando se evaluó en medio no autoclavado, seguido de medio autoclavado. El hongo Botrytis sp. fue el fitopatógeno que sufrió mayor inhibición a partir de 3,2 mL∙L-1, con el extracto de propóleo de T. angustula. Los resultados obtenidos demuestran que los extractos alcohólicos de propóleo ASF tienen potencial para el control de hongos fitopatógenos de interés agrícola.As abelhas da tribo Meliponini, conhecidas como abelhas sem ferrão (ASF), elaboram um tipo distinto de própolis, cujo potencial de aplicação é pouco explorado. Atualmente, existe algum conhecimento sobre as atividades farmacológicas da própolis de Apis mellifera, entretanto, pouco se sabe sobre a diversidade e atividade antimicrobiana da própolis de Meliponini, principalmente em relação a patógenos de importância agrícola. Este estudo teve como objetivo investigar a atividade antimicrobiana do extrato alcoólico da própolis de T. clavipes (Borá), S. bipunctata (Tubuna) e T. angustula (Jataí), comparando com o extrato etanólico da própolis de A. mellifera. Os extratos autoclavados e não autoclavados foram avaliados nas concentrações de zero, 1,6 %; 3,2 %; 6,4 % e 12,0 % v/v. O desenvolvimento fúngico foi determinado medindo-se o diâmetro micelial até o décimo quarto dia de inoculação, com cinco repetições; a inibição do crescimento foi calculada em relação ao controle. De acordo com os resultados, a maior concentração de compostos fenólicos e flavonoides foi identificada no extrato etanólico da própolis de A. mellifera quando comparado aos extratos de ASF. Os resultados do presente estudo confirmaram o efeito inibitório sobre o crescimento dos fitopatógenos Colletotrichum gloeosporioides, Fusarium sp., Botryosphaeria sp. e Botrytis sp., principalmente a partir de 6,4 % e 12,0 % v/v quando avaliados em meio não autoclavado, seguido do meio autoclavado. O fungo Botrytis sp. foi o fitopatógeno que sofreu maior inibição a partir de 3,2 mL∙L-1, com o extrato de própolis de T. angustula. Os resultados obtidos demonstram que os extratos alcoólicos de própolis de ASF têm potencial para controlar fungos fitopatogênicos de interesse agrícola.Research, Society and Development2022-12-14info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://rsdjournal.org/index.php/rsd/article/view/3844510.33448/rsd-v11i16.38445Research, Society and Development; Vol. 11 No. 16; e445111638445Research, Society and Development; Vol. 11 Núm. 16; e445111638445Research, Society and Development; v. 11 n. 16; e4451116384452525-3409reponame:Research, Society and Developmentinstname:Universidade Federal de Itajubá (UNIFEI)instacron:UNIFEIenghttps://rsdjournal.org/index.php/rsd/article/view/38445/31820Copyright (c) 2022 Clarice Rech Costantin; Helena Gil de Oliveira; Luiza San Martins Roese; Márcia Regina Pancera; Wendel Paulo Silvestre; Gustavo Rubbo Siqueira; Nadilson Roberto Ferreira; Valdirene Camatti Sartorihttps://creativecommons.org/licenses/by/4.0info:eu-repo/semantics/openAccessCostantin, Clarice Rech Oliveira, Helena Gil de Roese, Luiza San Martins Pancera, Márcia ReginaSilvestre, Wendel PauloSiqueira, Gustavo Rubbo Ferreira, Nadilson RobertoSartori, Valdirene Camatti 2022-12-18T18:26:42Zoai:ojs.pkp.sfu.ca:article/38445Revistahttps://rsdjournal.org/index.php/rsd/indexPUBhttps://rsdjournal.org/index.php/rsd/oairsd.articles@gmail.com2525-34092525-3409opendoar:2024-01-17T09:52:11.242365Research, Society and Development - Universidade Federal de Itajubá (UNIFEI)false |
dc.title.none.fl_str_mv |
Evaluation of the antifungal activity of propolis extracts from stingless bees on phytopathogenic fungi Evaluación de la actividad antifúngica de extractos de propóleos de abejas sin aguijón sobre hongos fitopatógenos Avaliação da atividade antifúngica de extratos de própolis de abelhas sem ferrão sobre fungos fitopatogênicos |
title |
Evaluation of the antifungal activity of propolis extracts from stingless bees on phytopathogenic fungi |
spellingShingle |
Evaluation of the antifungal activity of propolis extracts from stingless bees on phytopathogenic fungi Costantin, Clarice Rech Natural antifungals Phenolic compounds Total flavonoids Meliponini Propolis. Meliponini Antifúngicos naturales Compuestos fenólicos Flavonoides totales Propóleos. Meliponini Antifúngicos naturais Compostos fenólicos Flavonoides totais Própolis. |
title_short |
Evaluation of the antifungal activity of propolis extracts from stingless bees on phytopathogenic fungi |
title_full |
Evaluation of the antifungal activity of propolis extracts from stingless bees on phytopathogenic fungi |
title_fullStr |
Evaluation of the antifungal activity of propolis extracts from stingless bees on phytopathogenic fungi |
title_full_unstemmed |
Evaluation of the antifungal activity of propolis extracts from stingless bees on phytopathogenic fungi |
title_sort |
Evaluation of the antifungal activity of propolis extracts from stingless bees on phytopathogenic fungi |
author |
Costantin, Clarice Rech |
author_facet |
Costantin, Clarice Rech Oliveira, Helena Gil de Roese, Luiza San Martins Pancera, Márcia Regina Silvestre, Wendel Paulo Siqueira, Gustavo Rubbo Ferreira, Nadilson Roberto Sartori, Valdirene Camatti |
author_role |
author |
author2 |
Oliveira, Helena Gil de Roese, Luiza San Martins Pancera, Márcia Regina Silvestre, Wendel Paulo Siqueira, Gustavo Rubbo Ferreira, Nadilson Roberto Sartori, Valdirene Camatti |
author2_role |
author author author author author author author |
dc.contributor.author.fl_str_mv |
Costantin, Clarice Rech Oliveira, Helena Gil de Roese, Luiza San Martins Pancera, Márcia Regina Silvestre, Wendel Paulo Siqueira, Gustavo Rubbo Ferreira, Nadilson Roberto Sartori, Valdirene Camatti |
dc.subject.por.fl_str_mv |
Natural antifungals Phenolic compounds Total flavonoids Meliponini Propolis. Meliponini Antifúngicos naturales Compuestos fenólicos Flavonoides totales Propóleos. Meliponini Antifúngicos naturais Compostos fenólicos Flavonoides totais Própolis. |
topic |
Natural antifungals Phenolic compounds Total flavonoids Meliponini Propolis. Meliponini Antifúngicos naturales Compuestos fenólicos Flavonoides totales Propóleos. Meliponini Antifúngicos naturais Compostos fenólicos Flavonoides totais Própolis. |
description |
The bees of the Meliponini tribe, known as stingless bees (SB), elaborate a distinct type of propolis, whose application potential is largely unexplored. Currently, there is some knowledge of the pharmacological activities of the propolis from Apis mellifera, however, little is known about the diversity and antimicrobial activity of Meliponini propolis, especially in relation to pathogens of agricultural importance. This study aimed to investigate the antimicrobial activity of the alcoholic extract of propolis from T. clavipes (Borá), S. bipunctata (Tubuna), and T. angustula (Jataí), comparing with the ethanolic extract from A. mellifera propolis. The autoclaved and non-autoclaved extracts were evaluated at the concentrations of zero, 1.6 %; 3.2 %; 6.4 %, and 12.0 % v/v. Fungal development was determined by measuring the mycelial diameter up to the fourteenth day of inoculation, with five replicates; the growth inhibition was calculated relative to the control. According to the results, the highest concentration of phenolic compounds and flavonoids was present in the ethanolic extract of A. mellifera propolis when compared to the SB extracts. The results indicated an inhibitory effect on the growth of the phytopathogens Colletotrichum gloeosporioides, Fusarium sp., Botryosphaeria sp., and Botrytis sp., mainly from 6.4 % and 12.0 % v/v when evaluated in the non-autoclaved medium, followed by the autoclaved medium. The fungus Botrytis sp. was the phytopathogen that suffered greater inhibition from 3.2 mL∙L-1, with the T. angustula propolis extract. The obtained results demonstrate that the alcoholic extracts of propolis from SB have the potential to control phytopathogenic fungi of agricultural interest. |
publishDate |
2022 |
dc.date.none.fl_str_mv |
2022-12-14 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
https://rsdjournal.org/index.php/rsd/article/view/38445 10.33448/rsd-v11i16.38445 |
url |
https://rsdjournal.org/index.php/rsd/article/view/38445 |
identifier_str_mv |
10.33448/rsd-v11i16.38445 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
https://rsdjournal.org/index.php/rsd/article/view/38445/31820 |
dc.rights.driver.fl_str_mv |
https://creativecommons.org/licenses/by/4.0 info:eu-repo/semantics/openAccess |
rights_invalid_str_mv |
https://creativecommons.org/licenses/by/4.0 |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Research, Society and Development |
publisher.none.fl_str_mv |
Research, Society and Development |
dc.source.none.fl_str_mv |
Research, Society and Development; Vol. 11 No. 16; e445111638445 Research, Society and Development; Vol. 11 Núm. 16; e445111638445 Research, Society and Development; v. 11 n. 16; e445111638445 2525-3409 reponame:Research, Society and Development instname:Universidade Federal de Itajubá (UNIFEI) instacron:UNIFEI |
instname_str |
Universidade Federal de Itajubá (UNIFEI) |
instacron_str |
UNIFEI |
institution |
UNIFEI |
reponame_str |
Research, Society and Development |
collection |
Research, Society and Development |
repository.name.fl_str_mv |
Research, Society and Development - Universidade Federal de Itajubá (UNIFEI) |
repository.mail.fl_str_mv |
rsd.articles@gmail.com |
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1797052828116582400 |