Brazilian green propolis doesn't have a beneficial effect on the cryopreservation of domestic cat epididymal spermatozoa
Autor(a) principal: | |
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Data de Publicação: | 2021 |
Outros Autores: | , , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Research, Society and Development |
Texto Completo: | https://rsdjournal.org/index.php/rsd/article/view/12842 |
Resumo: | This study evaluated the effect of different concentrations of Brazilian green propolis on the cryopreservation of epididymal sperm from domestic cats. Spermatozoa were collected from cauda epididymis by slicing technique in a TRIS extender, preheated to 37ºC. Then, the sperm were evaluated and divided randomly between the control group (without adding propolis in the extender) and the treatment groups (adding different concentrations of propolis extract in the extender): P1 (0.1 mg/mL), P2 (0.3 mg/mL) e P3 (0.6 mg/mL). Subsequently, sperm were cryopreserved. The evaluated parameters were sperm kinetics by the Computer Assisted Semen Analysis, vigor, viability, membrane functionality, chromatin condensation and morphology. The parameters were measured before and after cryopreservation. Propolis didn’t show toxicity to the sperm in any concentrations, with no changes observed in the motility pattern. There was no significant influence of propolis on motility, vigor, percentage of viable spermatozoa, chromatin quality and other kinetic parameters in the cryopreserved samples. In sperm morphology, it was demonstrated a reduction in the percentage of normal cells in P1 and P2 groups in relation to the fresh sample, control and P3 group. In addition, an interesting effect from the propolis groups was observed on possible growth inhibition of microorganisms in the contaminated samples. Propolis didn’t provide superiority to the sperm parameters evaluated after thawing, except for the values of plasma membrane functionality, which were better. The propolis is an interesting component to be incorporated in the cryoprotectant medium due to its non-toxicity and the potential inhibition of microbial growth. |
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Brazilian green propolis doesn't have a beneficial effect on the cryopreservation of domestic cat epididymal spermatozoaEl propóleo verde brasileño no tiene un efecto beneficioso sobre la criopreservación de espermatozoides epididimarios de gatos domésticosA própolis verde brasileira não tem efeito benéfico na criopreservação de espermatozóides epididimários de gato doméstico FelinoBiotecnologías reproductivasEspermogramaCASAPropóleo verde.FelineReproductive biotechiniquesSpermogramCASAGreen propolis.FelinosBiotecnologias reprodutivasEspermogramaCASAPropolis verde.This study evaluated the effect of different concentrations of Brazilian green propolis on the cryopreservation of epididymal sperm from domestic cats. Spermatozoa were collected from cauda epididymis by slicing technique in a TRIS extender, preheated to 37ºC. Then, the sperm were evaluated and divided randomly between the control group (without adding propolis in the extender) and the treatment groups (adding different concentrations of propolis extract in the extender): P1 (0.1 mg/mL), P2 (0.3 mg/mL) e P3 (0.6 mg/mL). Subsequently, sperm were cryopreserved. The evaluated parameters were sperm kinetics by the Computer Assisted Semen Analysis, vigor, viability, membrane functionality, chromatin condensation and morphology. The parameters were measured before and after cryopreservation. Propolis didn’t show toxicity to the sperm in any concentrations, with no changes observed in the motility pattern. There was no significant influence of propolis on motility, vigor, percentage of viable spermatozoa, chromatin quality and other kinetic parameters in the cryopreserved samples. In sperm morphology, it was demonstrated a reduction in the percentage of normal cells in P1 and P2 groups in relation to the fresh sample, control and P3 group. In addition, an interesting effect from the propolis groups was observed on possible growth inhibition of microorganisms in the contaminated samples. Propolis didn’t provide superiority to the sperm parameters evaluated after thawing, except for the values of plasma membrane functionality, which were better. The propolis is an interesting component to be incorporated in the cryoprotectant medium due to its non-toxicity and the potential inhibition of microbial growth.Este estudio evaluó el efecto de diferentes concentraciones de propóleo verde brasileño en la criopreservación de esperma epididimario de gatos domésticos. Los espermatozoides se recolectaron de la cola del epidídimo mediante la técnica de corte, en un extensor TRIS, precalentado a 37ºC. Luego, los espermatozoides fueron evaluados y divididos aleatoriamente entre el grupo de control (sin agregar propóleo en el diluyente) y grupos de tratamiento (adición de diferentes concentraciones de extracto de propóleo en el diluyente): P1 (0,1 mg/mL), P2 (0,3 mg/mL) y P3 (0,6 mg/mL). Posteriormente, los espermatozoides fueron criopreservados. Los parámetros evaluados fueron: cinética espermática por análisis de semen asistido por computadora, vigor, viabilidad, funcionalidad de membrana, condensación de cromatina y morfología. Los parámetros se midieron antes y después de la criopreservación. El propóleo no mostró toxicidad para los espermatozoides a ninguna concentración, no se observó ningún cambio no estándar en la motilidad. No hubo influencia significativa del propóleo sobre la motilidad, vigor, porcentaje de espermatozoides viables, calidad de cromatina y otros parámetros cinéticos en muestras criopreservadas. En la morfología de los espermatozoides, se demostró una reducción del porcentaje de células normales en los grupos P1 y P2 en relación con la muestra fresca, el control y el grupo P3. Además, se observó un interesante efecto de los grupos propóleos sobre la posible inhibición del crecimiento de microorganismos en las muestras contaminadas. El propóleo no mostró superioridad a los parámetros espermáticos evaluados después de la descongelación, excepto por los valores de funcionalidad de la membrana plasmática, que fueron mejores. El propóleo es un componente interesante para ser incorporado al medio crioprotector por su no toxicidad y potencial para inhibir el crecimiento microbiano.Este estudo avaliou o efeito de diferentes concentrações da própolis verde brasileira na criopreservação dos espermatozoides epididimários de gatos domésticos. Os espermatozóides foram coletados da cauda do epidídimo pela técnica de fatiamento, em extensor TRIS, pré-aquecido a 37ºC. Em seguida, os espermatozoides foram avaliados e divididos aleatoriamente entre o grupo controle (sem adição de própolis no diluente) e os grupos de tratamento (adição de diferentes concentrações de extrato de própolis no diluente): P1 (0,1 mg/mL), P2 (0,3 mg/mL) e P3 (0,6 mg/mL). Posteriormente, os espermatozoides foram criopreservados. Os parâmetros avaliados foram: cinética espermática pela Análise de Sêmen Assistida por Computador, vigor, viabilidade, funcionalidade da membrana, condensação da cromatina e morfologia. Os parâmetros foram medidos antes e pós-criopreservação. A própolis não apresentou toxicidade aos espermatozoides em nenhuma concentração, não sendo observada alteração no padrão de motilidade. Não houve influência significativa da própolis na motilidade, vigor, porcentagem de espermatozóides viáveis, qualidade da cromatina e outros parâmetros cinéticos nas amostras criopreservadas. Na morfologia espermática, foi demonstrada redução no percentual de células normais nos grupos P1 e P2 em relação à amostra fresca, controle e grupo P3. Além disso, foi observado um efeito interessante dos grupos de própolis sobre a possível inibição do crescimento de microrganismos nas amostras contaminadas. A própolis não apresentou superioridade aos parâmetros espermáticos avaliados pós-descongelação, exceto para os valores de funcionalidade da membrana plasmática, que foram melhores. A própolis é um componente interessante de ser incorporado ao meio crioprotetor devido à sua não toxicidade e potencial inibidor do crescimento microbiano.Research, Society and Development2021-02-25info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://rsdjournal.org/index.php/rsd/article/view/1284210.33448/rsd-v10i2.12842Research, Society and Development; Vol. 10 No. 2; e48710212842Research, Society and Development; Vol. 10 Núm. 2; e48710212842Research, Society and Development; v. 10 n. 2; e487102128422525-3409reponame:Research, Society and Developmentinstname:Universidade Federal de Itajubá (UNIFEI)instacron:UNIFEIenghttps://rsdjournal.org/index.php/rsd/article/view/12842/11517Copyright (c) 2021 Henrique Albano Nogueira Gomes; Denilsa Pires Fernandes ; Brenna de Sousa Barbosa; Roberta Gonçalves Izzo; Fernanda Araujo dos Santos; Andréia Maria da Silva; Luã Barbalho de Macêdo; Parmênedes Dias de Brito; Alexandre Rodrigues Silva; Marcelo Barbosa Bezerrahttps://creativecommons.org/licenses/by/4.0info:eu-repo/semantics/openAccessGomes, Henrique Albano Nogueira Fernandes , Denilsa Pires Barbosa, Brenna de Sousa Izzo, Roberta Gonçalves Santos, Fernanda Araujo dos Silva, Andréia Maria da Macêdo, Luã Barbalho de Brito, Parmênedes Dias de Silva, Alexandre Rodrigues Bezerra, Marcelo Barbosa 2021-03-02T09:32:39Zoai:ojs.pkp.sfu.ca:article/12842Revistahttps://rsdjournal.org/index.php/rsd/indexPUBhttps://rsdjournal.org/index.php/rsd/oairsd.articles@gmail.com2525-34092525-3409opendoar:2024-01-17T09:34:19.028564Research, Society and Development - Universidade Federal de Itajubá (UNIFEI)false |
dc.title.none.fl_str_mv |
Brazilian green propolis doesn't have a beneficial effect on the cryopreservation of domestic cat epididymal spermatozoa El propóleo verde brasileño no tiene un efecto beneficioso sobre la criopreservación de espermatozoides epididimarios de gatos domésticos A própolis verde brasileira não tem efeito benéfico na criopreservação de espermatozóides epididimários de gato doméstico |
title |
Brazilian green propolis doesn't have a beneficial effect on the cryopreservation of domestic cat epididymal spermatozoa |
spellingShingle |
Brazilian green propolis doesn't have a beneficial effect on the cryopreservation of domestic cat epididymal spermatozoa Gomes, Henrique Albano Nogueira Felino Biotecnologías reproductivas Espermograma CASA Propóleo verde. Feline Reproductive biotechiniques Spermogram CASA Green propolis. Felinos Biotecnologias reprodutivas Espermograma CASA Propolis verde. |
title_short |
Brazilian green propolis doesn't have a beneficial effect on the cryopreservation of domestic cat epididymal spermatozoa |
title_full |
Brazilian green propolis doesn't have a beneficial effect on the cryopreservation of domestic cat epididymal spermatozoa |
title_fullStr |
Brazilian green propolis doesn't have a beneficial effect on the cryopreservation of domestic cat epididymal spermatozoa |
title_full_unstemmed |
Brazilian green propolis doesn't have a beneficial effect on the cryopreservation of domestic cat epididymal spermatozoa |
title_sort |
Brazilian green propolis doesn't have a beneficial effect on the cryopreservation of domestic cat epididymal spermatozoa |
author |
Gomes, Henrique Albano Nogueira |
author_facet |
Gomes, Henrique Albano Nogueira Fernandes , Denilsa Pires Barbosa, Brenna de Sousa Izzo, Roberta Gonçalves Santos, Fernanda Araujo dos Silva, Andréia Maria da Macêdo, Luã Barbalho de Brito, Parmênedes Dias de Silva, Alexandre Rodrigues Bezerra, Marcelo Barbosa |
author_role |
author |
author2 |
Fernandes , Denilsa Pires Barbosa, Brenna de Sousa Izzo, Roberta Gonçalves Santos, Fernanda Araujo dos Silva, Andréia Maria da Macêdo, Luã Barbalho de Brito, Parmênedes Dias de Silva, Alexandre Rodrigues Bezerra, Marcelo Barbosa |
author2_role |
author author author author author author author author author |
dc.contributor.author.fl_str_mv |
Gomes, Henrique Albano Nogueira Fernandes , Denilsa Pires Barbosa, Brenna de Sousa Izzo, Roberta Gonçalves Santos, Fernanda Araujo dos Silva, Andréia Maria da Macêdo, Luã Barbalho de Brito, Parmênedes Dias de Silva, Alexandre Rodrigues Bezerra, Marcelo Barbosa |
dc.subject.por.fl_str_mv |
Felino Biotecnologías reproductivas Espermograma CASA Propóleo verde. Feline Reproductive biotechiniques Spermogram CASA Green propolis. Felinos Biotecnologias reprodutivas Espermograma CASA Propolis verde. |
topic |
Felino Biotecnologías reproductivas Espermograma CASA Propóleo verde. Feline Reproductive biotechiniques Spermogram CASA Green propolis. Felinos Biotecnologias reprodutivas Espermograma CASA Propolis verde. |
description |
This study evaluated the effect of different concentrations of Brazilian green propolis on the cryopreservation of epididymal sperm from domestic cats. Spermatozoa were collected from cauda epididymis by slicing technique in a TRIS extender, preheated to 37ºC. Then, the sperm were evaluated and divided randomly between the control group (without adding propolis in the extender) and the treatment groups (adding different concentrations of propolis extract in the extender): P1 (0.1 mg/mL), P2 (0.3 mg/mL) e P3 (0.6 mg/mL). Subsequently, sperm were cryopreserved. The evaluated parameters were sperm kinetics by the Computer Assisted Semen Analysis, vigor, viability, membrane functionality, chromatin condensation and morphology. The parameters were measured before and after cryopreservation. Propolis didn’t show toxicity to the sperm in any concentrations, with no changes observed in the motility pattern. There was no significant influence of propolis on motility, vigor, percentage of viable spermatozoa, chromatin quality and other kinetic parameters in the cryopreserved samples. In sperm morphology, it was demonstrated a reduction in the percentage of normal cells in P1 and P2 groups in relation to the fresh sample, control and P3 group. In addition, an interesting effect from the propolis groups was observed on possible growth inhibition of microorganisms in the contaminated samples. Propolis didn’t provide superiority to the sperm parameters evaluated after thawing, except for the values of plasma membrane functionality, which were better. The propolis is an interesting component to be incorporated in the cryoprotectant medium due to its non-toxicity and the potential inhibition of microbial growth. |
publishDate |
2021 |
dc.date.none.fl_str_mv |
2021-02-25 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
https://rsdjournal.org/index.php/rsd/article/view/12842 10.33448/rsd-v10i2.12842 |
url |
https://rsdjournal.org/index.php/rsd/article/view/12842 |
identifier_str_mv |
10.33448/rsd-v10i2.12842 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
https://rsdjournal.org/index.php/rsd/article/view/12842/11517 |
dc.rights.driver.fl_str_mv |
https://creativecommons.org/licenses/by/4.0 info:eu-repo/semantics/openAccess |
rights_invalid_str_mv |
https://creativecommons.org/licenses/by/4.0 |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Research, Society and Development |
publisher.none.fl_str_mv |
Research, Society and Development |
dc.source.none.fl_str_mv |
Research, Society and Development; Vol. 10 No. 2; e48710212842 Research, Society and Development; Vol. 10 Núm. 2; e48710212842 Research, Society and Development; v. 10 n. 2; e48710212842 2525-3409 reponame:Research, Society and Development instname:Universidade Federal de Itajubá (UNIFEI) instacron:UNIFEI |
instname_str |
Universidade Federal de Itajubá (UNIFEI) |
instacron_str |
UNIFEI |
institution |
UNIFEI |
reponame_str |
Research, Society and Development |
collection |
Research, Society and Development |
repository.name.fl_str_mv |
Research, Society and Development - Universidade Federal de Itajubá (UNIFEI) |
repository.mail.fl_str_mv |
rsd.articles@gmail.com |
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1797052746198679552 |