Immunohistochemical and histomorphometric analysis of the endometrium of rats in permanent estrus treated with melatonin, metformin and clomiphene citrate

Detalhes bibliográficos
Autor(a) principal: Mattos, Leandro Sabará de
Data de Publicação: 2022
Outros Autores: Lombardi , Leonardo Augusto, Moura, Marcio Luis Alves, Espindula, Ana Paula, Simões, Ricardo Santos, Florencio-Silva, Rinaldo, Franco, Paulo Celso, Sasso, Gisela Rodrigues da Silva, Simões, Manuel de Jesus
Tipo de documento: Artigo
Idioma: por
Título da fonte: Research, Society and Development
Texto Completo: https://rsdjournal.org/index.php/rsd/article/view/29995
Resumo: Objective: To evaluate the effects of melatonin, metformin and clomiphene citrate on the endometrium of polycystic ovary syndrome model rats. Methods: Seventy-eight rats were divided into 13 groups and two experiments (prevention and treatment). After exposure, all animals were anesthetized and the uterine horns were removed and submitted to hematoxylin and eosin staining and immunohistochemistry (Ki-67 and Vegf). The data obtained were submitted to Student's t test (p<0.05). Results/Conclusion: Melatonin, metformin and clomiphene citrate do not promote proliferative and angiogenic effects on the endometrium of rats in permanent estrus, however, melatonin has a more significant trophic effect on the endometrial stroma, which did not interfere with its thickness, while that metformin had a more significant trophic effect on endometrial glandular epithelium, therefore, these effects are similar both before and after PCOS induction by continuous light exposure.
id UNIFEI_88f81ec20f19138b99ee5b69b8a28fe3
oai_identifier_str oai:ojs.pkp.sfu.ca:article/29995
network_acronym_str UNIFEI
network_name_str Research, Society and Development
repository_id_str
spelling Immunohistochemical and histomorphometric analysis of the endometrium of rats in permanent estrus treated with melatonin, metformin and clomiphene citrateAnálisis inmunohistoquímico e histomorfométrico del endometrio de ratas en celo permanente tratadas con melatonina, metformina y citrato de clomifenoAnálise imunoistoquímica e histomorfométrica do endométrio de ratas em estro permanente tratadas com melatonina, metformina e citrato de clomifenoEndométrioEstro permanenteEnsino em SaúdeMelatoninaMetforminaCitrato de clomifenoRatas.EndometrioEstro permanenteEnseñanza en saludMelatoninaMetforminaCitrato de clomifenoRatas.EndometriumPermanent estrusHealth teachingMelatoninMetforminClomiphene citrateRats.Objective: To evaluate the effects of melatonin, metformin and clomiphene citrate on the endometrium of polycystic ovary syndrome model rats. Methods: Seventy-eight rats were divided into 13 groups and two experiments (prevention and treatment). After exposure, all animals were anesthetized and the uterine horns were removed and submitted to hematoxylin and eosin staining and immunohistochemistry (Ki-67 and Vegf). The data obtained were submitted to Student's t test (p<0.05). Results/Conclusion: Melatonin, metformin and clomiphene citrate do not promote proliferative and angiogenic effects on the endometrium of rats in permanent estrus, however, melatonin has a more significant trophic effect on the endometrial stroma, which did not interfere with its thickness, while that metformin had a more significant trophic effect on endometrial glandular epithelium, therefore, these effects are similar both before and after PCOS induction by continuous light exposure.Objetivo: Evaluar los efectos de la melatonina, metformina y citrato de clomifeno en el endometrio de ratas modelo con síndrome de ovario poliquístico. Métodos: Setenta y ocho ratas se dividieron en 13 grupos y dos experimentos (prevención y tratamiento). Después de la exposición, todos los animales fueron anestesiados y los cuernos uterinos fueron removidos y sometidos a tinción con hematoxilina y eosina e inmunohistoquímica (Ki-67 y Vegf). Los datos obtenidos se sometieron a la prueba de la t de Student (p<0,05). Resultados/Conclusión: La melatonina, la metformina y el citrato de clomifeno no promueven efectos proliferativos y angiogénicos sobre el endometrio de ratas en celo permanente, sin embargo, la melatonina tiene un efecto trófico más significativo sobre el estroma endometrial, que no interfiere con su espesor, mientras que la metformina tuvo un efecto trófico más significativo sobre el epitelio glandular endometrial, por lo tanto, estos efectos son similares tanto antes como después de la inducción del SOP por exposición continua a la luz.Objetivo: Avaliar os efeitos da melatonina, metformina e citrato de clomifeno no endométrio de ratas modelo de síndrome dos ovários policísticos. Métodos: Setenta e oito ratas foram divididas em 13 grupos e dois experimentos (prevenção e tratamento). Após as exposições todos os animais foram anestesiados e tiveram retirados os cornos uterinos e submetidos a coloração pela hematoxilina e eosina e imunoistoquímica (Ki-67 e Vegf). Os dados obtidos foram submetidos ao teste t de Student (p<0,05). Resultados/Conclusão: A melatonina, a metformina e o citrato de clomifeno não promovem efeitos proliferativos e angiogênicos no endométrio de ratas em estro permanente, porém, a melatonina tem efeito trófico mais significativo no estroma endometrial, o que não interferiu na sua espessura, enquanto que a metformina apresentou efeito trófico mais significante no epitélio glandular do endométrio, portanto, estes efeitos são semelhantes tanto antes quanto após a indução de SOP por exposição à luz contínua.Research, Society and Development2022-05-24info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://rsdjournal.org/index.php/rsd/article/view/2999510.33448/rsd-v11i7.29995Research, Society and Development; Vol. 11 No. 7; e28011729995Research, Society and Development; Vol. 11 Núm. 7; e28011729995Research, Society and Development; v. 11 n. 7; e280117299952525-3409reponame:Research, Society and Developmentinstname:Universidade Federal de Itajubá (UNIFEI)instacron:UNIFEIporhttps://rsdjournal.org/index.php/rsd/article/view/29995/25865Copyright (c) 2022 Leandro Sabará de Mattos; Leonardo Augusto Lombardi ; Marcio Luis Alves Moura; Ana Paula Espindula; Ricardo Santos Simões; Rinaldo Florencio-Silva; Paulo Celso Franco; Gisela Rodrigues da Silva Sasso; Manuel de Jesus Simõeshttps://creativecommons.org/licenses/by/4.0info:eu-repo/semantics/openAccessMattos, Leandro Sabará deLombardi , Leonardo Augusto Moura, Marcio Luis Alves Espindula, Ana PaulaSimões, Ricardo Santos Florencio-Silva, Rinaldo Franco, Paulo Celso Sasso, Gisela Rodrigues da Silva Simões, Manuel de Jesus 2022-06-06T15:12:05Zoai:ojs.pkp.sfu.ca:article/29995Revistahttps://rsdjournal.org/index.php/rsd/indexPUBhttps://rsdjournal.org/index.php/rsd/oairsd.articles@gmail.com2525-34092525-3409opendoar:2024-01-17T09:46:54.540064Research, Society and Development - Universidade Federal de Itajubá (UNIFEI)false
dc.title.none.fl_str_mv Immunohistochemical and histomorphometric analysis of the endometrium of rats in permanent estrus treated with melatonin, metformin and clomiphene citrate
Análisis inmunohistoquímico e histomorfométrico del endometrio de ratas en celo permanente tratadas con melatonina, metformina y citrato de clomifeno
Análise imunoistoquímica e histomorfométrica do endométrio de ratas em estro permanente tratadas com melatonina, metformina e citrato de clomifeno
title Immunohistochemical and histomorphometric analysis of the endometrium of rats in permanent estrus treated with melatonin, metformin and clomiphene citrate
spellingShingle Immunohistochemical and histomorphometric analysis of the endometrium of rats in permanent estrus treated with melatonin, metformin and clomiphene citrate
Mattos, Leandro Sabará de
Endométrio
Estro permanente
Ensino em Saúde
Melatonina
Metformina
Citrato de clomifeno
Ratas.
Endometrio
Estro permanente
Enseñanza en salud
Melatonina
Metformina
Citrato de clomifeno
Ratas.
Endometrium
Permanent estrus
Health teaching
Melatonin
Metformin
Clomiphene citrate
Rats.
title_short Immunohistochemical and histomorphometric analysis of the endometrium of rats in permanent estrus treated with melatonin, metformin and clomiphene citrate
title_full Immunohistochemical and histomorphometric analysis of the endometrium of rats in permanent estrus treated with melatonin, metformin and clomiphene citrate
title_fullStr Immunohistochemical and histomorphometric analysis of the endometrium of rats in permanent estrus treated with melatonin, metformin and clomiphene citrate
title_full_unstemmed Immunohistochemical and histomorphometric analysis of the endometrium of rats in permanent estrus treated with melatonin, metformin and clomiphene citrate
title_sort Immunohistochemical and histomorphometric analysis of the endometrium of rats in permanent estrus treated with melatonin, metformin and clomiphene citrate
author Mattos, Leandro Sabará de
author_facet Mattos, Leandro Sabará de
Lombardi , Leonardo Augusto
Moura, Marcio Luis Alves
Espindula, Ana Paula
Simões, Ricardo Santos
Florencio-Silva, Rinaldo
Franco, Paulo Celso
Sasso, Gisela Rodrigues da Silva
Simões, Manuel de Jesus
author_role author
author2 Lombardi , Leonardo Augusto
Moura, Marcio Luis Alves
Espindula, Ana Paula
Simões, Ricardo Santos
Florencio-Silva, Rinaldo
Franco, Paulo Celso
Sasso, Gisela Rodrigues da Silva
Simões, Manuel de Jesus
author2_role author
author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv Mattos, Leandro Sabará de
Lombardi , Leonardo Augusto
Moura, Marcio Luis Alves
Espindula, Ana Paula
Simões, Ricardo Santos
Florencio-Silva, Rinaldo
Franco, Paulo Celso
Sasso, Gisela Rodrigues da Silva
Simões, Manuel de Jesus
dc.subject.por.fl_str_mv Endométrio
Estro permanente
Ensino em Saúde
Melatonina
Metformina
Citrato de clomifeno
Ratas.
Endometrio
Estro permanente
Enseñanza en salud
Melatonina
Metformina
Citrato de clomifeno
Ratas.
Endometrium
Permanent estrus
Health teaching
Melatonin
Metformin
Clomiphene citrate
Rats.
topic Endométrio
Estro permanente
Ensino em Saúde
Melatonina
Metformina
Citrato de clomifeno
Ratas.
Endometrio
Estro permanente
Enseñanza en salud
Melatonina
Metformina
Citrato de clomifeno
Ratas.
Endometrium
Permanent estrus
Health teaching
Melatonin
Metformin
Clomiphene citrate
Rats.
description Objective: To evaluate the effects of melatonin, metformin and clomiphene citrate on the endometrium of polycystic ovary syndrome model rats. Methods: Seventy-eight rats were divided into 13 groups and two experiments (prevention and treatment). After exposure, all animals were anesthetized and the uterine horns were removed and submitted to hematoxylin and eosin staining and immunohistochemistry (Ki-67 and Vegf). The data obtained were submitted to Student's t test (p<0.05). Results/Conclusion: Melatonin, metformin and clomiphene citrate do not promote proliferative and angiogenic effects on the endometrium of rats in permanent estrus, however, melatonin has a more significant trophic effect on the endometrial stroma, which did not interfere with its thickness, while that metformin had a more significant trophic effect on endometrial glandular epithelium, therefore, these effects are similar both before and after PCOS induction by continuous light exposure.
publishDate 2022
dc.date.none.fl_str_mv 2022-05-24
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv https://rsdjournal.org/index.php/rsd/article/view/29995
10.33448/rsd-v11i7.29995
url https://rsdjournal.org/index.php/rsd/article/view/29995
identifier_str_mv 10.33448/rsd-v11i7.29995
dc.language.iso.fl_str_mv por
language por
dc.relation.none.fl_str_mv https://rsdjournal.org/index.php/rsd/article/view/29995/25865
dc.rights.driver.fl_str_mv https://creativecommons.org/licenses/by/4.0
info:eu-repo/semantics/openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by/4.0
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Research, Society and Development
publisher.none.fl_str_mv Research, Society and Development
dc.source.none.fl_str_mv Research, Society and Development; Vol. 11 No. 7; e28011729995
Research, Society and Development; Vol. 11 Núm. 7; e28011729995
Research, Society and Development; v. 11 n. 7; e28011729995
2525-3409
reponame:Research, Society and Development
instname:Universidade Federal de Itajubá (UNIFEI)
instacron:UNIFEI
instname_str Universidade Federal de Itajubá (UNIFEI)
instacron_str UNIFEI
institution UNIFEI
reponame_str Research, Society and Development
collection Research, Society and Development
repository.name.fl_str_mv Research, Society and Development - Universidade Federal de Itajubá (UNIFEI)
repository.mail.fl_str_mv rsd.articles@gmail.com
_version_ 1797052767008718848