Inhibitory and bactericidal activities of Lippia origanoides essential oil against Escherichia coli, Klebsiella pneumoniae and Pseudomonas aeruginosa multidrug resistant
Autor(a) principal: | |
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Data de Publicação: | 2022 |
Outros Autores: | , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Research, Society and Development |
Texto Completo: | https://rsdjournal.org/index.php/rsd/article/view/31478 |
Resumo: | The objective of this study was to evaluate the antimicrobial potential of essential oil (EO) from L. origanoides against Gram-negative bacteria with multidrug resistance (MDR) phenotype and to correlate the results obtained with the genetic profile of these microorganisms. The oil was extracted from the leaves of this plant by steam dragging and condensation with Clevenger equipment, the chemical components were identified and quantified by gas chromatography coupled with mass spectrometry and gas chromatography coupled with the flame ionization detector. Twenty clinical isolates and one standard strain of each species were analyzed: E. coli, K. pneumoniae and P. aeruginosa (n=63). The antibacterial potential was evaluated by broth microdilution tests. Thymol is the prevalent component (87.37%). The EO at a concentration of 312 μg/mL was effective in inhibiting 52% of E. coli strains and about 38% of K. pneumoniae. On the other hand, for P. aeruginosa a concentration of 2,500 μg/mL was needed to obtain growth inhibition of approximately 38% of the strains. Among the resistance genes detected, the highest prevalence was blaCTX-M 1/2, however, even with the presence of these genes, an antimicrobial action of this EO was observed. Therefore, the results of this study suggest that the EO of L. origanoides presented an efficient antibacterial action with potential use in the fight against infections by MDR microorganisms. |
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Inhibitory and bactericidal activities of Lippia origanoides essential oil against Escherichia coli, Klebsiella pneumoniae and Pseudomonas aeruginosa multidrug resistantActividades inhibitorias y bactericidas del aceite esencial de Lippia origanoides frente a Escherichia coli, Klebsiella pneumoniae y Pseudomonas aeruginosa multirresistenteAtividades inibitória e bactericida do óleo essencial de Lippia origanoides frente a Escherichia coli, Klebsiella pneumoniae e Pseudomonas aeruginosa multidrogas resistentesPhytotherapicsNatural productsInfectionsVerbenaceaeβ-lactamases.FitoterápicosProdutos naturaisInfecçõesVerbenaceaeβ-lactamase.Hierbas medicinalesProductos naturalesInfeccionesVerbenáceasβ-lactamasas.The objective of this study was to evaluate the antimicrobial potential of essential oil (EO) from L. origanoides against Gram-negative bacteria with multidrug resistance (MDR) phenotype and to correlate the results obtained with the genetic profile of these microorganisms. The oil was extracted from the leaves of this plant by steam dragging and condensation with Clevenger equipment, the chemical components were identified and quantified by gas chromatography coupled with mass spectrometry and gas chromatography coupled with the flame ionization detector. Twenty clinical isolates and one standard strain of each species were analyzed: E. coli, K. pneumoniae and P. aeruginosa (n=63). The antibacterial potential was evaluated by broth microdilution tests. Thymol is the prevalent component (87.37%). The EO at a concentration of 312 μg/mL was effective in inhibiting 52% of E. coli strains and about 38% of K. pneumoniae. On the other hand, for P. aeruginosa a concentration of 2,500 μg/mL was needed to obtain growth inhibition of approximately 38% of the strains. Among the resistance genes detected, the highest prevalence was blaCTX-M 1/2, however, even with the presence of these genes, an antimicrobial action of this EO was observed. Therefore, the results of this study suggest that the EO of L. origanoides presented an efficient antibacterial action with potential use in the fight against infections by MDR microorganisms.El objetivo de este estudio fue evaluar el potencial antimicrobiano del aceite esencial (AE) de L. origanoides frente a bacterias Gram negativas con fenotipo multirresistente (MDR) y correlacionar los resultados obtenidos con el perfil genético de estos microorganismos. El aceite fue extraído de las hojas de esta planta por arrastre de vapor y condensación con equipo Clevenger, los componentes químicos fueron identificados y cuantificados por cromatografía de gases acoplada a espectrometría de masas y cromatografía de gases acoplada a detector de ionización de llama. Se analizaron veinte aislados clínicos y una cepa estándar de cada especie: E. coli, K. pneumoniae y P. aeruginosa (n=63). El potencial antibacteriano se evaluó mediante pruebas de microdilución en caldo. El timol es el componente predominante (87,37%). El AE a una concentración de 312 μg/mL fue eficaz para inhibir el 52% de las cepas de E. coli y aproximadamente el 38% de K. pneumoniae. En cambio, para P. aeruginosa fue necesaria una concentración de 2.500 μg/mL para obtener una inhibición del crecimiento de aproximadamente el 38% de las cepas. Entre los genes de resistencia detectados, el más prevalente fue blaCTX-M 1/2, sin embargo, aun con la presencia de estos genes, se observó una acción antimicrobiana de este AE. Por lo tanto, los resultados de este estudio sugieren que el AE de L. origanoides presentó una acción antibacteriana eficiente con potencial uso en el combate a infecciones por microorganismos MDR.O objetivo deste estudo foi avaliar o potencial antimicrobiano do óleo essencial (OE) de L. origanoides contra bactérias Gram-negativas com fenótipo de multirresistência (MDR) e correlacionar os resultados obtidos com o perfil genético desses microrganismos. O óleo foi extraído das folhas desta planta por arraste de vapor e condensação com equipamento Clevenger, os componentes químicos foram identificados e quantificados por cromatografia gasosa acoplada à espectrometria de massas e cromatografia gasosa acoplada ao detector de ionização de chama. Foram analisados vinte isolados clínicos e uma cepa padrão de cada espécie: E. coli, K. pneumoniae e P. aeruginosa (n=63). O potencial antibacteriano foi avaliado por testes de microdiluição em caldo. O timol é o componente prevalente (87,37%). O OE na concentração de 312 μg/mL foi eficaz na inibição de 52% das cepas de E. coli e cerca de 38% de K. pneumoniae. Por outro lado, para P. aeruginosa foi necessária uma concentração de 2.500 μg/mL para obter inibição do crescimento de aproximadamente 38% das cepas. Dentre os genes de resistência detectados, o de maior prevalência foi o blaCTX-M 1/2, porém, mesmo com a presença desses genes, observou-se ação antimicrobiana deste OE. Portanto, os resultados deste estudo sugerem que o OE de L. origanoides apresentou uma ação antibacteriana eficiente com potencial de uso no combate a infecções por microrganismos MDR.Research, Society and Development2022-07-03info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://rsdjournal.org/index.php/rsd/article/view/3147810.33448/rsd-v11i9.31478Research, Society and Development; Vol. 11 No. 9; e6811931478Research, Society and Development; Vol. 11 Núm. 9; e6811931478Research, Society and Development; v. 11 n. 9; e68119314782525-3409reponame:Research, Society and Developmentinstname:Universidade Federal de Itajubá (UNIFEI)instacron:UNIFEIenghttps://rsdjournal.org/index.php/rsd/article/view/31478/26952Copyright (c) 2022 Ludimila Gomes Pinheiro; Raquel Oliveira dos Santos Fontenelle ; Tigressa Helena Soares Rodrigues; Vicente de Paulo Teixeira Pinto; Francisco Cesar Barroso Barbosahttps://creativecommons.org/licenses/by/4.0info:eu-repo/semantics/openAccessPinheiro, Ludimila GomesFontenelle , Raquel Oliveira dos Santos Rodrigues, Tigressa Helena SoaresPinto, Vicente de Paulo TeixeiraBarbosa, Francisco Cesar Barroso 2022-07-21T12:36:16Zoai:ojs.pkp.sfu.ca:article/31478Revistahttps://rsdjournal.org/index.php/rsd/indexPUBhttps://rsdjournal.org/index.php/rsd/oairsd.articles@gmail.com2525-34092525-3409opendoar:2024-01-17T09:47:48.447141Research, Society and Development - Universidade Federal de Itajubá (UNIFEI)false |
dc.title.none.fl_str_mv |
Inhibitory and bactericidal activities of Lippia origanoides essential oil against Escherichia coli, Klebsiella pneumoniae and Pseudomonas aeruginosa multidrug resistant Actividades inhibitorias y bactericidas del aceite esencial de Lippia origanoides frente a Escherichia coli, Klebsiella pneumoniae y Pseudomonas aeruginosa multirresistente Atividades inibitória e bactericida do óleo essencial de Lippia origanoides frente a Escherichia coli, Klebsiella pneumoniae e Pseudomonas aeruginosa multidrogas resistentes |
title |
Inhibitory and bactericidal activities of Lippia origanoides essential oil against Escherichia coli, Klebsiella pneumoniae and Pseudomonas aeruginosa multidrug resistant |
spellingShingle |
Inhibitory and bactericidal activities of Lippia origanoides essential oil against Escherichia coli, Klebsiella pneumoniae and Pseudomonas aeruginosa multidrug resistant Pinheiro, Ludimila Gomes Phytotherapics Natural products Infections Verbenaceae β-lactamases. Fitoterápicos Produtos naturais Infecções Verbenaceae β-lactamase. Hierbas medicinales Productos naturales Infecciones Verbenáceas β-lactamasas. |
title_short |
Inhibitory and bactericidal activities of Lippia origanoides essential oil against Escherichia coli, Klebsiella pneumoniae and Pseudomonas aeruginosa multidrug resistant |
title_full |
Inhibitory and bactericidal activities of Lippia origanoides essential oil against Escherichia coli, Klebsiella pneumoniae and Pseudomonas aeruginosa multidrug resistant |
title_fullStr |
Inhibitory and bactericidal activities of Lippia origanoides essential oil against Escherichia coli, Klebsiella pneumoniae and Pseudomonas aeruginosa multidrug resistant |
title_full_unstemmed |
Inhibitory and bactericidal activities of Lippia origanoides essential oil against Escherichia coli, Klebsiella pneumoniae and Pseudomonas aeruginosa multidrug resistant |
title_sort |
Inhibitory and bactericidal activities of Lippia origanoides essential oil against Escherichia coli, Klebsiella pneumoniae and Pseudomonas aeruginosa multidrug resistant |
author |
Pinheiro, Ludimila Gomes |
author_facet |
Pinheiro, Ludimila Gomes Fontenelle , Raquel Oliveira dos Santos Rodrigues, Tigressa Helena Soares Pinto, Vicente de Paulo Teixeira Barbosa, Francisco Cesar Barroso |
author_role |
author |
author2 |
Fontenelle , Raquel Oliveira dos Santos Rodrigues, Tigressa Helena Soares Pinto, Vicente de Paulo Teixeira Barbosa, Francisco Cesar Barroso |
author2_role |
author author author author |
dc.contributor.author.fl_str_mv |
Pinheiro, Ludimila Gomes Fontenelle , Raquel Oliveira dos Santos Rodrigues, Tigressa Helena Soares Pinto, Vicente de Paulo Teixeira Barbosa, Francisco Cesar Barroso |
dc.subject.por.fl_str_mv |
Phytotherapics Natural products Infections Verbenaceae β-lactamases. Fitoterápicos Produtos naturais Infecções Verbenaceae β-lactamase. Hierbas medicinales Productos naturales Infecciones Verbenáceas β-lactamasas. |
topic |
Phytotherapics Natural products Infections Verbenaceae β-lactamases. Fitoterápicos Produtos naturais Infecções Verbenaceae β-lactamase. Hierbas medicinales Productos naturales Infecciones Verbenáceas β-lactamasas. |
description |
The objective of this study was to evaluate the antimicrobial potential of essential oil (EO) from L. origanoides against Gram-negative bacteria with multidrug resistance (MDR) phenotype and to correlate the results obtained with the genetic profile of these microorganisms. The oil was extracted from the leaves of this plant by steam dragging and condensation with Clevenger equipment, the chemical components were identified and quantified by gas chromatography coupled with mass spectrometry and gas chromatography coupled with the flame ionization detector. Twenty clinical isolates and one standard strain of each species were analyzed: E. coli, K. pneumoniae and P. aeruginosa (n=63). The antibacterial potential was evaluated by broth microdilution tests. Thymol is the prevalent component (87.37%). The EO at a concentration of 312 μg/mL was effective in inhibiting 52% of E. coli strains and about 38% of K. pneumoniae. On the other hand, for P. aeruginosa a concentration of 2,500 μg/mL was needed to obtain growth inhibition of approximately 38% of the strains. Among the resistance genes detected, the highest prevalence was blaCTX-M 1/2, however, even with the presence of these genes, an antimicrobial action of this EO was observed. Therefore, the results of this study suggest that the EO of L. origanoides presented an efficient antibacterial action with potential use in the fight against infections by MDR microorganisms. |
publishDate |
2022 |
dc.date.none.fl_str_mv |
2022-07-03 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
https://rsdjournal.org/index.php/rsd/article/view/31478 10.33448/rsd-v11i9.31478 |
url |
https://rsdjournal.org/index.php/rsd/article/view/31478 |
identifier_str_mv |
10.33448/rsd-v11i9.31478 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
https://rsdjournal.org/index.php/rsd/article/view/31478/26952 |
dc.rights.driver.fl_str_mv |
https://creativecommons.org/licenses/by/4.0 info:eu-repo/semantics/openAccess |
rights_invalid_str_mv |
https://creativecommons.org/licenses/by/4.0 |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Research, Society and Development |
publisher.none.fl_str_mv |
Research, Society and Development |
dc.source.none.fl_str_mv |
Research, Society and Development; Vol. 11 No. 9; e6811931478 Research, Society and Development; Vol. 11 Núm. 9; e6811931478 Research, Society and Development; v. 11 n. 9; e6811931478 2525-3409 reponame:Research, Society and Development instname:Universidade Federal de Itajubá (UNIFEI) instacron:UNIFEI |
instname_str |
Universidade Federal de Itajubá (UNIFEI) |
instacron_str |
UNIFEI |
institution |
UNIFEI |
reponame_str |
Research, Society and Development |
collection |
Research, Society and Development |
repository.name.fl_str_mv |
Research, Society and Development - Universidade Federal de Itajubá (UNIFEI) |
repository.mail.fl_str_mv |
rsd.articles@gmail.com |
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1797052812590317568 |