Anti-inflamatory response of poultry challenged with LPS of Escherichia coli
Autor(a) principal: | |
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Data de Publicação: | 2020 |
Outros Autores: | , , , , , |
Tipo de documento: | Artigo |
Idioma: | por |
Título da fonte: | Research, Society and Development |
Texto Completo: | https://rsdjournal.org/index.php/rsd/article/view/7823 |
Resumo: | To evaluate the anti-inflammatory response of poultry, body weight (BW), body weight loss (BWL), cloacal temperature (CT) and expression of interleukin-10 (IL-10) after LPS application were calculated. A total of 19-day-old male broilers were used. At 17 days, the broilers were weighed and distributed in 12 metal cages with 1 animal per experimental unit. Intraperitoneal application of LPS was realized through 1 ml/kg of body weight at 19 days of age at time zero. For each treatment, that is, each time after the application of the LPS, the temperature of the poultry was measured. Afterwards, this chicken was weighed to determine body weight and body weight loss. Finally, the broiler was euthanized by cervical dislocation and collected their spleens for analysis of IL-10 mRNA expression. The analysis of the results obtained during the performance of this work concludes that the application of LPS of Escherichia coli directly interferes in the body weight loss of the animals (Y = - 0.09617 - 1.54780 x + 0.19107 x2) in 3.2307 % after 4.05 hours of application, and which directly interferes with the Gene Expression of the Interleukin-10 (Y = 1.33367 + 3.23013 x - 0.31733 x2), having its peak of expession after 5,0895 hours of application with a concentration of 9,53364 A.U.. |
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Research, Society and Development |
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Anti-inflamatory response of poultry challenged with LPS of Escherichia coliRespuesta antiinflamatoria de pollos de corte difíciles com Escherichia coli LPSResposta anti-inflamatória de frangos de corte desafiados com LPS de Escherichia coliLPS de Escherichia coliInterleucina-10IL-10.Escherichia coli LPSInterleucina-10IL-10.Escherichia coli LPSInterleukin-10IL-10.To evaluate the anti-inflammatory response of poultry, body weight (BW), body weight loss (BWL), cloacal temperature (CT) and expression of interleukin-10 (IL-10) after LPS application were calculated. A total of 19-day-old male broilers were used. At 17 days, the broilers were weighed and distributed in 12 metal cages with 1 animal per experimental unit. Intraperitoneal application of LPS was realized through 1 ml/kg of body weight at 19 days of age at time zero. For each treatment, that is, each time after the application of the LPS, the temperature of the poultry was measured. Afterwards, this chicken was weighed to determine body weight and body weight loss. Finally, the broiler was euthanized by cervical dislocation and collected their spleens for analysis of IL-10 mRNA expression. The analysis of the results obtained during the performance of this work concludes that the application of LPS of Escherichia coli directly interferes in the body weight loss of the animals (Y = - 0.09617 - 1.54780 x + 0.19107 x2) in 3.2307 % after 4.05 hours of application, and which directly interferes with the Gene Expression of the Interleukin-10 (Y = 1.33367 + 3.23013 x - 0.31733 x2), having its peak of expession after 5,0895 hours of application with a concentration of 9,53364 A.U..Para evaluar la respuesta antiinflamatoria de los pollos de engorde, se calcularon el peso final (PF), la pérdida de peso (PP), la temperatura rectal (TR) y la expresión de interleucina-10 (IL-10) después de la aplicación de LPS. Se utilizaron doce pollos de engorde de 19 días. A los 17 días, las aves fueron pesadas y distribuidas en 12 jaulas metálicas con 1 animal por unidad experimental. La aplicación intraperitoneal de LPS se realizó a una dosis de 1 ml / kg de peso vivo de aves a los 19 días de edad en el momento cero. Para cada tratamiento, es decir, cada vez después de aplicar el LPS, se midió la temperatura de las aves. Posteriormente, se pesó esta ave para determinar el peso final y la pérdida de peso. Finalmente, se sacrificó el ave y se recogió su bazo para el análisis de la expresión de ARNm de IL-10. El análisis de los resultados obtenidos durante la realización de este trabajo concluye que la aplicación de LPS de Escherichia coli interfiere directamente en la pérdida de peso de los animales (Y = - 0.09617 - 1.54780 x + 0.19107 x2) hasta 3.2307 % después de 4.05 horas de aplicación, y que interfiere directamente con la expresión génica de la citocina antiinflamatoria Interleucina-10 (Y = 1.33367 + 3.23013 x - 0.31733 x2), que tiene su pico de expresión después de 5, 0895 horas de aplicación con una concentración de 9,53361 A.U..Para avaliar a resposta anti-inflamatória de frangos de corte foram calculados peso final (PF), perda de peso (PP), temperatura retal (TR) e expressão de interleucina-10 (IL-10) após a aplicação de LPS. Foram utilizados 12 frangos de corte machos com 19 dias de idade. Aos 17 dias, as aves foram pesadas e distribuídas em 12 gaiolas de metal com 1 animal por unidade experimental. Foi realizada a aplicação intraperitoneal do LPS na dose de 1 ml/kg de peso vivo das aves aos 19 dias de idade no tempo zero. Para cada tratamento, ou seja, cada tempo após a aplicação do LPS, foi mensurada a temperatura das aves. Após, essa ave foi pesada para determinação de Peso Final e Perda de Peso. Finalmente, a ave foi abatida e realizada a coleta de seu baço para análise de expressão de RNAm de IL-10. A análise dos resultados obtidos durante a realização deste trabalho conclui que a aplicação de LPS de Escherichia coli interfere diretamente na perda de peso dos animais (Y = - 0,09617 - 1,54780 x + 0,19107 x2) até em 3,2307% após 4,05 horas da aplicação, e que interfere diretamente a Expressão Gênica da citocina anti-inflamatória Interleucina-10 (Y = 1,33367 + 3,23013 x - 0,31733 x2), tendo seu pico de expressão após 5,0895 horas da aplicação com a concentração de 9,53361 U.A..Research, Society and Development2020-09-05info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://rsdjournal.org/index.php/rsd/article/view/782310.33448/rsd-v9i9.7823Research, Society and Development; Vol. 9 No. 9; e732997823Research, Society and Development; Vol. 9 Núm. 9; e732997823Research, Society and Development; v. 9 n. 9; e7329978232525-3409reponame:Research, Society and Developmentinstname:Universidade Federal de Itajubá (UNIFEI)instacron:UNIFEIporhttps://rsdjournal.org/index.php/rsd/article/view/7823/6943Copyright (c) 2020 Samuel Oliveira Borges; Rayanne Andrade Nunes; Beatriz Garcia do Vale; Bruno Figueiredo de Almeida; Thiago Ferreira Diana; Romário Duarte Bernardes; Arele Arlindo Calderanohttps://creativecommons.org/licenses/by/4.0info:eu-repo/semantics/openAccessBorges, Samuel OliveiraNunes, Rayanne AndradeVale, Beatriz Garcia do Almeida, Bruno Figueiredo de Diana, Thiago Ferreira Bernardes, Romário Duarte Calderano, Arele Arlindo 2020-09-18T01:42:11Zoai:ojs.pkp.sfu.ca:article/7823Revistahttps://rsdjournal.org/index.php/rsd/indexPUBhttps://rsdjournal.org/index.php/rsd/oairsd.articles@gmail.com2525-34092525-3409opendoar:2024-01-17T09:30:25.839914Research, Society and Development - Universidade Federal de Itajubá (UNIFEI)false |
dc.title.none.fl_str_mv |
Anti-inflamatory response of poultry challenged with LPS of Escherichia coli Respuesta antiinflamatoria de pollos de corte difíciles com Escherichia coli LPS Resposta anti-inflamatória de frangos de corte desafiados com LPS de Escherichia coli |
title |
Anti-inflamatory response of poultry challenged with LPS of Escherichia coli |
spellingShingle |
Anti-inflamatory response of poultry challenged with LPS of Escherichia coli Borges, Samuel Oliveira LPS de Escherichia coli Interleucina-10 IL-10. Escherichia coli LPS Interleucina-10 IL-10. Escherichia coli LPS Interleukin-10 IL-10. |
title_short |
Anti-inflamatory response of poultry challenged with LPS of Escherichia coli |
title_full |
Anti-inflamatory response of poultry challenged with LPS of Escherichia coli |
title_fullStr |
Anti-inflamatory response of poultry challenged with LPS of Escherichia coli |
title_full_unstemmed |
Anti-inflamatory response of poultry challenged with LPS of Escherichia coli |
title_sort |
Anti-inflamatory response of poultry challenged with LPS of Escherichia coli |
author |
Borges, Samuel Oliveira |
author_facet |
Borges, Samuel Oliveira Nunes, Rayanne Andrade Vale, Beatriz Garcia do Almeida, Bruno Figueiredo de Diana, Thiago Ferreira Bernardes, Romário Duarte Calderano, Arele Arlindo |
author_role |
author |
author2 |
Nunes, Rayanne Andrade Vale, Beatriz Garcia do Almeida, Bruno Figueiredo de Diana, Thiago Ferreira Bernardes, Romário Duarte Calderano, Arele Arlindo |
author2_role |
author author author author author author |
dc.contributor.author.fl_str_mv |
Borges, Samuel Oliveira Nunes, Rayanne Andrade Vale, Beatriz Garcia do Almeida, Bruno Figueiredo de Diana, Thiago Ferreira Bernardes, Romário Duarte Calderano, Arele Arlindo |
dc.subject.por.fl_str_mv |
LPS de Escherichia coli Interleucina-10 IL-10. Escherichia coli LPS Interleucina-10 IL-10. Escherichia coli LPS Interleukin-10 IL-10. |
topic |
LPS de Escherichia coli Interleucina-10 IL-10. Escherichia coli LPS Interleucina-10 IL-10. Escherichia coli LPS Interleukin-10 IL-10. |
description |
To evaluate the anti-inflammatory response of poultry, body weight (BW), body weight loss (BWL), cloacal temperature (CT) and expression of interleukin-10 (IL-10) after LPS application were calculated. A total of 19-day-old male broilers were used. At 17 days, the broilers were weighed and distributed in 12 metal cages with 1 animal per experimental unit. Intraperitoneal application of LPS was realized through 1 ml/kg of body weight at 19 days of age at time zero. For each treatment, that is, each time after the application of the LPS, the temperature of the poultry was measured. Afterwards, this chicken was weighed to determine body weight and body weight loss. Finally, the broiler was euthanized by cervical dislocation and collected their spleens for analysis of IL-10 mRNA expression. The analysis of the results obtained during the performance of this work concludes that the application of LPS of Escherichia coli directly interferes in the body weight loss of the animals (Y = - 0.09617 - 1.54780 x + 0.19107 x2) in 3.2307 % after 4.05 hours of application, and which directly interferes with the Gene Expression of the Interleukin-10 (Y = 1.33367 + 3.23013 x - 0.31733 x2), having its peak of expession after 5,0895 hours of application with a concentration of 9,53364 A.U.. |
publishDate |
2020 |
dc.date.none.fl_str_mv |
2020-09-05 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
https://rsdjournal.org/index.php/rsd/article/view/7823 10.33448/rsd-v9i9.7823 |
url |
https://rsdjournal.org/index.php/rsd/article/view/7823 |
identifier_str_mv |
10.33448/rsd-v9i9.7823 |
dc.language.iso.fl_str_mv |
por |
language |
por |
dc.relation.none.fl_str_mv |
https://rsdjournal.org/index.php/rsd/article/view/7823/6943 |
dc.rights.driver.fl_str_mv |
https://creativecommons.org/licenses/by/4.0 info:eu-repo/semantics/openAccess |
rights_invalid_str_mv |
https://creativecommons.org/licenses/by/4.0 |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Research, Society and Development |
publisher.none.fl_str_mv |
Research, Society and Development |
dc.source.none.fl_str_mv |
Research, Society and Development; Vol. 9 No. 9; e732997823 Research, Society and Development; Vol. 9 Núm. 9; e732997823 Research, Society and Development; v. 9 n. 9; e732997823 2525-3409 reponame:Research, Society and Development instname:Universidade Federal de Itajubá (UNIFEI) instacron:UNIFEI |
instname_str |
Universidade Federal de Itajubá (UNIFEI) |
instacron_str |
UNIFEI |
institution |
UNIFEI |
reponame_str |
Research, Society and Development |
collection |
Research, Society and Development |
repository.name.fl_str_mv |
Research, Society and Development - Universidade Federal de Itajubá (UNIFEI) |
repository.mail.fl_str_mv |
rsd.articles@gmail.com |
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1797052780474531840 |