Enzyme activity of the tilapia (Oreochromis niloticus) antioxidant defense system as a model of exposure to the titanium dioxide (TiO2) nanoparticle
Autor(a) principal: | |
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Data de Publicação: | 2021 |
Outros Autores: | , |
Tipo de documento: | Artigo |
Idioma: | por |
Título da fonte: | Research, Society and Development |
Texto Completo: | https://rsdjournal.org/index.php/rsd/article/view/12829 |
Resumo: | The biochemical biomarkers of fish can be useful tools in the investigation of the presence of nanoparticles (NPs) in the aquatic system. However, it is extremely important to know the responses of these biomarkers to exposure to this xenobiotic in order to try to establish a model for biological monitoring. Thus, our objective was to determine the response of the enzymes catalase (CAT) and glutathione S-transferase (GST) of tilapia (Oreochromis niloticus) after 48 and 96 hours of exposure to a titanium dioxide nanoparticle (NP-TiO2). For this purpose, 10 fish were exposed to: 0 (control), 1, 5, 10 and 50 mg.L-1 of NP-TiO2. Then, the liver was used to determine the biomarkers. We did not observe a significant variation in CAT in tilapia exposed for 48h to PN. However, after 96 h the CAT was higher in the exposed animals at the highest tested concentrations (10 and 50 mg.L-1). Even at these concentrations, the activity was higher in the animals exposed for 96 hours. For GST, in the first 48 h the activity was lower in animals exposed to 1, 5 and 50 mg.L-1 of TiO2, whereas, in the exposure of 96 h, greater activity of GST was observed in animals exposed to 50 mg.L-1. Comparing the exposure time, in all concentrations tested, the hepatic GST activity was higher in the animals exposed for 96 h. Thus, in addition to the concentration, the time of exposure to NP seems to influence the response of the enzymes, increasing their activity. |
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Enzyme activity of the tilapia (Oreochromis niloticus) antioxidant defense system as a model of exposure to the titanium dioxide (TiO2) nanoparticleActividad enzimática del sistema de defensa antioxidante de la tilapia (Oreochromis niloticus) como modelo de exposición a la nanopartícula de dióxido de titanio (TiO2)Atividade de enzimas do sistema de defesa antioxidante de tilápias (Oreochromis niloticus) como modelo de exposição à nanopartícula de dióxido de titânio (TiO2)NanoecotoxicologiaEnzimas antioxidantesCatalaseGlutationa S-transferase.NanoecotoxicologyAntioxidant enzymesCatalaseGlutathione S-transferase.NanoecotoxicologíaEnzimas antioxidantesCatalaseGlutatión S-transferase.The biochemical biomarkers of fish can be useful tools in the investigation of the presence of nanoparticles (NPs) in the aquatic system. However, it is extremely important to know the responses of these biomarkers to exposure to this xenobiotic in order to try to establish a model for biological monitoring. Thus, our objective was to determine the response of the enzymes catalase (CAT) and glutathione S-transferase (GST) of tilapia (Oreochromis niloticus) after 48 and 96 hours of exposure to a titanium dioxide nanoparticle (NP-TiO2). For this purpose, 10 fish were exposed to: 0 (control), 1, 5, 10 and 50 mg.L-1 of NP-TiO2. Then, the liver was used to determine the biomarkers. We did not observe a significant variation in CAT in tilapia exposed for 48h to PN. However, after 96 h the CAT was higher in the exposed animals at the highest tested concentrations (10 and 50 mg.L-1). Even at these concentrations, the activity was higher in the animals exposed for 96 hours. For GST, in the first 48 h the activity was lower in animals exposed to 1, 5 and 50 mg.L-1 of TiO2, whereas, in the exposure of 96 h, greater activity of GST was observed in animals exposed to 50 mg.L-1. Comparing the exposure time, in all concentrations tested, the hepatic GST activity was higher in the animals exposed for 96 h. Thus, in addition to the concentration, the time of exposure to NP seems to influence the response of the enzymes, increasing their activity.Los biomarcadores bioquímicos de los peces pueden ser herramientas útiles en la investigación de la presencia de nanopartículas (NP) en el sistema acuático. Sin embargo, es de suma importancia conocer las respuestas de estos biomarcadores a la exposición a este xenobiótico para intentar establecer un modelo de seguimiento biológico. Así, nuestro objetivo fue determinar la respuesta de las enzimas catalase (CAT) y glutatión S-transferase (GST) de tilapia (Oreochromis niloticus) tras 48 y 96 horas de exposición a una nanopartícula de dióxido de titanio (NP-TiO2). Para ello, se expusieron 10 peces a: 0 (control), 1, 5, 10 y 50 mg.L-1 de NP-TiO2. Luego, se utilizó el hígado para determinar los biomarcadores. No observamos una variación significativa en CAT en tilapia expuesta durante 48 h a NP. Sin embargo, después de 96 h, el CAT fue mayor en los animales expuestos a las concentraciones más altas probadas (10 y 50 mg.L-1). Incluso a estas concentraciones, la actividad fue mayor en los animales expuestos durante 96 h. Para GST, en las primeras 48 h la actividad fue menor en animales expuestos a 1, 5 y 50 mg.L-1 de TiO2, mientras que, en la exposición de 96 h, se observó mayor actividad de GST en animales expuestos a 50 mg.L-1. Comparando el tiempo de exposición, en todas las concentraciones probadas, la actividad de GST hepática fue mayor en los animales expuestos durante 96 h. Así, además de la concentración, el tiempo de exposición a NP parece influir en la respuesta de las enzimas, aumentando su actividad.Os biomarcadores bioquímicos de peixes podem ser ferramentas úteis na investigação da presença de nanopartículas (NPs) no sistema aquático. Entretanto, é de suma importância conhecer as respostas desses biomarcadores frente a exposição a esse xenobiotico para se tentar estabelecer um modelo para monitoramento biológico. Assim, nosso objetivo foi determinar a resposta das enzimas catalase (CAT) e glutationa S-transferase (GST) de tilápias (Oreochromis niloticus) após 48 e 96h de exposição a nanopartícula de dióxido de titânio (NP-TiO2). Para tanto, 10 peixes foram expostos a: 0 (controle), 1, 5, 10 e 50 mg.L-1 da NP-TiO2. Em seguida, o fígado foi utilizado para a determinação dos biomarcadores. Nós não observamos variação significativa da CAT nas tilápias expostas durante 48h a NP. Entretanto, após 96h a CAT foi maior nos animais expostos as maiores concentrações testadas (10 e 50 mg.L-1). Ainda nessas concentrações, a atividade foi maior nos animais expostos durante 96h. Para a GST, nas primeiras 48h a atividade foi menor nos animais expostos a 1, 5 e 50 mg.L-1 de TiO2, enquanto que, na exposição de 96 h a maior atividade da GST foi observada nos animais expostos a 50 mg.L-1. Comparando-se o tempo de exposição, em todas as concentrações testadas, a atividade da GST hepática foi maior nos animais expostos durante 96h. Assim, além da concentração, o tempo de exposição a NP parece influenciar na resposta das enzimas, aumentando sua atividade.Research, Society and Development2021-05-13info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://rsdjournal.org/index.php/rsd/article/view/1282910.33448/rsd-v10i5.12829Research, Society and Development; Vol. 10 No. 5; e46810512829Research, Society and Development; Vol. 10 Núm. 5; e46810512829Research, Society and Development; v. 10 n. 5; e468105128292525-3409reponame:Research, Society and Developmentinstname:Universidade Federal de Itajubá (UNIFEI)instacron:UNIFEIporhttps://rsdjournal.org/index.php/rsd/article/view/12829/13602Copyright (c) 2021 Theila dos Santos Santana; Marilene Bárbara dos Santos; Elissandra Ulbricht Winkalerhttps://creativecommons.org/licenses/by/4.0info:eu-repo/semantics/openAccessSantana, Theila dos Santos Santos, Marilene Bárbara dos Winkaler, Elissandra Ulbricht 2021-05-17T18:20:49Zoai:ojs.pkp.sfu.ca:article/12829Revistahttps://rsdjournal.org/index.php/rsd/indexPUBhttps://rsdjournal.org/index.php/rsd/oairsd.articles@gmail.com2525-34092525-3409opendoar:2024-01-17T09:34:18.490974Research, Society and Development - Universidade Federal de Itajubá (UNIFEI)false |
dc.title.none.fl_str_mv |
Enzyme activity of the tilapia (Oreochromis niloticus) antioxidant defense system as a model of exposure to the titanium dioxide (TiO2) nanoparticle Actividad enzimática del sistema de defensa antioxidante de la tilapia (Oreochromis niloticus) como modelo de exposición a la nanopartícula de dióxido de titanio (TiO2) Atividade de enzimas do sistema de defesa antioxidante de tilápias (Oreochromis niloticus) como modelo de exposição à nanopartícula de dióxido de titânio (TiO2) |
title |
Enzyme activity of the tilapia (Oreochromis niloticus) antioxidant defense system as a model of exposure to the titanium dioxide (TiO2) nanoparticle |
spellingShingle |
Enzyme activity of the tilapia (Oreochromis niloticus) antioxidant defense system as a model of exposure to the titanium dioxide (TiO2) nanoparticle Santana, Theila dos Santos Nanoecotoxicologia Enzimas antioxidantes Catalase Glutationa S-transferase. Nanoecotoxicology Antioxidant enzymes Catalase Glutathione S-transferase. Nanoecotoxicología Enzimas antioxidantes Catalase Glutatión S-transferase. |
title_short |
Enzyme activity of the tilapia (Oreochromis niloticus) antioxidant defense system as a model of exposure to the titanium dioxide (TiO2) nanoparticle |
title_full |
Enzyme activity of the tilapia (Oreochromis niloticus) antioxidant defense system as a model of exposure to the titanium dioxide (TiO2) nanoparticle |
title_fullStr |
Enzyme activity of the tilapia (Oreochromis niloticus) antioxidant defense system as a model of exposure to the titanium dioxide (TiO2) nanoparticle |
title_full_unstemmed |
Enzyme activity of the tilapia (Oreochromis niloticus) antioxidant defense system as a model of exposure to the titanium dioxide (TiO2) nanoparticle |
title_sort |
Enzyme activity of the tilapia (Oreochromis niloticus) antioxidant defense system as a model of exposure to the titanium dioxide (TiO2) nanoparticle |
author |
Santana, Theila dos Santos |
author_facet |
Santana, Theila dos Santos Santos, Marilene Bárbara dos Winkaler, Elissandra Ulbricht |
author_role |
author |
author2 |
Santos, Marilene Bárbara dos Winkaler, Elissandra Ulbricht |
author2_role |
author author |
dc.contributor.author.fl_str_mv |
Santana, Theila dos Santos Santos, Marilene Bárbara dos Winkaler, Elissandra Ulbricht |
dc.subject.por.fl_str_mv |
Nanoecotoxicologia Enzimas antioxidantes Catalase Glutationa S-transferase. Nanoecotoxicology Antioxidant enzymes Catalase Glutathione S-transferase. Nanoecotoxicología Enzimas antioxidantes Catalase Glutatión S-transferase. |
topic |
Nanoecotoxicologia Enzimas antioxidantes Catalase Glutationa S-transferase. Nanoecotoxicology Antioxidant enzymes Catalase Glutathione S-transferase. Nanoecotoxicología Enzimas antioxidantes Catalase Glutatión S-transferase. |
description |
The biochemical biomarkers of fish can be useful tools in the investigation of the presence of nanoparticles (NPs) in the aquatic system. However, it is extremely important to know the responses of these biomarkers to exposure to this xenobiotic in order to try to establish a model for biological monitoring. Thus, our objective was to determine the response of the enzymes catalase (CAT) and glutathione S-transferase (GST) of tilapia (Oreochromis niloticus) after 48 and 96 hours of exposure to a titanium dioxide nanoparticle (NP-TiO2). For this purpose, 10 fish were exposed to: 0 (control), 1, 5, 10 and 50 mg.L-1 of NP-TiO2. Then, the liver was used to determine the biomarkers. We did not observe a significant variation in CAT in tilapia exposed for 48h to PN. However, after 96 h the CAT was higher in the exposed animals at the highest tested concentrations (10 and 50 mg.L-1). Even at these concentrations, the activity was higher in the animals exposed for 96 hours. For GST, in the first 48 h the activity was lower in animals exposed to 1, 5 and 50 mg.L-1 of TiO2, whereas, in the exposure of 96 h, greater activity of GST was observed in animals exposed to 50 mg.L-1. Comparing the exposure time, in all concentrations tested, the hepatic GST activity was higher in the animals exposed for 96 h. Thus, in addition to the concentration, the time of exposure to NP seems to influence the response of the enzymes, increasing their activity. |
publishDate |
2021 |
dc.date.none.fl_str_mv |
2021-05-13 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
https://rsdjournal.org/index.php/rsd/article/view/12829 10.33448/rsd-v10i5.12829 |
url |
https://rsdjournal.org/index.php/rsd/article/view/12829 |
identifier_str_mv |
10.33448/rsd-v10i5.12829 |
dc.language.iso.fl_str_mv |
por |
language |
por |
dc.relation.none.fl_str_mv |
https://rsdjournal.org/index.php/rsd/article/view/12829/13602 |
dc.rights.driver.fl_str_mv |
https://creativecommons.org/licenses/by/4.0 info:eu-repo/semantics/openAccess |
rights_invalid_str_mv |
https://creativecommons.org/licenses/by/4.0 |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Research, Society and Development |
publisher.none.fl_str_mv |
Research, Society and Development |
dc.source.none.fl_str_mv |
Research, Society and Development; Vol. 10 No. 5; e46810512829 Research, Society and Development; Vol. 10 Núm. 5; e46810512829 Research, Society and Development; v. 10 n. 5; e46810512829 2525-3409 reponame:Research, Society and Development instname:Universidade Federal de Itajubá (UNIFEI) instacron:UNIFEI |
instname_str |
Universidade Federal de Itajubá (UNIFEI) |
instacron_str |
UNIFEI |
institution |
UNIFEI |
reponame_str |
Research, Society and Development |
collection |
Research, Society and Development |
repository.name.fl_str_mv |
Research, Society and Development - Universidade Federal de Itajubá (UNIFEI) |
repository.mail.fl_str_mv |
rsd.articles@gmail.com |
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1797052783944269824 |