Definition of protocol and statistical techniques to optimize the in vitro establishment of Passiflora foetida
Autor(a) principal: | |
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Data de Publicação: | 2020 |
Outros Autores: | , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | por |
Título da fonte: | Research, Society and Development |
Texto Completo: | https://rsdjournal.org/index.php/rsd/article/view/9378 |
Resumo: | Passiflora foetida, a species of wild passion fruit, has been investigated due to its pharmaceutical potential and its agronomic characteristic of resistance to Fusarium spp., enabling its use as a rootstock for passion fruit species susceptible to premature death. Thus, in order to perform the propagation of this species, micropropagation is one of the most used forms, being necessary the use of accessions of a matrix plant to, by organogenesis, generate new seedlings with the same genotype. Thus, the aim of the present study was to establish a protocol for the in vitro propagation of P. foetida, inducing the buds of this plant, by organogenesis, testing two concentrations of mineral salts from MS medium, without addition of phytohormones. For in vitro establishment, apical and lateral buds obtained from plants of P. foetida, grown in a greenhouse, were used. The experiments were carried out in a completely randomized design. Sixty days after inoculation of explants, the following criteria were evaluated: number of buds, length of sprouting, number of leaves, coloration score, developmental score and root number. To guarantee the accuracy of the results, statistical techniques were applied to data analysis and optimization of results. The development of plants by organogenesis showed statistically similar values in both concentrations, concluding that the MS medium with half concentrations of nutrients was considered the most viable for the in vitro establishment of this species. |
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Definition of protocol and statistical techniques to optimize the in vitro establishment of Passiflora foetidaDefinición de protocolo y técnicas estadísticas para optimizar el establecimiento in vitro de Passiflora foetidaDefinição do protocolo e técnicas estatísticas para otimizar o estabelecimento in vitro de Passiflora foetidaPassifloraceaMicropropagaçãoOrganogênese.PassifloraceaMicropropagaciónOrganogénesis.PassifloraceaMicropropagationOrganogenesis.Passiflora foetida, a species of wild passion fruit, has been investigated due to its pharmaceutical potential and its agronomic characteristic of resistance to Fusarium spp., enabling its use as a rootstock for passion fruit species susceptible to premature death. Thus, in order to perform the propagation of this species, micropropagation is one of the most used forms, being necessary the use of accessions of a matrix plant to, by organogenesis, generate new seedlings with the same genotype. Thus, the aim of the present study was to establish a protocol for the in vitro propagation of P. foetida, inducing the buds of this plant, by organogenesis, testing two concentrations of mineral salts from MS medium, without addition of phytohormones. For in vitro establishment, apical and lateral buds obtained from plants of P. foetida, grown in a greenhouse, were used. The experiments were carried out in a completely randomized design. Sixty days after inoculation of explants, the following criteria were evaluated: number of buds, length of sprouting, number of leaves, coloration score, developmental score and root number. To guarantee the accuracy of the results, statistical techniques were applied to data analysis and optimization of results. The development of plants by organogenesis showed statistically similar values in both concentrations, concluding that the MS medium with half concentrations of nutrients was considered the most viable for the in vitro establishment of this species.Passiflora foetida, una especie de maracuyá silvestre, ha sido investigada por su potencial farmacéutico y su característica agronómica de resistencia a Fusarium spp., Permitiendo su uso como patrón para especies de maracuyá susceptibles de muerte prematura. Así, apuntando a la propagación de esta especie, la micropropagación es una de las formas más utilizadas, siendo necesario el uso de acceso de una planta matriz por organogénesis y generar nuevas plántulas con el mismo genotipo. Así, este estudio tuvo como objetivo establecer un protocolo para la propagación in vitro de P. foetida por inducción de yema por organogénesis, probando dos concentraciones de sales minerales del medio MS, sin añadir fitohormonas. Para el establecimiento in vitro se utilizaron yemas apicales y laterales obtenidas de plantas de P. foetida, cultivadas en invernadero. Los experimentos se llevaron a cabo en un diseño completamente al azar. Sesenta días después de la inoculación de los explantes, se evaluó lo siguiente: número de yemas, longitud de brotación, número de hojas, puntuación de coloración, puntuación de desarrollo y número de raíces. Para garantizar la exactitud de los resultados, se aplicarón técnicas estadísticas de análisis de datos y optimización de resultados. El desarrollo de plantas por organogénesis mostró valores estadísticamente similares en ambas concentraciones, concluyendo que el medio MS con la mitad de concentraciones de nutrientes fue considerado el más viable para el establecimiento in vitro de esta especie.Passiflora foetida, uma espécie de maracujá silvestre, tem sido pesquisado devido ao seu potencial farmacêutico e sua característica agronômica de resistência ao Fusarium spp., possibilitando a sua utilização como porta-enxerto para espécies de maracujá suscetíveis a morte prematura. Assim, visando a propagação dessa espécie, a micropropagação é uma das formas mais utilizadas, sendo necessário a utilização de acessos de uma planta matriz para, por organogênese, gerar novas plântulas com o mesmo genótipo. Desta forma, este trabalho teve como objetivo estabelecer protocolo para a propagação in vitro de P. foetida pela indução de gemas por organogênese, testando-se duas concentrações de sais minerais do meio MS, sem adição de fitormônios. Para o estabelecimento in vitro foram utilizados gemas apicais e laterais obtidas a partir de plantas matrizes de P. foetida, cultivadas em casa de vegetação. Os experimentos foram realizados em delineamento inteiramente casualizado. Aos 60 dias após a inoculação dos explantes foram avaliados: número de gemas, comprimento da brotação, número de folhas, escore de coloração, escore de desenvolvimento e número de raiz. Para garantir a precisão dos resultados foram realizadas técnicas estatísticas aplicadas à análise de dados e otimização dos resultados. O desenvolvimento das plantas por organogênese apresentou valores estatisticamente similares em ambas as concentrações, podendo concluir que o meio MS com metade das concentrações de nutrientes foi o mais viável para o estabelecimento in vitro dessa espécie.Research, Society and Development2020-10-29info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://rsdjournal.org/index.php/rsd/article/view/937810.33448/rsd-v9i10.9378Research, Society and Development; Vol. 9 No. 10; e9689109378Research, Society and Development; Vol. 9 Núm. 10; e9689109378Research, Society and Development; v. 9 n. 10; e96891093782525-3409reponame:Research, Society and Developmentinstname:Universidade Federal de Itajubá (UNIFEI)instacron:UNIFEIporhttps://rsdjournal.org/index.php/rsd/article/view/9378/8421Copyright (c) 2020 Glaucia Amorim Faria; Luiz Henrique Silvério Junior; Beatriz Garcia Lopes; Antonio Flavio Arruda Ferreira; Cíntia Patrícia Martins de Oliveira; Paula Suares Rocha; Taís Ferreira Costa ; Enes Furlani Junior; Lucas Menezes Felizardohttps://creativecommons.org/licenses/by/4.0info:eu-repo/semantics/openAccessFaria, Glaucia Amorim Silvério Junior, Luiz Henrique Lopes, Beatriz Garcia Ferreira, Antonio Flavio Arruda Oliveira, Cíntia Patrícia Martins de Rocha, Paula Suares Costa , Taís Ferreira Furlani Junior, EnesFelizardo, Lucas Menezes 2020-10-31T12:03:23Zoai:ojs.pkp.sfu.ca:article/9378Revistahttps://rsdjournal.org/index.php/rsd/indexPUBhttps://rsdjournal.org/index.php/rsd/oairsd.articles@gmail.com2525-34092525-3409opendoar:2024-01-17T09:31:40.768826Research, Society and Development - Universidade Federal de Itajubá (UNIFEI)false |
dc.title.none.fl_str_mv |
Definition of protocol and statistical techniques to optimize the in vitro establishment of Passiflora foetida Definición de protocolo y técnicas estadísticas para optimizar el establecimiento in vitro de Passiflora foetida Definição do protocolo e técnicas estatísticas para otimizar o estabelecimento in vitro de Passiflora foetida |
title |
Definition of protocol and statistical techniques to optimize the in vitro establishment of Passiflora foetida |
spellingShingle |
Definition of protocol and statistical techniques to optimize the in vitro establishment of Passiflora foetida Faria, Glaucia Amorim Passifloracea Micropropagação Organogênese. Passifloracea Micropropagación Organogénesis. Passifloracea Micropropagation Organogenesis. |
title_short |
Definition of protocol and statistical techniques to optimize the in vitro establishment of Passiflora foetida |
title_full |
Definition of protocol and statistical techniques to optimize the in vitro establishment of Passiflora foetida |
title_fullStr |
Definition of protocol and statistical techniques to optimize the in vitro establishment of Passiflora foetida |
title_full_unstemmed |
Definition of protocol and statistical techniques to optimize the in vitro establishment of Passiflora foetida |
title_sort |
Definition of protocol and statistical techniques to optimize the in vitro establishment of Passiflora foetida |
author |
Faria, Glaucia Amorim |
author_facet |
Faria, Glaucia Amorim Silvério Junior, Luiz Henrique Lopes, Beatriz Garcia Ferreira, Antonio Flavio Arruda Oliveira, Cíntia Patrícia Martins de Rocha, Paula Suares Costa , Taís Ferreira Furlani Junior, Enes Felizardo, Lucas Menezes |
author_role |
author |
author2 |
Silvério Junior, Luiz Henrique Lopes, Beatriz Garcia Ferreira, Antonio Flavio Arruda Oliveira, Cíntia Patrícia Martins de Rocha, Paula Suares Costa , Taís Ferreira Furlani Junior, Enes Felizardo, Lucas Menezes |
author2_role |
author author author author author author author author |
dc.contributor.author.fl_str_mv |
Faria, Glaucia Amorim Silvério Junior, Luiz Henrique Lopes, Beatriz Garcia Ferreira, Antonio Flavio Arruda Oliveira, Cíntia Patrícia Martins de Rocha, Paula Suares Costa , Taís Ferreira Furlani Junior, Enes Felizardo, Lucas Menezes |
dc.subject.por.fl_str_mv |
Passifloracea Micropropagação Organogênese. Passifloracea Micropropagación Organogénesis. Passifloracea Micropropagation Organogenesis. |
topic |
Passifloracea Micropropagação Organogênese. Passifloracea Micropropagación Organogénesis. Passifloracea Micropropagation Organogenesis. |
description |
Passiflora foetida, a species of wild passion fruit, has been investigated due to its pharmaceutical potential and its agronomic characteristic of resistance to Fusarium spp., enabling its use as a rootstock for passion fruit species susceptible to premature death. Thus, in order to perform the propagation of this species, micropropagation is one of the most used forms, being necessary the use of accessions of a matrix plant to, by organogenesis, generate new seedlings with the same genotype. Thus, the aim of the present study was to establish a protocol for the in vitro propagation of P. foetida, inducing the buds of this plant, by organogenesis, testing two concentrations of mineral salts from MS medium, without addition of phytohormones. For in vitro establishment, apical and lateral buds obtained from plants of P. foetida, grown in a greenhouse, were used. The experiments were carried out in a completely randomized design. Sixty days after inoculation of explants, the following criteria were evaluated: number of buds, length of sprouting, number of leaves, coloration score, developmental score and root number. To guarantee the accuracy of the results, statistical techniques were applied to data analysis and optimization of results. The development of plants by organogenesis showed statistically similar values in both concentrations, concluding that the MS medium with half concentrations of nutrients was considered the most viable for the in vitro establishment of this species. |
publishDate |
2020 |
dc.date.none.fl_str_mv |
2020-10-29 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
https://rsdjournal.org/index.php/rsd/article/view/9378 10.33448/rsd-v9i10.9378 |
url |
https://rsdjournal.org/index.php/rsd/article/view/9378 |
identifier_str_mv |
10.33448/rsd-v9i10.9378 |
dc.language.iso.fl_str_mv |
por |
language |
por |
dc.relation.none.fl_str_mv |
https://rsdjournal.org/index.php/rsd/article/view/9378/8421 |
dc.rights.driver.fl_str_mv |
https://creativecommons.org/licenses/by/4.0 info:eu-repo/semantics/openAccess |
rights_invalid_str_mv |
https://creativecommons.org/licenses/by/4.0 |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Research, Society and Development |
publisher.none.fl_str_mv |
Research, Society and Development |
dc.source.none.fl_str_mv |
Research, Society and Development; Vol. 9 No. 10; e9689109378 Research, Society and Development; Vol. 9 Núm. 10; e9689109378 Research, Society and Development; v. 9 n. 10; e9689109378 2525-3409 reponame:Research, Society and Development instname:Universidade Federal de Itajubá (UNIFEI) instacron:UNIFEI |
instname_str |
Universidade Federal de Itajubá (UNIFEI) |
instacron_str |
UNIFEI |
institution |
UNIFEI |
reponame_str |
Research, Society and Development |
collection |
Research, Society and Development |
repository.name.fl_str_mv |
Research, Society and Development - Universidade Federal de Itajubá (UNIFEI) |
repository.mail.fl_str_mv |
rsd.articles@gmail.com |
_version_ |
1797052662063038464 |