Leishmanicidal activity of Aspidosperma nitidum Benth. Ex Müll. Arg
Autor(a) principal: | |
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Data de Publicação: | 2021 |
Outros Autores: | , , , , |
Tipo de documento: | Artigo |
Idioma: | por |
Título da fonte: | Research, Society and Development |
Texto Completo: | https://rsdjournal.org/index.php/rsd/article/view/12646 |
Resumo: | Drug treatment for leishmaniasis can lead to several adverse reactions, and therapy-resistant isolates of Leishmania have already been found. In this scenario, the search for therapeutic alternatives and alkaloids, isolated from plants belonging to the genus Aspidosperma, has proved to be promising as antiparasitic. The present study evaluated the antipromastigote, amastigote and cytotoxicity activity of the extract and fractions belonging to A. nitidum. The ethanol extract of the shells (EE) was obtained by maceration and submitted to an acid:base partition, with the FN and FA fractions being obtained. In addition, the EE was subjected to fractionation under reflux, obtaining the fractions FrHEX, FrDCL, FrAcOEt and FrMeOH. The antipromastigote activity in L. amazonesis and cytotoxicity to macrophages were evaluated using the viability test (MTT). After the invasion of macrophages by the parasite, the treatment was performed with different sample concentrations during 72 hours, followed by samples staining and reading under the optical microscope. Only FrDCL was moderately active against promastigotes (IC50=105.7 µg/mL). However, EE (IC50 = 23.87 µg/mL) and FA (IC50 = 18.5 µg/mL) were shown to be active against amastigote, whereas in FrDCL (IC50 = 204.5 µg/mL) and FrHEX (IC50 = 143.0 µg/mL) moderate activity was observed. When samples cytoxicity is related to antiamastigote activity, the EE (CC50 = 491.8 µg/mL; IS = 21) seems to be more promising than the FA (CC50 = 209.1 µg/mL; IS = 11). In summary, A. nitidum is promise as a leishmanicide. |
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Leishmanicidal activity of Aspidosperma nitidum Benth. Ex Müll. ArgActividad leishmanicida de Aspidosperma nitidum Benth. Ex Müll. ArgAtividade leishmanicida de Aspidosperma nitidum Benth. Ex Müll. ArgAspidospermaLeismaniaExtractoFracción.AspidospermaLeismaniaExtratoFração.AspidospermaLeismaniaExtractFraction.Drug treatment for leishmaniasis can lead to several adverse reactions, and therapy-resistant isolates of Leishmania have already been found. In this scenario, the search for therapeutic alternatives and alkaloids, isolated from plants belonging to the genus Aspidosperma, has proved to be promising as antiparasitic. The present study evaluated the antipromastigote, amastigote and cytotoxicity activity of the extract and fractions belonging to A. nitidum. The ethanol extract of the shells (EE) was obtained by maceration and submitted to an acid:base partition, with the FN and FA fractions being obtained. In addition, the EE was subjected to fractionation under reflux, obtaining the fractions FrHEX, FrDCL, FrAcOEt and FrMeOH. The antipromastigote activity in L. amazonesis and cytotoxicity to macrophages were evaluated using the viability test (MTT). After the invasion of macrophages by the parasite, the treatment was performed with different sample concentrations during 72 hours, followed by samples staining and reading under the optical microscope. Only FrDCL was moderately active against promastigotes (IC50=105.7 µg/mL). However, EE (IC50 = 23.87 µg/mL) and FA (IC50 = 18.5 µg/mL) were shown to be active against amastigote, whereas in FrDCL (IC50 = 204.5 µg/mL) and FrHEX (IC50 = 143.0 µg/mL) moderate activity was observed. When samples cytoxicity is related to antiamastigote activity, the EE (CC50 = 491.8 µg/mL; IS = 21) seems to be more promising than the FA (CC50 = 209.1 µg/mL; IS = 11). In summary, A. nitidum is promise as a leishmanicide.El tratamiento farmacológico de la leishmaniasis puede provocar varias reacciones adversas y ya se han encontrado cepas de Leishmania resistentes al tratamiento. En este escenario, la búsqueda de alternativas terapéuticas y alcaloides, aislados de plantas pertenecientes al género Aspidosperma, ha demostrado ser prometedora como antiparasitario. El presente estudio evaluó la actividad antipromastigota, amastigota y citotoxicidad del extracto y fracciones pertenecientes a A. nitidum. El extracto etanólico de las cáscaras (EE) se obtuvo por maceración y sometido a un reparto ácido: base, obteniéndose las fracciones FN y FA. Además, el EE se sometió a fraccionamiento a reflujo, obteniendo las fracciones FrHEX, FrDCL, FrAcOEt y FrMeOH. La actividad antipromastigota en Leishmania (Leishmania) amazonensis y la citotoxicidad para los macrófagos se evaluaron mediante la prueba de viabilidad (MTT). Tras la invasión de los macrófagos por el parásito, se realizó el tratamiento con diferentes concentraciones de las muestras durante 72 h, seguido de la fijación y tinción de las muestras y lectura al microscopio óptico. Sólo FrDCL fue moderadamente activo contra promastigote (CI50 = 105,7 ± 1,12 µg / ml). Sin embargo, EE (CI50 = 23,87 ± 0,87 µg / mL) y FA (CI50 = 18,5 ± 0,94 µg / mL) mostraron ser activos contra amastigote, mientras que en FrDCL (CI50 = 204, 5 ± 0,71 µg / mL) y FrHEX (CI50 = 143,0 ± 0,82 µg / ml) se observó una actividad moderada. Cuando la citotoxicidad de las muestras se relaciona con la actividad antiamastigota, se observa que el EE (CC50 = 491,8 + 1,86 µg / mL; IS = 21) parece ser más prometedor que el FA (CC50 = 209,1 + 1, 7 µg / mL; IS = 11). En resumen, A. nitidum se ha mostrado prometedor como leishmanicida.O tratamento medicamentoso da leishmaniose pode acarretar várias reações adversas e já foram encontrados isolados de Leishmania resistentes a terapêutica. Neste cenário, torna-se imprescindível a busca de alternativas terapêuticas e alcaloides, isolados de plantas pertencentes ao gênero Aspidosperma, têm se mostrado promissores como antiparasitário. O presente estudo avaliou a atividade antipromastigota, amastigota e citotoxicidade do extrato e frações pertencentes a A. nitidum. O extrato etanólico das cascas (EE) foi obtido por maceração e submetido a partição ácido:base, sendo obtidas as frações FN e FA. Além disso, o EE foi submetido ao fracionamento sob refluxo, obtendo-se as frações FrHEX, FrDCL, FrAcOEt e FrMeOH. A atividade antipromastigota em L. amazonensis e citotoxicidade para macrófagos foram avaliadas através do ensaio de viabilidade (MTT). Após a invasão dos macrófagos pelo parasito, realizou-se o tratamento com diferentes concentrações das amostras por 72 h, seguida de fixação e coloração das amostras e leitura em microscópio óptico. Somente a FrDCL mostrou-se moderadamente ativa contra promastigota (CI50= 105,7±1,12 µg/mL). No entanto, o EE (CI50= 23,87 ±0,87 µg/mL) e FA (CI50= 18,5 ±0,94 µg/mL) mostraram-se ativos contra amastigota, enquanto que na FrDCL (CI50= 204,5 ± 0,71 µg/mL) e FrHEX (CI50= 143,0 ±0,82 µg/mL) observou-se atividade moderada. Quando se relaciona a citotoxicidade das amostras a atividade antiamastigota, observa-se que o EE (CC50= 491,8 + 1,86 µg/mL; IS= 21) parece ser mais promissor que a FA (CC50= 209,1 + 1,7 µg/mL; IS=11). Em síntese, a A. nitidum mostrou-se promissora como leishmanicida.Research, Society and Development2021-02-26info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://rsdjournal.org/index.php/rsd/article/view/1264610.33448/rsd-v10i2.12646Research, Society and Development; Vol. 10 No. 2; e50210212646Research, Society and Development; Vol. 10 Núm. 2; e50210212646Research, Society and Development; v. 10 n. 2; e502102126462525-3409reponame:Research, Society and Developmentinstname:Universidade Federal de Itajubá (UNIFEI)instacron:UNIFEIporhttps://rsdjournal.org/index.php/rsd/article/view/12646/11539Copyright (c) 2021 Andreza do Socorro Silva da Veiga; Fernando Tobias Silveira; Andrey Moacir do Rosario Marinho; Rafaela Cabral dos Santos da Trindade; Marliane Batista Campos; Maria Fani Dolabelahttps://creativecommons.org/licenses/by/4.0info:eu-repo/semantics/openAccessVeiga, Andreza do Socorro Silva da Silveira, Fernando Tobias Marinho, Andrey Moacir do Rosario Trindade, Rafaela Cabral dos Santos da Campos, Marliane Batista Dolabela, Maria Fani 2021-03-02T09:32:39Zoai:ojs.pkp.sfu.ca:article/12646Revistahttps://rsdjournal.org/index.php/rsd/indexPUBhttps://rsdjournal.org/index.php/rsd/oairsd.articles@gmail.com2525-34092525-3409opendoar:2024-01-17T09:34:09.892392Research, Society and Development - Universidade Federal de Itajubá (UNIFEI)false |
dc.title.none.fl_str_mv |
Leishmanicidal activity of Aspidosperma nitidum Benth. Ex Müll. Arg Actividad leishmanicida de Aspidosperma nitidum Benth. Ex Müll. Arg Atividade leishmanicida de Aspidosperma nitidum Benth. Ex Müll. Arg |
title |
Leishmanicidal activity of Aspidosperma nitidum Benth. Ex Müll. Arg |
spellingShingle |
Leishmanicidal activity of Aspidosperma nitidum Benth. Ex Müll. Arg Veiga, Andreza do Socorro Silva da Aspidosperma Leismania Extracto Fracción. Aspidosperma Leismania Extrato Fração. Aspidosperma Leismania Extract Fraction. |
title_short |
Leishmanicidal activity of Aspidosperma nitidum Benth. Ex Müll. Arg |
title_full |
Leishmanicidal activity of Aspidosperma nitidum Benth. Ex Müll. Arg |
title_fullStr |
Leishmanicidal activity of Aspidosperma nitidum Benth. Ex Müll. Arg |
title_full_unstemmed |
Leishmanicidal activity of Aspidosperma nitidum Benth. Ex Müll. Arg |
title_sort |
Leishmanicidal activity of Aspidosperma nitidum Benth. Ex Müll. Arg |
author |
Veiga, Andreza do Socorro Silva da |
author_facet |
Veiga, Andreza do Socorro Silva da Silveira, Fernando Tobias Marinho, Andrey Moacir do Rosario Trindade, Rafaela Cabral dos Santos da Campos, Marliane Batista Dolabela, Maria Fani |
author_role |
author |
author2 |
Silveira, Fernando Tobias Marinho, Andrey Moacir do Rosario Trindade, Rafaela Cabral dos Santos da Campos, Marliane Batista Dolabela, Maria Fani |
author2_role |
author author author author author |
dc.contributor.author.fl_str_mv |
Veiga, Andreza do Socorro Silva da Silveira, Fernando Tobias Marinho, Andrey Moacir do Rosario Trindade, Rafaela Cabral dos Santos da Campos, Marliane Batista Dolabela, Maria Fani |
dc.subject.por.fl_str_mv |
Aspidosperma Leismania Extracto Fracción. Aspidosperma Leismania Extrato Fração. Aspidosperma Leismania Extract Fraction. |
topic |
Aspidosperma Leismania Extracto Fracción. Aspidosperma Leismania Extrato Fração. Aspidosperma Leismania Extract Fraction. |
description |
Drug treatment for leishmaniasis can lead to several adverse reactions, and therapy-resistant isolates of Leishmania have already been found. In this scenario, the search for therapeutic alternatives and alkaloids, isolated from plants belonging to the genus Aspidosperma, has proved to be promising as antiparasitic. The present study evaluated the antipromastigote, amastigote and cytotoxicity activity of the extract and fractions belonging to A. nitidum. The ethanol extract of the shells (EE) was obtained by maceration and submitted to an acid:base partition, with the FN and FA fractions being obtained. In addition, the EE was subjected to fractionation under reflux, obtaining the fractions FrHEX, FrDCL, FrAcOEt and FrMeOH. The antipromastigote activity in L. amazonesis and cytotoxicity to macrophages were evaluated using the viability test (MTT). After the invasion of macrophages by the parasite, the treatment was performed with different sample concentrations during 72 hours, followed by samples staining and reading under the optical microscope. Only FrDCL was moderately active against promastigotes (IC50=105.7 µg/mL). However, EE (IC50 = 23.87 µg/mL) and FA (IC50 = 18.5 µg/mL) were shown to be active against amastigote, whereas in FrDCL (IC50 = 204.5 µg/mL) and FrHEX (IC50 = 143.0 µg/mL) moderate activity was observed. When samples cytoxicity is related to antiamastigote activity, the EE (CC50 = 491.8 µg/mL; IS = 21) seems to be more promising than the FA (CC50 = 209.1 µg/mL; IS = 11). In summary, A. nitidum is promise as a leishmanicide. |
publishDate |
2021 |
dc.date.none.fl_str_mv |
2021-02-26 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
https://rsdjournal.org/index.php/rsd/article/view/12646 10.33448/rsd-v10i2.12646 |
url |
https://rsdjournal.org/index.php/rsd/article/view/12646 |
identifier_str_mv |
10.33448/rsd-v10i2.12646 |
dc.language.iso.fl_str_mv |
por |
language |
por |
dc.relation.none.fl_str_mv |
https://rsdjournal.org/index.php/rsd/article/view/12646/11539 |
dc.rights.driver.fl_str_mv |
https://creativecommons.org/licenses/by/4.0 info:eu-repo/semantics/openAccess |
rights_invalid_str_mv |
https://creativecommons.org/licenses/by/4.0 |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Research, Society and Development |
publisher.none.fl_str_mv |
Research, Society and Development |
dc.source.none.fl_str_mv |
Research, Society and Development; Vol. 10 No. 2; e50210212646 Research, Society and Development; Vol. 10 Núm. 2; e50210212646 Research, Society and Development; v. 10 n. 2; e50210212646 2525-3409 reponame:Research, Society and Development instname:Universidade Federal de Itajubá (UNIFEI) instacron:UNIFEI |
instname_str |
Universidade Federal de Itajubá (UNIFEI) |
instacron_str |
UNIFEI |
institution |
UNIFEI |
reponame_str |
Research, Society and Development |
collection |
Research, Society and Development |
repository.name.fl_str_mv |
Research, Society and Development - Universidade Federal de Itajubá (UNIFEI) |
repository.mail.fl_str_mv |
rsd.articles@gmail.com |
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1797052745716334592 |