Lactose quantification in UHT milk by high-performance liquid chromatography and cryoscopy (freezing point depression)
Autor(a) principal: | |
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Data de Publicação: | 2021 |
Outros Autores: | , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Research, Society and Development |
Texto Completo: | https://rsdjournal.org/index.php/rsd/article/view/23224 |
Resumo: | Due to the large number of people with lactose maldigestion, the dairy industries have increased production and diversity of low lactose and lactose-free foods. Consequently, the need to control the lactose hydrolysis process has also risen. This study aimed to correlate freezing point depression (cryoscopy) and lactose concentration, quantified by high-performance liquid chromatography (HPLC), in UHT milk. To accomplish this, UHT milk samples were subjected to seven lactose hydrolysis treatments, using lactase enzyme, resulting in different lactose concentrations. All samples were subjected to HPLC analysis and freezing point measurement, using a cryoscope. The results were plotted on a graph and a linear regression was performed. There was a strong correlation between lactose concentration and freezing point (R = 0,9973) and the coefficient of determination (R2) was 0,9946, which means that 99,46% of the variability of the response data is explained by the linear regression model. Therefore, the results point to the feasibility of estimating the lactose concentration in milk during the hydrolysis process for the production of low lactose milk, by cryoscopy, a quick analysis, with lower cost compared to HPLC and that is already among the analyses commonly performed in dairy industries. |
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Lactose quantification in UHT milk by high-performance liquid chromatography and cryoscopy (freezing point depression)Cuantificación de lactosa en leche UHT mediante cromatografía líquida de alta resolución y crioscopia (depresión del punto de congelación)Quantificação de lactose em leite UHT por cromatografia líquida de alta eficiência e crioscopia (depressão do ponto de congelamento)MilkLecheβ-galactosidaseCero lactosaMala digestión de lactosa.Leiteβ-galactosidaseZero LactoseMá digestão de lactose.Due to the large number of people with lactose maldigestion, the dairy industries have increased production and diversity of low lactose and lactose-free foods. Consequently, the need to control the lactose hydrolysis process has also risen. This study aimed to correlate freezing point depression (cryoscopy) and lactose concentration, quantified by high-performance liquid chromatography (HPLC), in UHT milk. To accomplish this, UHT milk samples were subjected to seven lactose hydrolysis treatments, using lactase enzyme, resulting in different lactose concentrations. All samples were subjected to HPLC analysis and freezing point measurement, using a cryoscope. The results were plotted on a graph and a linear regression was performed. There was a strong correlation between lactose concentration and freezing point (R = 0,9973) and the coefficient of determination (R2) was 0,9946, which means that 99,46% of the variability of the response data is explained by the linear regression model. Therefore, the results point to the feasibility of estimating the lactose concentration in milk during the hydrolysis process for the production of low lactose milk, by cryoscopy, a quick analysis, with lower cost compared to HPLC and that is already among the analyses commonly performed in dairy industries.Debido a la gran cantidad de personas con mala digestión de lactosa, las industrias lácteas han aumentado la producción y diversidad de alimentos con bajo contenido de lactosa y alimentos sin lactosa. En consecuencia, también ha aumentado la necesidad de controlar el proceso de hidrólisis de lactosa. Este estudio tuvo como objetivo correlacionar la depresión del punto de congelación (crioscopia) y la concentración de lactosa, cuantificada por cromatografía líquida de alta resolución (CLAR), en leche UHT. Para ello, las muestras de leche UHT fueron sometidas a siete tratamientos de hidrólisis de lactosa, utilizando la enzima lactasa, dando como resultado diferentes concentraciones de lactosa. Las muestras se sometieron a análisis por CLAR y determinación del punto de congelación, usando un crioscopio. Los resultados se representaron en un gráfico y se realizó una regresión lineal. Hubo una fuerte correlación entre la concentración de lactosa y el punto de congelación (R = 0,9973) y el coeficiente de determinación (R2) fue 0,9946, lo que significa que el 99,46% de la variabilidad de los datos de respuesta se explica por el modelo de regresión lineal. Por tanto, los resultados apuntan a la viabilidad de estimar la concentración de lactosa en la leche durante el proceso de hidrólisis para la producción de leche con bajo contenido en lactosa, mediante crioscopia, un análisis rápido, con menor costo en comparación con el CLAR y que ya está entre los análisis comúnmente realizado en industrias lácteas.Devido ao grande número de pessoas com má digestão de lactose, as indústrias de laticínios aumentaram a produção e a diversidade de alimentos com baixo teor de lactose e sem lactose. Consequentemente, a necessidade de controlar o processo de hidrólise da lactose também aumentou. Este estudo teve como objetivo correlacionar depressão de ponto de congelamento (crioscopia) e concentração de lactose, quantificada por cromatografia líquida de alta eficiência (CLAE), em leite UHT. Para isso, amostras de leite UHT foram submetidas a sete tratamentos de hidrólise da lactose, utilizando a enzima lactase, resultando em diferentes concentrações de lactose. As amostras foram submetidas a análises de CLAE e determinação de ponto de congelamento, utilizando um crioscópio. Os resultados foram plotados em um gráfico e uma regressão linear foi realizada. Houve uma forte correlação entre concentração de lactose e ponto de congelamento (R = 0,9973) e o coeficiente de determinação (R2) foi de 0,9946, o que significa que 99,46% da variabilidade dos dados de resposta são explicados pelo modelo da regressão linear. Portanto, os resultados apontam para a viabilidade de estimar a concentração de lactose no leite durante o processo de hidrólise para produção de leite com baixo teor de lactose, por meio da crioscopia, uma análise rápida, com menor custo em relação à análise de CLAE e que já se encontra entre as análises comumente realizadas em indústrias de laticínios.Research, Society and Development2021-11-23info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://rsdjournal.org/index.php/rsd/article/view/2322410.33448/rsd-v10i15.23224Research, Society and Development; Vol. 10 No. 15; e454101523224Research, Society and Development; Vol. 10 Núm. 15; e454101523224Research, Society and Development; v. 10 n. 15; e4541015232242525-3409reponame:Research, Society and Developmentinstname:Universidade Federal de Itajubá (UNIFEI)instacron:UNIFEIenghttps://rsdjournal.org/index.php/rsd/article/view/23224/20181Copyright (c) 2021 Cleiver Júnio Martins Costa; Camila Alves Moreira; Ricardo Corrêa de Santana; Amado Jésus Silva; Juliana Karla de Souza Teixeira Almeida; Milla Gabriela dos Santoshttps://creativecommons.org/licenses/by/4.0info:eu-repo/semantics/openAccessCosta, Cleiver Júnio MartinsMoreira, Camila AlvesSantana, Ricardo Corrêa deSilva, Amado Jésus Almeida, Juliana Karla de Souza Teixeira Santos, Milla Gabriela dos 2021-12-06T10:13:53Zoai:ojs.pkp.sfu.ca:article/23224Revistahttps://rsdjournal.org/index.php/rsd/indexPUBhttps://rsdjournal.org/index.php/rsd/oairsd.articles@gmail.com2525-34092525-3409opendoar:2024-01-17T09:42:09.707776Research, Society and Development - Universidade Federal de Itajubá (UNIFEI)false |
dc.title.none.fl_str_mv |
Lactose quantification in UHT milk by high-performance liquid chromatography and cryoscopy (freezing point depression) Cuantificación de lactosa en leche UHT mediante cromatografía líquida de alta resolución y crioscopia (depresión del punto de congelación) Quantificação de lactose em leite UHT por cromatografia líquida de alta eficiência e crioscopia (depressão do ponto de congelamento) |
title |
Lactose quantification in UHT milk by high-performance liquid chromatography and cryoscopy (freezing point depression) |
spellingShingle |
Lactose quantification in UHT milk by high-performance liquid chromatography and cryoscopy (freezing point depression) Costa, Cleiver Júnio Martins Milk Leche β-galactosidase Cero lactosa Mala digestión de lactosa. Leite β-galactosidase Zero Lactose Má digestão de lactose. |
title_short |
Lactose quantification in UHT milk by high-performance liquid chromatography and cryoscopy (freezing point depression) |
title_full |
Lactose quantification in UHT milk by high-performance liquid chromatography and cryoscopy (freezing point depression) |
title_fullStr |
Lactose quantification in UHT milk by high-performance liquid chromatography and cryoscopy (freezing point depression) |
title_full_unstemmed |
Lactose quantification in UHT milk by high-performance liquid chromatography and cryoscopy (freezing point depression) |
title_sort |
Lactose quantification in UHT milk by high-performance liquid chromatography and cryoscopy (freezing point depression) |
author |
Costa, Cleiver Júnio Martins |
author_facet |
Costa, Cleiver Júnio Martins Moreira, Camila Alves Santana, Ricardo Corrêa de Silva, Amado Jésus Almeida, Juliana Karla de Souza Teixeira Santos, Milla Gabriela dos |
author_role |
author |
author2 |
Moreira, Camila Alves Santana, Ricardo Corrêa de Silva, Amado Jésus Almeida, Juliana Karla de Souza Teixeira Santos, Milla Gabriela dos |
author2_role |
author author author author author |
dc.contributor.author.fl_str_mv |
Costa, Cleiver Júnio Martins Moreira, Camila Alves Santana, Ricardo Corrêa de Silva, Amado Jésus Almeida, Juliana Karla de Souza Teixeira Santos, Milla Gabriela dos |
dc.subject.por.fl_str_mv |
Milk Leche β-galactosidase Cero lactosa Mala digestión de lactosa. Leite β-galactosidase Zero Lactose Má digestão de lactose. |
topic |
Milk Leche β-galactosidase Cero lactosa Mala digestión de lactosa. Leite β-galactosidase Zero Lactose Má digestão de lactose. |
description |
Due to the large number of people with lactose maldigestion, the dairy industries have increased production and diversity of low lactose and lactose-free foods. Consequently, the need to control the lactose hydrolysis process has also risen. This study aimed to correlate freezing point depression (cryoscopy) and lactose concentration, quantified by high-performance liquid chromatography (HPLC), in UHT milk. To accomplish this, UHT milk samples were subjected to seven lactose hydrolysis treatments, using lactase enzyme, resulting in different lactose concentrations. All samples were subjected to HPLC analysis and freezing point measurement, using a cryoscope. The results were plotted on a graph and a linear regression was performed. There was a strong correlation between lactose concentration and freezing point (R = 0,9973) and the coefficient of determination (R2) was 0,9946, which means that 99,46% of the variability of the response data is explained by the linear regression model. Therefore, the results point to the feasibility of estimating the lactose concentration in milk during the hydrolysis process for the production of low lactose milk, by cryoscopy, a quick analysis, with lower cost compared to HPLC and that is already among the analyses commonly performed in dairy industries. |
publishDate |
2021 |
dc.date.none.fl_str_mv |
2021-11-23 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
https://rsdjournal.org/index.php/rsd/article/view/23224 10.33448/rsd-v10i15.23224 |
url |
https://rsdjournal.org/index.php/rsd/article/view/23224 |
identifier_str_mv |
10.33448/rsd-v10i15.23224 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
https://rsdjournal.org/index.php/rsd/article/view/23224/20181 |
dc.rights.driver.fl_str_mv |
https://creativecommons.org/licenses/by/4.0 info:eu-repo/semantics/openAccess |
rights_invalid_str_mv |
https://creativecommons.org/licenses/by/4.0 |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Research, Society and Development |
publisher.none.fl_str_mv |
Research, Society and Development |
dc.source.none.fl_str_mv |
Research, Society and Development; Vol. 10 No. 15; e454101523224 Research, Society and Development; Vol. 10 Núm. 15; e454101523224 Research, Society and Development; v. 10 n. 15; e454101523224 2525-3409 reponame:Research, Society and Development instname:Universidade Federal de Itajubá (UNIFEI) instacron:UNIFEI |
instname_str |
Universidade Federal de Itajubá (UNIFEI) |
instacron_str |
UNIFEI |
institution |
UNIFEI |
reponame_str |
Research, Society and Development |
collection |
Research, Society and Development |
repository.name.fl_str_mv |
Research, Society and Development - Universidade Federal de Itajubá (UNIFEI) |
repository.mail.fl_str_mv |
rsd.articles@gmail.com |
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1797052809696247808 |