Effect of serial photodynamic therapy with curcumin on Leishmania braziliensis and Leishmania amazonensis promastigotes
Autor(a) principal: | |
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Data de Publicação: | 2021 |
Outros Autores: | , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Research, Society and Development |
Texto Completo: | https://rsdjournal.org/index.php/rsd/article/view/19544 |
Resumo: | Photodynamic Therapy (PDT) consists of using a light source and a photosensitive drug at an appropriate wavelength and molecular oxygen to trigger cell death through the production of reactive oxygen species. Because it is a localised therapy, PDT is shown to be ideal for skin diseases. American cutaneous Leishmaniasis (ACL) is a highly prevalent protozoan disease worldwide that presents different clinical evolutions and may result in ulcerations and disfiguring lesions on the skin and cartilage. This study was aimed at evaluating the effect in vitro of PDT applied serially using curcumin as a photosensitiser. For this, a concentration of 125 µg.mL-1 of curcumin was used on Leishmania braziliensis and Leishmania amazonensis strains, with a light fluence of 10 J.cm-2 and irradiance of 110 mW.cm-2. The tests done were viability analysis by trypan blue exclusion test, analysis of photosensitizer (PS) internalization by confocal microscopy and morphological alterations by May-Grunwald/Giemsa staining. We observed that there was internalisation of the PS before the first and second application of PDT, with L. braziliensis and L. amazonensis strains mortality of 92% and 82% respectively, after the second application, and induction of alterations in the structural conformation, such as cell size and non-evidence of nucleus and flagellum, demonstrating that PDT was effective. We conclude that serial PDT was effective in inducing the mortality of promastigotes forms of L. braziliensis and L. amazonensis in vitro, thus highlighting its potential for the treatment of leishmaniasis. |
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Effect of serial photodynamic therapy with curcumin on Leishmania braziliensis and Leishmania amazonensis promastigotesEfecto de la fotodinámica serie con curcumina sobre Leishmania braziliensis y Leishmania amazonensis promastigotosEfeito da terapia fotodinâmica seriada com curcumina em promastigotas de Leishmania braziliensis e Leishmania amazonensisTerapia fotodinâmicaLeishmaniose Cutânea AmericanaCurcumina.Terapia fotodinámicaLeishmaniasis Cutánea AmericanaCurcumina.Photodynamic therapyAmerican Cutaneous LeishmaniasisCurcumin.Photodynamic Therapy (PDT) consists of using a light source and a photosensitive drug at an appropriate wavelength and molecular oxygen to trigger cell death through the production of reactive oxygen species. Because it is a localised therapy, PDT is shown to be ideal for skin diseases. American cutaneous Leishmaniasis (ACL) is a highly prevalent protozoan disease worldwide that presents different clinical evolutions and may result in ulcerations and disfiguring lesions on the skin and cartilage. This study was aimed at evaluating the effect in vitro of PDT applied serially using curcumin as a photosensitiser. For this, a concentration of 125 µg.mL-1 of curcumin was used on Leishmania braziliensis and Leishmania amazonensis strains, with a light fluence of 10 J.cm-2 and irradiance of 110 mW.cm-2. The tests done were viability analysis by trypan blue exclusion test, analysis of photosensitizer (PS) internalization by confocal microscopy and morphological alterations by May-Grunwald/Giemsa staining. We observed that there was internalisation of the PS before the first and second application of PDT, with L. braziliensis and L. amazonensis strains mortality of 92% and 82% respectively, after the second application, and induction of alterations in the structural conformation, such as cell size and non-evidence of nucleus and flagellum, demonstrating that PDT was effective. We conclude that serial PDT was effective in inducing the mortality of promastigotes forms of L. braziliensis and L. amazonensis in vitro, thus highlighting its potential for the treatment of leishmaniasis.La terapia fotodinámica (TFD) consiste en utilizar una fuente de luz y un fármaco fotosensible a una longitud de onda adecuada y oxígeno molecular para desencadenar la muerte celular mediante la producción de especies reactivas de oxígeno. Debido a que es una terapia localizada, se ha demostrado que la TFD es ideal para las enfermedades de la piel. La leishmaniasis Cutánea Americana (LCA) es una enfermedad protozoaria de alta prevalencia a nivel mundial que presenta diferentes evoluciones clínicas y puede resultar en ulceraciones y lesiones desfigurantes en la piel y cartílago. Este estudio tuvo como objetivo evaluar el efecto in vitro de la TFD aplicada en serie utilizando curcumina como fotosensibilizador. Para ello, se utilizó una concentración de 125 µg.mL-1 de curcumina en cepas de Leishmania braziliensis y Leishmania amazonensis, con una fluencia de luz de 10 J.cm-² e irradiancia de 110 mW.cm‑². Las pruebas realizadas fueron análisis de viabilidad mediante prueba de exclusión con azul tripán, análisis de la internalización del fotosensibilizador (FS) por microscopía confocal y alteraciones morfológicas mediante tinción de May-Grunwald/Giemsa. Observamos que hubo internalización del FS antes de la primera y segunda aplicación de TFD, con una mortalidad de las cepas de L. braziliensis y L. amazonensis del 92% y 82% respectivamente, después de la segunda aplicación, e inducción de alteraciones en la conformación estructural, como el tamaño de la célula y la ausencia de evidencia de núcleo y flagelo, lo que demuestra que la TFD fue eficaz. Concluimos que la TFD en serie fue eficaz para inducir la mortalidad de formas promastigotes de L. braziliensis y L. amazonensis in vitro, destacando así su potencial para el tratamiento de la leishmaniasis.A terapia fotodinâmica (TFD) consiste no uso de uma fonte de luz e um fármaco fotossensível em um comprimento de onda adequado e oxigênio molecular para desencadear a morte celular por meio da produção de espécies reativas de oxigênio. Por ser uma terapia localizada, a TFD se mostra ideal para doenças de pele. A Leishmaniose Cutânea Americana (LCA) é uma doença protozoária de alta prevalência em todo o mundo que apresenta diferentes evoluções clínicas, podendo resultar em ulcerações e lesões desfigurantes na pele e cartilagem. Este estudo teve como objetivo avaliar o efeito in vitro da TFD aplicada em série usando a curcumina como fotossensibilizador. Para isso, foi utilizada uma concentração de 125 µg.mL-1 de curcumina nas cepas de Leishmania braziliensis e Leishmania amazonensis, com fluência de luz de 10 J.cm-² e irradiância de 110 mW.cm-². Os testes realizados foram análise de viabilidade por teste de exclusão de azul de tripan, análise de internalização do fotossensibilizador (FS) por Microscopia Confocal e alterações morfológicas por coloração de May-Grunwald/ Giemsa. Observamos que houve internalização do FS antes da primeira e segunda aplicação da TFD, com mortalidade das cepas de L. braziliensis e L. amazonensis de 92% e 82% respectivamente, após a segunda aplicação, e indução de alterações na conformação estrutural, como tamanho da célula e não evidência de núcleo e flagelo, demonstrando que a TFD foi eficaz. Concluímos que a TFD seriada foi eficaz em induzir a mortalidade das formas promastigotas de L. braziliensis e L. amazonensis in vitro, destacando seu potencial para o tratamento da leishmaniose.Research, Society and Development2021-08-29info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://rsdjournal.org/index.php/rsd/article/view/1954410.33448/rsd-v10i11.19544Research, Society and Development; Vol. 10 No. 11; e219101119544Research, Society and Development; Vol. 10 Núm. 11; e219101119544Research, Society and Development; v. 10 n. 11; e2191011195442525-3409reponame:Research, Society and Developmentinstname:Universidade Federal de Itajubá (UNIFEI)instacron:UNIFEIenghttps://rsdjournal.org/index.php/rsd/article/view/19544/17575Copyright (c) 2021 Lucas Tobias Rodrigues Maciel; Luciana Maria Cortez Marcolino; Fernanda Bueno Sant’Anna Pereira Maciel; Juliana Guerra Pinto; Juliana Ferreira-Strixinohttps://creativecommons.org/licenses/by/4.0info:eu-repo/semantics/openAccessMaciel, Lucas Tobias RodriguesMarcolino, Luciana Maria CortezMaciel, Fernanda Bueno Sant’Anna Pereira Pinto, Juliana GuerraFerreira-Strixino, Juliana2021-10-23T19:01:11Zoai:ojs.pkp.sfu.ca:article/19544Revistahttps://rsdjournal.org/index.php/rsd/indexPUBhttps://rsdjournal.org/index.php/rsd/oairsd.articles@gmail.com2525-34092525-3409opendoar:2024-01-17T09:39:24.736752Research, Society and Development - Universidade Federal de Itajubá (UNIFEI)false |
dc.title.none.fl_str_mv |
Effect of serial photodynamic therapy with curcumin on Leishmania braziliensis and Leishmania amazonensis promastigotes Efecto de la fotodinámica serie con curcumina sobre Leishmania braziliensis y Leishmania amazonensis promastigotos Efeito da terapia fotodinâmica seriada com curcumina em promastigotas de Leishmania braziliensis e Leishmania amazonensis |
title |
Effect of serial photodynamic therapy with curcumin on Leishmania braziliensis and Leishmania amazonensis promastigotes |
spellingShingle |
Effect of serial photodynamic therapy with curcumin on Leishmania braziliensis and Leishmania amazonensis promastigotes Maciel, Lucas Tobias Rodrigues Terapia fotodinâmica Leishmaniose Cutânea Americana Curcumina. Terapia fotodinámica Leishmaniasis Cutánea Americana Curcumina. Photodynamic therapy American Cutaneous Leishmaniasis Curcumin. |
title_short |
Effect of serial photodynamic therapy with curcumin on Leishmania braziliensis and Leishmania amazonensis promastigotes |
title_full |
Effect of serial photodynamic therapy with curcumin on Leishmania braziliensis and Leishmania amazonensis promastigotes |
title_fullStr |
Effect of serial photodynamic therapy with curcumin on Leishmania braziliensis and Leishmania amazonensis promastigotes |
title_full_unstemmed |
Effect of serial photodynamic therapy with curcumin on Leishmania braziliensis and Leishmania amazonensis promastigotes |
title_sort |
Effect of serial photodynamic therapy with curcumin on Leishmania braziliensis and Leishmania amazonensis promastigotes |
author |
Maciel, Lucas Tobias Rodrigues |
author_facet |
Maciel, Lucas Tobias Rodrigues Marcolino, Luciana Maria Cortez Maciel, Fernanda Bueno Sant’Anna Pereira Pinto, Juliana Guerra Ferreira-Strixino, Juliana |
author_role |
author |
author2 |
Marcolino, Luciana Maria Cortez Maciel, Fernanda Bueno Sant’Anna Pereira Pinto, Juliana Guerra Ferreira-Strixino, Juliana |
author2_role |
author author author author |
dc.contributor.author.fl_str_mv |
Maciel, Lucas Tobias Rodrigues Marcolino, Luciana Maria Cortez Maciel, Fernanda Bueno Sant’Anna Pereira Pinto, Juliana Guerra Ferreira-Strixino, Juliana |
dc.subject.por.fl_str_mv |
Terapia fotodinâmica Leishmaniose Cutânea Americana Curcumina. Terapia fotodinámica Leishmaniasis Cutánea Americana Curcumina. Photodynamic therapy American Cutaneous Leishmaniasis Curcumin. |
topic |
Terapia fotodinâmica Leishmaniose Cutânea Americana Curcumina. Terapia fotodinámica Leishmaniasis Cutánea Americana Curcumina. Photodynamic therapy American Cutaneous Leishmaniasis Curcumin. |
description |
Photodynamic Therapy (PDT) consists of using a light source and a photosensitive drug at an appropriate wavelength and molecular oxygen to trigger cell death through the production of reactive oxygen species. Because it is a localised therapy, PDT is shown to be ideal for skin diseases. American cutaneous Leishmaniasis (ACL) is a highly prevalent protozoan disease worldwide that presents different clinical evolutions and may result in ulcerations and disfiguring lesions on the skin and cartilage. This study was aimed at evaluating the effect in vitro of PDT applied serially using curcumin as a photosensitiser. For this, a concentration of 125 µg.mL-1 of curcumin was used on Leishmania braziliensis and Leishmania amazonensis strains, with a light fluence of 10 J.cm-2 and irradiance of 110 mW.cm-2. The tests done were viability analysis by trypan blue exclusion test, analysis of photosensitizer (PS) internalization by confocal microscopy and morphological alterations by May-Grunwald/Giemsa staining. We observed that there was internalisation of the PS before the first and second application of PDT, with L. braziliensis and L. amazonensis strains mortality of 92% and 82% respectively, after the second application, and induction of alterations in the structural conformation, such as cell size and non-evidence of nucleus and flagellum, demonstrating that PDT was effective. We conclude that serial PDT was effective in inducing the mortality of promastigotes forms of L. braziliensis and L. amazonensis in vitro, thus highlighting its potential for the treatment of leishmaniasis. |
publishDate |
2021 |
dc.date.none.fl_str_mv |
2021-08-29 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
https://rsdjournal.org/index.php/rsd/article/view/19544 10.33448/rsd-v10i11.19544 |
url |
https://rsdjournal.org/index.php/rsd/article/view/19544 |
identifier_str_mv |
10.33448/rsd-v10i11.19544 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
https://rsdjournal.org/index.php/rsd/article/view/19544/17575 |
dc.rights.driver.fl_str_mv |
https://creativecommons.org/licenses/by/4.0 info:eu-repo/semantics/openAccess |
rights_invalid_str_mv |
https://creativecommons.org/licenses/by/4.0 |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Research, Society and Development |
publisher.none.fl_str_mv |
Research, Society and Development |
dc.source.none.fl_str_mv |
Research, Society and Development; Vol. 10 No. 11; e219101119544 Research, Society and Development; Vol. 10 Núm. 11; e219101119544 Research, Society and Development; v. 10 n. 11; e219101119544 2525-3409 reponame:Research, Society and Development instname:Universidade Federal de Itajubá (UNIFEI) instacron:UNIFEI |
instname_str |
Universidade Federal de Itajubá (UNIFEI) |
instacron_str |
UNIFEI |
institution |
UNIFEI |
reponame_str |
Research, Society and Development |
collection |
Research, Society and Development |
repository.name.fl_str_mv |
Research, Society and Development - Universidade Federal de Itajubá (UNIFEI) |
repository.mail.fl_str_mv |
rsd.articles@gmail.com |
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1797052754487672832 |