Green synthesis of chitosan by Cunninghamella elegans UCP 1306 using sustainable substrates mediated morphological changes

Detalhes bibliográficos
Autor(a) principal: Silva, Ákylla Fernanda Souza
Data de Publicação: 2022
Outros Autores: Souza, Adriana Ferreira de, Pinheiro, Irapuan Oliveira, Campos-Takaki, Galba Maria de
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Research, Society and Development
Texto Completo: https://rsdjournal.org/index.php/rsd/article/view/29387
Resumo: Currently, the commercial production of chitosan takes place through the thermochemical deacetylation of crustacean shells, which process requires the use of chemical substances, such as strong alkaline solutions, which are important sources of environment pollution. We present a method for the production of chitosan by Cunninghamella elegans UCP 1306 using a green metabolic conversion of agro-industrial residues, based on a 22 Factorial Design and describe the main morphological changes observed in the specie. The highest yield of biomass (6.375 g/L) and chitosan (101.7 mg/g) were observed in the assay 2 (Corn steep liquor -CSL 4%, Cassava wastewater-CWW 4%), where the specie presented loose and branched hyphae with the presence of septations, pellets of 0.4 to 0.5 mm and clumps of up to 0.1 mm. Statistical analysis showed that higher concentration of CSL contributed significantly to the growth of the species. The bands determined in Fourier transform infrared spectroscopy confirmed the degree of deacetylation of 84.61% of the chitosan obtained. This research showed that the use of CSL and CWW in association was very promising, being able to be used as sustainable sources of nitrogen and carbon in the production of fungal chitosan with a high degree of deacetylation and can be applied to the industrial process.
id UNIFEI_d8ef168b2a2b71f2db2ed0300ef6be34
oai_identifier_str oai:ojs.pkp.sfu.ca:article/29387
network_acronym_str UNIFEI
network_name_str Research, Society and Development
repository_id_str
spelling Green synthesis of chitosan by Cunninghamella elegans UCP 1306 using sustainable substrates mediated morphological changesSíntesis verde de quitosano por Cunninghamella elegans UCP 1306 utilizando sustratos sostenibles mediados por cambios morfológicosSíntese verde de quitosana por Cunninghamella elegans UCP 1306 usando substratos sustentáveis mediados por mudanças morfológicas Resíduos agroindustriaisBiopolímerosQuitosana fúngicaCunninghamella elegans.Residuos agroindustrialesBiopolímerosQuitosano fúngicoCunninghamella elegans.Agroindustrial wasteBiopolymersFungal ChitosanCunninghamella elegans.Currently, the commercial production of chitosan takes place through the thermochemical deacetylation of crustacean shells, which process requires the use of chemical substances, such as strong alkaline solutions, which are important sources of environment pollution. We present a method for the production of chitosan by Cunninghamella elegans UCP 1306 using a green metabolic conversion of agro-industrial residues, based on a 22 Factorial Design and describe the main morphological changes observed in the specie. The highest yield of biomass (6.375 g/L) and chitosan (101.7 mg/g) were observed in the assay 2 (Corn steep liquor -CSL 4%, Cassava wastewater-CWW 4%), where the specie presented loose and branched hyphae with the presence of septations, pellets of 0.4 to 0.5 mm and clumps of up to 0.1 mm. Statistical analysis showed that higher concentration of CSL contributed significantly to the growth of the species. The bands determined in Fourier transform infrared spectroscopy confirmed the degree of deacetylation of 84.61% of the chitosan obtained. This research showed that the use of CSL and CWW in association was very promising, being able to be used as sustainable sources of nitrogen and carbon in the production of fungal chitosan with a high degree of deacetylation and can be applied to the industrial process.Actualmente, la producción comercial de quitosano se realiza a través del proceso de desacetilación termoquímica de caparazones de crustáceos, cuyo proceso requiere el uso de sustancias químicas, como soluciones alcalinas fuertes, las cuales son fuentes importantes de contaminación para el medio ambiente. El presente estudio presenta un método para la producción de quitosano por la especie Cunninghamella elegans (UCP 1306) utilizando una conversión metabólica verde con residuos agroindustriales, basado en un Diseño Factorial 2² y se describen los principales cambios morfológicos observados en la especie. El mayor rendimiento de biomasa (6,375 g/L) y quitosano (101,7 mg/g) se observó en el ensayo 2 (licor de maceración de maíz- CSL 4% y agua residual de yuca- CWW 4%), donde las especies presentaron predominio de hojas sueltas y ramificadas. hifas con presencia de tabicaciones, gránulos de 0,4 a 0,5 mm y macizos de hasta 0,1 mm. El análisis estadístico mostró que la concentración más alta de CSL contribuyó significativamente al crecimiento de la especie. Las bandas determinadas por espectroscopia infrarroja transformada de Fourier confirmaron el grado de desacetilación del 84,61% del quitosano obtenida. Esta investigación mostró que la asociación de CSL y CWW fue muy prometedora, pudiéndose utilizar como fuentes sostenibles de carbono y nitrógeno en la producción de quitosano fúngico con un alto grado de desacetilación y puede aplicarse al proceso industrial.Atualmente, a produção comercial de quitosana se dá pelo processo de desacetilação termoquímica a partir de carapaças de crustáceos, cujo processo requer o uso de substâncias químicas, como soluções alcalinas fortes, que constituem importantes fontes de poluição para o meio ambiente. O presente estudo apresenta um método para a produção de quitosana pela espécie Cunninghamella elegans (UCP 1306) usando uma conversão metabólica verde com resíduos agroindustrais, baseado em um Planejamento Fatorial 2² e descrevemos as principais alterações morfológicas observadas na espécie. O maior rendimento de biomassa (6,375 g / L) e quitosana (101,7 mg/g) foi observado no ensaio 2 (licor de maceração de milho-CSL 4% e água residual de mandioca- CWW 4%), onde a espécie apresentou predominância de hifas frouxas e ramificadas com presença de septações, pellets de 0,4 a 0,5 mm e clumps de até 0,1 mm. A análise estatística mostrou que a maior concentração de CSL contribuiu significativamente para o crescimento da espécie. As bandas determinadas na espectroscopia no infravermelho com transformada de Fourier confirmaram o grau de desacetilação de 84,61% da quitosana obtida. Esta pesquisa mostrou que a associação de CSL e CWW foi bastante promissora, podendo ser utilizadas como fontes sustentáveis de carbono e nitrogênio na produção de quitosana fúngica com alto grau de desacetilação e pode ser aplicada ao processo industrial.Research, Society and Development2022-05-28info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://rsdjournal.org/index.php/rsd/article/view/2938710.33448/rsd-v11i7.29387Research, Society and Development; Vol. 11 No. 7; e38211729387Research, Society and Development; Vol. 11 Núm. 7; e38211729387Research, Society and Development; v. 11 n. 7; e382117293872525-3409reponame:Research, Society and Developmentinstname:Universidade Federal de Itajubá (UNIFEI)instacron:UNIFEIenghttps://rsdjournal.org/index.php/rsd/article/view/29387/25970Copyright (c) 2022 Ákylla Fernanda Souza Silva; Adriana Ferreira de Souza; Irapuan Oliveira Pinheiro; Galba Maria de Campos-Takakihttps://creativecommons.org/licenses/by/4.0info:eu-repo/semantics/openAccessSilva, Ákylla Fernanda SouzaSouza, Adriana Ferreira dePinheiro, Irapuan Oliveira Campos-Takaki, Galba Maria de 2022-06-06T15:12:05Zoai:ojs.pkp.sfu.ca:article/29387Revistahttps://rsdjournal.org/index.php/rsd/indexPUBhttps://rsdjournal.org/index.php/rsd/oairsd.articles@gmail.com2525-34092525-3409opendoar:2024-01-17T09:46:29.130810Research, Society and Development - Universidade Federal de Itajubá (UNIFEI)false
dc.title.none.fl_str_mv Green synthesis of chitosan by Cunninghamella elegans UCP 1306 using sustainable substrates mediated morphological changes
Síntesis verde de quitosano por Cunninghamella elegans UCP 1306 utilizando sustratos sostenibles mediados por cambios morfológicos
Síntese verde de quitosana por Cunninghamella elegans UCP 1306 usando substratos sustentáveis mediados por mudanças morfológicas
title Green synthesis of chitosan by Cunninghamella elegans UCP 1306 using sustainable substrates mediated morphological changes
spellingShingle Green synthesis of chitosan by Cunninghamella elegans UCP 1306 using sustainable substrates mediated morphological changes
Silva, Ákylla Fernanda Souza
Resíduos agroindustriais
Biopolímeros
Quitosana fúngica
Cunninghamella elegans.
Residuos agroindustriales
Biopolímeros
Quitosano fúngico
Cunninghamella elegans.
Agroindustrial waste
Biopolymers
Fungal Chitosan
Cunninghamella elegans.
title_short Green synthesis of chitosan by Cunninghamella elegans UCP 1306 using sustainable substrates mediated morphological changes
title_full Green synthesis of chitosan by Cunninghamella elegans UCP 1306 using sustainable substrates mediated morphological changes
title_fullStr Green synthesis of chitosan by Cunninghamella elegans UCP 1306 using sustainable substrates mediated morphological changes
title_full_unstemmed Green synthesis of chitosan by Cunninghamella elegans UCP 1306 using sustainable substrates mediated morphological changes
title_sort Green synthesis of chitosan by Cunninghamella elegans UCP 1306 using sustainable substrates mediated morphological changes
author Silva, Ákylla Fernanda Souza
author_facet Silva, Ákylla Fernanda Souza
Souza, Adriana Ferreira de
Pinheiro, Irapuan Oliveira
Campos-Takaki, Galba Maria de
author_role author
author2 Souza, Adriana Ferreira de
Pinheiro, Irapuan Oliveira
Campos-Takaki, Galba Maria de
author2_role author
author
author
dc.contributor.author.fl_str_mv Silva, Ákylla Fernanda Souza
Souza, Adriana Ferreira de
Pinheiro, Irapuan Oliveira
Campos-Takaki, Galba Maria de
dc.subject.por.fl_str_mv Resíduos agroindustriais
Biopolímeros
Quitosana fúngica
Cunninghamella elegans.
Residuos agroindustriales
Biopolímeros
Quitosano fúngico
Cunninghamella elegans.
Agroindustrial waste
Biopolymers
Fungal Chitosan
Cunninghamella elegans.
topic Resíduos agroindustriais
Biopolímeros
Quitosana fúngica
Cunninghamella elegans.
Residuos agroindustriales
Biopolímeros
Quitosano fúngico
Cunninghamella elegans.
Agroindustrial waste
Biopolymers
Fungal Chitosan
Cunninghamella elegans.
description Currently, the commercial production of chitosan takes place through the thermochemical deacetylation of crustacean shells, which process requires the use of chemical substances, such as strong alkaline solutions, which are important sources of environment pollution. We present a method for the production of chitosan by Cunninghamella elegans UCP 1306 using a green metabolic conversion of agro-industrial residues, based on a 22 Factorial Design and describe the main morphological changes observed in the specie. The highest yield of biomass (6.375 g/L) and chitosan (101.7 mg/g) were observed in the assay 2 (Corn steep liquor -CSL 4%, Cassava wastewater-CWW 4%), where the specie presented loose and branched hyphae with the presence of septations, pellets of 0.4 to 0.5 mm and clumps of up to 0.1 mm. Statistical analysis showed that higher concentration of CSL contributed significantly to the growth of the species. The bands determined in Fourier transform infrared spectroscopy confirmed the degree of deacetylation of 84.61% of the chitosan obtained. This research showed that the use of CSL and CWW in association was very promising, being able to be used as sustainable sources of nitrogen and carbon in the production of fungal chitosan with a high degree of deacetylation and can be applied to the industrial process.
publishDate 2022
dc.date.none.fl_str_mv 2022-05-28
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv https://rsdjournal.org/index.php/rsd/article/view/29387
10.33448/rsd-v11i7.29387
url https://rsdjournal.org/index.php/rsd/article/view/29387
identifier_str_mv 10.33448/rsd-v11i7.29387
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv https://rsdjournal.org/index.php/rsd/article/view/29387/25970
dc.rights.driver.fl_str_mv https://creativecommons.org/licenses/by/4.0
info:eu-repo/semantics/openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by/4.0
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Research, Society and Development
publisher.none.fl_str_mv Research, Society and Development
dc.source.none.fl_str_mv Research, Society and Development; Vol. 11 No. 7; e38211729387
Research, Society and Development; Vol. 11 Núm. 7; e38211729387
Research, Society and Development; v. 11 n. 7; e38211729387
2525-3409
reponame:Research, Society and Development
instname:Universidade Federal de Itajubá (UNIFEI)
instacron:UNIFEI
instname_str Universidade Federal de Itajubá (UNIFEI)
instacron_str UNIFEI
institution UNIFEI
reponame_str Research, Society and Development
collection Research, Society and Development
repository.name.fl_str_mv Research, Society and Development - Universidade Federal de Itajubá (UNIFEI)
repository.mail.fl_str_mv rsd.articles@gmail.com
_version_ 1797052765872062464

Registros relacionados