Development stage at packaging affects viability of in vitro produced bovine embryos

Detalhes bibliográficos
Autor(a) principal: Silveira, Mariana da Mata
Data de Publicação: 2020
Outros Autores: Marques, Thaisa Campos, Silva, Marco Antônio Pereira da, Leão, Karen Martins
Tipo de documento: Artigo
Idioma: por
Título da fonte: Research, Society and Development
Texto Completo: https://rsdjournal.org/index.php/rsd/article/view/3615
Resumo: The objective of this study was to evaluate the viability of in vitro produced embryos (IVPE) bovine at different developmental stages, maintained at 36ºC at different transport times. On the seventh day of in vitro culture, grade I and II embryos at early blastocyst, blastocyst, and expanded blastocyst stages were selected, packaged and randomly distributed to three transport times in a portable incubator at 36°C: 6, 9 and 12 hours. After the treatment time, embryos were re-cultured in culture medium for 72 hours and evaluated for quality and development. Hatching and degeneration rate data were analyzed by Chi-square test. Embryos packaged at the expanded blastocyst stage showed higher hatching rates and lower degeneration rates. Embryos packaged at the expanded blastocyst stage showed higher viability compared to those at early blastocyst, regardless of transport time, and blastocysts transported for 6 and 9 hours.
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spelling Development stage at packaging affects viability of in vitro produced bovine embryosEtapa de desarrollo en llenado afecta la viabilidad de los embriones bovinos producidos in vitroEstágio de desenvolvimento no envase afeta a viabilidade de embriões bovinos produzidos in vitroDesarrollo embrionarioTransporte embrionarioFertilización in vitroOPU-FIV.Desenvolvimento embrionárioTransporte embrionárioFertilização in vitroOPU-FIV.Embryonic developmentEmbryonic transportin vitro fertilizationOPU-IVF.The objective of this study was to evaluate the viability of in vitro produced embryos (IVPE) bovine at different developmental stages, maintained at 36ºC at different transport times. On the seventh day of in vitro culture, grade I and II embryos at early blastocyst, blastocyst, and expanded blastocyst stages were selected, packaged and randomly distributed to three transport times in a portable incubator at 36°C: 6, 9 and 12 hours. After the treatment time, embryos were re-cultured in culture medium for 72 hours and evaluated for quality and development. Hatching and degeneration rate data were analyzed by Chi-square test. Embryos packaged at the expanded blastocyst stage showed higher hatching rates and lower degeneration rates. Embryos packaged at the expanded blastocyst stage showed higher viability compared to those at early blastocyst, regardless of transport time, and blastocysts transported for 6 and 9 hours.El objetivo de este estudio fue evaluar la viabilidad de embriones bovinos producidos in vitro (IVEP) en diferentes etapas de desarrollo, mantenidos a 36ºC en diferentes tiempos de transporte. En el séptimo día de cultivo in vitro, los embriones de grado I y II en las primeras etapas del blastocisto, blastocisto y blastocisto expandido fueron seleccionados, empaquetados y distribuidos aleatoriamente durante tres tiempos de transporte en una incubadora portátil a    36°C: 6, 9 y 12 horas. Después del tiempo de tratamiento, los embriones se volvieron a cultivar en medio de cultivo durante 72 horas y se evaluó su calidad y desarrollo. La tasa de eclosión y los datos de degeneración se analizaron mediante la prueba de chi-cuadrado. Los embriones empaquetados en la etapa de blastocisto expandido mostraron tasas de eclosión más altas y tasas de degeneración más bajas. Los embriones empaquetados en la etapa de blastocisto expandido mostraron una mayor viabilidad en comparación con los del blastocisto inicial, independientemente del tiempo de transporte, y los blastocistos transportados durante 6 y 9 horas.O objetivo deste estudo foi avaliar a viabilidade de embriões produzidos in vitro (IVPE) bovinos em diferentes estágios de desenvolvimento, mantidos a 36ºC em diferentes tempos de transporte. No sétimo dia de cultura in vitro, os embriões grau I e II nos estágios iniciais do blastocisto, blastocisto e blastocisto expandido foram selecionados, envasados e distribuídos aleatoriamente por três tempos de transporte em uma incubadora portátil a 36°C: 6, 9 e 12 horas. Após o tempo de tratamento, os embriões foram re-cultivados em meio de cultivo por 72 horas e avaliados quanto à qualidade e desenvolvimento. Os dados de taxa de eclosão e degeneração foram analisados pelo teste do qui-quadrado. Os embriões envasados no estágio de blastocisto expandido apresentaram maiores taxas de eclosão e menores taxas de degeneração. Os embriões envasados no estágio de blastocisto expandido mostraram maior viabilidade em comparação com os do blastocisto inicial, independentemente do tempo de transporte, e os blastocistos transportados por 6 e 9 horas.Research, Society and Development2020-04-21info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://rsdjournal.org/index.php/rsd/article/view/361510.33448/rsd-v9i6.3615Research, Society and Development; Vol. 9 No. 6; e134963615Research, Society and Development; Vol. 9 Núm. 6; e134963615Research, Society and Development; v. 9 n. 6; e1349636152525-3409reponame:Research, Society and Developmentinstname:Universidade Federal de Itajubá (UNIFEI)instacron:UNIFEIporhttps://rsdjournal.org/index.php/rsd/article/view/3615/3859Copyright (c) 2020 Mariana da Mata Silveira, Karen Martins Leão, Marco Antônio Pereira da Silva, THAISA CAMPOS MARQUESinfo:eu-repo/semantics/openAccessSilveira, Mariana da MataMarques, Thaisa CamposSilva, Marco Antônio Pereira daLeão, Karen Martins2020-08-20T18:05:46Zoai:ojs.pkp.sfu.ca:article/3615Revistahttps://rsdjournal.org/index.php/rsd/indexPUBhttps://rsdjournal.org/index.php/rsd/oairsd.articles@gmail.com2525-34092525-3409opendoar:2024-01-17T09:27:45.465431Research, Society and Development - Universidade Federal de Itajubá (UNIFEI)false
dc.title.none.fl_str_mv Development stage at packaging affects viability of in vitro produced bovine embryos
Etapa de desarrollo en llenado afecta la viabilidad de los embriones bovinos producidos in vitro
Estágio de desenvolvimento no envase afeta a viabilidade de embriões bovinos produzidos in vitro
title Development stage at packaging affects viability of in vitro produced bovine embryos
spellingShingle Development stage at packaging affects viability of in vitro produced bovine embryos
Silveira, Mariana da Mata
Desarrollo embrionario
Transporte embrionario
Fertilización in vitro
OPU-FIV.
Desenvolvimento embrionário
Transporte embrionário
Fertilização in vitro
OPU-FIV.
Embryonic development
Embryonic transport
in vitro fertilization
OPU-IVF.
title_short Development stage at packaging affects viability of in vitro produced bovine embryos
title_full Development stage at packaging affects viability of in vitro produced bovine embryos
title_fullStr Development stage at packaging affects viability of in vitro produced bovine embryos
title_full_unstemmed Development stage at packaging affects viability of in vitro produced bovine embryos
title_sort Development stage at packaging affects viability of in vitro produced bovine embryos
author Silveira, Mariana da Mata
author_facet Silveira, Mariana da Mata
Marques, Thaisa Campos
Silva, Marco Antônio Pereira da
Leão, Karen Martins
author_role author
author2 Marques, Thaisa Campos
Silva, Marco Antônio Pereira da
Leão, Karen Martins
author2_role author
author
author
dc.contributor.author.fl_str_mv Silveira, Mariana da Mata
Marques, Thaisa Campos
Silva, Marco Antônio Pereira da
Leão, Karen Martins
dc.subject.por.fl_str_mv Desarrollo embrionario
Transporte embrionario
Fertilización in vitro
OPU-FIV.
Desenvolvimento embrionário
Transporte embrionário
Fertilização in vitro
OPU-FIV.
Embryonic development
Embryonic transport
in vitro fertilization
OPU-IVF.
topic Desarrollo embrionario
Transporte embrionario
Fertilización in vitro
OPU-FIV.
Desenvolvimento embrionário
Transporte embrionário
Fertilização in vitro
OPU-FIV.
Embryonic development
Embryonic transport
in vitro fertilization
OPU-IVF.
description The objective of this study was to evaluate the viability of in vitro produced embryos (IVPE) bovine at different developmental stages, maintained at 36ºC at different transport times. On the seventh day of in vitro culture, grade I and II embryos at early blastocyst, blastocyst, and expanded blastocyst stages were selected, packaged and randomly distributed to three transport times in a portable incubator at 36°C: 6, 9 and 12 hours. After the treatment time, embryos were re-cultured in culture medium for 72 hours and evaluated for quality and development. Hatching and degeneration rate data were analyzed by Chi-square test. Embryos packaged at the expanded blastocyst stage showed higher hatching rates and lower degeneration rates. Embryos packaged at the expanded blastocyst stage showed higher viability compared to those at early blastocyst, regardless of transport time, and blastocysts transported for 6 and 9 hours.
publishDate 2020
dc.date.none.fl_str_mv 2020-04-21
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv https://rsdjournal.org/index.php/rsd/article/view/3615
10.33448/rsd-v9i6.3615
url https://rsdjournal.org/index.php/rsd/article/view/3615
identifier_str_mv 10.33448/rsd-v9i6.3615
dc.language.iso.fl_str_mv por
language por
dc.relation.none.fl_str_mv https://rsdjournal.org/index.php/rsd/article/view/3615/3859
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Research, Society and Development
publisher.none.fl_str_mv Research, Society and Development
dc.source.none.fl_str_mv Research, Society and Development; Vol. 9 No. 6; e134963615
Research, Society and Development; Vol. 9 Núm. 6; e134963615
Research, Society and Development; v. 9 n. 6; e134963615
2525-3409
reponame:Research, Society and Development
instname:Universidade Federal de Itajubá (UNIFEI)
instacron:UNIFEI
instname_str Universidade Federal de Itajubá (UNIFEI)
instacron_str UNIFEI
institution UNIFEI
reponame_str Research, Society and Development
collection Research, Society and Development
repository.name.fl_str_mv Research, Society and Development - Universidade Federal de Itajubá (UNIFEI)
repository.mail.fl_str_mv rsd.articles@gmail.com
_version_ 1797052802408644608

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