Composição química, atividade antimicrobiana, inseticida e antioxidante do óleo essencial e extratos de Guarea kunthiana A. Juss

Detalhes bibliográficos
Autor(a) principal: Pandini, Jessica Angela
Data de Publicação: 2014
Tipo de documento: Dissertação
Idioma: por
Título da fonte: Biblioteca Digital de Teses e Dissertações do UNIOESTE
Texto Completo: http://tede.unioeste.br:8080/tede/handle/tede/693
Resumo: The present study aimed to determine the chemical composition of the essential oil of Guarea kunthiana A Juss by the method of gas chromatography coupled to mass spectrometry (GC-MS), evaluate the antimicrobial activity of this oil against different Gram positive and Gram negative bacteria, and yeast: Escherichia coli, Salmonella entérica subsp. entérica, Pseudomonas aeruginosa, Proteus mirabilis, Klebsiella pneumoniae, Staphylococcus aureus, Enterococcus faecalis, Staphylococcus epidermidis, Bacillus subtillis e Candida albicans. Evaluate the antimicrobial activity of essential oil and aqueous extracts, ethyl acetate and alcohol against different serotypes of Salmonella in poultry origin: Enteritidis, Infantis, Typhimurium, Heildelberg, Mbandaka, Give, Saintpaul, Orion, Gallinarum e Agona. Determine the insecticidal activity of the oil and extracts against larvae and adults of Alphitobius diaperinus (Panzer 1797) (Coleoptera: Tenebrionidae), and finally evaluate the antioxidant activity of essential oil and extracts by the capture of the hydrazyl free radical 2,2-diphenyl-1-picryl (DPPH). The analyses of GC-MS resulted in the identification of 13 constituents, representing 96.52% of the total area on the essential oil. The main compounds identified were α-Zingiberene (34.48%), β-Sesquifelandreno (22.90%) and α-Curcumene (16.17%). Regarding antimicrobial activity of the essential oil was effective against all micro-organisms tested except for the bacteria E.coli e K. pneumoniae, which presented themselves resistant to the action of the oil. For Salmonella serotypes evaluated, the essential oil had greater activity against serotypes Infantis, Typhimurium e Give, with values of MIC and MBC of 54,6 μg/mL for both, for the other serotypes tested the action of the oil was classified as moderate, weak and inactive. Regarding extracts all serotypes tested were susceptible to the extract of ethyl acetate, followed by alcoholic extract, the aqueous extract showed no antimicrobial activity. The results concerning the insecticidal activity of the essential oil revealed that it provided better activity against larvae at a concentration of 200 mg / mL (28%) compared to adults the mortality rate was low (12%) at this concentration. With the extracts, the best results were found for the alcoholic extract at a concentration of 10%, with values of 34 and 44% respectively, adults and larvae. The values of antioxidant activity showed no significant differences between the synthetic antioxidant butylated hydroxytoluene (BHT), essential oil and alcoholic extract, revealing that both the essential oil as alcoholic extract of G. kunthiana demonstrated high antioxidant capacity
id UNIOESTE-1_cfb1a3807273b830254391eab2113436
oai_identifier_str oai:tede.unioeste.br:tede/693
network_acronym_str UNIOESTE-1
network_name_str Biblioteca Digital de Teses e Dissertações do UNIOESTE
repository_id_str
spelling Pinto, Fabiana Gisele da SilvaCPF:19148289841http://lattes.cnpq.br/9361463429150328CPF:06906799983http://lattes.cnpq.br/8960326446119601Pandini, Jessica Angela2017-07-10T14:38:22Z2014-09-192014-06-06PANDINI, Jessica Angela. Chemical composition, antimicrobial, insecticidal and antioxidant properties of the essential oil and extracts of Guarea kunthiana A. Juss. 2014. 89 f. Dissertação (Mestrado em Conservação e Manejo de Recursos Naturais) - Universidade Estadual do Oeste do Parana, Cascavel, 2014.http://tede.unioeste.br:8080/tede/handle/tede/693The present study aimed to determine the chemical composition of the essential oil of Guarea kunthiana A Juss by the method of gas chromatography coupled to mass spectrometry (GC-MS), evaluate the antimicrobial activity of this oil against different Gram positive and Gram negative bacteria, and yeast: Escherichia coli, Salmonella entérica subsp. entérica, Pseudomonas aeruginosa, Proteus mirabilis, Klebsiella pneumoniae, Staphylococcus aureus, Enterococcus faecalis, Staphylococcus epidermidis, Bacillus subtillis e Candida albicans. Evaluate the antimicrobial activity of essential oil and aqueous extracts, ethyl acetate and alcohol against different serotypes of Salmonella in poultry origin: Enteritidis, Infantis, Typhimurium, Heildelberg, Mbandaka, Give, Saintpaul, Orion, Gallinarum e Agona. Determine the insecticidal activity of the oil and extracts against larvae and adults of Alphitobius diaperinus (Panzer 1797) (Coleoptera: Tenebrionidae), and finally evaluate the antioxidant activity of essential oil and extracts by the capture of the hydrazyl free radical 2,2-diphenyl-1-picryl (DPPH). The analyses of GC-MS resulted in the identification of 13 constituents, representing 96.52% of the total area on the essential oil. The main compounds identified were α-Zingiberene (34.48%), β-Sesquifelandreno (22.90%) and α-Curcumene (16.17%). Regarding antimicrobial activity of the essential oil was effective against all micro-organisms tested except for the bacteria E.coli e K. pneumoniae, which presented themselves resistant to the action of the oil. For Salmonella serotypes evaluated, the essential oil had greater activity against serotypes Infantis, Typhimurium e Give, with values of MIC and MBC of 54,6 μg/mL for both, for the other serotypes tested the action of the oil was classified as moderate, weak and inactive. Regarding extracts all serotypes tested were susceptible to the extract of ethyl acetate, followed by alcoholic extract, the aqueous extract showed no antimicrobial activity. The results concerning the insecticidal activity of the essential oil revealed that it provided better activity against larvae at a concentration of 200 mg / mL (28%) compared to adults the mortality rate was low (12%) at this concentration. With the extracts, the best results were found for the alcoholic extract at a concentration of 10%, with values of 34 and 44% respectively, adults and larvae. The values of antioxidant activity showed no significant differences between the synthetic antioxidant butylated hydroxytoluene (BHT), essential oil and alcoholic extract, revealing that both the essential oil as alcoholic extract of G. kunthiana demonstrated high antioxidant capacityO presente estudo teve por objetivo determinar a composição química do óleo essencial de Guarea kunthiana A Juss pelo método de cromatografia gasosa acoplada à espectrometria de massas (CG-EM), avaliar a atividade antimicrobiana deste óleo frente a diferentes bactérias Gram-positivas e Gram- negativas e uma levedura: Escherichia coli, Salmonella entérica subsp. entérica, Pseudomonas aeruginosa, Proteus mirabilis, Klebsiella pneumoniae, Staphylococcus aureus, Enterococcus faecalis, Staphylococcus epidermidis, Bacillus subtillis e Candida albicans. Avaliar a atividade antimicrobiana do óleo essencial e dos extratos aquoso, acetato de etila e alcoólico frente a diferentes sorotipos de Salmonella de origem avícola: Enteritidis, Infantis, Typhimurium, Heildelberg, Mbandaka, Give, Saintpaul, Orion, Gallinarum e Agona. Determinar a atividade inseticida do óleo e dos extratos frente a larvas e adultos de Alphitobius diaperinus (Panzer 1797) (Coleoptera: Tenebrionidae), e por último avaliar a atividade antioxidante do óleo essencial e dos extratos pelo método de captura do radical livre 2,2-difenil-1-picril hidrazil (DPPH). As análises de CG-EM resultaram na identificação de 13 constituintes, representando 96,52% da área relativa total do óleo essencial. Os principais compostos identificados foram α-Zingibereno (34,48%), β-Sesquifelandreno (22,90%) e α-Curcumeno (16,17%). Em relação à atividade antimicrobiana o óleo essencial foi efetivo frente a todos os micro-organismos testados exceto para as bactérias E.coli e K. pneumoniae, as quais se apresentaram resistentes à ação do óleo. Para os sorotipos de Salmonella avaliados, o óleo essencial exerceu maior atividade frente aos sorotipos Infantis, Typhimurium e Give, com valores de CIM e CBM de 54,6 μg/mL para ambos, para os demais sorotipos testados a ação do óleo foi classificada como moderada, fraca e inativa. Em relação aos extratos todos os sorotipos testados foram suscetíveis ao extrato de acetato de etila, seguido do extrato alcoólico, o extrato aquoso não apresentou atividade antimicrobiana. Os resultados referentes à atividade inseticida do óleo essencial revelaram que este exerceu melhor atividade frente às larvas na concentração de 200 mg/mL (28%), em relação aos adultos a porcentagem de mortalidade foi baixa (12%) nesta concentração. Com os extratos, os melhores resultados foram encontrados para o extrato alcoólico na concentração de 10%, com valores de 34 e 44% para larvas e adultos respectivamente. Os valores de atividade antioxidante revelaram que não houve diferenças significativas entre o antioxidante sintético butil-hidroxi-tolueno (BHT), o óleo essencial e o extrato alcoólico, revelando que tanto o óleo essencial quanto extrato alcoólico de G. kunthiana demonstraram elevada capacidade antioxidanteMade available in DSpace on 2017-07-10T14:38:22Z (GMT). No. of bitstreams: 1 Jessica Pandini.pdf: 653531 bytes, checksum: 10e2067d39b473edba9024e91143df5b (MD5) Previous issue date: 2014-06-06Coordenação de Aperfeiçoamento de Pessoal de Nível Superiorapplication/pdfporUniversidade Estadual do Oeste do ParanaPrograma de Pós-Graduação Stricto Sensu em Conservação e Manejo de Recursos NaturaisUNIOESTEBRConservação e Manejo de Recursos NaturaisAção antimicrobianaGuarea kunthianaextratos vegetaisóleo essencial Salmonella sppcascudinho dos aviáriosAntimicrobial actionGuarea kunthianaplant extractsessential oil Salmonella sppDarkiling beetlesCNPQ::CIENCIAS BIOLOGICAS::BIOLOGIA GERALComposição química, atividade antimicrobiana, inseticida e antioxidante do óleo essencial e extratos de Guarea kunthiana A. JussChemical composition, antimicrobial, insecticidal and antioxidant properties of the essential oil and extracts of Guarea kunthiana A. Jussinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/masterThesisinfo:eu-repo/semantics/openAccessreponame:Biblioteca Digital de Teses e Dissertações do UNIOESTEinstname:Universidade Estadual do Oeste do Paraná (UNIOESTE)instacron:UNIOESTEORIGINALJessica Pandini.pdfapplication/pdf653531http://tede.unioeste.br:8080/tede/bitstream/tede/693/1/Jessica+Pandini.pdf10e2067d39b473edba9024e91143df5bMD51tede/6932017-07-10 11:38:22.935oai:tede.unioeste.br:tede/693Biblioteca Digital de Teses e Dissertaçõeshttp://tede.unioeste.br/PUBhttp://tede.unioeste.br/oai/requestbiblioteca.repositorio@unioeste.bropendoar:2017-07-10T14:38:22Biblioteca Digital de Teses e Dissertações do UNIOESTE - Universidade Estadual do Oeste do Paraná (UNIOESTE)false
dc.title.por.fl_str_mv Composição química, atividade antimicrobiana, inseticida e antioxidante do óleo essencial e extratos de Guarea kunthiana A. Juss
dc.title.alternative.eng.fl_str_mv Chemical composition, antimicrobial, insecticidal and antioxidant properties of the essential oil and extracts of Guarea kunthiana A. Juss
title Composição química, atividade antimicrobiana, inseticida e antioxidante do óleo essencial e extratos de Guarea kunthiana A. Juss
spellingShingle Composição química, atividade antimicrobiana, inseticida e antioxidante do óleo essencial e extratos de Guarea kunthiana A. Juss
Pandini, Jessica Angela
Ação antimicrobiana
Guarea kunthiana
extratos vegetais
óleo essencial Salmonella spp
cascudinho dos aviários
Antimicrobial action
Guarea kunthiana
plant extracts
essential oil Salmonella spp
Darkiling beetles
CNPQ::CIENCIAS BIOLOGICAS::BIOLOGIA GERAL
title_short Composição química, atividade antimicrobiana, inseticida e antioxidante do óleo essencial e extratos de Guarea kunthiana A. Juss
title_full Composição química, atividade antimicrobiana, inseticida e antioxidante do óleo essencial e extratos de Guarea kunthiana A. Juss
title_fullStr Composição química, atividade antimicrobiana, inseticida e antioxidante do óleo essencial e extratos de Guarea kunthiana A. Juss
title_full_unstemmed Composição química, atividade antimicrobiana, inseticida e antioxidante do óleo essencial e extratos de Guarea kunthiana A. Juss
title_sort Composição química, atividade antimicrobiana, inseticida e antioxidante do óleo essencial e extratos de Guarea kunthiana A. Juss
author Pandini, Jessica Angela
author_facet Pandini, Jessica Angela
author_role author
dc.contributor.advisor1.fl_str_mv Pinto, Fabiana Gisele da Silva
dc.contributor.advisor1ID.fl_str_mv CPF:19148289841
dc.contributor.advisor1Lattes.fl_str_mv http://lattes.cnpq.br/9361463429150328
dc.contributor.authorID.fl_str_mv CPF:06906799983
dc.contributor.authorLattes.fl_str_mv http://lattes.cnpq.br/8960326446119601
dc.contributor.author.fl_str_mv Pandini, Jessica Angela
contributor_str_mv Pinto, Fabiana Gisele da Silva
dc.subject.por.fl_str_mv Ação antimicrobiana
Guarea kunthiana
extratos vegetais
óleo essencial Salmonella spp
cascudinho dos aviários
topic Ação antimicrobiana
Guarea kunthiana
extratos vegetais
óleo essencial Salmonella spp
cascudinho dos aviários
Antimicrobial action
Guarea kunthiana
plant extracts
essential oil Salmonella spp
Darkiling beetles
CNPQ::CIENCIAS BIOLOGICAS::BIOLOGIA GERAL
dc.subject.eng.fl_str_mv Antimicrobial action
Guarea kunthiana
plant extracts
essential oil Salmonella spp
Darkiling beetles
dc.subject.cnpq.fl_str_mv CNPQ::CIENCIAS BIOLOGICAS::BIOLOGIA GERAL
description The present study aimed to determine the chemical composition of the essential oil of Guarea kunthiana A Juss by the method of gas chromatography coupled to mass spectrometry (GC-MS), evaluate the antimicrobial activity of this oil against different Gram positive and Gram negative bacteria, and yeast: Escherichia coli, Salmonella entérica subsp. entérica, Pseudomonas aeruginosa, Proteus mirabilis, Klebsiella pneumoniae, Staphylococcus aureus, Enterococcus faecalis, Staphylococcus epidermidis, Bacillus subtillis e Candida albicans. Evaluate the antimicrobial activity of essential oil and aqueous extracts, ethyl acetate and alcohol against different serotypes of Salmonella in poultry origin: Enteritidis, Infantis, Typhimurium, Heildelberg, Mbandaka, Give, Saintpaul, Orion, Gallinarum e Agona. Determine the insecticidal activity of the oil and extracts against larvae and adults of Alphitobius diaperinus (Panzer 1797) (Coleoptera: Tenebrionidae), and finally evaluate the antioxidant activity of essential oil and extracts by the capture of the hydrazyl free radical 2,2-diphenyl-1-picryl (DPPH). The analyses of GC-MS resulted in the identification of 13 constituents, representing 96.52% of the total area on the essential oil. The main compounds identified were α-Zingiberene (34.48%), β-Sesquifelandreno (22.90%) and α-Curcumene (16.17%). Regarding antimicrobial activity of the essential oil was effective against all micro-organisms tested except for the bacteria E.coli e K. pneumoniae, which presented themselves resistant to the action of the oil. For Salmonella serotypes evaluated, the essential oil had greater activity against serotypes Infantis, Typhimurium e Give, with values of MIC and MBC of 54,6 μg/mL for both, for the other serotypes tested the action of the oil was classified as moderate, weak and inactive. Regarding extracts all serotypes tested were susceptible to the extract of ethyl acetate, followed by alcoholic extract, the aqueous extract showed no antimicrobial activity. The results concerning the insecticidal activity of the essential oil revealed that it provided better activity against larvae at a concentration of 200 mg / mL (28%) compared to adults the mortality rate was low (12%) at this concentration. With the extracts, the best results were found for the alcoholic extract at a concentration of 10%, with values of 34 and 44% respectively, adults and larvae. The values of antioxidant activity showed no significant differences between the synthetic antioxidant butylated hydroxytoluene (BHT), essential oil and alcoholic extract, revealing that both the essential oil as alcoholic extract of G. kunthiana demonstrated high antioxidant capacity
publishDate 2014
dc.date.available.fl_str_mv 2014-09-19
dc.date.issued.fl_str_mv 2014-06-06
dc.date.accessioned.fl_str_mv 2017-07-10T14:38:22Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/masterThesis
format masterThesis
status_str publishedVersion
dc.identifier.citation.fl_str_mv PANDINI, Jessica Angela. Chemical composition, antimicrobial, insecticidal and antioxidant properties of the essential oil and extracts of Guarea kunthiana A. Juss. 2014. 89 f. Dissertação (Mestrado em Conservação e Manejo de Recursos Naturais) - Universidade Estadual do Oeste do Parana, Cascavel, 2014.
dc.identifier.uri.fl_str_mv http://tede.unioeste.br:8080/tede/handle/tede/693
identifier_str_mv PANDINI, Jessica Angela. Chemical composition, antimicrobial, insecticidal and antioxidant properties of the essential oil and extracts of Guarea kunthiana A. Juss. 2014. 89 f. Dissertação (Mestrado em Conservação e Manejo de Recursos Naturais) - Universidade Estadual do Oeste do Parana, Cascavel, 2014.
url http://tede.unioeste.br:8080/tede/handle/tede/693
dc.language.iso.fl_str_mv por
language por
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Universidade Estadual do Oeste do Parana
dc.publisher.program.fl_str_mv Programa de Pós-Graduação Stricto Sensu em Conservação e Manejo de Recursos Naturais
dc.publisher.initials.fl_str_mv UNIOESTE
dc.publisher.country.fl_str_mv BR
dc.publisher.department.fl_str_mv Conservação e Manejo de Recursos Naturais
publisher.none.fl_str_mv Universidade Estadual do Oeste do Parana
dc.source.none.fl_str_mv reponame:Biblioteca Digital de Teses e Dissertações do UNIOESTE
instname:Universidade Estadual do Oeste do Paraná (UNIOESTE)
instacron:UNIOESTE
instname_str Universidade Estadual do Oeste do Paraná (UNIOESTE)
instacron_str UNIOESTE
institution UNIOESTE
reponame_str Biblioteca Digital de Teses e Dissertações do UNIOESTE
collection Biblioteca Digital de Teses e Dissertações do UNIOESTE
bitstream.url.fl_str_mv http://tede.unioeste.br:8080/tede/bitstream/tede/693/1/Jessica+Pandini.pdf
bitstream.checksum.fl_str_mv 10e2067d39b473edba9024e91143df5b
bitstream.checksumAlgorithm.fl_str_mv MD5
repository.name.fl_str_mv Biblioteca Digital de Teses e Dissertações do UNIOESTE - Universidade Estadual do Oeste do Paraná (UNIOESTE)
repository.mail.fl_str_mv biblioteca.repositorio@unioeste.br
_version_ 1801124517177720832