IN VITRO ASSESSMENT OF CYTOTOXICITY AND OXIDATIVE STRESS PARAMETERS OF NATURAL RUBBER LATEX MEMBRANES STABILIZED WITH DIFFERENT CONCENTRATIONS OF AMMONIA IN CHO-K1 CELLS

Detalhes bibliográficos
Autor(a) principal: Araujo, Caroline de Souza
Data de Publicação: 2020
Outros Autores: Cavalcante, Dalita Gomes Silva Morais, Braga, Nathália Oliveira, Gomes, Andressa Silva, Yoshihara, Eidi, Kerche, Leandra Ernst, Job, Aldo Eloizo
Tipo de documento: Artigo
Idioma: por
Título da fonte: Colloquium Vitae
Texto Completo: http://revistas.unoeste.br/index.php/cv/article/view/2446
Resumo: The purpose of this study was to assess in vitro cytotoxicity of natural rubber (NR) latex membranes stabilized with different concentrations of ammonia, as well as evaluate possible changes in oxidative stress parameters. To this end, CHO-k1 cells were exposed to NR membranes for 24 h. Cytotoxicity was assessed by the MTT assay and the oxidative stress parameters were established by glutathione (GSH) and malondialdehyde (MDA) levels. The results show that the ammonia present in the NR latex membranes can deplete cell viability and enhance GSH consumption in the cells. NR latex membranes stabilized with 2.00% of ammonia were cytotoxic to the cell lineage used in this study. From these results, it is possible to infer that the use of ammonia to stabilize NR latex membranes with biomedical purposes has to be done carefully, to avoid possible harmful effects to human health.
id UNIOESTE-2_1f472fe2889f9f2863fe1bd0a130219d
oai_identifier_str oai:ojs.pkp.sfu.ca:article/2446
network_acronym_str UNIOESTE-2
network_name_str Colloquium Vitae
repository_id_str
spelling IN VITRO ASSESSMENT OF CYTOTOXICITY AND OXIDATIVE STRESS PARAMETERS OF NATURAL RUBBER LATEX MEMBRANES STABILIZED WITH DIFFERENT CONCENTRATIONS OF AMMONIA IN CHO-K1 CELLSNatural rubber latexmembranesammoniacytotoxicityGSH.The purpose of this study was to assess in vitro cytotoxicity of natural rubber (NR) latex membranes stabilized with different concentrations of ammonia, as well as evaluate possible changes in oxidative stress parameters. To this end, CHO-k1 cells were exposed to NR membranes for 24 h. Cytotoxicity was assessed by the MTT assay and the oxidative stress parameters were established by glutathione (GSH) and malondialdehyde (MDA) levels. The results show that the ammonia present in the NR latex membranes can deplete cell viability and enhance GSH consumption in the cells. NR latex membranes stabilized with 2.00% of ammonia were cytotoxic to the cell lineage used in this study. From these results, it is possible to infer that the use of ammonia to stabilize NR latex membranes with biomedical purposes has to be done carefully, to avoid possible harmful effects to human health.Universidade do Oeste Paulista - UNOESTE2020-01-17info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionAvaliado por paresapplication/pdfhttp://revistas.unoeste.br/index.php/cv/article/view/2446Colloquium Vitae. ISSN: 1984-6436; v. 11 n. 3 (2019): Colloquium Vitae, Vol. 11, n. 3; 32-401984-6436reponame:Colloquium Vitaeinstname:Universidade do Oeste Paulista (UNOESTE)instacron:UNIOESTEporhttp://revistas.unoeste.br/index.php/cv/article/view/2446/2910Copyright (c) 2020 Colloquium Vitae. ISSN: 1984-6436info:eu-repo/semantics/openAccessAraujo, Caroline de SouzaCavalcante, Dalita Gomes Silva MoraisBraga, Nathália OliveiraGomes, Andressa SilvaYoshihara, EidiKerche, Leandra ErnstJob, Aldo Eloizo2020-07-03T17:49:27Zoai:ojs.pkp.sfu.ca:article/2446Revistahttps://revistas.unoeste.br/index.php/cv/indexONGhttp://revistas.unoeste.br/revistas/ojs/index.php/cv/oaijgjunior@unoeste.br||jgjunior@unoeste.br1984-64361984-6436opendoar:2020-07-03T17:49:27Colloquium Vitae - Universidade do Oeste Paulista (UNOESTE)false
dc.title.none.fl_str_mv IN VITRO ASSESSMENT OF CYTOTOXICITY AND OXIDATIVE STRESS PARAMETERS OF NATURAL RUBBER LATEX MEMBRANES STABILIZED WITH DIFFERENT CONCENTRATIONS OF AMMONIA IN CHO-K1 CELLS
title IN VITRO ASSESSMENT OF CYTOTOXICITY AND OXIDATIVE STRESS PARAMETERS OF NATURAL RUBBER LATEX MEMBRANES STABILIZED WITH DIFFERENT CONCENTRATIONS OF AMMONIA IN CHO-K1 CELLS
spellingShingle IN VITRO ASSESSMENT OF CYTOTOXICITY AND OXIDATIVE STRESS PARAMETERS OF NATURAL RUBBER LATEX MEMBRANES STABILIZED WITH DIFFERENT CONCENTRATIONS OF AMMONIA IN CHO-K1 CELLS
Araujo, Caroline de Souza
Natural rubber latex
membranes
ammonia
cytotoxicity
GSH.
title_short IN VITRO ASSESSMENT OF CYTOTOXICITY AND OXIDATIVE STRESS PARAMETERS OF NATURAL RUBBER LATEX MEMBRANES STABILIZED WITH DIFFERENT CONCENTRATIONS OF AMMONIA IN CHO-K1 CELLS
title_full IN VITRO ASSESSMENT OF CYTOTOXICITY AND OXIDATIVE STRESS PARAMETERS OF NATURAL RUBBER LATEX MEMBRANES STABILIZED WITH DIFFERENT CONCENTRATIONS OF AMMONIA IN CHO-K1 CELLS
title_fullStr IN VITRO ASSESSMENT OF CYTOTOXICITY AND OXIDATIVE STRESS PARAMETERS OF NATURAL RUBBER LATEX MEMBRANES STABILIZED WITH DIFFERENT CONCENTRATIONS OF AMMONIA IN CHO-K1 CELLS
title_full_unstemmed IN VITRO ASSESSMENT OF CYTOTOXICITY AND OXIDATIVE STRESS PARAMETERS OF NATURAL RUBBER LATEX MEMBRANES STABILIZED WITH DIFFERENT CONCENTRATIONS OF AMMONIA IN CHO-K1 CELLS
title_sort IN VITRO ASSESSMENT OF CYTOTOXICITY AND OXIDATIVE STRESS PARAMETERS OF NATURAL RUBBER LATEX MEMBRANES STABILIZED WITH DIFFERENT CONCENTRATIONS OF AMMONIA IN CHO-K1 CELLS
author Araujo, Caroline de Souza
author_facet Araujo, Caroline de Souza
Cavalcante, Dalita Gomes Silva Morais
Braga, Nathália Oliveira
Gomes, Andressa Silva
Yoshihara, Eidi
Kerche, Leandra Ernst
Job, Aldo Eloizo
author_role author
author2 Cavalcante, Dalita Gomes Silva Morais
Braga, Nathália Oliveira
Gomes, Andressa Silva
Yoshihara, Eidi
Kerche, Leandra Ernst
Job, Aldo Eloizo
author2_role author
author
author
author
author
author
dc.contributor.author.fl_str_mv Araujo, Caroline de Souza
Cavalcante, Dalita Gomes Silva Morais
Braga, Nathália Oliveira
Gomes, Andressa Silva
Yoshihara, Eidi
Kerche, Leandra Ernst
Job, Aldo Eloizo
dc.subject.por.fl_str_mv Natural rubber latex
membranes
ammonia
cytotoxicity
GSH.
topic Natural rubber latex
membranes
ammonia
cytotoxicity
GSH.
description The purpose of this study was to assess in vitro cytotoxicity of natural rubber (NR) latex membranes stabilized with different concentrations of ammonia, as well as evaluate possible changes in oxidative stress parameters. To this end, CHO-k1 cells were exposed to NR membranes for 24 h. Cytotoxicity was assessed by the MTT assay and the oxidative stress parameters were established by glutathione (GSH) and malondialdehyde (MDA) levels. The results show that the ammonia present in the NR latex membranes can deplete cell viability and enhance GSH consumption in the cells. NR latex membranes stabilized with 2.00% of ammonia were cytotoxic to the cell lineage used in this study. From these results, it is possible to infer that the use of ammonia to stabilize NR latex membranes with biomedical purposes has to be done carefully, to avoid possible harmful effects to human health.
publishDate 2020
dc.date.none.fl_str_mv 2020-01-17
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Avaliado por pares
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://revistas.unoeste.br/index.php/cv/article/view/2446
url http://revistas.unoeste.br/index.php/cv/article/view/2446
dc.language.iso.fl_str_mv por
language por
dc.relation.none.fl_str_mv http://revistas.unoeste.br/index.php/cv/article/view/2446/2910
dc.rights.driver.fl_str_mv Copyright (c) 2020 Colloquium Vitae. ISSN: 1984-6436
info:eu-repo/semantics/openAccess
rights_invalid_str_mv Copyright (c) 2020 Colloquium Vitae. ISSN: 1984-6436
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Universidade do Oeste Paulista - UNOESTE
publisher.none.fl_str_mv Universidade do Oeste Paulista - UNOESTE
dc.source.none.fl_str_mv Colloquium Vitae. ISSN: 1984-6436; v. 11 n. 3 (2019): Colloquium Vitae, Vol. 11, n. 3; 32-40
1984-6436
reponame:Colloquium Vitae
instname:Universidade do Oeste Paulista (UNOESTE)
instacron:UNIOESTE
instname_str Universidade do Oeste Paulista (UNOESTE)
instacron_str UNIOESTE
institution UNIOESTE
reponame_str Colloquium Vitae
collection Colloquium Vitae
repository.name.fl_str_mv Colloquium Vitae - Universidade do Oeste Paulista (UNOESTE)
repository.mail.fl_str_mv jgjunior@unoeste.br||jgjunior@unoeste.br
_version_ 1800218925481328640

Registros relacionados