Schinus terebinthifolius (Brazilian peppertree) extract used as antifungal to control candida spp. in planktonic cultures and biofilms
Autor(a) principal: | |
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Data de Publicação: | 2020 |
Outros Autores: | , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.14295/bds.2020.v23i4.2077 http://hdl.handle.net/11449/205261 |
Resumo: | Objective: The use of medicinal plants may be an alternative method for the control of Candida spp. responsible for human infections. This study evaluated the antifungal effect of Schinus terebinthifolius extract (Brazilian Peppertree) on C. albicans, C. dubliniensis, C. glabrata, and C. krusei planktonic cultures and biofilms. Material and Methods: Minimum inhibitory concentration (MIC) and minimum fungal concentration (MFC) of the plant extract were determined by the broth microdilution method. Biofilms formed in microplate wells were exposed to the extract for 5 min (50, 100 and 200 mg/mL) or 24 h (25, 50 and 100 mg/mL). After determination of colony-forming units per milliliter (CFU/mL), the data were analyzed by one-way ANOVA and Tukey’s Test (P ≤ 0.05). Results: Different MIC (mg/mL) were found, such as 0.39 (C. dubliniensis), 1.56 (C. albicans), and 3.13 (C. glabrata and C. krusei). Besides, MFC (mg/mL) of 0.78 (C. dubliniensis) and 3.13 (C. albicans, C. glabrata and C. krusei) were also observed. Regarding the biofilms, significant reductions (log10) were found after 5 min and 24 h exposure to the plant extract, compared to the control group. However, C. dubliniensis was significantly affected only in 24 h treatment. Conclusion: S. terebinthifolius extract presented a significant antifungal effect on C. albicans, C. dubliniensis, C. glabrata, and C. Krusei both in planktonic cultures and biofilms. |
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Schinus terebinthifolius (Brazilian peppertree) extract used as antifungal to control candida spp. in planktonic cultures and biofilmsExtrato de schinus terebinthifolius (Pimenta rosa) utilizado como antifúngico para controlar candida spp. em culturas planctônicas e biofilmesBiofilmsBrazilian peppertreeCandidaPlanktonic culturesSchinus terebinthifoliusObjective: The use of medicinal plants may be an alternative method for the control of Candida spp. responsible for human infections. This study evaluated the antifungal effect of Schinus terebinthifolius extract (Brazilian Peppertree) on C. albicans, C. dubliniensis, C. glabrata, and C. krusei planktonic cultures and biofilms. Material and Methods: Minimum inhibitory concentration (MIC) and minimum fungal concentration (MFC) of the plant extract were determined by the broth microdilution method. Biofilms formed in microplate wells were exposed to the extract for 5 min (50, 100 and 200 mg/mL) or 24 h (25, 50 and 100 mg/mL). After determination of colony-forming units per milliliter (CFU/mL), the data were analyzed by one-way ANOVA and Tukey’s Test (P ≤ 0.05). Results: Different MIC (mg/mL) were found, such as 0.39 (C. dubliniensis), 1.56 (C. albicans), and 3.13 (C. glabrata and C. krusei). Besides, MFC (mg/mL) of 0.78 (C. dubliniensis) and 3.13 (C. albicans, C. glabrata and C. krusei) were also observed. Regarding the biofilms, significant reductions (log10) were found after 5 min and 24 h exposure to the plant extract, compared to the control group. However, C. dubliniensis was significantly affected only in 24 h treatment. Conclusion: S. terebinthifolius extract presented a significant antifungal effect on C. albicans, C. dubliniensis, C. glabrata, and C. Krusei both in planktonic cultures and biofilms.Federal University of Rio de Janeiro (UFRJ) Institute of Biodiversity and Sustainability (NUPEM)University of Taubaté (UNITAU) Basic Institute of BiosciencesSão Paulo State University (UNESP) Institute of Science and TechnologyUniversity Center Brazcubas Brazcubas Faculty of DentistryAnhembi Morumbi University School of MedicineSão Paulo State University (UNESP) Institute of Science and TechnologyUniversidade Federal do Rio de Janeiro (UFRJ)Basic Institute of BiosciencesUniversidade Estadual Paulista (Unesp)Brazcubas Faculty of DentistrySchool of MedicineViegas, Daiane de JesusAmêndola, IsabelaBotrel, Tássia Marchetti [UNESP]de OLIVEIRA, Felipe EduardoFigueira, Leandro Wagner [UNESP]Sper, Fábia Lugli [UNESP]de OLIVEIRA, Jonatas Rafaelde OLIVEIRA, Luciane Dias [UNESP]2021-06-25T10:12:27Z2021-06-25T10:12:27Z2020-01-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article1-7http://dx.doi.org/10.14295/bds.2020.v23i4.2077Brazilian Dental Science, v. 23, n. 4, p. 1-7, 2020.2178-6011http://hdl.handle.net/11449/20526110.14295/bds.2020.v23i4.20772-s2.0-85092080311Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengBrazilian Dental Scienceinfo:eu-repo/semantics/openAccess2021-10-23T12:24:10Zoai:repositorio.unesp.br:11449/205261Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462021-10-23T12:24:10Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Schinus terebinthifolius (Brazilian peppertree) extract used as antifungal to control candida spp. in planktonic cultures and biofilms Extrato de schinus terebinthifolius (Pimenta rosa) utilizado como antifúngico para controlar candida spp. em culturas planctônicas e biofilmes |
title |
Schinus terebinthifolius (Brazilian peppertree) extract used as antifungal to control candida spp. in planktonic cultures and biofilms |
spellingShingle |
Schinus terebinthifolius (Brazilian peppertree) extract used as antifungal to control candida spp. in planktonic cultures and biofilms Viegas, Daiane de Jesus Biofilms Brazilian peppertree Candida Planktonic cultures Schinus terebinthifolius |
title_short |
Schinus terebinthifolius (Brazilian peppertree) extract used as antifungal to control candida spp. in planktonic cultures and biofilms |
title_full |
Schinus terebinthifolius (Brazilian peppertree) extract used as antifungal to control candida spp. in planktonic cultures and biofilms |
title_fullStr |
Schinus terebinthifolius (Brazilian peppertree) extract used as antifungal to control candida spp. in planktonic cultures and biofilms |
title_full_unstemmed |
Schinus terebinthifolius (Brazilian peppertree) extract used as antifungal to control candida spp. in planktonic cultures and biofilms |
title_sort |
Schinus terebinthifolius (Brazilian peppertree) extract used as antifungal to control candida spp. in planktonic cultures and biofilms |
author |
Viegas, Daiane de Jesus |
author_facet |
Viegas, Daiane de Jesus Amêndola, Isabela Botrel, Tássia Marchetti [UNESP] de OLIVEIRA, Felipe Eduardo Figueira, Leandro Wagner [UNESP] Sper, Fábia Lugli [UNESP] de OLIVEIRA, Jonatas Rafael de OLIVEIRA, Luciane Dias [UNESP] |
author_role |
author |
author2 |
Amêndola, Isabela Botrel, Tássia Marchetti [UNESP] de OLIVEIRA, Felipe Eduardo Figueira, Leandro Wagner [UNESP] Sper, Fábia Lugli [UNESP] de OLIVEIRA, Jonatas Rafael de OLIVEIRA, Luciane Dias [UNESP] |
author2_role |
author author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade Federal do Rio de Janeiro (UFRJ) Basic Institute of Biosciences Universidade Estadual Paulista (Unesp) Brazcubas Faculty of Dentistry School of Medicine |
dc.contributor.author.fl_str_mv |
Viegas, Daiane de Jesus Amêndola, Isabela Botrel, Tássia Marchetti [UNESP] de OLIVEIRA, Felipe Eduardo Figueira, Leandro Wagner [UNESP] Sper, Fábia Lugli [UNESP] de OLIVEIRA, Jonatas Rafael de OLIVEIRA, Luciane Dias [UNESP] |
dc.subject.por.fl_str_mv |
Biofilms Brazilian peppertree Candida Planktonic cultures Schinus terebinthifolius |
topic |
Biofilms Brazilian peppertree Candida Planktonic cultures Schinus terebinthifolius |
description |
Objective: The use of medicinal plants may be an alternative method for the control of Candida spp. responsible for human infections. This study evaluated the antifungal effect of Schinus terebinthifolius extract (Brazilian Peppertree) on C. albicans, C. dubliniensis, C. glabrata, and C. krusei planktonic cultures and biofilms. Material and Methods: Minimum inhibitory concentration (MIC) and minimum fungal concentration (MFC) of the plant extract were determined by the broth microdilution method. Biofilms formed in microplate wells were exposed to the extract for 5 min (50, 100 and 200 mg/mL) or 24 h (25, 50 and 100 mg/mL). After determination of colony-forming units per milliliter (CFU/mL), the data were analyzed by one-way ANOVA and Tukey’s Test (P ≤ 0.05). Results: Different MIC (mg/mL) were found, such as 0.39 (C. dubliniensis), 1.56 (C. albicans), and 3.13 (C. glabrata and C. krusei). Besides, MFC (mg/mL) of 0.78 (C. dubliniensis) and 3.13 (C. albicans, C. glabrata and C. krusei) were also observed. Regarding the biofilms, significant reductions (log10) were found after 5 min and 24 h exposure to the plant extract, compared to the control group. However, C. dubliniensis was significantly affected only in 24 h treatment. Conclusion: S. terebinthifolius extract presented a significant antifungal effect on C. albicans, C. dubliniensis, C. glabrata, and C. Krusei both in planktonic cultures and biofilms. |
publishDate |
2020 |
dc.date.none.fl_str_mv |
2020-01-01 2021-06-25T10:12:27Z 2021-06-25T10:12:27Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.14295/bds.2020.v23i4.2077 Brazilian Dental Science, v. 23, n. 4, p. 1-7, 2020. 2178-6011 http://hdl.handle.net/11449/205261 10.14295/bds.2020.v23i4.2077 2-s2.0-85092080311 |
url |
http://dx.doi.org/10.14295/bds.2020.v23i4.2077 http://hdl.handle.net/11449/205261 |
identifier_str_mv |
Brazilian Dental Science, v. 23, n. 4, p. 1-7, 2020. 2178-6011 10.14295/bds.2020.v23i4.2077 2-s2.0-85092080311 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Brazilian Dental Science |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
1-7 |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1797790192462987264 |