Influence of puerperium on the serum proteins of Saanen goats, obtained by the analysis of electrophoresis on sodium dodecyl sulphate-polyacrylamide gel
Autor(a) principal: | |
---|---|
Data de Publicação: | 2009 |
Outros Autores: | , , , , , , |
Tipo de documento: | Artigo |
Idioma: | por |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.5433/1679-0359.2009v30n3p661 http://hdl.handle.net/11449/231260 |
Resumo: | Aiming to evaluate the puerperal influence on the proteinogram of Saanen goats, 108 samples of blood serum from 12 goats were collected, and the results were presented at nine times: just after parturition, 1, 3, 5, 7, 10, 15, 21 and 30 days after parturition. Total amount of serum proteins were determined by the biuret technique, and the sodium dodecyl sulphate-polyacrylamide gel electrophoresis (SDS-PAGE) was used to the protein fractionation. In this last method, 17 protein bands were observed, from which molecular weights varied between 25 KDa and 275 KDa. In addition, it was possible to identify the following protein fractions: immunoglobulin A (180 KDa), ceruloplasmin (115 KDa), transferrin(79 KDa), albumin (65 KDa), heavy-chain immunoglobulin G (58 KDa), haptoglobin (45 KDa), acid glycoprotein (37 KDa) and light-chain immunoglobulin G (28 KDa). Another 9 nonidentified protein fractions presented, each molecular weights equal to 275 KDa, 140 KDa, 125 KDa, 103 KDa, 95 KDa, 41 KDa, 35 KDa, 30 Kda and 25 KDa. The results allow us to conclude that by the first week of puerperium, an improvement of acid glycoprotein occurs, whereas those others protein fractions do not suffer any puerperal influence. |
id |
UNSP_00845dd74234e40d267e075fa2264786 |
---|---|
oai_identifier_str |
oai:repositorio.unesp.br:11449/231260 |
network_acronym_str |
UNSP |
network_name_str |
Repositório Institucional da UNESP |
repository_id_str |
2946 |
spelling |
Influence of puerperium on the serum proteins of Saanen goats, obtained by the analysis of electrophoresis on sodium dodecyl sulphate-polyacrylamide gelInfluência do puerpério sobre o proteinograma sérico de caprinos da raça Saanen obtido por eletroforese em gel de poliacrilamidaElectrophoresisGoatsProteinogramPuerperiumSDS-PAGEAiming to evaluate the puerperal influence on the proteinogram of Saanen goats, 108 samples of blood serum from 12 goats were collected, and the results were presented at nine times: just after parturition, 1, 3, 5, 7, 10, 15, 21 and 30 days after parturition. Total amount of serum proteins were determined by the biuret technique, and the sodium dodecyl sulphate-polyacrylamide gel electrophoresis (SDS-PAGE) was used to the protein fractionation. In this last method, 17 protein bands were observed, from which molecular weights varied between 25 KDa and 275 KDa. In addition, it was possible to identify the following protein fractions: immunoglobulin A (180 KDa), ceruloplasmin (115 KDa), transferrin(79 KDa), albumin (65 KDa), heavy-chain immunoglobulin G (58 KDa), haptoglobin (45 KDa), acid glycoprotein (37 KDa) and light-chain immunoglobulin G (28 KDa). Another 9 nonidentified protein fractions presented, each molecular weights equal to 275 KDa, 140 KDa, 125 KDa, 103 KDa, 95 KDa, 41 KDa, 35 KDa, 30 Kda and 25 KDa. The results allow us to conclude that by the first week of puerperium, an improvement of acid glycoprotein occurs, whereas those others protein fractions do not suffer any puerperal influence.Clínica Veterinária da Faculdade de Medicina Veterinária e Zootecnia Universidade de São Paulo FMVZ/USPTécnica Nível Superior da Faculdade de Medicina Veterinária e Zootecnia Universidade de São Paulo - FMVZ/USP.Faculdade de Ciências Agrárias e Veterinárias Universidade Estadual Paulista Júlio de Mesquita Filho UNESPFaculdade de Medicina Veterinária e Zootecnia Universidade de São Paulo FMVZ/USPFaculdade de Ciências Agrárias e Veterinárias Universidade Estadual Paulista Júlio de Mesquita Filho UNESPUniversidade de São Paulo (USP)Universidade Estadual Paulista (UNESP)Saut, João Paulo ElsenDe Souza, Regiane MacHadoBirgel, Daniela BeckerPogliani, Fabio CelidonioCavalcante, Carolina ZaghiMiyashiro, Samantha IveFagliari, José Jurandir [UNESP]Birgel Jr., Eduardo Harry2022-04-29T08:44:24Z2022-04-29T08:44:24Z2009-01-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article661-670http://dx.doi.org/10.5433/1679-0359.2009v30n3p661Semina:Ciencias Agrarias, v. 30, n. 3, p. 661-670, 2009.1679-0359http://hdl.handle.net/11449/23126010.5433/1679-0359.2009v30n3p6612-s2.0-79958702573Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPporSemina:Ciencias Agrariasinfo:eu-repo/semantics/openAccess2022-04-29T08:44:24Zoai:repositorio.unesp.br:11449/231260Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462022-04-29T08:44:24Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Influence of puerperium on the serum proteins of Saanen goats, obtained by the analysis of electrophoresis on sodium dodecyl sulphate-polyacrylamide gel Influência do puerpério sobre o proteinograma sérico de caprinos da raça Saanen obtido por eletroforese em gel de poliacrilamida |
title |
Influence of puerperium on the serum proteins of Saanen goats, obtained by the analysis of electrophoresis on sodium dodecyl sulphate-polyacrylamide gel |
spellingShingle |
Influence of puerperium on the serum proteins of Saanen goats, obtained by the analysis of electrophoresis on sodium dodecyl sulphate-polyacrylamide gel Saut, João Paulo Elsen Electrophoresis Goats Proteinogram Puerperium SDS-PAGE |
title_short |
Influence of puerperium on the serum proteins of Saanen goats, obtained by the analysis of electrophoresis on sodium dodecyl sulphate-polyacrylamide gel |
title_full |
Influence of puerperium on the serum proteins of Saanen goats, obtained by the analysis of electrophoresis on sodium dodecyl sulphate-polyacrylamide gel |
title_fullStr |
Influence of puerperium on the serum proteins of Saanen goats, obtained by the analysis of electrophoresis on sodium dodecyl sulphate-polyacrylamide gel |
title_full_unstemmed |
Influence of puerperium on the serum proteins of Saanen goats, obtained by the analysis of electrophoresis on sodium dodecyl sulphate-polyacrylamide gel |
title_sort |
Influence of puerperium on the serum proteins of Saanen goats, obtained by the analysis of electrophoresis on sodium dodecyl sulphate-polyacrylamide gel |
author |
Saut, João Paulo Elsen |
author_facet |
Saut, João Paulo Elsen De Souza, Regiane MacHado Birgel, Daniela Becker Pogliani, Fabio Celidonio Cavalcante, Carolina Zaghi Miyashiro, Samantha Ive Fagliari, José Jurandir [UNESP] Birgel Jr., Eduardo Harry |
author_role |
author |
author2 |
De Souza, Regiane MacHado Birgel, Daniela Becker Pogliani, Fabio Celidonio Cavalcante, Carolina Zaghi Miyashiro, Samantha Ive Fagliari, José Jurandir [UNESP] Birgel Jr., Eduardo Harry |
author2_role |
author author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade de São Paulo (USP) Universidade Estadual Paulista (UNESP) |
dc.contributor.author.fl_str_mv |
Saut, João Paulo Elsen De Souza, Regiane MacHado Birgel, Daniela Becker Pogliani, Fabio Celidonio Cavalcante, Carolina Zaghi Miyashiro, Samantha Ive Fagliari, José Jurandir [UNESP] Birgel Jr., Eduardo Harry |
dc.subject.por.fl_str_mv |
Electrophoresis Goats Proteinogram Puerperium SDS-PAGE |
topic |
Electrophoresis Goats Proteinogram Puerperium SDS-PAGE |
description |
Aiming to evaluate the puerperal influence on the proteinogram of Saanen goats, 108 samples of blood serum from 12 goats were collected, and the results were presented at nine times: just after parturition, 1, 3, 5, 7, 10, 15, 21 and 30 days after parturition. Total amount of serum proteins were determined by the biuret technique, and the sodium dodecyl sulphate-polyacrylamide gel electrophoresis (SDS-PAGE) was used to the protein fractionation. In this last method, 17 protein bands were observed, from which molecular weights varied between 25 KDa and 275 KDa. In addition, it was possible to identify the following protein fractions: immunoglobulin A (180 KDa), ceruloplasmin (115 KDa), transferrin(79 KDa), albumin (65 KDa), heavy-chain immunoglobulin G (58 KDa), haptoglobin (45 KDa), acid glycoprotein (37 KDa) and light-chain immunoglobulin G (28 KDa). Another 9 nonidentified protein fractions presented, each molecular weights equal to 275 KDa, 140 KDa, 125 KDa, 103 KDa, 95 KDa, 41 KDa, 35 KDa, 30 Kda and 25 KDa. The results allow us to conclude that by the first week of puerperium, an improvement of acid glycoprotein occurs, whereas those others protein fractions do not suffer any puerperal influence. |
publishDate |
2009 |
dc.date.none.fl_str_mv |
2009-01-01 2022-04-29T08:44:24Z 2022-04-29T08:44:24Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.5433/1679-0359.2009v30n3p661 Semina:Ciencias Agrarias, v. 30, n. 3, p. 661-670, 2009. 1679-0359 http://hdl.handle.net/11449/231260 10.5433/1679-0359.2009v30n3p661 2-s2.0-79958702573 |
url |
http://dx.doi.org/10.5433/1679-0359.2009v30n3p661 http://hdl.handle.net/11449/231260 |
identifier_str_mv |
Semina:Ciencias Agrarias, v. 30, n. 3, p. 661-670, 2009. 1679-0359 10.5433/1679-0359.2009v30n3p661 2-s2.0-79958702573 |
dc.language.iso.fl_str_mv |
por |
language |
por |
dc.relation.none.fl_str_mv |
Semina:Ciencias Agrarias |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
661-670 |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1799964928098959360 |