Organic zinc supplementation modifies the metalloproteome of royal jelly produced by Apis mellifera
Autor(a) principal: | |
---|---|
Data de Publicação: | 2021 |
Outros Autores: | , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1080/00218839.2021.1901427 http://hdl.handle.net/11449/208547 |
Resumo: | In this study, we analyzed Apis mellifera royal jelly proteome alterations induced by supplementation with different concentrations of dietary organic zinc (0, 25, 50, and 75 ppm). Proteins extracted from royal jelly were separated by electrophoresis and isoelectric focusing, and zinc-containing protein spots were identified by tandem mass spectrometry. Organic zinc-containing diets modified total royal jelly protein content and altered the expression of five major royal jelly proteins (MRJP1, MRJP2, MRJP3, MRJP5, and MRJP8). While the diet with the lowest tested zinc content (Zn25) increased total royal jelly protein content and expression of MRJP1, diets with higher zinc contents (Zn50 and Zn75) decreased MRJP expression. Our results demonstrate that important proteins and metabolic processes are modified in royal jelly produced by honey bees supplemented with different concentrations of organic zinc source, affecting nutrition and maintenance of colonies. |
id |
UNSP_0089c0ea39171dc0f7799021524867ac |
---|---|
oai_identifier_str |
oai:repositorio.unesp.br:11449/208547 |
network_acronym_str |
UNSP |
network_name_str |
Repositório Institucional da UNESP |
repository_id_str |
2946 |
spelling |
Organic zinc supplementation modifies the metalloproteome of royal jelly produced by Apis melliferaHoney beesmineralprotein expressionsupplementationIn this study, we analyzed Apis mellifera royal jelly proteome alterations induced by supplementation with different concentrations of dietary organic zinc (0, 25, 50, and 75 ppm). Proteins extracted from royal jelly were separated by electrophoresis and isoelectric focusing, and zinc-containing protein spots were identified by tandem mass spectrometry. Organic zinc-containing diets modified total royal jelly protein content and altered the expression of five major royal jelly proteins (MRJP1, MRJP2, MRJP3, MRJP5, and MRJP8). While the diet with the lowest tested zinc content (Zn25) increased total royal jelly protein content and expression of MRJP1, diets with higher zinc contents (Zn50 and Zn75) decreased MRJP expression. Our results demonstrate that important proteins and metabolic processes are modified in royal jelly produced by honey bees supplemented with different concentrations of organic zinc source, affecting nutrition and maintenance of colonies.Center of Education Science and Technology in Rational Beekeeping (NECTAR) College of Veterinary Medicine and Animal Sciences UNESP–São Paulo State UniversityInstitute of Biosciences Department of Biochemistry UNESP - São Paulo State UniversityCenter of Education Science and Technology in Rational Beekeeping (NECTAR) College of Veterinary Medicine and Animal Sciences UNESP–São Paulo State UniversityInstitute of Biosciences Department of Biochemistry UNESP - São Paulo State UniversityUniversidade Estadual Paulista (Unesp)Camilli, Marcelo P. [UNESP]de Barros, Daniel C. B. [UNESP]Martineli, Gabriel M. [UNESP]Longuini, Aimê A. [UNESP]Kadri, Samir Moura [UNESP]Vieira, José Cavalcante S. [UNESP]de Magalhães Padilha, Pedro [UNESP]Orsi, Ricardo de Oliveira [UNESP]2021-06-25T11:13:56Z2021-06-25T11:13:56Z2021-01-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlehttp://dx.doi.org/10.1080/00218839.2021.1901427Journal of Apicultural Research.2078-69130021-8839http://hdl.handle.net/11449/20854710.1080/00218839.2021.19014272-s2.0-85103377080Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengJournal of Apicultural Researchinfo:eu-repo/semantics/openAccess2021-10-23T19:02:16Zoai:repositorio.unesp.br:11449/208547Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462021-10-23T19:02:16Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Organic zinc supplementation modifies the metalloproteome of royal jelly produced by Apis mellifera |
title |
Organic zinc supplementation modifies the metalloproteome of royal jelly produced by Apis mellifera |
spellingShingle |
Organic zinc supplementation modifies the metalloproteome of royal jelly produced by Apis mellifera Camilli, Marcelo P. [UNESP] Honey bees mineral protein expression supplementation |
title_short |
Organic zinc supplementation modifies the metalloproteome of royal jelly produced by Apis mellifera |
title_full |
Organic zinc supplementation modifies the metalloproteome of royal jelly produced by Apis mellifera |
title_fullStr |
Organic zinc supplementation modifies the metalloproteome of royal jelly produced by Apis mellifera |
title_full_unstemmed |
Organic zinc supplementation modifies the metalloproteome of royal jelly produced by Apis mellifera |
title_sort |
Organic zinc supplementation modifies the metalloproteome of royal jelly produced by Apis mellifera |
author |
Camilli, Marcelo P. [UNESP] |
author_facet |
Camilli, Marcelo P. [UNESP] de Barros, Daniel C. B. [UNESP] Martineli, Gabriel M. [UNESP] Longuini, Aimê A. [UNESP] Kadri, Samir Moura [UNESP] Vieira, José Cavalcante S. [UNESP] de Magalhães Padilha, Pedro [UNESP] Orsi, Ricardo de Oliveira [UNESP] |
author_role |
author |
author2 |
de Barros, Daniel C. B. [UNESP] Martineli, Gabriel M. [UNESP] Longuini, Aimê A. [UNESP] Kadri, Samir Moura [UNESP] Vieira, José Cavalcante S. [UNESP] de Magalhães Padilha, Pedro [UNESP] Orsi, Ricardo de Oliveira [UNESP] |
author2_role |
author author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (Unesp) |
dc.contributor.author.fl_str_mv |
Camilli, Marcelo P. [UNESP] de Barros, Daniel C. B. [UNESP] Martineli, Gabriel M. [UNESP] Longuini, Aimê A. [UNESP] Kadri, Samir Moura [UNESP] Vieira, José Cavalcante S. [UNESP] de Magalhães Padilha, Pedro [UNESP] Orsi, Ricardo de Oliveira [UNESP] |
dc.subject.por.fl_str_mv |
Honey bees mineral protein expression supplementation |
topic |
Honey bees mineral protein expression supplementation |
description |
In this study, we analyzed Apis mellifera royal jelly proteome alterations induced by supplementation with different concentrations of dietary organic zinc (0, 25, 50, and 75 ppm). Proteins extracted from royal jelly were separated by electrophoresis and isoelectric focusing, and zinc-containing protein spots were identified by tandem mass spectrometry. Organic zinc-containing diets modified total royal jelly protein content and altered the expression of five major royal jelly proteins (MRJP1, MRJP2, MRJP3, MRJP5, and MRJP8). While the diet with the lowest tested zinc content (Zn25) increased total royal jelly protein content and expression of MRJP1, diets with higher zinc contents (Zn50 and Zn75) decreased MRJP expression. Our results demonstrate that important proteins and metabolic processes are modified in royal jelly produced by honey bees supplemented with different concentrations of organic zinc source, affecting nutrition and maintenance of colonies. |
publishDate |
2021 |
dc.date.none.fl_str_mv |
2021-06-25T11:13:56Z 2021-06-25T11:13:56Z 2021-01-01 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1080/00218839.2021.1901427 Journal of Apicultural Research. 2078-6913 0021-8839 http://hdl.handle.net/11449/208547 10.1080/00218839.2021.1901427 2-s2.0-85103377080 |
url |
http://dx.doi.org/10.1080/00218839.2021.1901427 http://hdl.handle.net/11449/208547 |
identifier_str_mv |
Journal of Apicultural Research. 2078-6913 0021-8839 10.1080/00218839.2021.1901427 2-s2.0-85103377080 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Journal of Apicultural Research |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1799964485107056640 |