Combination of fixative agents and fixation times to visually differentiate the cortical from the medullary layer in bovine adrenal glands
Autor(a) principal: | |
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Data de Publicação: | 2018 |
Outros Autores: | , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1590/0001-3765201820170941 http://hdl.handle.net/11449/188595 |
Resumo: | Chronic stress exposure commonly increases adrenals weight and changes their morphology. This study aimed to compare four methods to delimitate the cortical and medullary layers of adrenals glands in Nelore bulls. Fresh adrenals did not present differentiation between layers. Then, frozen adrenals were distributed in plastics bags with fixative Bouin (G1), 96ºGL ethylic alcohol (G2), 10% formaldehyde (G3), or 2.5% glutaraldehyde (G4). After 12 hours of fixation, the G1 adrenal glands did not show the entire cortical layer marked by Bouin’s solution. For G2 and G3 there was a poor contrast, while for G4 there was a reasonable contrast. After 24 hours of fixation, G1 had an excellent contrast between layers, while G2 and G4 had a reasonable contrast and G3 a very bad contrast. After 48 hours it was difficult to differentiate cortical and medullar layers for G1; for Group 2 we get a reasonable contrast; and for G3 the contrast was bad. For G4 the contrast was not as sharp due to the medulla became dark. It was concluded that fixation of adrenals must be done in Bouin’s solution for 24 hours to obtain an effective evaluation of the adrenals’ morphometry. |
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Combination of fixative agents and fixation times to visually differentiate the cortical from the medullary layer in bovine adrenal glandsAnatomyAnimal welfareGlandMorphometryStressChronic stress exposure commonly increases adrenals weight and changes their morphology. This study aimed to compare four methods to delimitate the cortical and medullary layers of adrenals glands in Nelore bulls. Fresh adrenals did not present differentiation between layers. Then, frozen adrenals were distributed in plastics bags with fixative Bouin (G1), 96ºGL ethylic alcohol (G2), 10% formaldehyde (G3), or 2.5% glutaraldehyde (G4). After 12 hours of fixation, the G1 adrenal glands did not show the entire cortical layer marked by Bouin’s solution. For G2 and G3 there was a poor contrast, while for G4 there was a reasonable contrast. After 24 hours of fixation, G1 had an excellent contrast between layers, while G2 and G4 had a reasonable contrast and G3 a very bad contrast. After 48 hours it was difficult to differentiate cortical and medullar layers for G1; for Group 2 we get a reasonable contrast; and for G3 the contrast was bad. For G4 the contrast was not as sharp due to the medulla became dark. It was concluded that fixation of adrenals must be done in Bouin’s solution for 24 hours to obtain an effective evaluation of the adrenals’ morphometry.Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)Laboratório de Anatomia Cirúrgica Departamento de Morfologia e Fisiologia Animal Faculdade de Ciências Agrárias e Veterinárias Universidade Estadual Paulista/UNESP, Via de acesso Paulo Donato Castelane, s/nInstituto de Ciências Agrárias e Tecnológicas Universidade Federal do Mato Grosso/ UFMT, Avenida dos Estudantes, 5055Departamento de Zootecnia Faculdade de Ciências Agrárias e Veterinárias Universidade Estadual Paulista/UNESP, Via de acesso Paulo Donato Castelane, s/nLaboratório de Anatomia Cirúrgica Departamento de Morfologia e Fisiologia Animal Faculdade de Ciências Agrárias e Veterinárias Universidade Estadual Paulista/UNESP, Via de acesso Paulo Donato Castelane, s/nDepartamento de Zootecnia Faculdade de Ciências Agrárias e Veterinárias Universidade Estadual Paulista/UNESP, Via de acesso Paulo Donato Castelane, s/nFAPESP: 2014/10251-3FAPESP: 2014/10317-4FAPESP: 2015/06353-8CNPq: 483443/2013-1Universidade Estadual Paulista (Unesp)Universidade Federal do Mato Grosso/ UFMTde Oliveira, Fabrício Singaretti [UNESP]Macitelli, FernandaSantos, Caio C. C. [UNESP]Braga, Janaina S. [UNESP]Costa, Barbara C. [UNESP]Pastore, Marina [UNESP]Rocha, Thiago A. S. S. [UNESP]Da Costa, Mateus J. R. P. [UNESP]2019-10-06T16:13:11Z2019-10-06T16:13:11Z2018-10-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article3887-3891application/pdfhttp://dx.doi.org/10.1590/0001-3765201820170941Anais da Academia Brasileira de Ciencias, v. 90, n. 4, p. 3887-3891, 2018.1678-26900001-3765http://hdl.handle.net/11449/18859510.1590/0001-3765201820170941S0001-376520180007038872-s2.0-85059769318S0001-37652018000703887.pdf11855642114387880000-0003-1860-8623Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengAnais da Academia Brasileira de Cienciasinfo:eu-repo/semantics/openAccess2023-10-29T06:13:51Zoai:repositorio.unesp.br:11449/188595Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462023-10-29T06:13:51Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Combination of fixative agents and fixation times to visually differentiate the cortical from the medullary layer in bovine adrenal glands |
title |
Combination of fixative agents and fixation times to visually differentiate the cortical from the medullary layer in bovine adrenal glands |
spellingShingle |
Combination of fixative agents and fixation times to visually differentiate the cortical from the medullary layer in bovine adrenal glands de Oliveira, Fabrício Singaretti [UNESP] Anatomy Animal welfare Gland Morphometry Stress |
title_short |
Combination of fixative agents and fixation times to visually differentiate the cortical from the medullary layer in bovine adrenal glands |
title_full |
Combination of fixative agents and fixation times to visually differentiate the cortical from the medullary layer in bovine adrenal glands |
title_fullStr |
Combination of fixative agents and fixation times to visually differentiate the cortical from the medullary layer in bovine adrenal glands |
title_full_unstemmed |
Combination of fixative agents and fixation times to visually differentiate the cortical from the medullary layer in bovine adrenal glands |
title_sort |
Combination of fixative agents and fixation times to visually differentiate the cortical from the medullary layer in bovine adrenal glands |
author |
de Oliveira, Fabrício Singaretti [UNESP] |
author_facet |
de Oliveira, Fabrício Singaretti [UNESP] Macitelli, Fernanda Santos, Caio C. C. [UNESP] Braga, Janaina S. [UNESP] Costa, Barbara C. [UNESP] Pastore, Marina [UNESP] Rocha, Thiago A. S. S. [UNESP] Da Costa, Mateus J. R. P. [UNESP] |
author_role |
author |
author2 |
Macitelli, Fernanda Santos, Caio C. C. [UNESP] Braga, Janaina S. [UNESP] Costa, Barbara C. [UNESP] Pastore, Marina [UNESP] Rocha, Thiago A. S. S. [UNESP] Da Costa, Mateus J. R. P. [UNESP] |
author2_role |
author author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (Unesp) Universidade Federal do Mato Grosso/ UFMT |
dc.contributor.author.fl_str_mv |
de Oliveira, Fabrício Singaretti [UNESP] Macitelli, Fernanda Santos, Caio C. C. [UNESP] Braga, Janaina S. [UNESP] Costa, Barbara C. [UNESP] Pastore, Marina [UNESP] Rocha, Thiago A. S. S. [UNESP] Da Costa, Mateus J. R. P. [UNESP] |
dc.subject.por.fl_str_mv |
Anatomy Animal welfare Gland Morphometry Stress |
topic |
Anatomy Animal welfare Gland Morphometry Stress |
description |
Chronic stress exposure commonly increases adrenals weight and changes their morphology. This study aimed to compare four methods to delimitate the cortical and medullary layers of adrenals glands in Nelore bulls. Fresh adrenals did not present differentiation between layers. Then, frozen adrenals were distributed in plastics bags with fixative Bouin (G1), 96ºGL ethylic alcohol (G2), 10% formaldehyde (G3), or 2.5% glutaraldehyde (G4). After 12 hours of fixation, the G1 adrenal glands did not show the entire cortical layer marked by Bouin’s solution. For G2 and G3 there was a poor contrast, while for G4 there was a reasonable contrast. After 24 hours of fixation, G1 had an excellent contrast between layers, while G2 and G4 had a reasonable contrast and G3 a very bad contrast. After 48 hours it was difficult to differentiate cortical and medullar layers for G1; for Group 2 we get a reasonable contrast; and for G3 the contrast was bad. For G4 the contrast was not as sharp due to the medulla became dark. It was concluded that fixation of adrenals must be done in Bouin’s solution for 24 hours to obtain an effective evaluation of the adrenals’ morphometry. |
publishDate |
2018 |
dc.date.none.fl_str_mv |
2018-10-01 2019-10-06T16:13:11Z 2019-10-06T16:13:11Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1590/0001-3765201820170941 Anais da Academia Brasileira de Ciencias, v. 90, n. 4, p. 3887-3891, 2018. 1678-2690 0001-3765 http://hdl.handle.net/11449/188595 10.1590/0001-3765201820170941 S0001-37652018000703887 2-s2.0-85059769318 S0001-37652018000703887.pdf 1185564211438788 0000-0003-1860-8623 |
url |
http://dx.doi.org/10.1590/0001-3765201820170941 http://hdl.handle.net/11449/188595 |
identifier_str_mv |
Anais da Academia Brasileira de Ciencias, v. 90, n. 4, p. 3887-3891, 2018. 1678-2690 0001-3765 10.1590/0001-3765201820170941 S0001-37652018000703887 2-s2.0-85059769318 S0001-37652018000703887.pdf 1185564211438788 0000-0003-1860-8623 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Anais da Academia Brasileira de Ciencias |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
3887-3891 application/pdf |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1799964751349940224 |