Cyanotoxin production and phylogeny of benthic cyanobacterial strains isolated from the northeast of Brazil
Autor(a) principal: | |
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Data de Publicação: | 2015 |
Outros Autores: | , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1016/j.hal.2015.01.003 http://hdl.handle.net/11449/220324 |
Resumo: | Most of the knowledge about cyanobacteria toxin production is traditionally associated with planktonic cyanobacterial blooms. However, some studies have been showing that benthic cyanobacteria can produce cyanotoxins. According to this, we aimed to evaluate the production of microcystins and saxitoxins in benthic cyanobacteria isolated from aquatic ecosystems in the Northeast of Brazil and to use a polyphasic approach for their identification. Forty-five clonal strains were isolated from rivers and water supply reservoirs, and identified using morphological and molecular phylogenetic characteristics. In order to evaluate the toxins production, the strains were screened for genes involved in the biosynthesis of microcystins and saxitoxins, positive results were confirmed and cyanotoxins quantified using HPLC. Eight species were identified belonging to the Phormidiaceae, Pseudanabaenaceae and Nostocaceae families. This is the first study in Brazil that shows that strains from the Geitlerinema genus correspond to at least three phylogenetic lineages, which possibly correspond to three distinct species to be subsequently reclassified. The strains that showed one of the genes involved in the cyanotoxins production were analyzed by HPLC and Geitlerinema amphibium, Geitlerinema lemmermannii, Cylindrospermum stagnale and Phormidium uncinatum were identified as producing one or more saxitoxins variants. Thus, this is the first report of saxitoxins production for those first three species and the first report in Brazil for P. uncinatum. |
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Cyanotoxin production and phylogeny of benthic cyanobacterial strains isolated from the northeast of BrazilBenthic cyanobacteriaMicrocystinPhylogenySaxitoxinsSemi-aridMost of the knowledge about cyanobacteria toxin production is traditionally associated with planktonic cyanobacterial blooms. However, some studies have been showing that benthic cyanobacteria can produce cyanotoxins. According to this, we aimed to evaluate the production of microcystins and saxitoxins in benthic cyanobacteria isolated from aquatic ecosystems in the Northeast of Brazil and to use a polyphasic approach for their identification. Forty-five clonal strains were isolated from rivers and water supply reservoirs, and identified using morphological and molecular phylogenetic characteristics. In order to evaluate the toxins production, the strains were screened for genes involved in the biosynthesis of microcystins and saxitoxins, positive results were confirmed and cyanotoxins quantified using HPLC. Eight species were identified belonging to the Phormidiaceae, Pseudanabaenaceae and Nostocaceae families. This is the first study in Brazil that shows that strains from the Geitlerinema genus correspond to at least three phylogenetic lineages, which possibly correspond to three distinct species to be subsequently reclassified. The strains that showed one of the genes involved in the cyanotoxins production were analyzed by HPLC and Geitlerinema amphibium, Geitlerinema lemmermannii, Cylindrospermum stagnale and Phormidium uncinatum were identified as producing one or more saxitoxins variants. Thus, this is the first report of saxitoxins production for those first three species and the first report in Brazil for P. uncinatum.Unidade Acadêmica de Garanhuns Universidade Federal Rural de Pernambuco, Av. Bom Pastor s/nPrograma de Pós-Graduação em Ecologia Universidade Federal Rural de Pernambuco R. D. Manoel de Medeiros, S/N Dois IrmãosUnversidade Estadual Paulista Júlio de Mesquita Filho Departamento de Zoologia e Botânica, R. Cristóvão Colombo 2265Organização Pan-Americana da Saúde (OPAS) Fundacao Nacional de Saude (FUNASA), Av. Conselheiro Rosa e Silva 1489Departamento de Ciências Biológicas Escola Superior de Agricultura Luiz de Queiroz Universidade de São Paulo, Av. Pádua Dias 11Unversidade Estadual Paulista Júlio de Mesquita Filho Departamento de Zoologia e Botânica, R. Cristóvão Colombo 2265Universidade Federal Rural de PernambucoR. D. Manoel de MedeirosUniversidade Estadual Paulista (UNESP)Fundacao Nacional de Saude (FUNASA)Universidade de São Paulo (USP)Borges, H. L.F.Branco, L. H.Z. [UNESP]Martins, M. D. [UNESP]Lima, C. S.Barbosa, P. T.Lira, G. A.S.T.Bittencourt-Oliveira, M. C.Molica, R. J.R.2022-04-28T19:00:55Z2022-04-28T19:00:55Z2015-03-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article46-57http://dx.doi.org/10.1016/j.hal.2015.01.003Harmful Algae, v. 43, p. 46-57.1878-14701568-9883http://hdl.handle.net/11449/22032410.1016/j.hal.2015.01.0032-s2.0-84924420315Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengHarmful Algaeinfo:eu-repo/semantics/openAccess2022-04-28T19:00:55Zoai:repositorio.unesp.br:11449/220324Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462022-04-28T19:00:55Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Cyanotoxin production and phylogeny of benthic cyanobacterial strains isolated from the northeast of Brazil |
title |
Cyanotoxin production and phylogeny of benthic cyanobacterial strains isolated from the northeast of Brazil |
spellingShingle |
Cyanotoxin production and phylogeny of benthic cyanobacterial strains isolated from the northeast of Brazil Borges, H. L.F. Benthic cyanobacteria Microcystin Phylogeny Saxitoxins Semi-arid |
title_short |
Cyanotoxin production and phylogeny of benthic cyanobacterial strains isolated from the northeast of Brazil |
title_full |
Cyanotoxin production and phylogeny of benthic cyanobacterial strains isolated from the northeast of Brazil |
title_fullStr |
Cyanotoxin production and phylogeny of benthic cyanobacterial strains isolated from the northeast of Brazil |
title_full_unstemmed |
Cyanotoxin production and phylogeny of benthic cyanobacterial strains isolated from the northeast of Brazil |
title_sort |
Cyanotoxin production and phylogeny of benthic cyanobacterial strains isolated from the northeast of Brazil |
author |
Borges, H. L.F. |
author_facet |
Borges, H. L.F. Branco, L. H.Z. [UNESP] Martins, M. D. [UNESP] Lima, C. S. Barbosa, P. T. Lira, G. A.S.T. Bittencourt-Oliveira, M. C. Molica, R. J.R. |
author_role |
author |
author2 |
Branco, L. H.Z. [UNESP] Martins, M. D. [UNESP] Lima, C. S. Barbosa, P. T. Lira, G. A.S.T. Bittencourt-Oliveira, M. C. Molica, R. J.R. |
author2_role |
author author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade Federal Rural de Pernambuco R. D. Manoel de Medeiros Universidade Estadual Paulista (UNESP) Fundacao Nacional de Saude (FUNASA) Universidade de São Paulo (USP) |
dc.contributor.author.fl_str_mv |
Borges, H. L.F. Branco, L. H.Z. [UNESP] Martins, M. D. [UNESP] Lima, C. S. Barbosa, P. T. Lira, G. A.S.T. Bittencourt-Oliveira, M. C. Molica, R. J.R. |
dc.subject.por.fl_str_mv |
Benthic cyanobacteria Microcystin Phylogeny Saxitoxins Semi-arid |
topic |
Benthic cyanobacteria Microcystin Phylogeny Saxitoxins Semi-arid |
description |
Most of the knowledge about cyanobacteria toxin production is traditionally associated with planktonic cyanobacterial blooms. However, some studies have been showing that benthic cyanobacteria can produce cyanotoxins. According to this, we aimed to evaluate the production of microcystins and saxitoxins in benthic cyanobacteria isolated from aquatic ecosystems in the Northeast of Brazil and to use a polyphasic approach for their identification. Forty-five clonal strains were isolated from rivers and water supply reservoirs, and identified using morphological and molecular phylogenetic characteristics. In order to evaluate the toxins production, the strains were screened for genes involved in the biosynthesis of microcystins and saxitoxins, positive results were confirmed and cyanotoxins quantified using HPLC. Eight species were identified belonging to the Phormidiaceae, Pseudanabaenaceae and Nostocaceae families. This is the first study in Brazil that shows that strains from the Geitlerinema genus correspond to at least three phylogenetic lineages, which possibly correspond to three distinct species to be subsequently reclassified. The strains that showed one of the genes involved in the cyanotoxins production were analyzed by HPLC and Geitlerinema amphibium, Geitlerinema lemmermannii, Cylindrospermum stagnale and Phormidium uncinatum were identified as producing one or more saxitoxins variants. Thus, this is the first report of saxitoxins production for those first three species and the first report in Brazil for P. uncinatum. |
publishDate |
2015 |
dc.date.none.fl_str_mv |
2015-03-01 2022-04-28T19:00:55Z 2022-04-28T19:00:55Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1016/j.hal.2015.01.003 Harmful Algae, v. 43, p. 46-57. 1878-1470 1568-9883 http://hdl.handle.net/11449/220324 10.1016/j.hal.2015.01.003 2-s2.0-84924420315 |
url |
http://dx.doi.org/10.1016/j.hal.2015.01.003 http://hdl.handle.net/11449/220324 |
identifier_str_mv |
Harmful Algae, v. 43, p. 46-57. 1878-1470 1568-9883 10.1016/j.hal.2015.01.003 2-s2.0-84924420315 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Harmful Algae |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
46-57 |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
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1797790425913753600 |