Effect of passive ultrasonic activation on microorganisms in primary root canal infection: A randomized clinical trial
Autor(a) principal: | |
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Data de Publicação: | 2020 |
Outros Autores: | , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1590/1678-7757-2019-0100 http://hdl.handle.net/11449/201379 |
Resumo: | Objective: This clinical study sought to evaluate the effectiveness of passive ultrasonic activation (PUA) in eliminating microorganisms in primary endodontic infection (PEI) after instrumentation of root canals using microbiological culture and checkerboard DNA-DNA hybridization. Methodology: Twenty root canals with PEI and apical periodontitis were selected. The root canals were instrumented and then randomly divided into 2 groups, according to the irrigation method: PUA and conventional needle irrigation (CNI). Microbiological samples were collected before instrumentation (S1), after instrumentation (S2) and after irrigation with 17% EDTA (S3). The samples were subjected to anaerobic culture technique and checkerboard DNA-DNA hybridization analysis. Results: A statistically significant difference was found between CNI (23.56%) and PUA (98.37%) regarding the median percentage values for culturable bacteria reduction (p<0.05). In the initial samples, the most frequently detected species was S. constellatus (50%), and after root canal treatment was E. faecalis (50%). Conclusion: Both treatments significantly decreased the number of bacterial species compared with the initial sample. However, no statistical difference in the total microbial load between PUA and CNI groups was detected. The number of cultivable anaerobic bacteria reduced significantly using PUA, and the bacterial composition and number of bacterial species after using either CNI or PUA was similar. |
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Repositório Institucional da UNESP |
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Effect of passive ultrasonic activation on microorganisms in primary root canal infection: A randomized clinical trialBacteriaRoot canal therapyUltrasoundObjective: This clinical study sought to evaluate the effectiveness of passive ultrasonic activation (PUA) in eliminating microorganisms in primary endodontic infection (PEI) after instrumentation of root canals using microbiological culture and checkerboard DNA-DNA hybridization. Methodology: Twenty root canals with PEI and apical periodontitis were selected. The root canals were instrumented and then randomly divided into 2 groups, according to the irrigation method: PUA and conventional needle irrigation (CNI). Microbiological samples were collected before instrumentation (S1), after instrumentation (S2) and after irrigation with 17% EDTA (S3). The samples were subjected to anaerobic culture technique and checkerboard DNA-DNA hybridization analysis. Results: A statistically significant difference was found between CNI (23.56%) and PUA (98.37%) regarding the median percentage values for culturable bacteria reduction (p<0.05). In the initial samples, the most frequently detected species was S. constellatus (50%), and after root canal treatment was E. faecalis (50%). Conclusion: Both treatments significantly decreased the number of bacterial species compared with the initial sample. However, no statistical difference in the total microbial load between PUA and CNI groups was detected. The number of cultivable anaerobic bacteria reduced significantly using PUA, and the bacterial composition and number of bacterial species after using either CNI or PUA was similar.Universidade Estadual Paulista (UNESP) Instituto de Ciências e Tecnologia Departamento de Odontologia RestauradoraUniversidade Estadual Paulista (UNESP) Instituto de Ciências e Tecnologia Departamento de Odontologia RestauradoraUniversidade Estadual Paulista (Unesp)Orozco, Esteban Isai Flores [UNESP]Toia, Cassia Cestari [UNESP]Cavalli, Daiana [UNESP]Khoury, Rayana Duarte [UNESP]Cardoso, Flávia Goulart da Rosa [UNESP]Bresciani, Eduardo [UNESP]Valera, Marcia Carneiro [UNESP]2020-12-12T02:31:02Z2020-12-12T02:31:02Z2020-01-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://dx.doi.org/10.1590/1678-7757-2019-0100Journal of Applied Oral Science, v. 28.1678-77651678-7757http://hdl.handle.net/11449/20137910.1590/1678-7757-2019-0100S1678-775720200001004102-s2.0-85075980655S1678-77572020000100410.pdfScopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengJournal of Applied Oral Scienceinfo:eu-repo/semantics/openAccess2023-12-05T06:18:07Zoai:repositorio.unesp.br:11449/201379Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462023-12-05T06:18:07Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Effect of passive ultrasonic activation on microorganisms in primary root canal infection: A randomized clinical trial |
title |
Effect of passive ultrasonic activation on microorganisms in primary root canal infection: A randomized clinical trial |
spellingShingle |
Effect of passive ultrasonic activation on microorganisms in primary root canal infection: A randomized clinical trial Orozco, Esteban Isai Flores [UNESP] Bacteria Root canal therapy Ultrasound |
title_short |
Effect of passive ultrasonic activation on microorganisms in primary root canal infection: A randomized clinical trial |
title_full |
Effect of passive ultrasonic activation on microorganisms in primary root canal infection: A randomized clinical trial |
title_fullStr |
Effect of passive ultrasonic activation on microorganisms in primary root canal infection: A randomized clinical trial |
title_full_unstemmed |
Effect of passive ultrasonic activation on microorganisms in primary root canal infection: A randomized clinical trial |
title_sort |
Effect of passive ultrasonic activation on microorganisms in primary root canal infection: A randomized clinical trial |
author |
Orozco, Esteban Isai Flores [UNESP] |
author_facet |
Orozco, Esteban Isai Flores [UNESP] Toia, Cassia Cestari [UNESP] Cavalli, Daiana [UNESP] Khoury, Rayana Duarte [UNESP] Cardoso, Flávia Goulart da Rosa [UNESP] Bresciani, Eduardo [UNESP] Valera, Marcia Carneiro [UNESP] |
author_role |
author |
author2 |
Toia, Cassia Cestari [UNESP] Cavalli, Daiana [UNESP] Khoury, Rayana Duarte [UNESP] Cardoso, Flávia Goulart da Rosa [UNESP] Bresciani, Eduardo [UNESP] Valera, Marcia Carneiro [UNESP] |
author2_role |
author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (Unesp) |
dc.contributor.author.fl_str_mv |
Orozco, Esteban Isai Flores [UNESP] Toia, Cassia Cestari [UNESP] Cavalli, Daiana [UNESP] Khoury, Rayana Duarte [UNESP] Cardoso, Flávia Goulart da Rosa [UNESP] Bresciani, Eduardo [UNESP] Valera, Marcia Carneiro [UNESP] |
dc.subject.por.fl_str_mv |
Bacteria Root canal therapy Ultrasound |
topic |
Bacteria Root canal therapy Ultrasound |
description |
Objective: This clinical study sought to evaluate the effectiveness of passive ultrasonic activation (PUA) in eliminating microorganisms in primary endodontic infection (PEI) after instrumentation of root canals using microbiological culture and checkerboard DNA-DNA hybridization. Methodology: Twenty root canals with PEI and apical periodontitis were selected. The root canals were instrumented and then randomly divided into 2 groups, according to the irrigation method: PUA and conventional needle irrigation (CNI). Microbiological samples were collected before instrumentation (S1), after instrumentation (S2) and after irrigation with 17% EDTA (S3). The samples were subjected to anaerobic culture technique and checkerboard DNA-DNA hybridization analysis. Results: A statistically significant difference was found between CNI (23.56%) and PUA (98.37%) regarding the median percentage values for culturable bacteria reduction (p<0.05). In the initial samples, the most frequently detected species was S. constellatus (50%), and after root canal treatment was E. faecalis (50%). Conclusion: Both treatments significantly decreased the number of bacterial species compared with the initial sample. However, no statistical difference in the total microbial load between PUA and CNI groups was detected. The number of cultivable anaerobic bacteria reduced significantly using PUA, and the bacterial composition and number of bacterial species after using either CNI or PUA was similar. |
publishDate |
2020 |
dc.date.none.fl_str_mv |
2020-12-12T02:31:02Z 2020-12-12T02:31:02Z 2020-01-01 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1590/1678-7757-2019-0100 Journal of Applied Oral Science, v. 28. 1678-7765 1678-7757 http://hdl.handle.net/11449/201379 10.1590/1678-7757-2019-0100 S1678-77572020000100410 2-s2.0-85075980655 S1678-77572020000100410.pdf |
url |
http://dx.doi.org/10.1590/1678-7757-2019-0100 http://hdl.handle.net/11449/201379 |
identifier_str_mv |
Journal of Applied Oral Science, v. 28. 1678-7765 1678-7757 10.1590/1678-7757-2019-0100 S1678-77572020000100410 2-s2.0-85075980655 S1678-77572020000100410.pdf |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Journal of Applied Oral Science |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1797789911017848832 |