A perspective of mitochondrial dysfunction in rats treated with silver and titanium nanoparticles (AgNPs and TiNPs)
Autor(a) principal: | |
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Data de Publicação: | 2018 |
Outros Autores: | , , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1016/j.jtemb.2018.01.007 http://hdl.handle.net/11449/177092 |
Resumo: | Nanotechnology is a growing branch of science that deals with the development of structural features bearing at least one dimension in the nano range. More specifically, nanomaterials are defined as objects with dimensions that range from 1 to 100 nm, which give rise to interesting properties. In particular, silver and titanium nanoparticles (AgNPs and TiNPs, respectively) are known for their biological and biomedical properties and are often used in consumer products such as cosmetics, food additives, kitchen utensils, and toys. This situation has increased environmental and occupational exposure to AgNPs and TiNPs, which has placed demand for the risk assessment of NPs. Indeed, the same properties that make nanomaterials so attractive could also prove deleterious to biological systems. Of particular concern is the effect of NPs on mitochondria because these organelles play an essential role in cellular homeostasis. In this scenario, this work aimed to study how AgNPs and TiNPs interact with the mitochondrial respiration chain and to analyze how this interaction interferes in the bioenergetics and oxidative state of the organelles after sub-chronic exposure. Mitochondria were exposed to the NPs by gavage treatment for 21 days to check whether co-exposure of the organelles to the two types of NPs elicited any mitochondrion-NP interaction. More specifically, male Wistar rats were randomly assigned to four groups. Groups I, II, III, and IV received mineral oil, TiNPs (100 μg/kg/day), AgNPs (100 μg/kg/day), and TiNPs + AgNPs (100 μg/kg/day), respectively, by gavage. The liver was immediately removed, and the mitochondria were isolated and used within 3 h. Exposure of mitochondria to TiNPs + AgNPs lowered the respiratory control ratio, causing an uncoupling effect in the oxidative phosphorylation system. Moreover, both types of NPs induced mitochondrial swelling. Extended exposure of mitochondria to the NPs maintained increased ROS levels and depleted the endogenous antioxidant system. The AgNPs and TiNPs acted synergistically—the intensity of the toxic effect on the mitochondrial redox state was more significant in the presence of both types of NPs. These findings imply that the action of the NPs on mitochondria underlie NP toxicity, so future application of NPs requires special attention. |
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A perspective of mitochondrial dysfunction in rats treated with silver and titanium nanoparticles (AgNPs and TiNPs)MitotoxicantsNanomaterialsSilver nanoparticlesTitanium nanoparticlesNanotechnology is a growing branch of science that deals with the development of structural features bearing at least one dimension in the nano range. More specifically, nanomaterials are defined as objects with dimensions that range from 1 to 100 nm, which give rise to interesting properties. In particular, silver and titanium nanoparticles (AgNPs and TiNPs, respectively) are known for their biological and biomedical properties and are often used in consumer products such as cosmetics, food additives, kitchen utensils, and toys. This situation has increased environmental and occupational exposure to AgNPs and TiNPs, which has placed demand for the risk assessment of NPs. Indeed, the same properties that make nanomaterials so attractive could also prove deleterious to biological systems. Of particular concern is the effect of NPs on mitochondria because these organelles play an essential role in cellular homeostasis. In this scenario, this work aimed to study how AgNPs and TiNPs interact with the mitochondrial respiration chain and to analyze how this interaction interferes in the bioenergetics and oxidative state of the organelles after sub-chronic exposure. Mitochondria were exposed to the NPs by gavage treatment for 21 days to check whether co-exposure of the organelles to the two types of NPs elicited any mitochondrion-NP interaction. More specifically, male Wistar rats were randomly assigned to four groups. Groups I, II, III, and IV received mineral oil, TiNPs (100 μg/kg/day), AgNPs (100 μg/kg/day), and TiNPs + AgNPs (100 μg/kg/day), respectively, by gavage. The liver was immediately removed, and the mitochondria were isolated and used within 3 h. Exposure of mitochondria to TiNPs + AgNPs lowered the respiratory control ratio, causing an uncoupling effect in the oxidative phosphorylation system. Moreover, both types of NPs induced mitochondrial swelling. Extended exposure of mitochondria to the NPs maintained increased ROS levels and depleted the endogenous antioxidant system. The AgNPs and TiNPs acted synergistically—the intensity of the toxic effect on the mitochondrial redox state was more significant in the presence of both types of NPs. These findings imply that the action of the NPs on mitochondria underlie NP toxicity, so future application of NPs requires special attention.Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Faculdade de Ciências Farmacêuticas de Ribeirão Preto Departamento de Análises Clínicas Toxicológicas e Bromatológicas Universidade de São Paulo, Av. Bandeirantes, 3900, Bairro Monte AlegreDepartamento Bioprocessos e Biotecnologia Faculdade de Ciências Agronômicas Fazenda Experimental de Lageado Universidade Estadual Paulista “Júlio de Mesquita Filho”Instituto de Ciência Engenharia e Tecnologia Universidade Federal dos Vales do Jequitinhonha e MucuriFaculdade de Filosofia Ciências e Letras de Ribeirão Preto Departamento de Química Universidade de São Paulo, Avenida do Café s/n°National Institute for Alternative Technologies of Detection Toxicological Evaluation and Removal of Micropollutants and Radioactives (INCT-DATREM) Unesp Institute of Chemistry, P.O. Box 355Departamento Bioprocessos e Biotecnologia Faculdade de Ciências Agronômicas Fazenda Experimental de Lageado Universidade Estadual Paulista “Júlio de Mesquita Filho”National Institute for Alternative Technologies of Detection Toxicological Evaluation and Removal of Micropollutants and Radioactives (INCT-DATREM) Unesp Institute of Chemistry, P.O. Box 355Universidade de São Paulo (USP)Universidade Estadual Paulista (Unesp)Universidade Federal dos Vales do Jequitinhonha e MucuriPereira, Lilian Cristina [UNESP]Pazin, MuriloFranco-Bernardes, Mariana FurioMartins, Airton da CunhaBarcelos, Gustavo Rafael MazzaronPereira, Márcio CesarMesquita, João PauloRodrigues, Jairo LisboaBarbosa, FernandoDorta, Daniel Junqueira [UNESP]2018-12-11T17:23:57Z2018-12-11T17:23:57Z2018-05-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article63-69application/pdfhttp://dx.doi.org/10.1016/j.jtemb.2018.01.007Journal of Trace Elements in Medicine and Biology, v. 47, p. 63-69.1878-32520946-672Xhttp://hdl.handle.net/11449/17709210.1016/j.jtemb.2018.01.0072-s2.0-850416614032-s2.0-85041661403.pdfScopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengJournal of Trace Elements in Medicine and Biologyinfo:eu-repo/semantics/openAccess2024-01-15T06:23:28Zoai:repositorio.unesp.br:11449/177092Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-01-15T06:23:28Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
A perspective of mitochondrial dysfunction in rats treated with silver and titanium nanoparticles (AgNPs and TiNPs) |
title |
A perspective of mitochondrial dysfunction in rats treated with silver and titanium nanoparticles (AgNPs and TiNPs) |
spellingShingle |
A perspective of mitochondrial dysfunction in rats treated with silver and titanium nanoparticles (AgNPs and TiNPs) Pereira, Lilian Cristina [UNESP] Mitotoxicants Nanomaterials Silver nanoparticles Titanium nanoparticles |
title_short |
A perspective of mitochondrial dysfunction in rats treated with silver and titanium nanoparticles (AgNPs and TiNPs) |
title_full |
A perspective of mitochondrial dysfunction in rats treated with silver and titanium nanoparticles (AgNPs and TiNPs) |
title_fullStr |
A perspective of mitochondrial dysfunction in rats treated with silver and titanium nanoparticles (AgNPs and TiNPs) |
title_full_unstemmed |
A perspective of mitochondrial dysfunction in rats treated with silver and titanium nanoparticles (AgNPs and TiNPs) |
title_sort |
A perspective of mitochondrial dysfunction in rats treated with silver and titanium nanoparticles (AgNPs and TiNPs) |
author |
Pereira, Lilian Cristina [UNESP] |
author_facet |
Pereira, Lilian Cristina [UNESP] Pazin, Murilo Franco-Bernardes, Mariana Furio Martins, Airton da Cunha Barcelos, Gustavo Rafael Mazzaron Pereira, Márcio Cesar Mesquita, João Paulo Rodrigues, Jairo Lisboa Barbosa, Fernando Dorta, Daniel Junqueira [UNESP] |
author_role |
author |
author2 |
Pazin, Murilo Franco-Bernardes, Mariana Furio Martins, Airton da Cunha Barcelos, Gustavo Rafael Mazzaron Pereira, Márcio Cesar Mesquita, João Paulo Rodrigues, Jairo Lisboa Barbosa, Fernando Dorta, Daniel Junqueira [UNESP] |
author2_role |
author author author author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade de São Paulo (USP) Universidade Estadual Paulista (Unesp) Universidade Federal dos Vales do Jequitinhonha e Mucuri |
dc.contributor.author.fl_str_mv |
Pereira, Lilian Cristina [UNESP] Pazin, Murilo Franco-Bernardes, Mariana Furio Martins, Airton da Cunha Barcelos, Gustavo Rafael Mazzaron Pereira, Márcio Cesar Mesquita, João Paulo Rodrigues, Jairo Lisboa Barbosa, Fernando Dorta, Daniel Junqueira [UNESP] |
dc.subject.por.fl_str_mv |
Mitotoxicants Nanomaterials Silver nanoparticles Titanium nanoparticles |
topic |
Mitotoxicants Nanomaterials Silver nanoparticles Titanium nanoparticles |
description |
Nanotechnology is a growing branch of science that deals with the development of structural features bearing at least one dimension in the nano range. More specifically, nanomaterials are defined as objects with dimensions that range from 1 to 100 nm, which give rise to interesting properties. In particular, silver and titanium nanoparticles (AgNPs and TiNPs, respectively) are known for their biological and biomedical properties and are often used in consumer products such as cosmetics, food additives, kitchen utensils, and toys. This situation has increased environmental and occupational exposure to AgNPs and TiNPs, which has placed demand for the risk assessment of NPs. Indeed, the same properties that make nanomaterials so attractive could also prove deleterious to biological systems. Of particular concern is the effect of NPs on mitochondria because these organelles play an essential role in cellular homeostasis. In this scenario, this work aimed to study how AgNPs and TiNPs interact with the mitochondrial respiration chain and to analyze how this interaction interferes in the bioenergetics and oxidative state of the organelles after sub-chronic exposure. Mitochondria were exposed to the NPs by gavage treatment for 21 days to check whether co-exposure of the organelles to the two types of NPs elicited any mitochondrion-NP interaction. More specifically, male Wistar rats were randomly assigned to four groups. Groups I, II, III, and IV received mineral oil, TiNPs (100 μg/kg/day), AgNPs (100 μg/kg/day), and TiNPs + AgNPs (100 μg/kg/day), respectively, by gavage. The liver was immediately removed, and the mitochondria were isolated and used within 3 h. Exposure of mitochondria to TiNPs + AgNPs lowered the respiratory control ratio, causing an uncoupling effect in the oxidative phosphorylation system. Moreover, both types of NPs induced mitochondrial swelling. Extended exposure of mitochondria to the NPs maintained increased ROS levels and depleted the endogenous antioxidant system. The AgNPs and TiNPs acted synergistically—the intensity of the toxic effect on the mitochondrial redox state was more significant in the presence of both types of NPs. These findings imply that the action of the NPs on mitochondria underlie NP toxicity, so future application of NPs requires special attention. |
publishDate |
2018 |
dc.date.none.fl_str_mv |
2018-12-11T17:23:57Z 2018-12-11T17:23:57Z 2018-05-01 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1016/j.jtemb.2018.01.007 Journal of Trace Elements in Medicine and Biology, v. 47, p. 63-69. 1878-3252 0946-672X http://hdl.handle.net/11449/177092 10.1016/j.jtemb.2018.01.007 2-s2.0-85041661403 2-s2.0-85041661403.pdf |
url |
http://dx.doi.org/10.1016/j.jtemb.2018.01.007 http://hdl.handle.net/11449/177092 |
identifier_str_mv |
Journal of Trace Elements in Medicine and Biology, v. 47, p. 63-69. 1878-3252 0946-672X 10.1016/j.jtemb.2018.01.007 2-s2.0-85041661403 2-s2.0-85041661403.pdf |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Journal of Trace Elements in Medicine and Biology |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
63-69 application/pdf |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
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1797790303483068416 |