Ovarian contrast-enhanced ultrasonography and Doppler fluxometry in bitches during the postovulatory estrus and corpora lutea formation
Autor(a) principal: | |
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Data de Publicação: | 2022 |
Outros Autores: | , , , , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1016/j.theriogenology.2022.10.009 http://hdl.handle.net/11449/247738 |
Resumo: | The aim of the present study was to evaluate the vascularization features of canine ovaries during the follicular phase and the formation of the corpora lutea by using Doppler ultrasonography and Contrast-Enhanced Ultrasound (CEUS). Eight healthy bitches were enrolled in the study and were evaluated at five different timepoints (T1 – T5) of the estrous cycle established by vaginal cytology and serum progesterone concentration. Ultrasonographic examinations were performed by a single operator using the ACUSON S2000/SIEMENS machine equipped with a linear multifrequency transducer (9.0 MHz). Color-coded Doppler evaluation of the ovarian parenchyma was performed to investigate the aspects of the signal detection throughout the different timepoints. Pulsed-wave Doppler of the intraovarian arteries was performed to evaluate spectral waveform and doppler velocimetric parameters of Systolic Peak Velocity (SPV cm/s), End Diastolic Velocity (EDV cm/s), Resistivity Index (RI) and Pulsatility Index (PI). CEUS evaluation of the ovaries was performed using a vascular contrast agent (SonoVue®, Bracco, Sao Paulo, Brazil) and the CADENCE™ Contrast Pulse Sequencing (CPS, Siemens) software, in order to perform both qualitative and quantitative analysis. Perfusion parameters of pixel number, peak intensity (PPI in %), time to peak intensity (TTP in s), mean transit time (MTT in s) and area under the curve (AUC in %). Colour-coded Doppler evaluation demonstrated an increase in signal detection as cycle progressed, with blood flow initially detected with few coloured pixels and mainly at the ventral surface of the ovaries. Further on, the number of coloured pixels increased and spreading to the central region, resulting in a circular-like pattern around the corpora hemorrhagica. The spectral waveform was consistent at all timepoints. SPV (cm/s) and EDV (cm/s) presented a numeric trend and a slight statistical difference at all timepoints, whereas no difference was found for RI and PI. CEUS evaluation demonstrated an increase in pixel intensity across all the timepoints. Quantitative CEUS analysis revealed a statistical difference in PPI (%), MTT (s) and AUC (%) at T5. CEUS evaluation of the ovaries was feasible and demonstrated a marked increase in perfusion parameters in the late postovulatory period, demonstrating its applicability in the assessment of canine corpora lutea development. |
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Ovarian contrast-enhanced ultrasonography and Doppler fluxometry in bitches during the postovulatory estrus and corpora lutea formationCanine. ovarian perfusion. ovarian follicle. corpus luteum. angiogenesisThe aim of the present study was to evaluate the vascularization features of canine ovaries during the follicular phase and the formation of the corpora lutea by using Doppler ultrasonography and Contrast-Enhanced Ultrasound (CEUS). Eight healthy bitches were enrolled in the study and were evaluated at five different timepoints (T1 – T5) of the estrous cycle established by vaginal cytology and serum progesterone concentration. Ultrasonographic examinations were performed by a single operator using the ACUSON S2000/SIEMENS machine equipped with a linear multifrequency transducer (9.0 MHz). Color-coded Doppler evaluation of the ovarian parenchyma was performed to investigate the aspects of the signal detection throughout the different timepoints. Pulsed-wave Doppler of the intraovarian arteries was performed to evaluate spectral waveform and doppler velocimetric parameters of Systolic Peak Velocity (SPV cm/s), End Diastolic Velocity (EDV cm/s), Resistivity Index (RI) and Pulsatility Index (PI). CEUS evaluation of the ovaries was performed using a vascular contrast agent (SonoVue®, Bracco, Sao Paulo, Brazil) and the CADENCE™ Contrast Pulse Sequencing (CPS, Siemens) software, in order to perform both qualitative and quantitative analysis. Perfusion parameters of pixel number, peak intensity (PPI in %), time to peak intensity (TTP in s), mean transit time (MTT in s) and area under the curve (AUC in %). Colour-coded Doppler evaluation demonstrated an increase in signal detection as cycle progressed, with blood flow initially detected with few coloured pixels and mainly at the ventral surface of the ovaries. Further on, the number of coloured pixels increased and spreading to the central region, resulting in a circular-like pattern around the corpora hemorrhagica. The spectral waveform was consistent at all timepoints. SPV (cm/s) and EDV (cm/s) presented a numeric trend and a slight statistical difference at all timepoints, whereas no difference was found for RI and PI. CEUS evaluation demonstrated an increase in pixel intensity across all the timepoints. Quantitative CEUS analysis revealed a statistical difference in PPI (%), MTT (s) and AUC (%) at T5. CEUS evaluation of the ovaries was feasible and demonstrated a marked increase in perfusion parameters in the late postovulatory period, demonstrating its applicability in the assessment of canine corpora lutea development.Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Department of Pathology Reproduction and One Health School of Agricultural and Veterinarian Sciences Sao Paulo State University “Julio de Mesquita Filho” (FCAV/UNESP), Sao PauloDepartment of Veterinary Clinic and Surgery School of Agricultural and Veterinarian Sciences Sao Paulo State University “Júlio de Mesquita Filho” (FCAV/UNESP), Sao PauloMarilia University (UNIMAR), Sao PauloResearch Group INCA-CES Faculty of Veterinary Medicine and Animal Science Universidad CESDepartment of Veterinary Medicine and Animal Productions University of Naples “Federico II”Laboratory of Veterinary Imaginology Faculty of Animal Science and Food Engineering (FZEA) Sao Paulo University (USP), Sao PauloDepartment of Pathology Reproduction and One Health School of Agricultural and Veterinarian Sciences Sao Paulo State University “Julio de Mesquita Filho” (FCAV/UNESP), Sao PauloDepartment of Veterinary Clinic and Surgery School of Agricultural and Veterinarian Sciences Sao Paulo State University “Júlio de Mesquita Filho” (FCAV/UNESP), Sao PauloCNPq: nº 130820/2019-9FAPESP: nº 2017/14957-6FAPESP: nº 2019/15282-8CNPq: nº 309199/2017-4CNPq: nº 430023/2018-9Universidade Estadual Paulista (UNESP)Marilia University (UNIMAR)Universidad CESUniversity of Naples “Federico II”Universidade de São Paulo (USP)Nogueira Aires, Luiz Paulo [UNESP]Gasser, Beatriz [UNESP]Silva, Priscila [UNESP]Del'Aguila-Silva, Priscila [UNESP]Yamada, Diego IwaoCarneiro, Rafael Kretzer [UNESP]Bruna Bressianini Lima, Bruna [UNESP]Padilha-Nakaghi, Luciana Cristina [UNESP]Ramirez Uscategui, Ricardo AndrésSpada, StefanoRusso, MarcoRossi Feliciano, Marcus Antônio [UNESP]2023-07-29T13:24:25Z2023-07-29T13:24:25Z2022-12-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article162-170http://dx.doi.org/10.1016/j.theriogenology.2022.10.009Theriogenology, v. 194, p. 162-170.0093-691Xhttp://hdl.handle.net/11449/24773810.1016/j.theriogenology.2022.10.0092-s2.0-85139831028Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengTheriogenologyinfo:eu-repo/semantics/openAccess2023-07-29T13:24:25Zoai:repositorio.unesp.br:11449/247738Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462023-07-29T13:24:25Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Ovarian contrast-enhanced ultrasonography and Doppler fluxometry in bitches during the postovulatory estrus and corpora lutea formation |
title |
Ovarian contrast-enhanced ultrasonography and Doppler fluxometry in bitches during the postovulatory estrus and corpora lutea formation |
spellingShingle |
Ovarian contrast-enhanced ultrasonography and Doppler fluxometry in bitches during the postovulatory estrus and corpora lutea formation Nogueira Aires, Luiz Paulo [UNESP] Canine. ovarian perfusion. ovarian follicle. corpus luteum. angiogenesis |
title_short |
Ovarian contrast-enhanced ultrasonography and Doppler fluxometry in bitches during the postovulatory estrus and corpora lutea formation |
title_full |
Ovarian contrast-enhanced ultrasonography and Doppler fluxometry in bitches during the postovulatory estrus and corpora lutea formation |
title_fullStr |
Ovarian contrast-enhanced ultrasonography and Doppler fluxometry in bitches during the postovulatory estrus and corpora lutea formation |
title_full_unstemmed |
Ovarian contrast-enhanced ultrasonography and Doppler fluxometry in bitches during the postovulatory estrus and corpora lutea formation |
title_sort |
Ovarian contrast-enhanced ultrasonography and Doppler fluxometry in bitches during the postovulatory estrus and corpora lutea formation |
author |
Nogueira Aires, Luiz Paulo [UNESP] |
author_facet |
Nogueira Aires, Luiz Paulo [UNESP] Gasser, Beatriz [UNESP] Silva, Priscila [UNESP] Del'Aguila-Silva, Priscila [UNESP] Yamada, Diego Iwao Carneiro, Rafael Kretzer [UNESP] Bruna Bressianini Lima, Bruna [UNESP] Padilha-Nakaghi, Luciana Cristina [UNESP] Ramirez Uscategui, Ricardo Andrés Spada, Stefano Russo, Marco Rossi Feliciano, Marcus Antônio [UNESP] |
author_role |
author |
author2 |
Gasser, Beatriz [UNESP] Silva, Priscila [UNESP] Del'Aguila-Silva, Priscila [UNESP] Yamada, Diego Iwao Carneiro, Rafael Kretzer [UNESP] Bruna Bressianini Lima, Bruna [UNESP] Padilha-Nakaghi, Luciana Cristina [UNESP] Ramirez Uscategui, Ricardo Andrés Spada, Stefano Russo, Marco Rossi Feliciano, Marcus Antônio [UNESP] |
author2_role |
author author author author author author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (UNESP) Marilia University (UNIMAR) Universidad CES University of Naples “Federico II” Universidade de São Paulo (USP) |
dc.contributor.author.fl_str_mv |
Nogueira Aires, Luiz Paulo [UNESP] Gasser, Beatriz [UNESP] Silva, Priscila [UNESP] Del'Aguila-Silva, Priscila [UNESP] Yamada, Diego Iwao Carneiro, Rafael Kretzer [UNESP] Bruna Bressianini Lima, Bruna [UNESP] Padilha-Nakaghi, Luciana Cristina [UNESP] Ramirez Uscategui, Ricardo Andrés Spada, Stefano Russo, Marco Rossi Feliciano, Marcus Antônio [UNESP] |
dc.subject.por.fl_str_mv |
Canine. ovarian perfusion. ovarian follicle. corpus luteum. angiogenesis |
topic |
Canine. ovarian perfusion. ovarian follicle. corpus luteum. angiogenesis |
description |
The aim of the present study was to evaluate the vascularization features of canine ovaries during the follicular phase and the formation of the corpora lutea by using Doppler ultrasonography and Contrast-Enhanced Ultrasound (CEUS). Eight healthy bitches were enrolled in the study and were evaluated at five different timepoints (T1 – T5) of the estrous cycle established by vaginal cytology and serum progesterone concentration. Ultrasonographic examinations were performed by a single operator using the ACUSON S2000/SIEMENS machine equipped with a linear multifrequency transducer (9.0 MHz). Color-coded Doppler evaluation of the ovarian parenchyma was performed to investigate the aspects of the signal detection throughout the different timepoints. Pulsed-wave Doppler of the intraovarian arteries was performed to evaluate spectral waveform and doppler velocimetric parameters of Systolic Peak Velocity (SPV cm/s), End Diastolic Velocity (EDV cm/s), Resistivity Index (RI) and Pulsatility Index (PI). CEUS evaluation of the ovaries was performed using a vascular contrast agent (SonoVue®, Bracco, Sao Paulo, Brazil) and the CADENCE™ Contrast Pulse Sequencing (CPS, Siemens) software, in order to perform both qualitative and quantitative analysis. Perfusion parameters of pixel number, peak intensity (PPI in %), time to peak intensity (TTP in s), mean transit time (MTT in s) and area under the curve (AUC in %). Colour-coded Doppler evaluation demonstrated an increase in signal detection as cycle progressed, with blood flow initially detected with few coloured pixels and mainly at the ventral surface of the ovaries. Further on, the number of coloured pixels increased and spreading to the central region, resulting in a circular-like pattern around the corpora hemorrhagica. The spectral waveform was consistent at all timepoints. SPV (cm/s) and EDV (cm/s) presented a numeric trend and a slight statistical difference at all timepoints, whereas no difference was found for RI and PI. CEUS evaluation demonstrated an increase in pixel intensity across all the timepoints. Quantitative CEUS analysis revealed a statistical difference in PPI (%), MTT (s) and AUC (%) at T5. CEUS evaluation of the ovaries was feasible and demonstrated a marked increase in perfusion parameters in the late postovulatory period, demonstrating its applicability in the assessment of canine corpora lutea development. |
publishDate |
2022 |
dc.date.none.fl_str_mv |
2022-12-01 2023-07-29T13:24:25Z 2023-07-29T13:24:25Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1016/j.theriogenology.2022.10.009 Theriogenology, v. 194, p. 162-170. 0093-691X http://hdl.handle.net/11449/247738 10.1016/j.theriogenology.2022.10.009 2-s2.0-85139831028 |
url |
http://dx.doi.org/10.1016/j.theriogenology.2022.10.009 http://hdl.handle.net/11449/247738 |
identifier_str_mv |
Theriogenology, v. 194, p. 162-170. 0093-691X 10.1016/j.theriogenology.2022.10.009 2-s2.0-85139831028 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Theriogenology |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
162-170 |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1799964683350835200 |