Mechanisms Involved in the Beneficial Effects of Spironolactone after Myocardial Infarction
Autor(a) principal: | |
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Data de Publicação: | 2013 |
Outros Autores: | , , , , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1371/journal.pone.0076866 http://hdl.handle.net/11449/76670 |
Resumo: | Introduction:Our objective was to analyze the effect of spironolactone on cardiac remodeling after experimental myocardial infarction (MI), assessed by matricellular proteins levels, cardiac collagen amount and distribution, myocardial tissue metalloproteinase inhibitor-1(TIMP-1) concentration, myocyte hypertrophy, left ventricular architecture, and in vitro and in vivo cardiac function.Methods:Wistar rats were assigned to 4 groups: control group, in which animals were submitted to simulated surgery (SHAM group; n=9); group that received spironolactone and in which animals were submitted to simulated surgery (SHAM-S group, n=9); myocardial infarction group, in which animals were submitted to coronary artery ligation (MI group, n=15); and myocardial infarction group with spironolactone supplementation (MI-S group, n=15). The rats were observed for 3 months.Results:The MI group had higher values of left cardiac chambers and mass index and lower relative wall thicknesses compared with the SHAM group. In addition, diastolic and systolic functions were worse in the MI groups. However, spironolactone did not influence any of these variables. The MI-S group had a lower myocardial hydroxyproline concentration and myocyte cross-sectional area compared with the MI group. Myocardial periostin and collagen type III were lower in the MI-S group compared with the MI-group. In addition, TIMP-1 concentration in myocardium was higher in the MI-S group compared with the MI group.Conclusions:The predominant consequence of spironolactone supplementation after MI is related to reductions in collagens, with discrete attenuation of other remodeling variables. Importantly, this effect may be modulated by periostin and TIMP-1 levels. © 2013 Minicucci et al. |
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Mechanisms Involved in the Beneficial Effects of Spironolactone after Myocardial InfarctionIntroduction:Our objective was to analyze the effect of spironolactone on cardiac remodeling after experimental myocardial infarction (MI), assessed by matricellular proteins levels, cardiac collagen amount and distribution, myocardial tissue metalloproteinase inhibitor-1(TIMP-1) concentration, myocyte hypertrophy, left ventricular architecture, and in vitro and in vivo cardiac function.Methods:Wistar rats were assigned to 4 groups: control group, in which animals were submitted to simulated surgery (SHAM group; n=9); group that received spironolactone and in which animals were submitted to simulated surgery (SHAM-S group, n=9); myocardial infarction group, in which animals were submitted to coronary artery ligation (MI group, n=15); and myocardial infarction group with spironolactone supplementation (MI-S group, n=15). The rats were observed for 3 months.Results:The MI group had higher values of left cardiac chambers and mass index and lower relative wall thicknesses compared with the SHAM group. In addition, diastolic and systolic functions were worse in the MI groups. However, spironolactone did not influence any of these variables. The MI-S group had a lower myocardial hydroxyproline concentration and myocyte cross-sectional area compared with the MI group. Myocardial periostin and collagen type III were lower in the MI-S group compared with the MI-group. In addition, TIMP-1 concentration in myocardium was higher in the MI-S group compared with the MI group.Conclusions:The predominant consequence of spironolactone supplementation after MI is related to reductions in collagens, with discrete attenuation of other remodeling variables. Importantly, this effect may be modulated by periostin and TIMP-1 levels. © 2013 Minicucci et al.Internal Medicine Department Botucatu Medical School University Estadual Paulista, Botucatu, São PauloDepartment of Microbiology and Immunology Institute of Biosciences University Estadual, Paulista, Botucatu, São PauloInternal Medicine Department Botucatu Medical School University Estadual Paulista, Botucatu, São PauloUniversidade Estadual Paulista (Unesp)University EstadualMinicucci, Marcos Ferreira [UNESP]dos Santos, Priscila P. [UNESP]Rafacho, Bruna P. M. [UNESP]Gonçalves, Andrea F. [UNESP]Silva, Renata A. C. [UNESP]Chiuso-Minicucci, FernandaGaiolla, Paula Schmidt Azevedo [UNESP]Polegato, Bertha Furlan [UNESP]Okoshi, Katashi [UNESP]Pereira, Elenize J. [UNESP]Paiva, Sergio Alberto Rupp de [UNESP]Zornoff, Leonardo Antonio Mamede [UNESP]2014-05-27T11:30:45Z2014-05-27T11:30:45Z2013-09-30info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://dx.doi.org/10.1371/journal.pone.0076866PLoS ONE, v. 8, n. 9, 2013.1932-6203http://hdl.handle.net/11449/7667010.1371/journal.pone.0076866WOS:0003254235001792-s2.0-848847320412-s2.0-84884732041.pdf15909715763094205016839015394547121314080140264774387040344716730000-0002-5843-6232Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengPLOS ONE2.7661,164info:eu-repo/semantics/openAccess2023-11-22T06:13:28Zoai:repositorio.unesp.br:11449/76670Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462023-11-22T06:13:28Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Mechanisms Involved in the Beneficial Effects of Spironolactone after Myocardial Infarction |
title |
Mechanisms Involved in the Beneficial Effects of Spironolactone after Myocardial Infarction |
spellingShingle |
Mechanisms Involved in the Beneficial Effects of Spironolactone after Myocardial Infarction Minicucci, Marcos Ferreira [UNESP] |
title_short |
Mechanisms Involved in the Beneficial Effects of Spironolactone after Myocardial Infarction |
title_full |
Mechanisms Involved in the Beneficial Effects of Spironolactone after Myocardial Infarction |
title_fullStr |
Mechanisms Involved in the Beneficial Effects of Spironolactone after Myocardial Infarction |
title_full_unstemmed |
Mechanisms Involved in the Beneficial Effects of Spironolactone after Myocardial Infarction |
title_sort |
Mechanisms Involved in the Beneficial Effects of Spironolactone after Myocardial Infarction |
author |
Minicucci, Marcos Ferreira [UNESP] |
author_facet |
Minicucci, Marcos Ferreira [UNESP] dos Santos, Priscila P. [UNESP] Rafacho, Bruna P. M. [UNESP] Gonçalves, Andrea F. [UNESP] Silva, Renata A. C. [UNESP] Chiuso-Minicucci, Fernanda Gaiolla, Paula Schmidt Azevedo [UNESP] Polegato, Bertha Furlan [UNESP] Okoshi, Katashi [UNESP] Pereira, Elenize J. [UNESP] Paiva, Sergio Alberto Rupp de [UNESP] Zornoff, Leonardo Antonio Mamede [UNESP] |
author_role |
author |
author2 |
dos Santos, Priscila P. [UNESP] Rafacho, Bruna P. M. [UNESP] Gonçalves, Andrea F. [UNESP] Silva, Renata A. C. [UNESP] Chiuso-Minicucci, Fernanda Gaiolla, Paula Schmidt Azevedo [UNESP] Polegato, Bertha Furlan [UNESP] Okoshi, Katashi [UNESP] Pereira, Elenize J. [UNESP] Paiva, Sergio Alberto Rupp de [UNESP] Zornoff, Leonardo Antonio Mamede [UNESP] |
author2_role |
author author author author author author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (Unesp) University Estadual |
dc.contributor.author.fl_str_mv |
Minicucci, Marcos Ferreira [UNESP] dos Santos, Priscila P. [UNESP] Rafacho, Bruna P. M. [UNESP] Gonçalves, Andrea F. [UNESP] Silva, Renata A. C. [UNESP] Chiuso-Minicucci, Fernanda Gaiolla, Paula Schmidt Azevedo [UNESP] Polegato, Bertha Furlan [UNESP] Okoshi, Katashi [UNESP] Pereira, Elenize J. [UNESP] Paiva, Sergio Alberto Rupp de [UNESP] Zornoff, Leonardo Antonio Mamede [UNESP] |
description |
Introduction:Our objective was to analyze the effect of spironolactone on cardiac remodeling after experimental myocardial infarction (MI), assessed by matricellular proteins levels, cardiac collagen amount and distribution, myocardial tissue metalloproteinase inhibitor-1(TIMP-1) concentration, myocyte hypertrophy, left ventricular architecture, and in vitro and in vivo cardiac function.Methods:Wistar rats were assigned to 4 groups: control group, in which animals were submitted to simulated surgery (SHAM group; n=9); group that received spironolactone and in which animals were submitted to simulated surgery (SHAM-S group, n=9); myocardial infarction group, in which animals were submitted to coronary artery ligation (MI group, n=15); and myocardial infarction group with spironolactone supplementation (MI-S group, n=15). The rats were observed for 3 months.Results:The MI group had higher values of left cardiac chambers and mass index and lower relative wall thicknesses compared with the SHAM group. In addition, diastolic and systolic functions were worse in the MI groups. However, spironolactone did not influence any of these variables. The MI-S group had a lower myocardial hydroxyproline concentration and myocyte cross-sectional area compared with the MI group. Myocardial periostin and collagen type III were lower in the MI-S group compared with the MI-group. In addition, TIMP-1 concentration in myocardium was higher in the MI-S group compared with the MI group.Conclusions:The predominant consequence of spironolactone supplementation after MI is related to reductions in collagens, with discrete attenuation of other remodeling variables. Importantly, this effect may be modulated by periostin and TIMP-1 levels. © 2013 Minicucci et al. |
publishDate |
2013 |
dc.date.none.fl_str_mv |
2013-09-30 2014-05-27T11:30:45Z 2014-05-27T11:30:45Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1371/journal.pone.0076866 PLoS ONE, v. 8, n. 9, 2013. 1932-6203 http://hdl.handle.net/11449/76670 10.1371/journal.pone.0076866 WOS:000325423500179 2-s2.0-84884732041 2-s2.0-84884732041.pdf 1590971576309420 5016839015394547 1213140801402647 7438704034471673 0000-0002-5843-6232 |
url |
http://dx.doi.org/10.1371/journal.pone.0076866 http://hdl.handle.net/11449/76670 |
identifier_str_mv |
PLoS ONE, v. 8, n. 9, 2013. 1932-6203 10.1371/journal.pone.0076866 WOS:000325423500179 2-s2.0-84884732041 2-s2.0-84884732041.pdf 1590971576309420 5016839015394547 1213140801402647 7438704034471673 0000-0002-5843-6232 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
PLOS ONE 2.766 1,164 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
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openAccess |
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application/pdf |
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Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
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Repositório Institucional da UNESP |
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Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
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