IRS-1 gene polymorphism and DNA damage in pregnant women with diabetes or mild gestational hyperglycemia
Autor(a) principal: | |
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Data de Publicação: | 2015 |
Outros Autores: | , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://www.dmsjournal.com/content/7/1/30 http://hdl.handle.net/11449/128352 |
Resumo: | Background: Pregnant women with mild gestational hyperglycemia present a high risk for hypertension and obesity, and appear to reproduce the model of metabolic syndrome in pregnancy, including hyperinsulinemia and insulin resistance. Diabetic patients have a higher frequency of the IRS-1 Gly972Arg variant and this polymorphism is directly related to insulin resistance and subsequent hyperglycemia. In diabetes, hyperglycemia and other associated factors generate reactive oxygen species that increase DNA damage. The aims of this study were to evaluate the presence of the IRS-1 Arg972 polymorphism in pregnant women with diabetes or mild gestational hyperglycemia, and in their newborns. Additionally, we evaluated the level of primary DNA damage in lymphocytes of Brazilian pregnant women and the relationship between the amount of genetic damage and presence of the polymorphism.Methods: A based on the oral glucose tolerance test (OGTT) results and on glycemic profiles (GP), as follows: nondiabetic group, mild gestational hyperglycemia (MGH) and diabetic group. Eighty-five newborns were included in the study. Maternal peripheral blood samples and umbilical cord blood samples (5-10 mL) were collected for genotyping by PCR-RFLP and for comet assays.Results: The prevalence of genotype Gly/Arg in pregnant women groups was not statistically significant. In newborns, the frequency of Gly/Arg was significantly higher in the MGH and diabetic groups than in the non-diabetic group. Taken together, groups IIA and IIB (IIA + IIB; diabetes) presented lower amounts of DNA damage than the non-diabetic group (p = 0.064). No significant association was detected between genetic damage and the presence of the Arg972 genotype in pregnant women.Conclusion: The polymorphism was more prevalent in newborns of diabetic and MGH women. We believe that it is necessary to increase the number of subjects to be examined in order to better determine the biological role of the Arg972 polymorphism in these patients. Despite being classified as low-risk, pregnant women with mild gestational hyperglycemia characterize a population of maternal and perinatal adverse outcomes, and that, together with their newborns, require better monitoring by professionals and health services. |
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IRS-1 gene polymorphism and DNA damage in pregnant women with diabetes or mild gestational hyperglycemiaDiabetesMild gestational hyperglycemiaPregnancyNewbornPolymorphismDNA damageBackground: Pregnant women with mild gestational hyperglycemia present a high risk for hypertension and obesity, and appear to reproduce the model of metabolic syndrome in pregnancy, including hyperinsulinemia and insulin resistance. Diabetic patients have a higher frequency of the IRS-1 Gly972Arg variant and this polymorphism is directly related to insulin resistance and subsequent hyperglycemia. In diabetes, hyperglycemia and other associated factors generate reactive oxygen species that increase DNA damage. The aims of this study were to evaluate the presence of the IRS-1 Arg972 polymorphism in pregnant women with diabetes or mild gestational hyperglycemia, and in their newborns. Additionally, we evaluated the level of primary DNA damage in lymphocytes of Brazilian pregnant women and the relationship between the amount of genetic damage and presence of the polymorphism.Methods: A based on the oral glucose tolerance test (OGTT) results and on glycemic profiles (GP), as follows: nondiabetic group, mild gestational hyperglycemia (MGH) and diabetic group. Eighty-five newborns were included in the study. Maternal peripheral blood samples and umbilical cord blood samples (5-10 mL) were collected for genotyping by PCR-RFLP and for comet assays.Results: The prevalence of genotype Gly/Arg in pregnant women groups was not statistically significant. In newborns, the frequency of Gly/Arg was significantly higher in the MGH and diabetic groups than in the non-diabetic group. Taken together, groups IIA and IIB (IIA + IIB; diabetes) presented lower amounts of DNA damage than the non-diabetic group (p = 0.064). No significant association was detected between genetic damage and the presence of the Arg972 genotype in pregnant women.Conclusion: The polymorphism was more prevalent in newborns of diabetic and MGH women. We believe that it is necessary to increase the number of subjects to be examined in order to better determine the biological role of the Arg972 polymorphism in these patients. Despite being classified as low-risk, pregnant women with mild gestational hyperglycemia characterize a population of maternal and perinatal adverse outcomes, and that, together with their newborns, require better monitoring by professionals and health services.Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Universidade Estadual Paulista, Department of Gynecology and Obstetrics, Botucatu Medical School, Unesp_Univ Estadual Paulista, Laboratory of Experimental Research in Gynecology and Obstetrics, Distrito de Rubião Júnior s/n, CEP. 18618.000, Botucatu, São Paulo, BrazilDepartment of Pathology, Laboratory of Toxigenomics and Nutrigenomics, Botucatu Medical School, Unesp_Univ Estadual Paulista, Botucatu, Brazil.FAPESP: 2008/06642-6FAPESP: 2008/06480-6Biomed Central LtdUniversidade Estadual Paulista (Unesp)Gelaleti, Rafael B. [UNESP]Damasceno, Debora C. [UNESP]Salvadori, Daisy M. F. [UNESP]Marcondes, Joao Paulo C. [UNESP]Lima, Paula H. O. [UNESP]Morceli, Glilciane [UNESP]Calderon, Iracema M. P. [UNESP]Rudge, Marilza Vieira Cunha [UNESP]2015-10-21T13:09:12Z2015-10-21T13:09:12Z2015-04-02info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article1-8application/pdfhttp://www.dmsjournal.com/content/7/1/30Diabetology & Metabolic Syndrome. London: Biomed Central Ltd, v. 7, p. 8, 2015.1758-5996http://hdl.handle.net/11449/12835210.1186/s13098-015-0026-3WOS:000352591500001WOS000352591500001.pdf67586803888350780000-0002-9227-832XWeb of Sciencereponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengDiabetology &metabolic Syndrome2.4130,943info:eu-repo/semantics/openAccess2023-11-21T06:13:36Zoai:repositorio.unesp.br:11449/128352Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462023-11-21T06:13:36Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
IRS-1 gene polymorphism and DNA damage in pregnant women with diabetes or mild gestational hyperglycemia |
title |
IRS-1 gene polymorphism and DNA damage in pregnant women with diabetes or mild gestational hyperglycemia |
spellingShingle |
IRS-1 gene polymorphism and DNA damage in pregnant women with diabetes or mild gestational hyperglycemia Gelaleti, Rafael B. [UNESP] Diabetes Mild gestational hyperglycemia Pregnancy Newborn Polymorphism DNA damage |
title_short |
IRS-1 gene polymorphism and DNA damage in pregnant women with diabetes or mild gestational hyperglycemia |
title_full |
IRS-1 gene polymorphism and DNA damage in pregnant women with diabetes or mild gestational hyperglycemia |
title_fullStr |
IRS-1 gene polymorphism and DNA damage in pregnant women with diabetes or mild gestational hyperglycemia |
title_full_unstemmed |
IRS-1 gene polymorphism and DNA damage in pregnant women with diabetes or mild gestational hyperglycemia |
title_sort |
IRS-1 gene polymorphism and DNA damage in pregnant women with diabetes or mild gestational hyperglycemia |
author |
Gelaleti, Rafael B. [UNESP] |
author_facet |
Gelaleti, Rafael B. [UNESP] Damasceno, Debora C. [UNESP] Salvadori, Daisy M. F. [UNESP] Marcondes, Joao Paulo C. [UNESP] Lima, Paula H. O. [UNESP] Morceli, Glilciane [UNESP] Calderon, Iracema M. P. [UNESP] Rudge, Marilza Vieira Cunha [UNESP] |
author_role |
author |
author2 |
Damasceno, Debora C. [UNESP] Salvadori, Daisy M. F. [UNESP] Marcondes, Joao Paulo C. [UNESP] Lima, Paula H. O. [UNESP] Morceli, Glilciane [UNESP] Calderon, Iracema M. P. [UNESP] Rudge, Marilza Vieira Cunha [UNESP] |
author2_role |
author author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (Unesp) |
dc.contributor.author.fl_str_mv |
Gelaleti, Rafael B. [UNESP] Damasceno, Debora C. [UNESP] Salvadori, Daisy M. F. [UNESP] Marcondes, Joao Paulo C. [UNESP] Lima, Paula H. O. [UNESP] Morceli, Glilciane [UNESP] Calderon, Iracema M. P. [UNESP] Rudge, Marilza Vieira Cunha [UNESP] |
dc.subject.por.fl_str_mv |
Diabetes Mild gestational hyperglycemia Pregnancy Newborn Polymorphism DNA damage |
topic |
Diabetes Mild gestational hyperglycemia Pregnancy Newborn Polymorphism DNA damage |
description |
Background: Pregnant women with mild gestational hyperglycemia present a high risk for hypertension and obesity, and appear to reproduce the model of metabolic syndrome in pregnancy, including hyperinsulinemia and insulin resistance. Diabetic patients have a higher frequency of the IRS-1 Gly972Arg variant and this polymorphism is directly related to insulin resistance and subsequent hyperglycemia. In diabetes, hyperglycemia and other associated factors generate reactive oxygen species that increase DNA damage. The aims of this study were to evaluate the presence of the IRS-1 Arg972 polymorphism in pregnant women with diabetes or mild gestational hyperglycemia, and in their newborns. Additionally, we evaluated the level of primary DNA damage in lymphocytes of Brazilian pregnant women and the relationship between the amount of genetic damage and presence of the polymorphism.Methods: A based on the oral glucose tolerance test (OGTT) results and on glycemic profiles (GP), as follows: nondiabetic group, mild gestational hyperglycemia (MGH) and diabetic group. Eighty-five newborns were included in the study. Maternal peripheral blood samples and umbilical cord blood samples (5-10 mL) were collected for genotyping by PCR-RFLP and for comet assays.Results: The prevalence of genotype Gly/Arg in pregnant women groups was not statistically significant. In newborns, the frequency of Gly/Arg was significantly higher in the MGH and diabetic groups than in the non-diabetic group. Taken together, groups IIA and IIB (IIA + IIB; diabetes) presented lower amounts of DNA damage than the non-diabetic group (p = 0.064). No significant association was detected between genetic damage and the presence of the Arg972 genotype in pregnant women.Conclusion: The polymorphism was more prevalent in newborns of diabetic and MGH women. We believe that it is necessary to increase the number of subjects to be examined in order to better determine the biological role of the Arg972 polymorphism in these patients. Despite being classified as low-risk, pregnant women with mild gestational hyperglycemia characterize a population of maternal and perinatal adverse outcomes, and that, together with their newborns, require better monitoring by professionals and health services. |
publishDate |
2015 |
dc.date.none.fl_str_mv |
2015-10-21T13:09:12Z 2015-10-21T13:09:12Z 2015-04-02 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://www.dmsjournal.com/content/7/1/30 Diabetology & Metabolic Syndrome. London: Biomed Central Ltd, v. 7, p. 8, 2015. 1758-5996 http://hdl.handle.net/11449/128352 10.1186/s13098-015-0026-3 WOS:000352591500001 WOS000352591500001.pdf 6758680388835078 0000-0002-9227-832X |
url |
http://www.dmsjournal.com/content/7/1/30 http://hdl.handle.net/11449/128352 |
identifier_str_mv |
Diabetology & Metabolic Syndrome. London: Biomed Central Ltd, v. 7, p. 8, 2015. 1758-5996 10.1186/s13098-015-0026-3 WOS:000352591500001 WOS000352591500001.pdf 6758680388835078 0000-0002-9227-832X |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Diabetology &metabolic Syndrome 2.413 0,943 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
1-8 application/pdf |
dc.publisher.none.fl_str_mv |
Biomed Central Ltd |
publisher.none.fl_str_mv |
Biomed Central Ltd |
dc.source.none.fl_str_mv |
Web of Science reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1799965020322267136 |