Immunodetection of hepatic stellate cells in dogs with visceral leishmaniasis
Autor(a) principal: | |
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Data de Publicação: | 2018 |
Outros Autores: | , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1007/s00436-018-5870-2 http://hdl.handle.net/11449/176233 |
Resumo: | Hepatic stellate cells (HSC), or Ito cells, store vitamin A when at rest but undergo phenotypic changes in situations of liver injury, which may induce fibrosis, and they may participate in the immune response in the liver. The objective of the present study was to investigate the role of HSC in the livers of dogs with visceral leishmaniasis (VL). Twenty-eight livers from dogs infected with VL that were living in an area endemic for the disease were evaluated, among which 13 were asymptomatic (A) and 15 were symptomatic (S). A control group (C) was formed by five dogs from an area that was not endemic for VL. These organs were subjected to histopathological analysis (Masson’s trichrome for fibrosis) and immunohistochemical analysis (Leishmania, smooth-muscle α-actin and TGF-β). In the livers from the symptomatic dogs, a moderate to severe granulomatous inflammatory reaction was observed in the capsule and in the portal, centrilobular and intralobular regions. In the asymptomatic dogs, there was slight to moderate presence of granulomas, and these were even absent in some dogs. The intensity of hepatic fibrosis was predominantly low in the infected dogs (A and S), and fibrosis was absent in the control group. The immunomarking of HSC in the infected groups (A and S) differed significantly (P = 0.0153) from that of the control group. The symptomatic dogs presented the largest number of positive cells. This group also presented a larger number of parasitized macrophages, but did not differ statistically from the asymptomatic group (P > 0.05). The cytokine TGF-β was only detected at low levels, and only in the infected animals, but this did not differ from the control group. Immunomarking for HSC was observed mainly in the nuclei of cells present in the hepatic granulomas of symptomatic dogs and in the sinusoids of the asymptomatic dogs. It was concluded that in the livers of dogs with VL, the HSC are activated and participate in the hepatic response to the parasite. The cytokine TGF-β may be involved in this activation, but in the chronic phase of the infection, this cytokine was detected at lower proportions. It is possible that HSC may also contribute towards chemotaxis of leukocytes for the hepatic compartment, along with other cell types such as Kupffer cells. |
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Immunodetection of hepatic stellate cells in dogs with visceral leishmaniasisHepatic fibrosisIto cellsLeishmania infantumLiverTGF-βHepatic stellate cells (HSC), or Ito cells, store vitamin A when at rest but undergo phenotypic changes in situations of liver injury, which may induce fibrosis, and they may participate in the immune response in the liver. The objective of the present study was to investigate the role of HSC in the livers of dogs with visceral leishmaniasis (VL). Twenty-eight livers from dogs infected with VL that were living in an area endemic for the disease were evaluated, among which 13 were asymptomatic (A) and 15 were symptomatic (S). A control group (C) was formed by five dogs from an area that was not endemic for VL. These organs were subjected to histopathological analysis (Masson’s trichrome for fibrosis) and immunohistochemical analysis (Leishmania, smooth-muscle α-actin and TGF-β). In the livers from the symptomatic dogs, a moderate to severe granulomatous inflammatory reaction was observed in the capsule and in the portal, centrilobular and intralobular regions. In the asymptomatic dogs, there was slight to moderate presence of granulomas, and these were even absent in some dogs. The intensity of hepatic fibrosis was predominantly low in the infected dogs (A and S), and fibrosis was absent in the control group. The immunomarking of HSC in the infected groups (A and S) differed significantly (P = 0.0153) from that of the control group. The symptomatic dogs presented the largest number of positive cells. This group also presented a larger number of parasitized macrophages, but did not differ statistically from the asymptomatic group (P > 0.05). The cytokine TGF-β was only detected at low levels, and only in the infected animals, but this did not differ from the control group. Immunomarking for HSC was observed mainly in the nuclei of cells present in the hepatic granulomas of symptomatic dogs and in the sinusoids of the asymptomatic dogs. It was concluded that in the livers of dogs with VL, the HSC are activated and participate in the hepatic response to the parasite. The cytokine TGF-β may be involved in this activation, but in the chronic phase of the infection, this cytokine was detected at lower proportions. It is possible that HSC may also contribute towards chemotaxis of leukocytes for the hepatic compartment, along with other cell types such as Kupffer cells.Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Departamento de Patologia Veterinária Faculdade de Ciências Agrárias e Veterinárias Universidade Estadual Paulista (UNESP), Via de Acesso Prof. Paulo Donato Castellane, s / nUniversidade do Mississipi Medical CenterDepartamento de Patologia Veterinária Faculdade de Ciências Agrárias e Veterinárias Universidade Estadual Paulista (UNESP), Via de Acesso Prof. Paulo Donato Castellane, s / nFAPESP: 2013/00763-4Universidade Estadual Paulista (Unesp)Universidade do Mississipi Medical CenterMarques, Natália Cassaro [UNESP]Mo reira, Pamela Rodrigues Reina [UNESP]Bertolo, Paulo Henrique Leal [UNESP]Gava, Fábio NelsonVasconcelos, Rosemeri de Oliveira [UNESP]2018-12-11T17:19:43Z2018-12-11T17:19:43Z2018-06-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article1829-1837application/pdfhttp://dx.doi.org/10.1007/s00436-018-5870-2Parasitology Research, v. 117, n. 6, p. 1829-1837, 2018.1432-19550932-0113http://hdl.handle.net/11449/17623310.1007/s00436-018-5870-22-s2.0-850460279802-s2.0-85046027980.pdfScopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengParasitology Research0,9910,991info:eu-repo/semantics/openAccess2024-01-26T06:29:07Zoai:repositorio.unesp.br:11449/176233Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-01-26T06:29:07Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Immunodetection of hepatic stellate cells in dogs with visceral leishmaniasis |
title |
Immunodetection of hepatic stellate cells in dogs with visceral leishmaniasis |
spellingShingle |
Immunodetection of hepatic stellate cells in dogs with visceral leishmaniasis Marques, Natália Cassaro [UNESP] Hepatic fibrosis Ito cells Leishmania infantum Liver TGF-β |
title_short |
Immunodetection of hepatic stellate cells in dogs with visceral leishmaniasis |
title_full |
Immunodetection of hepatic stellate cells in dogs with visceral leishmaniasis |
title_fullStr |
Immunodetection of hepatic stellate cells in dogs with visceral leishmaniasis |
title_full_unstemmed |
Immunodetection of hepatic stellate cells in dogs with visceral leishmaniasis |
title_sort |
Immunodetection of hepatic stellate cells in dogs with visceral leishmaniasis |
author |
Marques, Natália Cassaro [UNESP] |
author_facet |
Marques, Natália Cassaro [UNESP] Mo reira, Pamela Rodrigues Reina [UNESP] Bertolo, Paulo Henrique Leal [UNESP] Gava, Fábio Nelson Vasconcelos, Rosemeri de Oliveira [UNESP] |
author_role |
author |
author2 |
Mo reira, Pamela Rodrigues Reina [UNESP] Bertolo, Paulo Henrique Leal [UNESP] Gava, Fábio Nelson Vasconcelos, Rosemeri de Oliveira [UNESP] |
author2_role |
author author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (Unesp) Universidade do Mississipi Medical Center |
dc.contributor.author.fl_str_mv |
Marques, Natália Cassaro [UNESP] Mo reira, Pamela Rodrigues Reina [UNESP] Bertolo, Paulo Henrique Leal [UNESP] Gava, Fábio Nelson Vasconcelos, Rosemeri de Oliveira [UNESP] |
dc.subject.por.fl_str_mv |
Hepatic fibrosis Ito cells Leishmania infantum Liver TGF-β |
topic |
Hepatic fibrosis Ito cells Leishmania infantum Liver TGF-β |
description |
Hepatic stellate cells (HSC), or Ito cells, store vitamin A when at rest but undergo phenotypic changes in situations of liver injury, which may induce fibrosis, and they may participate in the immune response in the liver. The objective of the present study was to investigate the role of HSC in the livers of dogs with visceral leishmaniasis (VL). Twenty-eight livers from dogs infected with VL that were living in an area endemic for the disease were evaluated, among which 13 were asymptomatic (A) and 15 were symptomatic (S). A control group (C) was formed by five dogs from an area that was not endemic for VL. These organs were subjected to histopathological analysis (Masson’s trichrome for fibrosis) and immunohistochemical analysis (Leishmania, smooth-muscle α-actin and TGF-β). In the livers from the symptomatic dogs, a moderate to severe granulomatous inflammatory reaction was observed in the capsule and in the portal, centrilobular and intralobular regions. In the asymptomatic dogs, there was slight to moderate presence of granulomas, and these were even absent in some dogs. The intensity of hepatic fibrosis was predominantly low in the infected dogs (A and S), and fibrosis was absent in the control group. The immunomarking of HSC in the infected groups (A and S) differed significantly (P = 0.0153) from that of the control group. The symptomatic dogs presented the largest number of positive cells. This group also presented a larger number of parasitized macrophages, but did not differ statistically from the asymptomatic group (P > 0.05). The cytokine TGF-β was only detected at low levels, and only in the infected animals, but this did not differ from the control group. Immunomarking for HSC was observed mainly in the nuclei of cells present in the hepatic granulomas of symptomatic dogs and in the sinusoids of the asymptomatic dogs. It was concluded that in the livers of dogs with VL, the HSC are activated and participate in the hepatic response to the parasite. The cytokine TGF-β may be involved in this activation, but in the chronic phase of the infection, this cytokine was detected at lower proportions. It is possible that HSC may also contribute towards chemotaxis of leukocytes for the hepatic compartment, along with other cell types such as Kupffer cells. |
publishDate |
2018 |
dc.date.none.fl_str_mv |
2018-12-11T17:19:43Z 2018-12-11T17:19:43Z 2018-06-01 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1007/s00436-018-5870-2 Parasitology Research, v. 117, n. 6, p. 1829-1837, 2018. 1432-1955 0932-0113 http://hdl.handle.net/11449/176233 10.1007/s00436-018-5870-2 2-s2.0-85046027980 2-s2.0-85046027980.pdf |
url |
http://dx.doi.org/10.1007/s00436-018-5870-2 http://hdl.handle.net/11449/176233 |
identifier_str_mv |
Parasitology Research, v. 117, n. 6, p. 1829-1837, 2018. 1432-1955 0932-0113 10.1007/s00436-018-5870-2 2-s2.0-85046027980 2-s2.0-85046027980.pdf |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Parasitology Research 0,991 0,991 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
1829-1837 application/pdf |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1792962434936340480 |