Changes in the levels of acute-phase protein and other serum protein fractions in Santa Inês ewes fed with a high-concentrate diet
Autor(a) principal: | |
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Data de Publicação: | 2018 |
Outros Autores: | , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1016/j.smallrumres.2018.03.001 http://hdl.handle.net/11449/175989 |
Resumo: | The aim of this study was to evaluate the effects of subacute ruminal acidosis (SARA) on total serum protein (TP), plasma fibrinogen (Fb), and serum protein fractions in seven hay-adapted Santa Inês ewes fed with a high-concentrate diet for 133 days. Blood was extracted, before feeding, by external jugular venipuncture into tubes with ethylenediaminetetraacetic acid dipotassium anticoagulant, to evaluate the Fb concentration by heat precipitation, and into plain tubes, for TP analysis by the Biuret method and for sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE). SDS-PAGE obtained the following proteins: immunoglobulin A (IgA) and G (IgG); ceruloplasmin (Cp); transferrin (Tf); albumin; α1-antitrypsin (α1AT); haptoglobin (Hp); α1-acid glycoprotein (α1AG); and 140, 101, 95, 46, 36, 34, 31, and 23 kDa molecular weight proteins (MWP). Associations among variables and time were evaluated using regression analysis. Fb concentrations did not change (P > 0.05); IgA, α1AT, and Hp levels increased, while Cp decreased throughout the period. Mean Tf levels increased, reaching the highest value on the 72nd day. The other protein levels oscillated during the study. A positive correlation, measured using Pearson's coefficient, was found between TP and IgG levels (r = 0.77; P < 0.0001). IgA, Hp, Tf, and α1AT showed greater potential for use in the diagnosis of SARA. The occurrence of proteins with unknown functions, but that have been highly influenced by the high-grain diet, increases the possibility of identifying inflammatory markers for SARA. |
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Changes in the levels of acute-phase protein and other serum protein fractions in Santa Inês ewes fed with a high-concentrate dietAcute-phase proteinsInflammationLipopolysaccharidesSerum globulinsSerum protein electrophoresisThe aim of this study was to evaluate the effects of subacute ruminal acidosis (SARA) on total serum protein (TP), plasma fibrinogen (Fb), and serum protein fractions in seven hay-adapted Santa Inês ewes fed with a high-concentrate diet for 133 days. Blood was extracted, before feeding, by external jugular venipuncture into tubes with ethylenediaminetetraacetic acid dipotassium anticoagulant, to evaluate the Fb concentration by heat precipitation, and into plain tubes, for TP analysis by the Biuret method and for sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE). SDS-PAGE obtained the following proteins: immunoglobulin A (IgA) and G (IgG); ceruloplasmin (Cp); transferrin (Tf); albumin; α1-antitrypsin (α1AT); haptoglobin (Hp); α1-acid glycoprotein (α1AG); and 140, 101, 95, 46, 36, 34, 31, and 23 kDa molecular weight proteins (MWP). Associations among variables and time were evaluated using regression analysis. Fb concentrations did not change (P > 0.05); IgA, α1AT, and Hp levels increased, while Cp decreased throughout the period. Mean Tf levels increased, reaching the highest value on the 72nd day. The other protein levels oscillated during the study. A positive correlation, measured using Pearson's coefficient, was found between TP and IgG levels (r = 0.77; P < 0.0001). IgA, Hp, Tf, and α1AT showed greater potential for use in the diagnosis of SARA. The occurrence of proteins with unknown functions, but that have been highly influenced by the high-grain diet, increases the possibility of identifying inflammatory markers for SARA.Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Departamento de Clínica e Cirurgia Veterinária Universidade Estadual Paulista (Unesp) Faculdade de Ciências Agrárias e Veterinárias, Via de Acesso Prof. Paulo Donato Castellane, s/nDepartamento de Ciências Exatas Universidade Estadual Paulista (Unesp) Faculdade de Ciências Agrárias e Veterinárias, Via de Acesso Prof. Paulo Donato Castellane, s/nDepartamento de Clínica e Cirurgia Veterinária Universidade Estadual Paulista (Unesp) Faculdade de Ciências Agrárias e Veterinárias, Via de Acesso Prof. Paulo Donato Castellane, s/nDepartamento de Ciências Exatas Universidade Estadual Paulista (Unesp) Faculdade de Ciências Agrárias e Veterinárias, Via de Acesso Prof. Paulo Donato Castellane, s/nFAPESP: #2012/09111-7FAPESP: #2012/09220-0Universidade Estadual Paulista (Unesp)Girardi, Annita Morais [UNESP]Sabes, Amanda Festa [UNESP]Fagliari, José Jurandir [UNESP]Silva, Paulo César da [UNESP]Oliveira, João Ademir de [UNESP]Marques, Luiz Carlos [UNESP]2018-12-11T17:18:26Z2018-12-11T17:18:26Z2018-05-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article34-38application/pdfhttp://dx.doi.org/10.1016/j.smallrumres.2018.03.001Small Ruminant Research, v. 162, p. 34-38.0921-4488http://hdl.handle.net/11449/17598910.1016/j.smallrumres.2018.03.0012-s2.0-850433418982-s2.0-85043341898.pdf328153847401197992904592614743980000-0001-5898-2914Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengSmall Ruminant Research0,485info:eu-repo/semantics/openAccess2024-01-13T06:29:55Zoai:repositorio.unesp.br:11449/175989Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-01-13T06:29:55Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Changes in the levels of acute-phase protein and other serum protein fractions in Santa Inês ewes fed with a high-concentrate diet |
title |
Changes in the levels of acute-phase protein and other serum protein fractions in Santa Inês ewes fed with a high-concentrate diet |
spellingShingle |
Changes in the levels of acute-phase protein and other serum protein fractions in Santa Inês ewes fed with a high-concentrate diet Girardi, Annita Morais [UNESP] Acute-phase proteins Inflammation Lipopolysaccharides Serum globulins Serum protein electrophoresis |
title_short |
Changes in the levels of acute-phase protein and other serum protein fractions in Santa Inês ewes fed with a high-concentrate diet |
title_full |
Changes in the levels of acute-phase protein and other serum protein fractions in Santa Inês ewes fed with a high-concentrate diet |
title_fullStr |
Changes in the levels of acute-phase protein and other serum protein fractions in Santa Inês ewes fed with a high-concentrate diet |
title_full_unstemmed |
Changes in the levels of acute-phase protein and other serum protein fractions in Santa Inês ewes fed with a high-concentrate diet |
title_sort |
Changes in the levels of acute-phase protein and other serum protein fractions in Santa Inês ewes fed with a high-concentrate diet |
author |
Girardi, Annita Morais [UNESP] |
author_facet |
Girardi, Annita Morais [UNESP] Sabes, Amanda Festa [UNESP] Fagliari, José Jurandir [UNESP] Silva, Paulo César da [UNESP] Oliveira, João Ademir de [UNESP] Marques, Luiz Carlos [UNESP] |
author_role |
author |
author2 |
Sabes, Amanda Festa [UNESP] Fagliari, José Jurandir [UNESP] Silva, Paulo César da [UNESP] Oliveira, João Ademir de [UNESP] Marques, Luiz Carlos [UNESP] |
author2_role |
author author author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (Unesp) |
dc.contributor.author.fl_str_mv |
Girardi, Annita Morais [UNESP] Sabes, Amanda Festa [UNESP] Fagliari, José Jurandir [UNESP] Silva, Paulo César da [UNESP] Oliveira, João Ademir de [UNESP] Marques, Luiz Carlos [UNESP] |
dc.subject.por.fl_str_mv |
Acute-phase proteins Inflammation Lipopolysaccharides Serum globulins Serum protein electrophoresis |
topic |
Acute-phase proteins Inflammation Lipopolysaccharides Serum globulins Serum protein electrophoresis |
description |
The aim of this study was to evaluate the effects of subacute ruminal acidosis (SARA) on total serum protein (TP), plasma fibrinogen (Fb), and serum protein fractions in seven hay-adapted Santa Inês ewes fed with a high-concentrate diet for 133 days. Blood was extracted, before feeding, by external jugular venipuncture into tubes with ethylenediaminetetraacetic acid dipotassium anticoagulant, to evaluate the Fb concentration by heat precipitation, and into plain tubes, for TP analysis by the Biuret method and for sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE). SDS-PAGE obtained the following proteins: immunoglobulin A (IgA) and G (IgG); ceruloplasmin (Cp); transferrin (Tf); albumin; α1-antitrypsin (α1AT); haptoglobin (Hp); α1-acid glycoprotein (α1AG); and 140, 101, 95, 46, 36, 34, 31, and 23 kDa molecular weight proteins (MWP). Associations among variables and time were evaluated using regression analysis. Fb concentrations did not change (P > 0.05); IgA, α1AT, and Hp levels increased, while Cp decreased throughout the period. Mean Tf levels increased, reaching the highest value on the 72nd day. The other protein levels oscillated during the study. A positive correlation, measured using Pearson's coefficient, was found between TP and IgG levels (r = 0.77; P < 0.0001). IgA, Hp, Tf, and α1AT showed greater potential for use in the diagnosis of SARA. The occurrence of proteins with unknown functions, but that have been highly influenced by the high-grain diet, increases the possibility of identifying inflammatory markers for SARA. |
publishDate |
2018 |
dc.date.none.fl_str_mv |
2018-12-11T17:18:26Z 2018-12-11T17:18:26Z 2018-05-01 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1016/j.smallrumres.2018.03.001 Small Ruminant Research, v. 162, p. 34-38. 0921-4488 http://hdl.handle.net/11449/175989 10.1016/j.smallrumres.2018.03.001 2-s2.0-85043341898 2-s2.0-85043341898.pdf 3281538474011979 9290459261474398 0000-0001-5898-2914 |
url |
http://dx.doi.org/10.1016/j.smallrumres.2018.03.001 http://hdl.handle.net/11449/175989 |
identifier_str_mv |
Small Ruminant Research, v. 162, p. 34-38. 0921-4488 10.1016/j.smallrumres.2018.03.001 2-s2.0-85043341898 2-s2.0-85043341898.pdf 3281538474011979 9290459261474398 0000-0001-5898-2914 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Small Ruminant Research 0,485 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
34-38 application/pdf |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
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1799965600887341056 |