Neutrophils, eosinophils, and mast cells in the intestinal wall of dogs naturally infected with Leishmania infantum
Autor(a) principal: | |
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Data de Publicação: | 2018 |
Outros Autores: | , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1590/s1984-296120180085 http://hdl.handle.net/11449/188456 |
Resumo: | Visceral leishmaniasis (VL) is a disease caused by the protozoa Leishmania infantum and can cause an inflammatory reaction in the gastrointestinal tract, however the role of granulocytic cells (neutrophils, eosinophils, and mast cells) in the intestine of dogs infected is not fully understood. We performed a quantitative analysis these cells in the intestinal wall of dogs with canine visceral leishmaniasis (CVL). Twenty dogs were assigned to one of three groups: group 1 (G1, n=8), dogs with CVL and L. infantum amastigotes in the intestine; group 2 (G2, n=9), dogs with CVL but without intestinal amastigotes; and group 3 (G3, n=3), uninfected dogs (control group). Granulocytic cells were counted in the crypt-villus unit (mucosa), submucosa, and muscle layer of the intestinal mucosa. Cell counts were higher in the intestinal wall of dogs from G2 followed by G1 and G3 (p≤0.05). In G1, there was a low inverse correlation between parasite burden of the small intestine and granulocyte counts (r=-0.1, p≤0.01). However, in G2 dogs, mast cell and eosinophil numbers showed positive correlation (r=0.85, p≤0.01). The granulocytic cell hyperplasia observed in the intestine of L. infantum-infected dogs suggests that these cells may be involved in the cell-mediated immune response for parasite elimination. |
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Neutrophils, eosinophils, and mast cells in the intestinal wall of dogs naturally infected with Leishmania infantumNeutrófilos, eosinófilos e mastócitos na parede intestinal de cães naturalmente infectados com Leishmania infantumCanine visceral leishmaniasisGranulocytesIntestinal tractVisceral leishmaniasis (VL) is a disease caused by the protozoa Leishmania infantum and can cause an inflammatory reaction in the gastrointestinal tract, however the role of granulocytic cells (neutrophils, eosinophils, and mast cells) in the intestine of dogs infected is not fully understood. We performed a quantitative analysis these cells in the intestinal wall of dogs with canine visceral leishmaniasis (CVL). Twenty dogs were assigned to one of three groups: group 1 (G1, n=8), dogs with CVL and L. infantum amastigotes in the intestine; group 2 (G2, n=9), dogs with CVL but without intestinal amastigotes; and group 3 (G3, n=3), uninfected dogs (control group). Granulocytic cells were counted in the crypt-villus unit (mucosa), submucosa, and muscle layer of the intestinal mucosa. Cell counts were higher in the intestinal wall of dogs from G2 followed by G1 and G3 (p≤0.05). In G1, there was a low inverse correlation between parasite burden of the small intestine and granulocyte counts (r=-0.1, p≤0.01). However, in G2 dogs, mast cell and eosinophil numbers showed positive correlation (r=0.85, p≤0.01). The granulocytic cell hyperplasia observed in the intestine of L. infantum-infected dogs suggests that these cells may be involved in the cell-mediated immune response for parasite elimination.Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Departamento de Biologia e Ciência Animal Faculdade de Engenharia de Ilha Solteira – FEIS Universidade Estadual Paulista – UNESPUniversidade de São Paulo – USPInstituto de Biotechnologia Universidade Estadual Paulista – UNESPDepartmento de Medicina Veterinária Universidade de São Paulo – USP Faculdade de Engenharia de Alimentos e Ciência Animal – FZEADepartamento de Biologia e Ciência Animal Faculdade de Engenharia de Ilha Solteira – FEIS Universidade Estadual Paulista – UNESPInstituto de Biotechnologia Universidade Estadual Paulista – UNESPFAPESP: 2013/13875-5Universidade Estadual Paulista (Unesp)Universidade de São Paulo (USP)da Silva, Diogo Tiago [UNESP]Alves, Maria Luana [UNESP]Spada, Júlio Cesar Pereira [UNESP]da Silveira, Rita de Cássia Viveiros [UNESP]Oliveira, Trícia Maria Ferreira de SousaStarke-Buzetti, Wilma Aparecida [UNESP]2019-10-06T16:08:36Z2019-10-06T16:08:36Z2018-10-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article430-438application/pdfhttp://dx.doi.org/10.1590/s1984-296120180085Revista Brasileira de Parasitologia Veterinaria, v. 27, n. 4, p. 430-438, 2018.0103-846Xhttp://hdl.handle.net/11449/18845610.1590/s1984-296120180085S1984-296120180004004302-s2.0-85057982549S1984-29612018000400430.pdfScopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengRevista Brasileira de Parasitologia Veterinariainfo:eu-repo/semantics/openAccess2023-10-21T06:05:14Zoai:repositorio.unesp.br:11449/188456Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462023-10-21T06:05:14Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Neutrophils, eosinophils, and mast cells in the intestinal wall of dogs naturally infected with Leishmania infantum Neutrófilos, eosinófilos e mastócitos na parede intestinal de cães naturalmente infectados com Leishmania infantum |
title |
Neutrophils, eosinophils, and mast cells in the intestinal wall of dogs naturally infected with Leishmania infantum |
spellingShingle |
Neutrophils, eosinophils, and mast cells in the intestinal wall of dogs naturally infected with Leishmania infantum da Silva, Diogo Tiago [UNESP] Canine visceral leishmaniasis Granulocytes Intestinal tract |
title_short |
Neutrophils, eosinophils, and mast cells in the intestinal wall of dogs naturally infected with Leishmania infantum |
title_full |
Neutrophils, eosinophils, and mast cells in the intestinal wall of dogs naturally infected with Leishmania infantum |
title_fullStr |
Neutrophils, eosinophils, and mast cells in the intestinal wall of dogs naturally infected with Leishmania infantum |
title_full_unstemmed |
Neutrophils, eosinophils, and mast cells in the intestinal wall of dogs naturally infected with Leishmania infantum |
title_sort |
Neutrophils, eosinophils, and mast cells in the intestinal wall of dogs naturally infected with Leishmania infantum |
author |
da Silva, Diogo Tiago [UNESP] |
author_facet |
da Silva, Diogo Tiago [UNESP] Alves, Maria Luana [UNESP] Spada, Júlio Cesar Pereira [UNESP] da Silveira, Rita de Cássia Viveiros [UNESP] Oliveira, Trícia Maria Ferreira de Sousa Starke-Buzetti, Wilma Aparecida [UNESP] |
author_role |
author |
author2 |
Alves, Maria Luana [UNESP] Spada, Júlio Cesar Pereira [UNESP] da Silveira, Rita de Cássia Viveiros [UNESP] Oliveira, Trícia Maria Ferreira de Sousa Starke-Buzetti, Wilma Aparecida [UNESP] |
author2_role |
author author author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (Unesp) Universidade de São Paulo (USP) |
dc.contributor.author.fl_str_mv |
da Silva, Diogo Tiago [UNESP] Alves, Maria Luana [UNESP] Spada, Júlio Cesar Pereira [UNESP] da Silveira, Rita de Cássia Viveiros [UNESP] Oliveira, Trícia Maria Ferreira de Sousa Starke-Buzetti, Wilma Aparecida [UNESP] |
dc.subject.por.fl_str_mv |
Canine visceral leishmaniasis Granulocytes Intestinal tract |
topic |
Canine visceral leishmaniasis Granulocytes Intestinal tract |
description |
Visceral leishmaniasis (VL) is a disease caused by the protozoa Leishmania infantum and can cause an inflammatory reaction in the gastrointestinal tract, however the role of granulocytic cells (neutrophils, eosinophils, and mast cells) in the intestine of dogs infected is not fully understood. We performed a quantitative analysis these cells in the intestinal wall of dogs with canine visceral leishmaniasis (CVL). Twenty dogs were assigned to one of three groups: group 1 (G1, n=8), dogs with CVL and L. infantum amastigotes in the intestine; group 2 (G2, n=9), dogs with CVL but without intestinal amastigotes; and group 3 (G3, n=3), uninfected dogs (control group). Granulocytic cells were counted in the crypt-villus unit (mucosa), submucosa, and muscle layer of the intestinal mucosa. Cell counts were higher in the intestinal wall of dogs from G2 followed by G1 and G3 (p≤0.05). In G1, there was a low inverse correlation between parasite burden of the small intestine and granulocyte counts (r=-0.1, p≤0.01). However, in G2 dogs, mast cell and eosinophil numbers showed positive correlation (r=0.85, p≤0.01). The granulocytic cell hyperplasia observed in the intestine of L. infantum-infected dogs suggests that these cells may be involved in the cell-mediated immune response for parasite elimination. |
publishDate |
2018 |
dc.date.none.fl_str_mv |
2018-10-01 2019-10-06T16:08:36Z 2019-10-06T16:08:36Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1590/s1984-296120180085 Revista Brasileira de Parasitologia Veterinaria, v. 27, n. 4, p. 430-438, 2018. 0103-846X http://hdl.handle.net/11449/188456 10.1590/s1984-296120180085 S1984-29612018000400430 2-s2.0-85057982549 S1984-29612018000400430.pdf |
url |
http://dx.doi.org/10.1590/s1984-296120180085 http://hdl.handle.net/11449/188456 |
identifier_str_mv |
Revista Brasileira de Parasitologia Veterinaria, v. 27, n. 4, p. 430-438, 2018. 0103-846X 10.1590/s1984-296120180085 S1984-29612018000400430 2-s2.0-85057982549 S1984-29612018000400430.pdf |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Revista Brasileira de Parasitologia Veterinaria |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
430-438 application/pdf |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
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1792961607246020608 |