A preliminary comparison between the effects of red and infrared laser irradiation on viability and proliferation of SHED
Autor(a) principal: | |
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Data de Publicação: | 2019 |
Outros Autores: | , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1007/s10103-018-2615-5 http://hdl.handle.net/11449/185538 |
Resumo: | The aim of this preliminary study was to compare the effects of different energy densities from red and infrared low-level laser (LLL) on viability and proliferation of stem cells from human exfoliated deciduous teeth (SHED). SHED were irradiated with red laser (R) or infrared laser (IR) set with the following dosimetry: 1.2J/cm(2) (0.05J), 2.5J/cm(2) (0.1J), 5.0J/cm(2) (0.2J), and 7.5J/cm(2) (0.3J). Positive (C+) and negative (C-) control groups comprised non-irradiated cells. Data were analyzed by two-way ANOVA followed by Tukey's test (P<0.05). At 24- and 48-h period, group R5.0 showed significantly higher cell viability rates than R1.2 and R2.5. At 48h, R2.5 also revealed lower proliferation than R5.0. Comparing to the C+ group, R2.5 exhibited lower viability at 72h, and proliferation at 24 and 48h. Groups R1.2, IR1.2, and IR5.0 were less viable at 24h, while R1.2, IR2.5, and IR5.0 revealed lower proliferative capacity at 48h. Overall, our results showed that LLL can favor viability and proliferation of SHED, especially when cells receive red laser irradiation at 5.0J/cm(2). Therefore, according to this preliminary investigation, 5J/cm(2) applied by red LLL induced high rates of cell viability and proliferation, while the same irradiation dose using infrared laser led to negative effects. LLL irradiation with 1.2 and 2.5J/cm(2) was deleterious to metabolic activity and proliferation of SHED regardless of the type of laser. Further studies are necessary to gain in-depth knowledge about the effects of different wavelengths of LLL on SHED viability and proliferation. |
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A preliminary comparison between the effects of red and infrared laser irradiation on viability and proliferation of SHEDStem cellsCell proliferationCell survivalLaser therapyLow-level light therapyThe aim of this preliminary study was to compare the effects of different energy densities from red and infrared low-level laser (LLL) on viability and proliferation of stem cells from human exfoliated deciduous teeth (SHED). SHED were irradiated with red laser (R) or infrared laser (IR) set with the following dosimetry: 1.2J/cm(2) (0.05J), 2.5J/cm(2) (0.1J), 5.0J/cm(2) (0.2J), and 7.5J/cm(2) (0.3J). Positive (C+) and negative (C-) control groups comprised non-irradiated cells. Data were analyzed by two-way ANOVA followed by Tukey's test (P<0.05). At 24- and 48-h period, group R5.0 showed significantly higher cell viability rates than R1.2 and R2.5. At 48h, R2.5 also revealed lower proliferation than R5.0. Comparing to the C+ group, R2.5 exhibited lower viability at 72h, and proliferation at 24 and 48h. Groups R1.2, IR1.2, and IR5.0 were less viable at 24h, while R1.2, IR2.5, and IR5.0 revealed lower proliferative capacity at 48h. Overall, our results showed that LLL can favor viability and proliferation of SHED, especially when cells receive red laser irradiation at 5.0J/cm(2). Therefore, according to this preliminary investigation, 5J/cm(2) applied by red LLL induced high rates of cell viability and proliferation, while the same irradiation dose using infrared laser led to negative effects. LLL irradiation with 1.2 and 2.5J/cm(2) was deleterious to metabolic activity and proliferation of SHED regardless of the type of laser. Further studies are necessary to gain in-depth knowledge about the effects of different wavelengths of LLL on SHED viability and proliferation.Fundação de Amparo à Pesquisa do Estado de Minas Gerais (FAPEMIG)Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)Fed Univ Alfenas UNIFAL MG, Sch Dent, Dept Clin & Surg, Rua Gabriel Monteiro da Silva 700, BR-37130000 Alfenas, MG, BrazilJose do Rosario Vellano Univ UNIFENAS, Sch Dent, Dept Pediat Dent, Alfenas, MG, BrazilSao Paulo State Univ, Aracatuba Sch Dent, Dept Restorat Dent Endodont, Sao Paulo, BrazilUniv Sao Paulo, Bauru Sch Dent, Dept Pediat Dent Orthodont & Publ Hlth, Sao Paulo, BrazilSao Paulo State Univ, Aracatuba Sch Dent, Dept Pediat Dent, Sao Paulo, BrazilSao Paulo State Univ, Aracatuba Sch Dent, Dept Restorat Dent Endodont, Sao Paulo, BrazilSao Paulo State Univ, Aracatuba Sch Dent, Dept Pediat Dent, Sao Paulo, BrazilFAPEMIG: APQ-04004-16CAPES: 88881.068437/2014-1SpringerFed Univ Alfenas UNIFAL MGJose do Rosario Vellano Univ UNIFENASUniversidade Estadual Paulista (Unesp)Universidade de São Paulo (USP)Marques, Nelson PereiraLopes, Camila SoaresTeixeira Marques, Nadia CarolinaCosme-Silva, Leopoldo [UNESP]Oliveira, Thais MarchiniDuque, Cristiane [UNESP]Sakai, Vivien ThiemyCosta Hanemann, Joao Adolfo2019-10-04T12:36:21Z2019-10-04T12:36:21Z2019-04-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article465-471http://dx.doi.org/10.1007/s10103-018-2615-5Lasers In Medical Science. London: Springer London Ltd, v. 34, n. 3, p. 465-471, 2019.0268-8921http://hdl.handle.net/11449/18553810.1007/s10103-018-2615-5WOS:00046137150000556518745094936170000-0002-2575-279XWeb of Sciencereponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengLasers In Medical Scienceinfo:eu-repo/semantics/openAccess2022-03-11T11:17:38Zoai:repositorio.unesp.br:11449/185538Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462022-03-11T11:17:38Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
A preliminary comparison between the effects of red and infrared laser irradiation on viability and proliferation of SHED |
title |
A preliminary comparison between the effects of red and infrared laser irradiation on viability and proliferation of SHED |
spellingShingle |
A preliminary comparison between the effects of red and infrared laser irradiation on viability and proliferation of SHED Marques, Nelson Pereira Stem cells Cell proliferation Cell survival Laser therapy Low-level light therapy |
title_short |
A preliminary comparison between the effects of red and infrared laser irradiation on viability and proliferation of SHED |
title_full |
A preliminary comparison between the effects of red and infrared laser irradiation on viability and proliferation of SHED |
title_fullStr |
A preliminary comparison between the effects of red and infrared laser irradiation on viability and proliferation of SHED |
title_full_unstemmed |
A preliminary comparison between the effects of red and infrared laser irradiation on viability and proliferation of SHED |
title_sort |
A preliminary comparison between the effects of red and infrared laser irradiation on viability and proliferation of SHED |
author |
Marques, Nelson Pereira |
author_facet |
Marques, Nelson Pereira Lopes, Camila Soares Teixeira Marques, Nadia Carolina Cosme-Silva, Leopoldo [UNESP] Oliveira, Thais Marchini Duque, Cristiane [UNESP] Sakai, Vivien Thiemy Costa Hanemann, Joao Adolfo |
author_role |
author |
author2 |
Lopes, Camila Soares Teixeira Marques, Nadia Carolina Cosme-Silva, Leopoldo [UNESP] Oliveira, Thais Marchini Duque, Cristiane [UNESP] Sakai, Vivien Thiemy Costa Hanemann, Joao Adolfo |
author2_role |
author author author author author author author |
dc.contributor.none.fl_str_mv |
Fed Univ Alfenas UNIFAL MG Jose do Rosario Vellano Univ UNIFENAS Universidade Estadual Paulista (Unesp) Universidade de São Paulo (USP) |
dc.contributor.author.fl_str_mv |
Marques, Nelson Pereira Lopes, Camila Soares Teixeira Marques, Nadia Carolina Cosme-Silva, Leopoldo [UNESP] Oliveira, Thais Marchini Duque, Cristiane [UNESP] Sakai, Vivien Thiemy Costa Hanemann, Joao Adolfo |
dc.subject.por.fl_str_mv |
Stem cells Cell proliferation Cell survival Laser therapy Low-level light therapy |
topic |
Stem cells Cell proliferation Cell survival Laser therapy Low-level light therapy |
description |
The aim of this preliminary study was to compare the effects of different energy densities from red and infrared low-level laser (LLL) on viability and proliferation of stem cells from human exfoliated deciduous teeth (SHED). SHED were irradiated with red laser (R) or infrared laser (IR) set with the following dosimetry: 1.2J/cm(2) (0.05J), 2.5J/cm(2) (0.1J), 5.0J/cm(2) (0.2J), and 7.5J/cm(2) (0.3J). Positive (C+) and negative (C-) control groups comprised non-irradiated cells. Data were analyzed by two-way ANOVA followed by Tukey's test (P<0.05). At 24- and 48-h period, group R5.0 showed significantly higher cell viability rates than R1.2 and R2.5. At 48h, R2.5 also revealed lower proliferation than R5.0. Comparing to the C+ group, R2.5 exhibited lower viability at 72h, and proliferation at 24 and 48h. Groups R1.2, IR1.2, and IR5.0 were less viable at 24h, while R1.2, IR2.5, and IR5.0 revealed lower proliferative capacity at 48h. Overall, our results showed that LLL can favor viability and proliferation of SHED, especially when cells receive red laser irradiation at 5.0J/cm(2). Therefore, according to this preliminary investigation, 5J/cm(2) applied by red LLL induced high rates of cell viability and proliferation, while the same irradiation dose using infrared laser led to negative effects. LLL irradiation with 1.2 and 2.5J/cm(2) was deleterious to metabolic activity and proliferation of SHED regardless of the type of laser. Further studies are necessary to gain in-depth knowledge about the effects of different wavelengths of LLL on SHED viability and proliferation. |
publishDate |
2019 |
dc.date.none.fl_str_mv |
2019-10-04T12:36:21Z 2019-10-04T12:36:21Z 2019-04-01 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1007/s10103-018-2615-5 Lasers In Medical Science. London: Springer London Ltd, v. 34, n. 3, p. 465-471, 2019. 0268-8921 http://hdl.handle.net/11449/185538 10.1007/s10103-018-2615-5 WOS:000461371500005 5651874509493617 0000-0002-2575-279X |
url |
http://dx.doi.org/10.1007/s10103-018-2615-5 http://hdl.handle.net/11449/185538 |
identifier_str_mv |
Lasers In Medical Science. London: Springer London Ltd, v. 34, n. 3, p. 465-471, 2019. 0268-8921 10.1007/s10103-018-2615-5 WOS:000461371500005 5651874509493617 0000-0002-2575-279X |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Lasers In Medical Science |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
465-471 |
dc.publisher.none.fl_str_mv |
Springer |
publisher.none.fl_str_mv |
Springer |
dc.source.none.fl_str_mv |
Web of Science reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
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1799964582763036672 |