The Postbiotic Activity of Lactobacillus paracasei 28.4 Against Candida auris
Autor(a) principal: | |
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Data de Publicação: | 2020 |
Outros Autores: | , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.3389/fcimb.2020.00397 http://hdl.handle.net/11449/200968 |
Resumo: | Candida auris has emerged as a medically important pathogen with considerable resistance to antifungal agents. The ability to produce biofilms is an important pathogenicity feature of this species that aids escape of host immune responses and antimicrobial agents. The objective of this study was to verify antifungal action using in vitro and in vivo models of the Lactobacillus paracasei 28.4 probiotic cells and postbiotic activity of crude extract (LPCE) and fraction 1 (LPF1), derived from L. paracasei 28.4 supernatant. Both live cells and cells free supernatant of L. paracasei 28.4 inhibited C. auris suggesting probiotic and postbiotic effects. The minimum inhibitory concentration (MIC) for LPCE was 15 mg/mL and ranges from 3.75 to 7.5 mg/mL for LPF1. Killing kinetics determined that after 24 h treatment with LPCE or LPF1 there was a complete reduction of viable C. auris cells compared to fluconazole, which decreased the initial inoculum by 1-logCFU during the same time period. LPCE and LPF1 significantly reduced the biomass (p = 0.0001) and the metabolic activity (p = 0.0001) of C. auris biofilm. There was also a total reduction (~108 CFU/mL) in viability of persister C. auris cells after treatment with postbiotic elements (p < 0.0001). In an in vivo study, injection of LPCE and LPF1 into G. mellonella larvae infected with C. auris prolonged survival of these insects compared to a control group (p < 0.05) and elicited immune responses by increasing the number of circulating hemocytes and gene expression of antimicrobial peptide galiomicin. We concluded that the L. paracasei 28.4 cells and postbiotic elements (LPCE and LPF1) have antifungal activity against planktonic cells, biofilms, and persister cells of C. auris. Postbiotic supplementation derived from L. paracasei 28.4 protected G. mellonella infected with C. auris and enhanced its immune status indicating a dual function in modulating a host immune response. |
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The Postbiotic Activity of Lactobacillus paracasei 28.4 Against Candida aurisbiofilmsCandida aurisLactobacilluspostbioticprobioticCandida auris has emerged as a medically important pathogen with considerable resistance to antifungal agents. The ability to produce biofilms is an important pathogenicity feature of this species that aids escape of host immune responses and antimicrobial agents. The objective of this study was to verify antifungal action using in vitro and in vivo models of the Lactobacillus paracasei 28.4 probiotic cells and postbiotic activity of crude extract (LPCE) and fraction 1 (LPF1), derived from L. paracasei 28.4 supernatant. Both live cells and cells free supernatant of L. paracasei 28.4 inhibited C. auris suggesting probiotic and postbiotic effects. The minimum inhibitory concentration (MIC) for LPCE was 15 mg/mL and ranges from 3.75 to 7.5 mg/mL for LPF1. Killing kinetics determined that after 24 h treatment with LPCE or LPF1 there was a complete reduction of viable C. auris cells compared to fluconazole, which decreased the initial inoculum by 1-logCFU during the same time period. LPCE and LPF1 significantly reduced the biomass (p = 0.0001) and the metabolic activity (p = 0.0001) of C. auris biofilm. There was also a total reduction (~108 CFU/mL) in viability of persister C. auris cells after treatment with postbiotic elements (p < 0.0001). In an in vivo study, injection of LPCE and LPF1 into G. mellonella larvae infected with C. auris prolonged survival of these insects compared to a control group (p < 0.05) and elicited immune responses by increasing the number of circulating hemocytes and gene expression of antimicrobial peptide galiomicin. We concluded that the L. paracasei 28.4 cells and postbiotic elements (LPCE and LPF1) have antifungal activity against planktonic cells, biofilms, and persister cells of C. auris. Postbiotic supplementation derived from L. paracasei 28.4 protected G. mellonella infected with C. auris and enhanced its immune status indicating a dual function in modulating a host immune response.Department of Biosciences and Oral Diagnosis Institute of Science and Technology São Paulo State University/UNESPDivision of Infectious Diseases, Rhode Island Hospital, Warren Alpert Medical School at Brown University, Providence, RI, United StatesDepartment of Organic Chemistry Center for Bioassays Biosynthesis and Ecophysiology of Natural Products Institute of Chemistry São Paulo State University UNESPDepartment of Biosciences and Oral Diagnosis Institute of Science and Technology São Paulo State University/UNESPDepartment of Organic Chemistry Center for Bioassays Biosynthesis and Ecophysiology of Natural Products Institute of Chemistry São Paulo State University UNESPUniversidade Estadual Paulista (Unesp)Rossoni, Rodnei Dennis [UNESP]de Barros, Patrícia Pimentel [UNESP]Mendonça, Iatã do Carmo [UNESP]Medina, Rebeca Previate [UNESP]Silva, Dulce Helena Siqueira [UNESP]Fuchs, Beth BurgwynJunqueira, Juliana Campos [UNESP]Mylonakis, Eleftherios2020-12-12T02:20:44Z2020-12-12T02:20:44Z2020-01-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article397http://dx.doi.org/10.3389/fcimb.2020.00397Frontiers in cellular and infection microbiology, v. 10, p. 397-.2235-2988http://hdl.handle.net/11449/20096810.3389/fcimb.2020.003972-s2.0-85089975477Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengFrontiers in cellular and infection microbiologyinfo:eu-repo/semantics/openAccess2021-10-23T15:41:28Zoai:repositorio.unesp.br:11449/200968Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462021-10-23T15:41:28Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
The Postbiotic Activity of Lactobacillus paracasei 28.4 Against Candida auris |
title |
The Postbiotic Activity of Lactobacillus paracasei 28.4 Against Candida auris |
spellingShingle |
The Postbiotic Activity of Lactobacillus paracasei 28.4 Against Candida auris Rossoni, Rodnei Dennis [UNESP] biofilms Candida auris Lactobacillus postbiotic probiotic |
title_short |
The Postbiotic Activity of Lactobacillus paracasei 28.4 Against Candida auris |
title_full |
The Postbiotic Activity of Lactobacillus paracasei 28.4 Against Candida auris |
title_fullStr |
The Postbiotic Activity of Lactobacillus paracasei 28.4 Against Candida auris |
title_full_unstemmed |
The Postbiotic Activity of Lactobacillus paracasei 28.4 Against Candida auris |
title_sort |
The Postbiotic Activity of Lactobacillus paracasei 28.4 Against Candida auris |
author |
Rossoni, Rodnei Dennis [UNESP] |
author_facet |
Rossoni, Rodnei Dennis [UNESP] de Barros, Patrícia Pimentel [UNESP] Mendonça, Iatã do Carmo [UNESP] Medina, Rebeca Previate [UNESP] Silva, Dulce Helena Siqueira [UNESP] Fuchs, Beth Burgwyn Junqueira, Juliana Campos [UNESP] Mylonakis, Eleftherios |
author_role |
author |
author2 |
de Barros, Patrícia Pimentel [UNESP] Mendonça, Iatã do Carmo [UNESP] Medina, Rebeca Previate [UNESP] Silva, Dulce Helena Siqueira [UNESP] Fuchs, Beth Burgwyn Junqueira, Juliana Campos [UNESP] Mylonakis, Eleftherios |
author2_role |
author author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (Unesp) |
dc.contributor.author.fl_str_mv |
Rossoni, Rodnei Dennis [UNESP] de Barros, Patrícia Pimentel [UNESP] Mendonça, Iatã do Carmo [UNESP] Medina, Rebeca Previate [UNESP] Silva, Dulce Helena Siqueira [UNESP] Fuchs, Beth Burgwyn Junqueira, Juliana Campos [UNESP] Mylonakis, Eleftherios |
dc.subject.por.fl_str_mv |
biofilms Candida auris Lactobacillus postbiotic probiotic |
topic |
biofilms Candida auris Lactobacillus postbiotic probiotic |
description |
Candida auris has emerged as a medically important pathogen with considerable resistance to antifungal agents. The ability to produce biofilms is an important pathogenicity feature of this species that aids escape of host immune responses and antimicrobial agents. The objective of this study was to verify antifungal action using in vitro and in vivo models of the Lactobacillus paracasei 28.4 probiotic cells and postbiotic activity of crude extract (LPCE) and fraction 1 (LPF1), derived from L. paracasei 28.4 supernatant. Both live cells and cells free supernatant of L. paracasei 28.4 inhibited C. auris suggesting probiotic and postbiotic effects. The minimum inhibitory concentration (MIC) for LPCE was 15 mg/mL and ranges from 3.75 to 7.5 mg/mL for LPF1. Killing kinetics determined that after 24 h treatment with LPCE or LPF1 there was a complete reduction of viable C. auris cells compared to fluconazole, which decreased the initial inoculum by 1-logCFU during the same time period. LPCE and LPF1 significantly reduced the biomass (p = 0.0001) and the metabolic activity (p = 0.0001) of C. auris biofilm. There was also a total reduction (~108 CFU/mL) in viability of persister C. auris cells after treatment with postbiotic elements (p < 0.0001). In an in vivo study, injection of LPCE and LPF1 into G. mellonella larvae infected with C. auris prolonged survival of these insects compared to a control group (p < 0.05) and elicited immune responses by increasing the number of circulating hemocytes and gene expression of antimicrobial peptide galiomicin. We concluded that the L. paracasei 28.4 cells and postbiotic elements (LPCE and LPF1) have antifungal activity against planktonic cells, biofilms, and persister cells of C. auris. Postbiotic supplementation derived from L. paracasei 28.4 protected G. mellonella infected with C. auris and enhanced its immune status indicating a dual function in modulating a host immune response. |
publishDate |
2020 |
dc.date.none.fl_str_mv |
2020-12-12T02:20:44Z 2020-12-12T02:20:44Z 2020-01-01 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.3389/fcimb.2020.00397 Frontiers in cellular and infection microbiology, v. 10, p. 397-. 2235-2988 http://hdl.handle.net/11449/200968 10.3389/fcimb.2020.00397 2-s2.0-85089975477 |
url |
http://dx.doi.org/10.3389/fcimb.2020.00397 http://hdl.handle.net/11449/200968 |
identifier_str_mv |
Frontiers in cellular and infection microbiology, v. 10, p. 397-. 2235-2988 10.3389/fcimb.2020.00397 2-s2.0-85089975477 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Frontiers in cellular and infection microbiology |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
397 |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
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1799964605887283200 |