Diversidade genética de quatro linhagens de Oreochromis niloticus utilizando o marcador RAPD

Detalhes bibliográficos
Autor(a) principal: Massago, Haluko [UNESP]
Data de Publicação: 2009
Outros Autores: Ribeiro, Ricardo Pereira, Lopera Barrero, Nelson Mauricio, Povh, Jayme Aparecido, Castagnolli, Newton, Gomes, Patricia Cristina
Tipo de documento: Artigo
Idioma: por
Título da fonte: Repositório Institucional da UNESP
Texto Completo: http://www.seer.ufu.br/index.php/biosciencejournal/article/viewFile/6949/4605
http://hdl.handle.net/11449/40081
Resumo: The aim of this study was to analyze the genetic diversity of four Nile Tilapia (Oreochromis niloticus) strains using the RAPD marker. Fin samples of GIFT (G), Chitralada (C), Supreme (S) and Bouake (B) juvenile stocks have been collected. The 11 primers used yielded 81 fragments of which 41.98% were polymorphic. The percentage of polymorphic loci (G: 18.52%; C: 19.75%; S: 20.99% and B: 24.79%) showed that there was a genetic differentiation among the strains, showing the G(st) values a high (BxG: 0.231; BxC: 0.224; GxC: 0.194 and SxC: 0.208) and elevated (BxS: 0.315 and GxS: 0.270) differentiation. The highest gene flow (N(m)) was among the GxC (2.082) strains. The distance and genetic identity values (0.044 and 0.957 respectively) and the dendrogram indicate that the GxC is the most genetically similar strains. The genetic similarity was high among of the strains (G: 0,932; C: 0,903; S: 0,891 and B: 0.900). These results will enable a correct reproductive and genetic strains management.
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spelling Diversidade genética de quatro linhagens de Oreochromis niloticus utilizando o marcador RAPDGenetic diversity of four Oreochromis niloticus strains using the rapd markerBouakeChitraladaGenetic variabilityGIFTSupremeTilapiaThe aim of this study was to analyze the genetic diversity of four Nile Tilapia (Oreochromis niloticus) strains using the RAPD marker. Fin samples of GIFT (G), Chitralada (C), Supreme (S) and Bouake (B) juvenile stocks have been collected. The 11 primers used yielded 81 fragments of which 41.98% were polymorphic. The percentage of polymorphic loci (G: 18.52%; C: 19.75%; S: 20.99% and B: 24.79%) showed that there was a genetic differentiation among the strains, showing the G(st) values a high (BxG: 0.231; BxC: 0.224; GxC: 0.194 and SxC: 0.208) and elevated (BxS: 0.315 and GxS: 0.270) differentiation. The highest gene flow (N(m)) was among the GxC (2.082) strains. The distance and genetic identity values (0.044 and 0.957 respectively) and the dendrogram indicate that the GxC is the most genetically similar strains. The genetic similarity was high among of the strains (G: 0,932; C: 0,903; S: 0,891 and B: 0.900). These results will enable a correct reproductive and genetic strains management.O objetivo do presente estudo foi analisar a diversidade genética de quatro linhagens de tilápia do Nilo (Oreochromis niloticus) utilizando o marcador RAPD. Foram coletadas amostras de nadadeira de estoques de juvenis das linhagens GIFT (G), Chitralada (C), Supreme (S) e Bouaké (B). Os 11 primers utilizados produziram 81 fragmentos dos quais 41,98% foram polimórficos. A porcentagem de fragmentos polimórficos (G: 18,52%; C: 19,75%; S: 20,99% e B: 24,79%) confirmaram que houve uma diferenciação genética entre as linhagens, mostrando os valores de Gst uma alta (BxG: 0,231; BxC: 0,224; GxC: 0,194 e SxC: 0,208) e elevada (BxS: 0,315 e GxS: 0,270) diferenciação. O fluxo gênico (Nm) foi maior entre as linhagens GxC (2,082). Os valores de distância e identidade genética (0,044 e 0,957 respectivamente) e o dendrograma indicam que as linhagens GxC são os mais semelhantes geneticamente. A similaridade genética foi alta dentro das linhagens (G: 0,932; C: 0,903; S: 0,891 e B: 0.900). Os resultados deste estudo possibilitarão o correto manejo reprodutivo e genético das linhagens.UNESP, CA, Jaboticabal, SP, BrazilUniversidade Estadual de Maringá (UEM), CCA, Programa Posgrad Zootecnia, Maringa, Parana, BrazilUniv Tolima, Fac Biol, Dept Ciencias, Ibague Tolima, ColombiaUniv Fed Mato Grosso, Dept Zootecnia, Cuiaba, MT USACastagnolli Consultoria SS Ltda, Jaboticabal, SP, BrazilUNESP, CA, Jaboticabal, SP, BrazilUniversidade Federal de Uberlândia (UFU)Universidade Estadual Paulista (Unesp)Universidade Estadual de Maringá (UEM)Univ TolimaUniv Fed Mato GrossoCastagnolli Consultoria SS LtdaMassago, Haluko [UNESP]Ribeiro, Ricardo PereiraLopera Barrero, Nelson MauricioPovh, Jayme AparecidoCastagnolli, NewtonGomes, Patricia Cristina2014-05-20T15:30:46Z2014-05-20T15:30:46Z2009-01-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article142-151http://www.seer.ufu.br/index.php/biosciencejournal/article/viewFile/6949/4605Bioscience Journal. Uberlandia: Universidade Federal de Uberlândia (UFU), v. 25, n. 4, p. 142-151, 2009.1516-3725http://hdl.handle.net/11449/40081WOS:000269317400018Web of Sciencereponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPporBioscience Journal0,303info:eu-repo/semantics/openAccess2021-10-23T10:41:03Zoai:repositorio.unesp.br:11449/40081Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462021-10-23T10:41:03Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false
dc.title.none.fl_str_mv Diversidade genética de quatro linhagens de Oreochromis niloticus utilizando o marcador RAPD
Genetic diversity of four Oreochromis niloticus strains using the rapd marker
title Diversidade genética de quatro linhagens de Oreochromis niloticus utilizando o marcador RAPD
spellingShingle Diversidade genética de quatro linhagens de Oreochromis niloticus utilizando o marcador RAPD
Massago, Haluko [UNESP]
Bouake
Chitralada
Genetic variability
GIFT
Supreme
Tilapia
title_short Diversidade genética de quatro linhagens de Oreochromis niloticus utilizando o marcador RAPD
title_full Diversidade genética de quatro linhagens de Oreochromis niloticus utilizando o marcador RAPD
title_fullStr Diversidade genética de quatro linhagens de Oreochromis niloticus utilizando o marcador RAPD
title_full_unstemmed Diversidade genética de quatro linhagens de Oreochromis niloticus utilizando o marcador RAPD
title_sort Diversidade genética de quatro linhagens de Oreochromis niloticus utilizando o marcador RAPD
author Massago, Haluko [UNESP]
author_facet Massago, Haluko [UNESP]
Ribeiro, Ricardo Pereira
Lopera Barrero, Nelson Mauricio
Povh, Jayme Aparecido
Castagnolli, Newton
Gomes, Patricia Cristina
author_role author
author2 Ribeiro, Ricardo Pereira
Lopera Barrero, Nelson Mauricio
Povh, Jayme Aparecido
Castagnolli, Newton
Gomes, Patricia Cristina
author2_role author
author
author
author
author
dc.contributor.none.fl_str_mv Universidade Estadual Paulista (Unesp)
Universidade Estadual de Maringá (UEM)
Univ Tolima
Univ Fed Mato Grosso
Castagnolli Consultoria SS Ltda
dc.contributor.author.fl_str_mv Massago, Haluko [UNESP]
Ribeiro, Ricardo Pereira
Lopera Barrero, Nelson Mauricio
Povh, Jayme Aparecido
Castagnolli, Newton
Gomes, Patricia Cristina
dc.subject.por.fl_str_mv Bouake
Chitralada
Genetic variability
GIFT
Supreme
Tilapia
topic Bouake
Chitralada
Genetic variability
GIFT
Supreme
Tilapia
description The aim of this study was to analyze the genetic diversity of four Nile Tilapia (Oreochromis niloticus) strains using the RAPD marker. Fin samples of GIFT (G), Chitralada (C), Supreme (S) and Bouake (B) juvenile stocks have been collected. The 11 primers used yielded 81 fragments of which 41.98% were polymorphic. The percentage of polymorphic loci (G: 18.52%; C: 19.75%; S: 20.99% and B: 24.79%) showed that there was a genetic differentiation among the strains, showing the G(st) values a high (BxG: 0.231; BxC: 0.224; GxC: 0.194 and SxC: 0.208) and elevated (BxS: 0.315 and GxS: 0.270) differentiation. The highest gene flow (N(m)) was among the GxC (2.082) strains. The distance and genetic identity values (0.044 and 0.957 respectively) and the dendrogram indicate that the GxC is the most genetically similar strains. The genetic similarity was high among of the strains (G: 0,932; C: 0,903; S: 0,891 and B: 0.900). These results will enable a correct reproductive and genetic strains management.
publishDate 2009
dc.date.none.fl_str_mv 2009-01-01
2014-05-20T15:30:46Z
2014-05-20T15:30:46Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://www.seer.ufu.br/index.php/biosciencejournal/article/viewFile/6949/4605
Bioscience Journal. Uberlandia: Universidade Federal de Uberlândia (UFU), v. 25, n. 4, p. 142-151, 2009.
1516-3725
http://hdl.handle.net/11449/40081
WOS:000269317400018
url http://www.seer.ufu.br/index.php/biosciencejournal/article/viewFile/6949/4605
http://hdl.handle.net/11449/40081
identifier_str_mv Bioscience Journal. Uberlandia: Universidade Federal de Uberlândia (UFU), v. 25, n. 4, p. 142-151, 2009.
1516-3725
WOS:000269317400018
dc.language.iso.fl_str_mv por
language por
dc.relation.none.fl_str_mv Bioscience Journal
0,303
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 142-151
dc.publisher.none.fl_str_mv Universidade Federal de Uberlândia (UFU)
publisher.none.fl_str_mv Universidade Federal de Uberlândia (UFU)
dc.source.none.fl_str_mv Web of Science
reponame:Repositório Institucional da UNESP
instname:Universidade Estadual Paulista (UNESP)
instacron:UNESP
instname_str Universidade Estadual Paulista (UNESP)
instacron_str UNESP
institution UNESP
reponame_str Repositório Institucional da UNESP
collection Repositório Institucional da UNESP
repository.name.fl_str_mv Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)
repository.mail.fl_str_mv
_version_ 1792961489172168704

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