Evidence for sub-functionalization of tandemly duplicated XPB nucleotide excision repair genes in Arabidopsis thaliana
Autor(a) principal: | |
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Data de Publicação: | 2020 |
Outros Autores: | , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1016/j.gene.2020.144818 http://hdl.handle.net/11449/200567 |
Resumo: | Plants are continuously exposed to agents that can generate DNA lesions. Nucleotide Excision Repair (NER) is one of the repair pathways employed by plants to protect their genome, including from sunlight. The Xeroderma Pigmentosum type B (XPB) protein is a DNA helicase shown to be involved in NER and is also an essential subunit of the Transcription Factor IIH (TFIIH) complex. XPB was found to be a single copy gene in eukaryotes, but found as a tandem duplication in the plant Arabidopsis thaliana, AtXPB1 and AtXPB2. We aimed to investigate whether the XPB in tandem duplication was common within members of the Brassicaceae. We analyzed genomic DNA of species from different tribes of the family and the results indicate that the tandem duplication occurred in Camelineae tribe ancestor, of which A. thaliana belongs, at approximately 8 million years ago. Further experiments were devised to study possible functional roles for the A. thaliana AtXPB paralogs. A non-coincident expression profile of the paralogs was observed in various plant organs, developmental and cell cycle stages. AtXPB2 expression was observed in proliferating cells and clustered with the transcription of other components of the TFIIH such as p44, p52 and XPD/UVH6 along the cell cycle. AtXPB1 gene transcription, on the other hand, was enhanced specifically after UV-B irradiation in leaf trichomes. Altogether, our results reported herein suggest a functional specialization for the AtXPB paralogs: while the AtXPB2 paralog may have a role in cell proliferation and repair as XPB of other eukaryotes, the AtXPB1 paralog is most likely implicated in repair functions in highly specialized A. thaliana cells. |
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Evidence for sub-functionalization of tandemly duplicated XPB nucleotide excision repair genes in Arabidopsis thalianaCell cycleExpression profileFunctional specializationGene duplicationNERUV irradiationXPBPlants are continuously exposed to agents that can generate DNA lesions. Nucleotide Excision Repair (NER) is one of the repair pathways employed by plants to protect their genome, including from sunlight. The Xeroderma Pigmentosum type B (XPB) protein is a DNA helicase shown to be involved in NER and is also an essential subunit of the Transcription Factor IIH (TFIIH) complex. XPB was found to be a single copy gene in eukaryotes, but found as a tandem duplication in the plant Arabidopsis thaliana, AtXPB1 and AtXPB2. We aimed to investigate whether the XPB in tandem duplication was common within members of the Brassicaceae. We analyzed genomic DNA of species from different tribes of the family and the results indicate that the tandem duplication occurred in Camelineae tribe ancestor, of which A. thaliana belongs, at approximately 8 million years ago. Further experiments were devised to study possible functional roles for the A. thaliana AtXPB paralogs. A non-coincident expression profile of the paralogs was observed in various plant organs, developmental and cell cycle stages. AtXPB2 expression was observed in proliferating cells and clustered with the transcription of other components of the TFIIH such as p44, p52 and XPD/UVH6 along the cell cycle. AtXPB1 gene transcription, on the other hand, was enhanced specifically after UV-B irradiation in leaf trichomes. Altogether, our results reported herein suggest a functional specialization for the AtXPB paralogs: while the AtXPB2 paralog may have a role in cell proliferation and repair as XPB of other eukaryotes, the AtXPB1 paralog is most likely implicated in repair functions in highly specialized A. thaliana cells.Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)Departamento de Botânica Instituto de Biociências Universidade de São PauloCentro de Ciências Naturais e Humanas Universidade Federal do ABC (UFABC) São Bernardo do CampoDepartamento de Microbiologia Instituto de Ciências Biomédicas Universidade de São PauloUniversidade Estadual Paulista (UNESP), Campus de Registro São PauloLaboratório de Biologia Vascular Instituto do Coração (InCor) da Faculdade de Medicina Universidade de São PauloUniversidade Estadual Paulista (UNESP), Campus de Registro São PauloFAPESP: 1998/03925-3FAPESP: 2009/52417-7FAPESP: 2014/15982-6CNPq: 310779/2017-0Universidade de São Paulo (USP)Universidade Federal do ABC (UFABC)Universidade Estadual Paulista (Unesp)Masuda, Hana PaulaNakabashi, MynaMorgante, Patricia G [UNESP]Kajihara, Danielade Setta, NathaliaMenck, Carlos Frederico MartinsVan Sluys, Marie-Anne2020-12-12T02:09:59Z2020-12-12T02:09:59Z2020-09-05info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlehttp://dx.doi.org/10.1016/j.gene.2020.144818Gene, v. 754.1879-00380378-1119http://hdl.handle.net/11449/20056710.1016/j.gene.2020.1448182-s2.0-85085965390Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengGeneinfo:eu-repo/semantics/openAccess2024-05-03T13:20:22Zoai:repositorio.unesp.br:11449/200567Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-05-03T13:20:22Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Evidence for sub-functionalization of tandemly duplicated XPB nucleotide excision repair genes in Arabidopsis thaliana |
title |
Evidence for sub-functionalization of tandemly duplicated XPB nucleotide excision repair genes in Arabidopsis thaliana |
spellingShingle |
Evidence for sub-functionalization of tandemly duplicated XPB nucleotide excision repair genes in Arabidopsis thaliana Masuda, Hana Paula Cell cycle Expression profile Functional specialization Gene duplication NER UV irradiation XPB |
title_short |
Evidence for sub-functionalization of tandemly duplicated XPB nucleotide excision repair genes in Arabidopsis thaliana |
title_full |
Evidence for sub-functionalization of tandemly duplicated XPB nucleotide excision repair genes in Arabidopsis thaliana |
title_fullStr |
Evidence for sub-functionalization of tandemly duplicated XPB nucleotide excision repair genes in Arabidopsis thaliana |
title_full_unstemmed |
Evidence for sub-functionalization of tandemly duplicated XPB nucleotide excision repair genes in Arabidopsis thaliana |
title_sort |
Evidence for sub-functionalization of tandemly duplicated XPB nucleotide excision repair genes in Arabidopsis thaliana |
author |
Masuda, Hana Paula |
author_facet |
Masuda, Hana Paula Nakabashi, Myna Morgante, Patricia G [UNESP] Kajihara, Daniela de Setta, Nathalia Menck, Carlos Frederico Martins Van Sluys, Marie-Anne |
author_role |
author |
author2 |
Nakabashi, Myna Morgante, Patricia G [UNESP] Kajihara, Daniela de Setta, Nathalia Menck, Carlos Frederico Martins Van Sluys, Marie-Anne |
author2_role |
author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade de São Paulo (USP) Universidade Federal do ABC (UFABC) Universidade Estadual Paulista (Unesp) |
dc.contributor.author.fl_str_mv |
Masuda, Hana Paula Nakabashi, Myna Morgante, Patricia G [UNESP] Kajihara, Daniela de Setta, Nathalia Menck, Carlos Frederico Martins Van Sluys, Marie-Anne |
dc.subject.por.fl_str_mv |
Cell cycle Expression profile Functional specialization Gene duplication NER UV irradiation XPB |
topic |
Cell cycle Expression profile Functional specialization Gene duplication NER UV irradiation XPB |
description |
Plants are continuously exposed to agents that can generate DNA lesions. Nucleotide Excision Repair (NER) is one of the repair pathways employed by plants to protect their genome, including from sunlight. The Xeroderma Pigmentosum type B (XPB) protein is a DNA helicase shown to be involved in NER and is also an essential subunit of the Transcription Factor IIH (TFIIH) complex. XPB was found to be a single copy gene in eukaryotes, but found as a tandem duplication in the plant Arabidopsis thaliana, AtXPB1 and AtXPB2. We aimed to investigate whether the XPB in tandem duplication was common within members of the Brassicaceae. We analyzed genomic DNA of species from different tribes of the family and the results indicate that the tandem duplication occurred in Camelineae tribe ancestor, of which A. thaliana belongs, at approximately 8 million years ago. Further experiments were devised to study possible functional roles for the A. thaliana AtXPB paralogs. A non-coincident expression profile of the paralogs was observed in various plant organs, developmental and cell cycle stages. AtXPB2 expression was observed in proliferating cells and clustered with the transcription of other components of the TFIIH such as p44, p52 and XPD/UVH6 along the cell cycle. AtXPB1 gene transcription, on the other hand, was enhanced specifically after UV-B irradiation in leaf trichomes. Altogether, our results reported herein suggest a functional specialization for the AtXPB paralogs: while the AtXPB2 paralog may have a role in cell proliferation and repair as XPB of other eukaryotes, the AtXPB1 paralog is most likely implicated in repair functions in highly specialized A. thaliana cells. |
publishDate |
2020 |
dc.date.none.fl_str_mv |
2020-12-12T02:09:59Z 2020-12-12T02:09:59Z 2020-09-05 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1016/j.gene.2020.144818 Gene, v. 754. 1879-0038 0378-1119 http://hdl.handle.net/11449/200567 10.1016/j.gene.2020.144818 2-s2.0-85085965390 |
url |
http://dx.doi.org/10.1016/j.gene.2020.144818 http://hdl.handle.net/11449/200567 |
identifier_str_mv |
Gene, v. 754. 1879-0038 0378-1119 10.1016/j.gene.2020.144818 2-s2.0-85085965390 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Gene |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
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1799965552549036032 |