Characterization of phytoplankton by pigment analysis and the detection of toxic cyanobacteria in reservoirs with aquaculture production
Autor(a) principal: | |
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Data de Publicação: | 2018 |
Outros Autores: | , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.3354/AEI00256 http://hdl.handle.net/11449/176285 |
Resumo: | The intensified use of water bodies and reservoirs for aquaculture production has increased the need for monitoring and early warning of toxins from cyanobacteria. To minimize effects from toxins, simple and fast analytical monitoring methods are crucial. Here, the content of pigments and microcystins in 14 different strains of cyanobacteria cultured under different growth conditions was investigated to determine the influence of light and nutrient starvation on pigment/ chlorophyll a (chl a) ratios. The obtained pigment/chl a ratios were applied in the software CHEMTAX to calculate the biomass of toxic cyanobacteria, as well as other phytoplankton groups. CHEMTAX ratios from the laboratory cultures were applied to water samples collected during 4 sampling periods at 6 fish farms in different reservoirs in São Paulo State, Brazil. Cyanobacteria generally dominated in all reservoirs in all sampling periods and constituted on average 44 to 66% of the average phytoplankton biomass. The concentrations of microcystins were significantly correlated with the chl a concentrations of cyanobacteria and showed that the pigment method can be used to detect microcystin-producing cyanobacteria in these Brazilian reservoirs. When the concentration of cyanobacteria in the reservoirs was above 4 μg chl a l-1, microcystins were always detected. Our results show that pigment analysis can be used to provide fast and reliable results for the early warning, the presence and potential risk of toxic cyanobacteria in freshwater reservoirs used for aqua culture. |
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Characterization of phytoplankton by pigment analysis and the detection of toxic cyanobacteria in reservoirs with aquaculture productionCyanobacteriaFish farmHPLCMicrocystinsPhytoplankton compositionPigment analysisThe intensified use of water bodies and reservoirs for aquaculture production has increased the need for monitoring and early warning of toxins from cyanobacteria. To minimize effects from toxins, simple and fast analytical monitoring methods are crucial. Here, the content of pigments and microcystins in 14 different strains of cyanobacteria cultured under different growth conditions was investigated to determine the influence of light and nutrient starvation on pigment/ chlorophyll a (chl a) ratios. The obtained pigment/chl a ratios were applied in the software CHEMTAX to calculate the biomass of toxic cyanobacteria, as well as other phytoplankton groups. CHEMTAX ratios from the laboratory cultures were applied to water samples collected during 4 sampling periods at 6 fish farms in different reservoirs in São Paulo State, Brazil. Cyanobacteria generally dominated in all reservoirs in all sampling periods and constituted on average 44 to 66% of the average phytoplankton biomass. The concentrations of microcystins were significantly correlated with the chl a concentrations of cyanobacteria and showed that the pigment method can be used to detect microcystin-producing cyanobacteria in these Brazilian reservoirs. When the concentration of cyanobacteria in the reservoirs was above 4 μg chl a l-1, microcystins were always detected. Our results show that pigment analysis can be used to provide fast and reliable results for the early warning, the presence and potential risk of toxic cyanobacteria in freshwater reservoirs used for aqua culture.Environment and Toxicology DHISão Paulo State Agribusiness Agency TechnologySection of Microbial Ecology and Biotechnology Department of Plant and Environmental Sciences University of CopenhagenFicology Department Botanical InstituteUNESP Campus Botucatu Institute of Biosciences Parasitology DepartmentUNESP Campus Botucatu Institute of Biosciences Parasitology DepartmentDHISão Paulo State Agribusiness Agency TechnologyUniversity of CopenhagenBotanical InstituteUniversidade Estadual Paulista (Unesp)Schlüter, LouiseDavid, Gianmarco S.Jørgensen, Niels O.G.Podduturi, RajuTucci, AndréaDias, Andréa S.da Silva, Reinaldo José [UNESP]2018-12-11T17:19:56Z2018-12-11T17:19:56Z2018-01-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article35-48application/pdfhttp://dx.doi.org/10.3354/AEI00256Aquaculture Environment Interactions, v. 10, p. 35-48.1869-75341869-215Xhttp://hdl.handle.net/11449/17628510.3354/AEI002562-s2.0-850466434332-s2.0-85046643433.pdfScopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengAquaculture Environment Interactions1,034info:eu-repo/semantics/openAccess2023-10-05T06:07:31Zoai:repositorio.unesp.br:11449/176285Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462023-10-05T06:07:31Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Characterization of phytoplankton by pigment analysis and the detection of toxic cyanobacteria in reservoirs with aquaculture production |
title |
Characterization of phytoplankton by pigment analysis and the detection of toxic cyanobacteria in reservoirs with aquaculture production |
spellingShingle |
Characterization of phytoplankton by pigment analysis and the detection of toxic cyanobacteria in reservoirs with aquaculture production Schlüter, Louise Cyanobacteria Fish farm HPLC Microcystins Phytoplankton composition Pigment analysis |
title_short |
Characterization of phytoplankton by pigment analysis and the detection of toxic cyanobacteria in reservoirs with aquaculture production |
title_full |
Characterization of phytoplankton by pigment analysis and the detection of toxic cyanobacteria in reservoirs with aquaculture production |
title_fullStr |
Characterization of phytoplankton by pigment analysis and the detection of toxic cyanobacteria in reservoirs with aquaculture production |
title_full_unstemmed |
Characterization of phytoplankton by pigment analysis and the detection of toxic cyanobacteria in reservoirs with aquaculture production |
title_sort |
Characterization of phytoplankton by pigment analysis and the detection of toxic cyanobacteria in reservoirs with aquaculture production |
author |
Schlüter, Louise |
author_facet |
Schlüter, Louise David, Gianmarco S. Jørgensen, Niels O.G. Podduturi, Raju Tucci, Andréa Dias, Andréa S. da Silva, Reinaldo José [UNESP] |
author_role |
author |
author2 |
David, Gianmarco S. Jørgensen, Niels O.G. Podduturi, Raju Tucci, Andréa Dias, Andréa S. da Silva, Reinaldo José [UNESP] |
author2_role |
author author author author author author |
dc.contributor.none.fl_str_mv |
DHI São Paulo State Agribusiness Agency Technology University of Copenhagen Botanical Institute Universidade Estadual Paulista (Unesp) |
dc.contributor.author.fl_str_mv |
Schlüter, Louise David, Gianmarco S. Jørgensen, Niels O.G. Podduturi, Raju Tucci, Andréa Dias, Andréa S. da Silva, Reinaldo José [UNESP] |
dc.subject.por.fl_str_mv |
Cyanobacteria Fish farm HPLC Microcystins Phytoplankton composition Pigment analysis |
topic |
Cyanobacteria Fish farm HPLC Microcystins Phytoplankton composition Pigment analysis |
description |
The intensified use of water bodies and reservoirs for aquaculture production has increased the need for monitoring and early warning of toxins from cyanobacteria. To minimize effects from toxins, simple and fast analytical monitoring methods are crucial. Here, the content of pigments and microcystins in 14 different strains of cyanobacteria cultured under different growth conditions was investigated to determine the influence of light and nutrient starvation on pigment/ chlorophyll a (chl a) ratios. The obtained pigment/chl a ratios were applied in the software CHEMTAX to calculate the biomass of toxic cyanobacteria, as well as other phytoplankton groups. CHEMTAX ratios from the laboratory cultures were applied to water samples collected during 4 sampling periods at 6 fish farms in different reservoirs in São Paulo State, Brazil. Cyanobacteria generally dominated in all reservoirs in all sampling periods and constituted on average 44 to 66% of the average phytoplankton biomass. The concentrations of microcystins were significantly correlated with the chl a concentrations of cyanobacteria and showed that the pigment method can be used to detect microcystin-producing cyanobacteria in these Brazilian reservoirs. When the concentration of cyanobacteria in the reservoirs was above 4 μg chl a l-1, microcystins were always detected. Our results show that pigment analysis can be used to provide fast and reliable results for the early warning, the presence and potential risk of toxic cyanobacteria in freshwater reservoirs used for aqua culture. |
publishDate |
2018 |
dc.date.none.fl_str_mv |
2018-12-11T17:19:56Z 2018-12-11T17:19:56Z 2018-01-01 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.3354/AEI00256 Aquaculture Environment Interactions, v. 10, p. 35-48. 1869-7534 1869-215X http://hdl.handle.net/11449/176285 10.3354/AEI00256 2-s2.0-85046643433 2-s2.0-85046643433.pdf |
url |
http://dx.doi.org/10.3354/AEI00256 http://hdl.handle.net/11449/176285 |
identifier_str_mv |
Aquaculture Environment Interactions, v. 10, p. 35-48. 1869-7534 1869-215X 10.3354/AEI00256 2-s2.0-85046643433 2-s2.0-85046643433.pdf |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Aquaculture Environment Interactions 1,034 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
35-48 application/pdf |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1797789300332429312 |