Blockade of Rostral Ventrolateral Medulla (RVLM) Bombesin Receptor Type 1 Decreases Blood Pressure and Sympathetic Activity in Anesthetized Spontaneously Hypertensive Rats
Autor(a) principal: | |
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Data de Publicação: | 2016 |
Outros Autores: | , , , , , , , , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.3389/fphys.2016.00205 http://hdl.handle.net/11449/161555 |
Resumo: | Intrathecal injection of bombesin (BBS) promoted hypertensive and sympathoexcitatory effects in normotensive (NT) rats. However, the involvement of rostra' ventrolateral medulla (RVLM) in these responses is still unclear. In the present study, we investigated: (1) the effects of BBS injected bilaterally into RVLM on cardiorespiratory and sympathetic activity in NT and spontaneously hypertensive rats (SHR); (2) the contribution of RVLM BBS type 1 receptors (BB1) to the maintenance of hypertension in SHR. Urethane-anesthetized rats (1.2 g kg(-1), i.v.) were instrumented to record mean arterial pressure (MAP), diaphragm (DIA) motor, and renal sympathetic nerve activity (RSNA). In NT rats and SHR, BBS (0.3 mM) nanoinjected into RVLM increased MAP (33.9 +/- 6.6 and 37.1 +/- 4.5 mmHg, respectively; p 9 0.05) and RSNA (97.8 +/- 12.9 and 84.5 +/- 18.1%, respectively; p < 0.05). In SHR, BBS also increased DIA burst amplitude (115.3 +/- 22.7%; p 0.05). BB1 receptors antagonist (BIM-23127; 3 mM) reduced MAP (-19.9 +/- 4.4 mmHg; p < 0.05) and RSNA (-17.7 +/- 3.8%; p < 0.05) in SHR, but not in NT rats (-2.5 +/- 2.8 mmHg; -2.7 +/- 5.6%, respectively). These results show that BBS can evoke sympathoexcitatory and pressor responses by activating RVLM BB1 receptors. This pathway might be involved in the maintenance of high levels of arterial blood pressure in SHR. |
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Blockade of Rostral Ventrolateral Medulla (RVLM) Bombesin Receptor Type 1 Decreases Blood Pressure and Sympathetic Activity in Anesthetized Spontaneously Hypertensive Ratsrostral ventrolateral medullabombesinBB1 receptorsBIM-23127SHRIntrathecal injection of bombesin (BBS) promoted hypertensive and sympathoexcitatory effects in normotensive (NT) rats. However, the involvement of rostra' ventrolateral medulla (RVLM) in these responses is still unclear. In the present study, we investigated: (1) the effects of BBS injected bilaterally into RVLM on cardiorespiratory and sympathetic activity in NT and spontaneously hypertensive rats (SHR); (2) the contribution of RVLM BBS type 1 receptors (BB1) to the maintenance of hypertension in SHR. Urethane-anesthetized rats (1.2 g kg(-1), i.v.) were instrumented to record mean arterial pressure (MAP), diaphragm (DIA) motor, and renal sympathetic nerve activity (RSNA). In NT rats and SHR, BBS (0.3 mM) nanoinjected into RVLM increased MAP (33.9 +/- 6.6 and 37.1 +/- 4.5 mmHg, respectively; p 9 0.05) and RSNA (97.8 +/- 12.9 and 84.5 +/- 18.1%, respectively; p < 0.05). In SHR, BBS also increased DIA burst amplitude (115.3 +/- 22.7%; p 0.05). BB1 receptors antagonist (BIM-23127; 3 mM) reduced MAP (-19.9 +/- 4.4 mmHg; p < 0.05) and RSNA (-17.7 +/- 3.8%; p < 0.05) in SHR, but not in NT rats (-2.5 +/- 2.8 mmHg; -2.7 +/- 5.6%, respectively). These results show that BBS can evoke sympathoexcitatory and pressor responses by activating RVLM BB1 receptors. This pathway might be involved in the maintenance of high levels of arterial blood pressure in SHR.Fundacao de Amparo a Pesquisa do Estado de Goias (FAPEG)Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)Univ Fed Goias, Dept Physiol Sci, Ctr Neurosci & Cardiovasc Res, Inst Biol Sci, Goiania, Go, BrazilUniv Fed Goias, Fac Pharm, Postgrad Programme Pharmaceut Sci, Goiania, Go, BrazilUniv Fed Goias, Dept Biochem & Mol Biol, Inst Biol Sci, Goiania, Go, BrazilUniv Fed Goias, Dept Morphol, Inst Biol Sci, Goiania, Go, BrazilUniv Fed Uberlandia, Dept Physiol, Coll Phys Educ, Av Engenheiro Dinz 1178,CP 593, BR-38400 Uberlandia, MG, BrazilSao Paulo State Univ, Sch Dent, Dept Physiol & Pathol, Araraquara, BrazilSao Paulo State Univ, Sch Dent, Dept Physiol & Pathol, Araraquara, BrazilFundacao de Amparo a Pesquisa do Estado de Goias (FAPEG): 2012/0055431086Fundacao de Amparo a Pesquisa do Estado de Goias (FAPEG): 2009/10267000352CNPq: 477832/2010-5CNPq: 483411/2012-4CNPq: 447496/2014-0Frontiers Media SaUniversidade Federal de Goiás (UFG)Universidade Federal de Uberlândia (UFU)Universidade Estadual Paulista (Unesp)Pinto, Izabella S.Mourao, Aline A.Silva, Elaine F. daCamargo, Amanda S.Marques, Stefanne M.Gomes, Karina P.Fajemiroye, James O.Silva Reis, Angela A. daRebelo, Ana C. S.Ferreira-Neto, Marcos L.Rosa, Daniel A.Freiria-Oliveira, Andre H.Castro, Carlos H.Colombari, Eduardo [UNESP]Colugnati, Diego B.Pedrino, Gustavo R.2018-11-26T16:33:13Z2018-11-26T16:33:13Z2016-06-02info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article11application/pdfhttp://dx.doi.org/10.3389/fphys.2016.00205Frontiers In Physiology. Lausanne: Frontiers Media Sa, v. 7, 11 p., 2016.1664-042Xhttp://hdl.handle.net/11449/16155510.3389/fphys.2016.00205WOS:000376896400004WOS000376896400004.pdfWeb of Sciencereponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengFrontiers In Physiologyinfo:eu-repo/semantics/openAccess2024-01-23T07:05:44Zoai:repositorio.unesp.br:11449/161555Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-01-23T07:05:44Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Blockade of Rostral Ventrolateral Medulla (RVLM) Bombesin Receptor Type 1 Decreases Blood Pressure and Sympathetic Activity in Anesthetized Spontaneously Hypertensive Rats |
title |
Blockade of Rostral Ventrolateral Medulla (RVLM) Bombesin Receptor Type 1 Decreases Blood Pressure and Sympathetic Activity in Anesthetized Spontaneously Hypertensive Rats |
spellingShingle |
Blockade of Rostral Ventrolateral Medulla (RVLM) Bombesin Receptor Type 1 Decreases Blood Pressure and Sympathetic Activity in Anesthetized Spontaneously Hypertensive Rats Pinto, Izabella S. rostral ventrolateral medulla bombesin BB1 receptors BIM-23127 SHR |
title_short |
Blockade of Rostral Ventrolateral Medulla (RVLM) Bombesin Receptor Type 1 Decreases Blood Pressure and Sympathetic Activity in Anesthetized Spontaneously Hypertensive Rats |
title_full |
Blockade of Rostral Ventrolateral Medulla (RVLM) Bombesin Receptor Type 1 Decreases Blood Pressure and Sympathetic Activity in Anesthetized Spontaneously Hypertensive Rats |
title_fullStr |
Blockade of Rostral Ventrolateral Medulla (RVLM) Bombesin Receptor Type 1 Decreases Blood Pressure and Sympathetic Activity in Anesthetized Spontaneously Hypertensive Rats |
title_full_unstemmed |
Blockade of Rostral Ventrolateral Medulla (RVLM) Bombesin Receptor Type 1 Decreases Blood Pressure and Sympathetic Activity in Anesthetized Spontaneously Hypertensive Rats |
title_sort |
Blockade of Rostral Ventrolateral Medulla (RVLM) Bombesin Receptor Type 1 Decreases Blood Pressure and Sympathetic Activity in Anesthetized Spontaneously Hypertensive Rats |
author |
Pinto, Izabella S. |
author_facet |
Pinto, Izabella S. Mourao, Aline A. Silva, Elaine F. da Camargo, Amanda S. Marques, Stefanne M. Gomes, Karina P. Fajemiroye, James O. Silva Reis, Angela A. da Rebelo, Ana C. S. Ferreira-Neto, Marcos L. Rosa, Daniel A. Freiria-Oliveira, Andre H. Castro, Carlos H. Colombari, Eduardo [UNESP] Colugnati, Diego B. Pedrino, Gustavo R. |
author_role |
author |
author2 |
Mourao, Aline A. Silva, Elaine F. da Camargo, Amanda S. Marques, Stefanne M. Gomes, Karina P. Fajemiroye, James O. Silva Reis, Angela A. da Rebelo, Ana C. S. Ferreira-Neto, Marcos L. Rosa, Daniel A. Freiria-Oliveira, Andre H. Castro, Carlos H. Colombari, Eduardo [UNESP] Colugnati, Diego B. Pedrino, Gustavo R. |
author2_role |
author author author author author author author author author author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade Federal de Goiás (UFG) Universidade Federal de Uberlândia (UFU) Universidade Estadual Paulista (Unesp) |
dc.contributor.author.fl_str_mv |
Pinto, Izabella S. Mourao, Aline A. Silva, Elaine F. da Camargo, Amanda S. Marques, Stefanne M. Gomes, Karina P. Fajemiroye, James O. Silva Reis, Angela A. da Rebelo, Ana C. S. Ferreira-Neto, Marcos L. Rosa, Daniel A. Freiria-Oliveira, Andre H. Castro, Carlos H. Colombari, Eduardo [UNESP] Colugnati, Diego B. Pedrino, Gustavo R. |
dc.subject.por.fl_str_mv |
rostral ventrolateral medulla bombesin BB1 receptors BIM-23127 SHR |
topic |
rostral ventrolateral medulla bombesin BB1 receptors BIM-23127 SHR |
description |
Intrathecal injection of bombesin (BBS) promoted hypertensive and sympathoexcitatory effects in normotensive (NT) rats. However, the involvement of rostra' ventrolateral medulla (RVLM) in these responses is still unclear. In the present study, we investigated: (1) the effects of BBS injected bilaterally into RVLM on cardiorespiratory and sympathetic activity in NT and spontaneously hypertensive rats (SHR); (2) the contribution of RVLM BBS type 1 receptors (BB1) to the maintenance of hypertension in SHR. Urethane-anesthetized rats (1.2 g kg(-1), i.v.) were instrumented to record mean arterial pressure (MAP), diaphragm (DIA) motor, and renal sympathetic nerve activity (RSNA). In NT rats and SHR, BBS (0.3 mM) nanoinjected into RVLM increased MAP (33.9 +/- 6.6 and 37.1 +/- 4.5 mmHg, respectively; p 9 0.05) and RSNA (97.8 +/- 12.9 and 84.5 +/- 18.1%, respectively; p < 0.05). In SHR, BBS also increased DIA burst amplitude (115.3 +/- 22.7%; p 0.05). BB1 receptors antagonist (BIM-23127; 3 mM) reduced MAP (-19.9 +/- 4.4 mmHg; p < 0.05) and RSNA (-17.7 +/- 3.8%; p < 0.05) in SHR, but not in NT rats (-2.5 +/- 2.8 mmHg; -2.7 +/- 5.6%, respectively). These results show that BBS can evoke sympathoexcitatory and pressor responses by activating RVLM BB1 receptors. This pathway might be involved in the maintenance of high levels of arterial blood pressure in SHR. |
publishDate |
2016 |
dc.date.none.fl_str_mv |
2016-06-02 2018-11-26T16:33:13Z 2018-11-26T16:33:13Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.3389/fphys.2016.00205 Frontiers In Physiology. Lausanne: Frontiers Media Sa, v. 7, 11 p., 2016. 1664-042X http://hdl.handle.net/11449/161555 10.3389/fphys.2016.00205 WOS:000376896400004 WOS000376896400004.pdf |
url |
http://dx.doi.org/10.3389/fphys.2016.00205 http://hdl.handle.net/11449/161555 |
identifier_str_mv |
Frontiers In Physiology. Lausanne: Frontiers Media Sa, v. 7, 11 p., 2016. 1664-042X 10.3389/fphys.2016.00205 WOS:000376896400004 WOS000376896400004.pdf |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Frontiers In Physiology |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
11 application/pdf |
dc.publisher.none.fl_str_mv |
Frontiers Media Sa |
publisher.none.fl_str_mv |
Frontiers Media Sa |
dc.source.none.fl_str_mv |
Web of Science reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1799965702109528064 |