Effect of crude glycerine in supplement on the intake, rumen fermentation, and microbial profile of Nellore steers grazing tropical grass
Autor(a) principal: | |
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Data de Publicação: | 2016 |
Outros Autores: | , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1016/j.livsci.2016.08.011 http://hdl.handle.net/11449/173405 |
Resumo: | The objective of this study was to evaluate the effect of crude glycerine (CG) inclusion in feed supplement (0, 70, 140, 210 and 280 g/kg DM basis of supplement) on the forage intake and digestibility, ruminal fermentation parameters, kinetics of fibre degradation, and rumen microbial profile of Nellore steers grazing on tropical grass. Ten ruminally cannulated Nellore steers [490 kg±47 body weight (BW)] were used in a replicated 5×5 Latin square design with 14-d periods. Steers were individually supplemented at the rate of 300 g/100 kg of BW. Inclusion of CG in the supplement did not affect (P>0.05) dry matter intake, apparent total tract digestibility, ruminal pH (P=0.784) or total ruminal VFA (P=0.291), but linearly decreased the NH3-N concentration (P=0.021). The inclusion of CG in the supplement linearly increased (P <0.001) the molar proportions of butyrate and valerate; linearly decreased the acetate (P=0.007) concentration, thus reducing the acetate to propionate ratio (P<0.001); and did not affect the molar proportions of propionate and isobutyrate. Inclusion of GC had a quadratic effect (P=0.010) on the in situ potential degradable fraction of NDF and rate of fibre degradation (P=0.006). Addition of CG linearly decreased the number of protozoa of the genera Entodinium (P=0.015) and Isotricha (P=0.058) and the relative proportions of Ruminococcus albus (P=0.047) and Ruminococcus flavefaciens in the rumen (P=0.036), but did not affect Fibrobacter succinogenes (P=0.420) or the methanogens (P=0.150). The inclusion of CG in the supplement up to 280 g/kg DM altered ruminal fermentation and negatively affected in situ fibre degradation and the gram-positive cellulolytic bacterial population, but did not affect intake and apparent total tract digestibility for Nellore steers grazing tropical grass. |
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Effect of crude glycerine in supplement on the intake, rumen fermentation, and microbial profile of Nellore steers grazing tropical grassBacteriaDegradationForageGlycerolProtozoaThe objective of this study was to evaluate the effect of crude glycerine (CG) inclusion in feed supplement (0, 70, 140, 210 and 280 g/kg DM basis of supplement) on the forage intake and digestibility, ruminal fermentation parameters, kinetics of fibre degradation, and rumen microbial profile of Nellore steers grazing on tropical grass. Ten ruminally cannulated Nellore steers [490 kg±47 body weight (BW)] were used in a replicated 5×5 Latin square design with 14-d periods. Steers were individually supplemented at the rate of 300 g/100 kg of BW. Inclusion of CG in the supplement did not affect (P>0.05) dry matter intake, apparent total tract digestibility, ruminal pH (P=0.784) or total ruminal VFA (P=0.291), but linearly decreased the NH3-N concentration (P=0.021). The inclusion of CG in the supplement linearly increased (P <0.001) the molar proportions of butyrate and valerate; linearly decreased the acetate (P=0.007) concentration, thus reducing the acetate to propionate ratio (P<0.001); and did not affect the molar proportions of propionate and isobutyrate. Inclusion of GC had a quadratic effect (P=0.010) on the in situ potential degradable fraction of NDF and rate of fibre degradation (P=0.006). Addition of CG linearly decreased the number of protozoa of the genera Entodinium (P=0.015) and Isotricha (P=0.058) and the relative proportions of Ruminococcus albus (P=0.047) and Ruminococcus flavefaciens in the rumen (P=0.036), but did not affect Fibrobacter succinogenes (P=0.420) or the methanogens (P=0.150). The inclusion of CG in the supplement up to 280 g/kg DM altered ruminal fermentation and negatively affected in situ fibre degradation and the gram-positive cellulolytic bacterial population, but did not affect intake and apparent total tract digestibility for Nellore steers grazing tropical grass.Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Department of Animal Science College of Agrarian and Veterinarian Sciences (FCAV) Univ Estadual Paulista – UNESPINCT/CA – UFV – Department of Animal ScienceDepartment of Animal Science College of Agrarian and Veterinarian Sciences (FCAV) Univ Estadual Paulista – UNESPFAPESP: 2011/00060-8FAPESP: 2011/06409-2Universidade Estadual Paulista (Unesp)Universidade Federal de Viçosa (UFV)San Vito, Elias [UNESP]Messana, Juliana D. [UNESP]Castagnino, Pablo S. [UNESP]Granja-Salcedo, Yury T. [UNESP]Dallantonia, Erick E. [UNESP]Berchielli, Telma T. [UNESP]2018-12-11T17:05:03Z2018-12-11T17:05:03Z2016-10-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article17-24application/pdfhttp://dx.doi.org/10.1016/j.livsci.2016.08.011Livestock Science, v. 192, p. 17-24.1871-1413http://hdl.handle.net/11449/17340510.1016/j.livsci.2016.08.0112-s2.0-849835512282-s2.0-84983551228.pdfScopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengLivestock Science0,730info:eu-repo/semantics/openAccess2024-01-19T06:31:18Zoai:repositorio.unesp.br:11449/173405Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-01-19T06:31:18Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Effect of crude glycerine in supplement on the intake, rumen fermentation, and microbial profile of Nellore steers grazing tropical grass |
title |
Effect of crude glycerine in supplement on the intake, rumen fermentation, and microbial profile of Nellore steers grazing tropical grass |
spellingShingle |
Effect of crude glycerine in supplement on the intake, rumen fermentation, and microbial profile of Nellore steers grazing tropical grass San Vito, Elias [UNESP] Bacteria Degradation Forage Glycerol Protozoa |
title_short |
Effect of crude glycerine in supplement on the intake, rumen fermentation, and microbial profile of Nellore steers grazing tropical grass |
title_full |
Effect of crude glycerine in supplement on the intake, rumen fermentation, and microbial profile of Nellore steers grazing tropical grass |
title_fullStr |
Effect of crude glycerine in supplement on the intake, rumen fermentation, and microbial profile of Nellore steers grazing tropical grass |
title_full_unstemmed |
Effect of crude glycerine in supplement on the intake, rumen fermentation, and microbial profile of Nellore steers grazing tropical grass |
title_sort |
Effect of crude glycerine in supplement on the intake, rumen fermentation, and microbial profile of Nellore steers grazing tropical grass |
author |
San Vito, Elias [UNESP] |
author_facet |
San Vito, Elias [UNESP] Messana, Juliana D. [UNESP] Castagnino, Pablo S. [UNESP] Granja-Salcedo, Yury T. [UNESP] Dallantonia, Erick E. [UNESP] Berchielli, Telma T. [UNESP] |
author_role |
author |
author2 |
Messana, Juliana D. [UNESP] Castagnino, Pablo S. [UNESP] Granja-Salcedo, Yury T. [UNESP] Dallantonia, Erick E. [UNESP] Berchielli, Telma T. [UNESP] |
author2_role |
author author author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (Unesp) Universidade Federal de Viçosa (UFV) |
dc.contributor.author.fl_str_mv |
San Vito, Elias [UNESP] Messana, Juliana D. [UNESP] Castagnino, Pablo S. [UNESP] Granja-Salcedo, Yury T. [UNESP] Dallantonia, Erick E. [UNESP] Berchielli, Telma T. [UNESP] |
dc.subject.por.fl_str_mv |
Bacteria Degradation Forage Glycerol Protozoa |
topic |
Bacteria Degradation Forage Glycerol Protozoa |
description |
The objective of this study was to evaluate the effect of crude glycerine (CG) inclusion in feed supplement (0, 70, 140, 210 and 280 g/kg DM basis of supplement) on the forage intake and digestibility, ruminal fermentation parameters, kinetics of fibre degradation, and rumen microbial profile of Nellore steers grazing on tropical grass. Ten ruminally cannulated Nellore steers [490 kg±47 body weight (BW)] were used in a replicated 5×5 Latin square design with 14-d periods. Steers were individually supplemented at the rate of 300 g/100 kg of BW. Inclusion of CG in the supplement did not affect (P>0.05) dry matter intake, apparent total tract digestibility, ruminal pH (P=0.784) or total ruminal VFA (P=0.291), but linearly decreased the NH3-N concentration (P=0.021). The inclusion of CG in the supplement linearly increased (P <0.001) the molar proportions of butyrate and valerate; linearly decreased the acetate (P=0.007) concentration, thus reducing the acetate to propionate ratio (P<0.001); and did not affect the molar proportions of propionate and isobutyrate. Inclusion of GC had a quadratic effect (P=0.010) on the in situ potential degradable fraction of NDF and rate of fibre degradation (P=0.006). Addition of CG linearly decreased the number of protozoa of the genera Entodinium (P=0.015) and Isotricha (P=0.058) and the relative proportions of Ruminococcus albus (P=0.047) and Ruminococcus flavefaciens in the rumen (P=0.036), but did not affect Fibrobacter succinogenes (P=0.420) or the methanogens (P=0.150). The inclusion of CG in the supplement up to 280 g/kg DM altered ruminal fermentation and negatively affected in situ fibre degradation and the gram-positive cellulolytic bacterial population, but did not affect intake and apparent total tract digestibility for Nellore steers grazing tropical grass. |
publishDate |
2016 |
dc.date.none.fl_str_mv |
2016-10-01 2018-12-11T17:05:03Z 2018-12-11T17:05:03Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1016/j.livsci.2016.08.011 Livestock Science, v. 192, p. 17-24. 1871-1413 http://hdl.handle.net/11449/173405 10.1016/j.livsci.2016.08.011 2-s2.0-84983551228 2-s2.0-84983551228.pdf |
url |
http://dx.doi.org/10.1016/j.livsci.2016.08.011 http://hdl.handle.net/11449/173405 |
identifier_str_mv |
Livestock Science, v. 192, p. 17-24. 1871-1413 10.1016/j.livsci.2016.08.011 2-s2.0-84983551228 2-s2.0-84983551228.pdf |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Livestock Science 0,730 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
17-24 application/pdf |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
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1797790338705784832 |