Guava Seed Oil: Potential Waste for the Rhamnolipids Production
Autor(a) principal: | |
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Data de Publicação: | 2022 |
Outros Autores: | , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.3390/fermentation8080379 http://hdl.handle.net/11449/242206 |
Resumo: | Guava is consumed in natura and is also of considerable importance to the food industry. The seeds and peel of this fruit are discarded, however, guava seeds yield oil (~13%) that can be used for the bioproducts synthesis. The use of a by-product as a carbon source is advantageous, as it reduces the environmental impact of possible harmful materials to nature, while adding value to products. In addition, the use of untested substrates can bring new yield and characterization results. Thus, this research sought to study rhamnolipids (RLs) production from guava seed oil, a by-product of the fructorefinery. The experiments were carried out using Pseudomonas aeruginosa LBI 2A1 and experimental design was used to optimize the variables Carbon and Nitrogen concentration. Characterization of RLs produced occurred by LC-MS. In this study, variables in the quadratic forms and the interaction between them influenced the response (p < 0.05). The most significant variable was N concentration. Maximum RLs yield achieved 39.97 g/L, predominantly of mono-RL. Characterization analysis revealed 9 homologues including the presence of RhaC10C14:2 (m/z 555) whose structure has not previously been observed. This research showed that guava seed oil is an alternative potential carbon source for rhamnolipid production with rare rhamnolipid homologues. |
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Guava Seed Oil: Potential Waste for the Rhamnolipids ProductionbiosurfactantsC/N ratiocongeners characterizationexperimental designglycolipidsmass spectrometryPseudomonas aeruginosaGuava is consumed in natura and is also of considerable importance to the food industry. The seeds and peel of this fruit are discarded, however, guava seeds yield oil (~13%) that can be used for the bioproducts synthesis. The use of a by-product as a carbon source is advantageous, as it reduces the environmental impact of possible harmful materials to nature, while adding value to products. In addition, the use of untested substrates can bring new yield and characterization results. Thus, this research sought to study rhamnolipids (RLs) production from guava seed oil, a by-product of the fructorefinery. The experiments were carried out using Pseudomonas aeruginosa LBI 2A1 and experimental design was used to optimize the variables Carbon and Nitrogen concentration. Characterization of RLs produced occurred by LC-MS. In this study, variables in the quadratic forms and the interaction between them influenced the response (p < 0.05). The most significant variable was N concentration. Maximum RLs yield achieved 39.97 g/L, predominantly of mono-RL. Characterization analysis revealed 9 homologues including the presence of RhaC10C14:2 (m/z 555) whose structure has not previously been observed. This research showed that guava seed oil is an alternative potential carbon source for rhamnolipid production with rare rhamnolipid homologues.Institute of Biosciences São Paulo State University (Unesp), SPCenter of Natural Sciences Federal University of São Carlos (UFSCar) Lagoa do Sino Campus, SPInstitute for Research in Bioenergy São Paulo State University (Unesp), SPInstitute of Biosciences São Paulo State University (Unesp), SPInstitute for Research in Bioenergy São Paulo State University (Unesp), SPUniversidade Estadual Paulista (UNESP)Universidade Federal de São Carlos (UFSCar)Yoshimura, Ingrid [UNESP]Salazar-Bryam, Ana Maria [UNESP]Faria, Adriano Uemura de [UNESP]Leite, Lucas Prado [UNESP]Lovaglio, Roberta BarrosContiero, Jonas [UNESP]2023-03-02T11:51:11Z2023-03-02T11:51:11Z2022-08-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlehttp://dx.doi.org/10.3390/fermentation8080379Fermentation, v. 8, n. 8, 2022.2311-5637http://hdl.handle.net/11449/24220610.3390/fermentation80803792-s2.0-85136791962Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengFermentationinfo:eu-repo/semantics/openAccess2023-03-02T11:51:12Zoai:repositorio.unesp.br:11449/242206Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462023-03-02T11:51:12Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Guava Seed Oil: Potential Waste for the Rhamnolipids Production |
title |
Guava Seed Oil: Potential Waste for the Rhamnolipids Production |
spellingShingle |
Guava Seed Oil: Potential Waste for the Rhamnolipids Production Yoshimura, Ingrid [UNESP] biosurfactants C/N ratio congeners characterization experimental design glycolipids mass spectrometry Pseudomonas aeruginosa |
title_short |
Guava Seed Oil: Potential Waste for the Rhamnolipids Production |
title_full |
Guava Seed Oil: Potential Waste for the Rhamnolipids Production |
title_fullStr |
Guava Seed Oil: Potential Waste for the Rhamnolipids Production |
title_full_unstemmed |
Guava Seed Oil: Potential Waste for the Rhamnolipids Production |
title_sort |
Guava Seed Oil: Potential Waste for the Rhamnolipids Production |
author |
Yoshimura, Ingrid [UNESP] |
author_facet |
Yoshimura, Ingrid [UNESP] Salazar-Bryam, Ana Maria [UNESP] Faria, Adriano Uemura de [UNESP] Leite, Lucas Prado [UNESP] Lovaglio, Roberta Barros Contiero, Jonas [UNESP] |
author_role |
author |
author2 |
Salazar-Bryam, Ana Maria [UNESP] Faria, Adriano Uemura de [UNESP] Leite, Lucas Prado [UNESP] Lovaglio, Roberta Barros Contiero, Jonas [UNESP] |
author2_role |
author author author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (UNESP) Universidade Federal de São Carlos (UFSCar) |
dc.contributor.author.fl_str_mv |
Yoshimura, Ingrid [UNESP] Salazar-Bryam, Ana Maria [UNESP] Faria, Adriano Uemura de [UNESP] Leite, Lucas Prado [UNESP] Lovaglio, Roberta Barros Contiero, Jonas [UNESP] |
dc.subject.por.fl_str_mv |
biosurfactants C/N ratio congeners characterization experimental design glycolipids mass spectrometry Pseudomonas aeruginosa |
topic |
biosurfactants C/N ratio congeners characterization experimental design glycolipids mass spectrometry Pseudomonas aeruginosa |
description |
Guava is consumed in natura and is also of considerable importance to the food industry. The seeds and peel of this fruit are discarded, however, guava seeds yield oil (~13%) that can be used for the bioproducts synthesis. The use of a by-product as a carbon source is advantageous, as it reduces the environmental impact of possible harmful materials to nature, while adding value to products. In addition, the use of untested substrates can bring new yield and characterization results. Thus, this research sought to study rhamnolipids (RLs) production from guava seed oil, a by-product of the fructorefinery. The experiments were carried out using Pseudomonas aeruginosa LBI 2A1 and experimental design was used to optimize the variables Carbon and Nitrogen concentration. Characterization of RLs produced occurred by LC-MS. In this study, variables in the quadratic forms and the interaction between them influenced the response (p < 0.05). The most significant variable was N concentration. Maximum RLs yield achieved 39.97 g/L, predominantly of mono-RL. Characterization analysis revealed 9 homologues including the presence of RhaC10C14:2 (m/z 555) whose structure has not previously been observed. This research showed that guava seed oil is an alternative potential carbon source for rhamnolipid production with rare rhamnolipid homologues. |
publishDate |
2022 |
dc.date.none.fl_str_mv |
2022-08-01 2023-03-02T11:51:11Z 2023-03-02T11:51:11Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.3390/fermentation8080379 Fermentation, v. 8, n. 8, 2022. 2311-5637 http://hdl.handle.net/11449/242206 10.3390/fermentation8080379 2-s2.0-85136791962 |
url |
http://dx.doi.org/10.3390/fermentation8080379 http://hdl.handle.net/11449/242206 |
identifier_str_mv |
Fermentation, v. 8, n. 8, 2022. 2311-5637 10.3390/fermentation8080379 2-s2.0-85136791962 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Fermentation |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1799965080188616704 |